Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 627,609 A→G E231E (GAA→GAG)  entB → 2,3‑dihydro‑2,3‑dihydroxybenzoate synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913627,6090AG94.6% 464.5 / 11.5 167E231E (GAA→GAG) entB2,3‑dihydro‑2,3‑dihydroxybenzoate synthetase
Reads supporting (aligned to +/- strand):  ref base A (4/5);  new base G (79/78);  total (83/84)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 171 total aligned reads displayed.

GATGACTGAAGAATTACTGCCAGCACCTATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGA  >  NC_000913/627519‑627698
                                                                                          |                                                                                         
gatgaCTGAAGAATTACTGCCAGCACCTATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGcc                                                                                         >  2:2933276/1‑93 (MQ=255)
  tgaatgaaGAATTACTGCCAGCACCTATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGt                                                                                       <  1:3595666/93‑1 (MQ=255)
   gaCTGAAGAATTACTGCCAGCACCTATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGtt                                                                                      <  2:2579744/93‑1 (MQ=255)
   gaCTGAAAAATTACTGCCAGCACCTATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGtt                                                                                      <  1:2420744/93‑1 (MQ=255)
    acggaagaaTTACTCCCAGCACTTACCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTc                                                                                     <  2:3032848/90‑1 (MQ=255)
     cTGAAGAATTACTGCCAGCACCTATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCg                                                                                    >  1:2335465/1‑93 (MQ=255)
      tGAAGAATTACTGCCAGAACCTATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGa                                                                                   >  1:4635612/1‑93 (MQ=255)
       gaagaaTTACTGCCAGCACCTATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGAt                                                                                  >  1:1191374/1‑93 (MQ=255)
        aagaaTTACTGCCAGCACCTATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATg                                                                                 <  1:1477108/93‑1 (MQ=255)
        aaaaaTTTCTCCCAGCCCTTACCACCGCCAGCAAAGCGGCGCTGCGTGAGGTTATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATg                                                                                 <  2:2106242/90‑1 (MQ=255)
         agaaTTACTGCCAGCACCTATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATga                                                                                >  1:2701033/1‑93 (MQ=255)
          gaaTTCCTGCCAGCACCTATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATgac                                                                               <  1:2535823/93‑1 (MQ=255)
          gaaTTACTGCCAGCACCTATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATgac                                                                               <  1:1878435/93‑1 (MQ=255)
          aaaTTACTGCCAGCACCTATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATgac                                                                               <  2:1755928/92‑1 (MQ=255)
            aTTACTGCCAGCACCTATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATgacga                                                                             >  1:2625954/1‑93 (MQ=255)
             ttACTGCCAGCACCTATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATgacgac                                                                            >  1:1568312/1‑93 (MQ=255)
             ttACTGCCAGCACCTATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATgacgac                                                                            >  2:457044/1‑93 (MQ=255)
              tACTGCCAGCACCTATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACa                                                                           >  2:2251127/1‑93 (MQ=255)
               aCTGCCAGCACCTATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACaa                                                                          >  2:331167/1‑93 (MQ=255)
                 tGCCAGCACCTATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAAcc                                                                        >  1:3623457/1‑93 (MQ=255)
                 tGCAAGCACATATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAAcc                                                                        <  1:545215/93‑1 (MQ=255)
                  gCCAGCACCTATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCt                                                                       >  2:461489/1‑93 (MQ=255)
                  gCCAGCACCTATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCt                                                                       <  2:714618/93‑1 (MQ=255)
                    cAGCACCTATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGCTGAGCCGTTCGATGACGACAACCTgt                                                                     >  2:1881633/1‑92 (MQ=255)
                    cAGCACCTATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGa                                                                     >  2:1128427/1‑93 (MQ=255)
                    cAGCACCTACCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGa                                                                     <  1:1687370/93‑1 (MQ=255)
                        cccTACCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGa                                                                 <  1:4144924/92‑1 (MQ=255)
                         cttATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGAc                                                                <  1:204421/91‑1 (MQ=255)
                         ccTATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGAc                                                                >  1:3333023/1‑93 (MQ=255)
                         ccTACCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGAc                                                                <  1:1231499/93‑1 (MQ=255)
                          cTATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACt                                                               >  2:4143384/1‑93 (MQ=255)
                          cTATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACt                                                               >  2:3628243/1‑93 (MQ=255)
                            tcccccGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTAc                                                             <  2:540145/91‑1 (MQ=255)
                            ctCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTAc                                                             <  2:4399594/92‑1 (MQ=255)
                            acccccGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTAc                                                             <  1:1025383/91‑1 (MQ=255)
                             tCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACg                                                            <  1:46681/93‑1 (MQ=255)
                             tCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACg                                                            <  1:4533658/93‑1 (MQ=255)
                             tCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACg                                                            >  1:1104552/1‑93 (MQ=255)
                             tCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACg                                                            >  1:880998/1‑93 (MQ=255)
                              ccccGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACgg                                                           <  2:3090228/93‑1 (MQ=255)
                               cccGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGt                                                          <  2:2382771/93‑1 (MQ=255)
                                   ccAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTgg                                                      >  1:2064743/1‑93 (MQ=255)
                                    cAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGa                                                     >  1:794106/1‑93 (MQ=255)
                                     aGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGAt                                                    >  2:4536598/1‑93 (MQ=255)
                                     aGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGAt                                                    >  2:3527936/1‑93 (MQ=255)
                                       cAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTc                                                  <  1:347254/93‑1 (MQ=255)
                                        aaaGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCg                                                 >  2:2340041/1‑93 (MQ=255)
