BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	54,157	C→T	P182P (CCG→CCA)	surA ←	survival protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	54,157	0	C	T	99.5%	641.7 / ‑5.3	182	P182P (CCG→CCA)	surA	survival protein
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (95/86); total (96/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.19e-01

Only 100 of 185 total aligned reads displayed.

GAAATCAGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAG  >  NC_000913/54067‑54246

gAAATCAGCGCCGTTACGCTCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTgg                                                                                         <  2:2774946/93‑1 (MQ=255)

gAAATCAGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTgg                                                                                         <  1:2878809/93‑1 (MQ=255)

 aaaTCAGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTggg                                                                                        <  2:1946118/93‑1 (MQ=255)

 aaaTCAGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTggg                                                                                        >  1:4574192/1‑93 (MQ=255)

   aTCAGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGAt                                                                                      <  1:1552718/93‑1 (MQ=255)

    tCAGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATc                                                                                     <  2:875836/93‑1 (MQ=255)

    tCAGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATc                                                                                     >  2:713579/1‑93 (MQ=255)

     cAGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCa                                                                                    <  1:1047794/93‑1 (MQ=255)

     cAGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCa                                                                                    <  2:2929979/93‑1 (MQ=255)

     cAGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCa                                                                                    <  1:2477502/93‑1 (MQ=255)

      aGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAg                                                                                   >  1:3935019/1‑93 (MQ=255)

          ccGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGAtg                                                                               <  2:43022/93‑1 (MQ=255)

            gTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGAtgtg                                                                             <  1:1868386/93‑1 (MQ=255)

            gTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGAtgtg                                                                             <  1:305583/93‑1 (MQ=255)

             ttACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTgg                                                                            >  2:3317920/1‑93 (MQ=255)

             ttACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTgg                                                                            >  2:4067480/1‑93 (MQ=255)

              tACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGc                                                                           >  2:668292/1‑93 (MQ=255)

              tACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGc                                                                           >  2:633738/1‑93 (MQ=255)

                cgcgCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTc                                                                         <  2:167989/93‑1 (MQ=255)

                 gcgcCTGATCGACAATGGCTCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTcg                                                                        >  1:4110213/1‑92 (MQ=255)

                 gcgcCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCa                                                                        <  2:1112580/93‑1 (MQ=255)

                 gcgcCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCa                                                                        <  2:572602/93‑1 (MQ=255)

                   gcCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAgg                                                                      <  1:4519122/93‑1 (MQ=255)

                    ccTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGt                                                                     >  1:1980675/1‑93 (MQ=255)

                    ccTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGt                                                                     >  2:1315094/1‑93 (MQ=255)

                    ccTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGt                                                                     <  2:3120403/93‑1 (MQ=255)

                    ccTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGt                                                                     >  1:3647599/1‑93 (MQ=255)

                     cTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGCGGCTCAGGtt                                                                    >  1:4220561/1‑93 (MQ=255)

                       gATCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGGTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCa                                                                  >  2:404661/1‑93 (MQ=255)

                        aTCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAg                                                                 >  2:4066677/1‑93 (MQ=255)

                         tCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGc                                                                >  1:1142982/1‑93 (MQ=255)

                         tCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGc                                                                <  1:2548568/93‑1 (MQ=255)

                         tCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGc                                                                <  1:3740381/93‑1 (MQ=255)

                           gACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTc                                                              <  2:2116514/93‑1 (MQ=255)

                           gACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTc                                                              <  2:2276295/93‑1 (MQ=255)

                             cAATGGCGCGCGCCTGTCTTTCGGCTTC‑GTTCACGTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAg                                                            <  2:1807504/93‑1 (MQ=255)

                               aTGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGtc                                                          >  1:1720908/1‑92 (MQ=255)

                               aTGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTg                                                          >  1:765758/1‑93 (MQ=255)

                               aTGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTg                                                          >  2:1204089/1‑93 (MQ=255)

                                  gcgcgcgcCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTg                                                       <  1:904678/93‑1 (MQ=255)

                                   cgcgcgcCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTgg                                                      <  1:2475250/93‑1 (MQ=255)

                                    gcgcgcCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGc                                                     >  1:757767/1‑93 (MQ=255)

                                     cgcgcCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGcg                                                    <  1:2431823/93‑1 (MQ=255)

                                          cTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGtt                                               >  2:3857880/1‑93 (MQ=255)

                                           tGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGttt                                              >  2:2994377/1‑93 (MQ=255)

                                           tGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGttt                                              >  1:4274813/1‑93 (MQ=255)

                                           tGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGttt                                              >  2:1549749/1‑93 (MQ=255)

                                            ggCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGtttt                                             <  1:2120277/93‑1 (MQ=255)

                                             gCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTg                                            >  2:4266723/1‑93 (MQ=255)

                                               tttCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGt                                          <  1:1339881/93‑1 (MQ=255)

