Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 556,959 T→C A2A (GCA→GCG)  folD ← 5,10‑methylene‑tetrahydrofolate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913556,9590TC99.4% 494.9 / ‑4.9 157A2A (GCA→GCG) folD5,10‑methylene‑tetrahydrofolate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (80/76);  total (81/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.08e-01

Only 100 of 167 total aligned reads displayed.

CCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCTGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCGGGGCGCGCTGTCAGGTTTCGTT  >  NC_000913/556869‑557047
                                                                                          |                                                                                        
ccGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTtgccgc                                                                                        >  2:3852291/1‑93 (MQ=255)
  gCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCa                                                                                      <  2:1805044/93‑1 (MQ=255)
     gTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCa                                                                                   >  1:2644706/1‑93 (MQ=255)
      tCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCag                                                                                  <  2:4676679/93‑1 (MQ=255)
        cGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCagag                                                                                <  2:2554547/93‑1 (MQ=255)
         ggCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCagaga                                                                               <  1:3043482/93‑1 (MQ=255)
          gCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCagagag                                                                              >  1:3302187/1‑93 (MQ=255)
           cTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCagagaga                                                                             >  2:2751951/1‑93 (MQ=255)
           cTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCagagaga                                                                             <  2:3225607/93‑1 (MQ=255)
            tGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCagagagag                                                                            >  2:1006576/1‑93 (MQ=255)
             gCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAgg                                                                           >  1:3350802/1‑93 (MQ=255)
             gCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAgg                                                                           <  2:1223080/93‑1 (MQ=255)
              cAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGa                                                                          >  1:600861/1‑93 (MQ=255)
               aaTACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGAt                                                                         >  2:3175198/1‑93 (MQ=255)
               aaTACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGAt                                                                         >  2:1376155/1‑93 (MQ=255)
                aTACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGAtt                                                                        <  2:4043040/93‑1 (MQ=255)
                 tACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTc                                                                       >  2:534168/1‑93 (MQ=255)
                  acccgcCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTTCCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTcc                                                                      <  2:3918267/90‑1 (MQ=255)
                   cgcgCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCa                                                                     <  1:4227944/93‑1 (MQ=255)
                   cgcgCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCa                                                                     <  1:655284/93‑1 (MQ=255)
                      gcCTGAACTTTTTGCGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGCGAGAGGATTCCATCt                                                                  >  1:53435/1‑93 (MQ=255)
                      gcCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCt                                                                  <  1:875192/93‑1 (MQ=255)
                       ccTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTg                                                                 >  2:3549711/1‑93 (MQ=255)
                       ccTGAACTTTTTGAGCAACTTCAGAGCGCACATGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGATAGAGGAGTCCATCTg                                                                 >  2:3788704/1‑93 (MQ=255)
                        cTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGt                                                                >  1:1000778/1‑93 (MQ=255)
                         tGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGtt                                                               <  1:2828648/93‑1 (MQ=255)
                         tGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGtt                                                               >  1:1204005/1‑93 (MQ=255)
                           aaCTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTAc                                                             <  1:2621348/93‑1 (MQ=255)
                             cTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGt                                                           <  1:4399598/93‑1 (MQ=255)
                              tttttGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTa                                                          <  2:2435685/93‑1 (MQ=255)
                              tttttGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTa                                                          >  2:3988964/1‑93 (MQ=255)
                                tttGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGa                                                        <  2:1240907/93‑1 (MQ=255)
                                 ttGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGAt                                                       >  1:4395993/1‑93 (MQ=255)
                                 ttGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGAt                                                       <  1:1045642/93‑1 (MQ=255)
                                  tGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATc                                                      <  1:3980487/93‑1 (MQ=255)
                                    aGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCGATCTGTTACGTAGATCGa                                                    >  2:2216924/1‑93 (MQ=255)
                                     gCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGaa                                                   >  2:2324839/1‑93 (MQ=255)
                                     gCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGaa                                                   >  2:1138053/1‑93 (MQ=255)
                                      cAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAg                                                  <  2:2566134/93‑1 (MQ=255)
                                       aaCTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAgg                                                 >  2:4100700/1‑93 (MQ=255)
                                       aaCTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAgg                                                 <  1:1728671/93‑1 (MQ=255)
                                       aaCTTCAGAGCCCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAgg                                                 >  1:3430099/1‑93 (MQ=255)
                                       aaCGTCAGAGCGCACTTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAgg                                                 <  2:4391355/93‑1 (MQ=255)
                                        aCTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAggg                                                <  1:237486/93‑1 (MQ=255)
                                         cTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAgggg                                               >  1:4638887/1‑93 (MQ=255)
                                          ttCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGa                                              <  1:872400/93‑1 (MQ=255)
                                          ttCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGa                                              <  1:3218716/93‑1 (MQ=255)
                                            cAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATg                                            <  2:1925121/93‑1 (MQ=255)
                                             agagCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATgc                                           <  1:4197080/93‑1 (MQ=255)
                                                gcgcACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGcc                                        >  1:1277263/1‑93 (MQ=255)