                                                ttCCGCCTCGGTTCACCTGAGCAGGGGTCGGGTTTTCCGACAGCGGGATCAGGATGTGGCTCCGGTTGAGCTCAGTGCTGGCGCCGtttggtt                                          <  2:1812972/93‑5 (MQ=255)

                                                 tCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTa                                        <  1:1403383/93‑1 (MQ=255)

                                                  ccGCTTC‑GTTCACCTGATCAGAGGGCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTAc                                       >  2:3275261/1‑93 (MQ=255)

                                                   cGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTAcc                                      <  2:2157643/93‑1 (MQ=255)

                                                    gCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTAccc                                     >  2:4462131/1‑93 (MQ=255)

                                                    gCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTAccc                                     <  2:4627886/93‑1 (MQ=255)

                                                    gCTTC‑GTTCACCTGATCAGAGGTCGGGGTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTAccc                                     >  1:4481977/1‑93 (MQ=255)

                                                     cTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCa                                    >  2:326679/1‑93 (MQ=255)

                                                     cTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCa                                    >  1:2108053/1‑93 (MQ=255)

                                                      ttC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCAc                                   >  1:1830961/1‑93 (MQ=255)

                                                        c‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACct                                 <  2:3031442/93‑1 (MQ=255)

                                                          gTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACctg                                >  2:1283674/1‑93 (MQ=255)

                                                           ttCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACctgc                               >  2:1609469/1‑93 (MQ=255)

                                                           ttCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACctgc                               >  2:3012465/1‑93 (MQ=255)

                                                            tCACCTGGTCAGAGGGCGGGGTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGGTACCCACctgct                              >  2:2249608/1‑93 (MQ=255)

                                                            tCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACctgct                              >  2:491419/1‑93 (MQ=255)

                                                            tCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACctgct                              <  2:1290277/93‑1 (MQ=255)

                                                             cACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACctgctg                             <  1:4404221/93‑1 (MQ=255)

                                                              aCCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCtgctgc                            >  1:1916243/1‑93 (MQ=255)

                                                              aCCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCtgctgc                            >  2:1890827/1‑93 (MQ=255)

                                                              aCCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCtgctgc                            >  1:1545071/1‑93 (MQ=255)

                                                              aCCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCtgctgc                            >  1:3197748/1‑93 (MQ=255)

                                                              aCCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCtgctgc                            <  2:1418435/93‑1 (MQ=255)

                                                               ccTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTgcg                           >  1:1494599/1‑93 (MQ=255)

                                                                cTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTgcgc                          >  2:3444182/1‑93 (MQ=255)

                                                                   aTCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAg                       <  2:1016941/93‑1 (MQ=255)

                                                                   aTCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAg                       <  1:271269/93‑1 (MQ=255)

                                                                    tCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCTACCTTCTGCGCCAgg                      >  1:3422281/1‑93 (MQ=255)

                                                                    tCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAgg                      >  1:913301/1‑93 (MQ=255)

                                                                     cAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAggg                     <  1:3599009/93‑1 (MQ=255)

                                                                      agagGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGa                    <  2:917374/93‑1 (MQ=255)

                                                                      agagGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGa                    <  2:852943/93‑1 (MQ=255)

                                                                       gagGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGAt                   >  2:2528217/1‑93 (MQ=255)

                                                                        agGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGAtt                  >  1:2354501/1‑93 (MQ=255)

                                                                         ggTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTc                 >  1:370826/1‑93 (MQ=255)

                                                                          gTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCg                >  1:3888539/1‑93 (MQ=255)

                                                                          gTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCg                >  1:1000588/1‑93 (MQ=255)

                                                                           tCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGa               >  2:398211/1‑93 (MQ=255)

                                                                           tCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGa               >  1:3346381/1‑93 (MQ=255)

                                                                             gggTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGACt             >  1:159053/1‑93 (MQ=255)

                                                                                ttttCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGACTTcc          >  2:4021166/1‑93 (MQ=255)

                                                                                   tCCGGCAGTGGGATCAGGATGTTGCTCAGGATCAGCTCAGTGCTGGCGTCGTTTTGGTTACTCACCTGCTGCGCCAGGGATTCGACTTCCTGc       >  2:280090/1‑93 (MQ=255)

                                                                                   tCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGACTTCCTGc       <  2:3646712/93‑1 (MQ=255)

                                                                                    ccGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGACTTCCTGCg      <  1:3505032/93‑1 (MQ=255)

                                                                                      ggCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGACTTCCTGCGGc    <  1:462271/93‑1 (MQ=255)

                                                                                      ggCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGACTTCCTGCGGc    >  1:3106907/1‑93 (MQ=255)

                                                                                      ggCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGACTTCCTGCGGc    <  2:144244/93‑1 (MQ=255)

                                                                                      ggCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGACTTCCTGCGGc    <  1:2038070/93‑1 (MQ=255)

                                                                                        cAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAg  <  1:1613753/93‑1 (MQ=255)

                                                                                        cAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAg  <  1:490715/93‑1 (MQ=255)

GAAATCAGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTC‑GTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAG  >  NC_000913/54067‑54246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`