                                                  gcctctgctgCGCAATCGTTTTCCCGTCAATAATCTTTCCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCTCCTa                                      <  2:3687729/89‑1 (MQ=255)
                                                  gcACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTa                                      <  1:1931126/93‑1 (MQ=255)
                                                  gcACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTa                                      <  2:1724897/93‑1 (MQ=255)
                                                  gcACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTa                                      >  1:1572952/1‑93 (MQ=255)
                                                  gcACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTa                                      <  1:1091630/93‑1 (MQ=255)
                                                    aCCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTAtt                                    >  2:206627/1‑93 (MQ=255)
                                                     ccTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTAttt                                   <  2:1854855/93‑1 (MQ=255)
                                                     ccTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTAttt                                   >  1:3650527/1‑93 (MQ=255)
                                                      ctgctgCGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTAtttt                                  >  2:641064/1‑93 (MQ=255)
                                                        gctgcGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGt                                <  2:1327428/93‑1 (MQ=255)
                                                        gctgcGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGt                                <  2:3462571/93‑1 (MQ=255)
                                                          tgcGCAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCa                              >  2:3741464/1‑93 (MQ=255)
                                                           gcgcAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAg                             <  1:1833472/93‑1 (MQ=255)
                                                           gcgcAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAg                             <  2:3283887/93‑1 (MQ=255)
                                                           gcgcAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAg                             >  2:2323893/1‑93 (MQ=255)
                                                             gcAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGaa                           <  2:1896794/93‑1 (MQ=255)
                                                             gcAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGaa                           >  1:3501706/1‑93 (MQ=255)
                                                             gcAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGaa                           <  1:2326481/93‑1 (MQ=255)
                                                              cAATCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAg                          >  2:4219262/1‑93 (MQ=255)
                                                               aaTCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGc                         >  1:1920497/1‑93 (MQ=255)
                                                                 tCGTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCgg                       >  2:599475/1‑93 (MQ=255)
                                                                  cGTTTTACCGTCAATAATCTTTTCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCggg                      <  1:2106333/93‑1 (MQ=255)
                                                                   gtgttACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCgggg                     <  1:4164035/90‑1 (MQ=255)
                                                                   gTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCgggg                     >  1:4051243/1‑93 (MQ=255)
                                                                   gTTTTACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCgggg                     >  1:1179649/1‑93 (MQ=255)
                                                                     tttACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCGGGgcg                   >  2:4277394/1‑93 (MQ=255)
                                                                      ttACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCGGGgcgc                  >  1:3583060/1‑93 (MQ=255)
                                                                      ttACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCGGGgcgc                  >  2:3668951/1‑93 (MQ=255)
                                                                       tACCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCGGGgcgcg                 >  1:1184957/1‑93 (MQ=255)
                                                                        aCCGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCGGGgcgcgc                <  1:4505705/93‑1 (MQ=255)
                                                                         ccGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCGGGGCGCGCt               <  2:1548915/93‑1 (MQ=255)
                                                                         ccGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCGGGGCGCGCt               <  1:4331581/93‑1 (MQ=255)
                                                                          cGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCGGGGCGCGCTg              >  1:3211648/1‑93 (MQ=255)
                                                                          cGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCGGGGCGCGCTg              <  2:1402111/93‑1 (MQ=255)
                                                                          cGTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCGGGGCGCGCTg              >  1:2568493/1‑93 (MQ=255)
                                                                           gTCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCGGGGCGCGCTGt             <  1:1780586/93‑1 (MQ=255)
                                                                            tCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCGGGGCGCGCTGTc            >  2:1017127/1‑93 (MQ=255)
                                                                            tCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCGGGGCGCGCTGTc            <  1:3813192/93‑1 (MQ=255)
                                                                            tCAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCGGGGCGCGCTGTc            >  1:4473933/1‑93 (MQ=255)
                                                                             cAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCGGGGCGCGCTGTCa           <  1:3083004/93‑1 (MQ=255)
                                                                             cAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCGGGGCGCGCTGTCa           <  2:3073757/93‑1 (MQ=255)
                                                                             cAATAATCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCGGGGCGCGCTGTCa           >  2:3277100/1‑93 (MQ=255)
                                                                              aataatCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCGGGGCGCGCTGTCAg          <  1:44325/93‑1 (MQ=255)
                                                                              aataatCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCGGGGCGCGCTGTCAg          >  2:2139019/1‑93 (MQ=255)
                                                                               ataatCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGCTGCGCCTATTTTGTCAGAAGCGGGGCGCGCTGTCAgg         >  1:2685799/1‑93 (MQ=255)
                                                                                 aatCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCGGGGCGCGCTGTCAGgtt       >  1:1361283/1‑93 (MQ=255)
                                                                                  atCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCGGGGCGCGCTGTCAGgttt      >  2:1488596/1‑93 (MQ=255)
                                                                                   tCTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCGGGGCGCGCTGTCAGgtttc     >  2:4417412/1‑93 (MQ=255)
                                                                                    cTTTGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCGAGGCGCGCTGTCAGgtttcg    <  2:4552032/93‑1 (MQ=255)
                                                                                     tttGCCGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCGGGGCGCGCTGTCAGgtttcgt   <  2:1087169/93‑1 (MQ=255)
                                                                                          |                                                                                        
CCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCTGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCGGGGCGCGCTGTCAGGTTTCGTT  >  NC_000913/556869‑557047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