                                        aaaGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCg                                                 >  1:2690799/1‑93 (MQ=255)
                                         aaGCGGGGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCgg                                                <  2:477741/93‑1 (MQ=255)
                                         aaGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCgg                                                >  2:1592758/1‑93 (MQ=255)
                                           gcggcgCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTg                                              >  1:2195313/1‑93 (MQ=255)
                                           gcggcgCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTg                                              <  1:1116126/93‑1 (MQ=255)
                                            gggcgCTGGGTGAGGTGATCCTGCCGTTGCGGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTgc                                             <  1:1186494/92‑1 (MQ=255)
                                             ggcgCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTgcg                                            >  2:2480066/1‑93 (MQ=255)
                                                gcTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCat                                         >  1:914257/1‑93 (MQ=255)
                                                gcTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCat                                         >  1:2493472/1‑93 (MQ=255)
                                                gcTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCat                                         >  2:814170/1‑93 (MQ=255)
                                                 cTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCatg                                        >  1:3380415/1‑93 (MQ=255)
                                                  tGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCatga                                       <  1:3583439/93‑1 (MQ=255)
                                                  tGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCatga                                       <  2:15148/93‑1 (MQ=255)
                                                  tGCGTGAGGTGATCCTGCCGTGGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCatga                                       <  1:2404067/93‑1 (MQ=255)
                                                  gtggTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCatga                                       <  1:2593750/90‑1 (MQ=255)
                                                   gCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCatgat                                      >  1:2040969/1‑93 (MQ=255)
                                                       gAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGgcg                                  <  1:1687080/93‑1 (MQ=255)
                                                        aGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGgcgc                                 >  2:4337442/1‑93 (MQ=255)
                                                        aGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGgcgc                                 <  1:4346333/93‑1 (MQ=255)
                                                        aGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGgcgc                                 <  2:907185/93‑1 (MQ=255)
                                                        aGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGgcgc                                 <  2:139936/93‑1 (MQ=255)
                                                        aGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGgcgc                                 <  2:3444924/93‑1 (MQ=255)
                                                         ggTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCt                                <  1:4361286/93‑1 (MQ=255)
                                                         ggTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCt                                <  2:4006347/93‑1 (MQ=255)
                                                         ggTGACCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACATCCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCt                                <  2:4267018/93‑1 (MQ=255)
                                                          gTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTg                               >  2:2202165/1‑93 (MQ=255)
                                                          gTGATCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTg                               >  1:484309/1‑93 (MQ=255)
                                                             aTCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTggcg                            <  2:1438427/93‑1 (MQ=255)
                                                              tCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTggcgg                           >  1:884534/1‑93 (MQ=255)
                                                              tCCTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTggcgg                           >  1:2740267/1‑93 (MQ=255)
                                                               ccTGCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTggcggc                          <  2:2725670/93‑1 (MQ=255)
                                                                cTGCCGGTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGgcggcg                         <  1:1577609/93‑1 (MQ=255)
                                                                  gCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGgcgcg                       >  1:3502604/1‑93 (MQ=255)
                                                                  gCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGgcgcg                       <  1:1434669/93‑1 (MQ=255)
                                                                  gCCGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGgcgcg                       >  2:3807456/1‑93 (MQ=255)
                                                                    cGTTGCTGGACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCt                     <  1:688705/93‑1 (MQ=255)
                                                                     gTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTg                    <  2:2720577/93‑1 (MQ=255)
                                                                           ggACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCaa              >  1:3545238/1‑93 (MQ=255)
                                                                           ggACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCaa              <  1:2944196/93‑1 (MQ=255)
                                                                            gACGAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCaaa             <  1:2888884/93‑1 (MQ=255)
                                                                               gAGTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTg          <  1:3225030/93‑1 (MQ=255)
                                                                                 gTCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCa        >  2:1443460/1‑93 (MQ=255)
                                                                                  tCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCAt       <  2:2612417/93‑1 (MQ=255)
                                                                                  tCCGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCAt       <  1:363052/93‑1 (MQ=255)
                                                                                   ccGATGAGCCGTTCGATGACGACATCCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATg      >  2:2603704/1‑93 (MQ=255)
                                                                                   ccGATGAGCCGTGCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATg      <  2:4355785/93‑1 (MQ=255)
                                                                                    cGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGGGCGCTGGCGCCAAGTGCATgg     >  2:121665/1‑93 (MQ=255)
                                                                                    cGATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATgg     >  2:1987131/1‑93 (MQ=255)
                                                                                     gATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGt    <  2:2797981/93‑1 (MQ=255)
                                                                                     gATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGt    >  1:628925/1‑93 (MQ=255)
                                                                                     gATGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGt    <  2:1239143/93‑1 (MQ=255)
                                                                                       tGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGa  >  2:2737036/1‑93 (MQ=255)
                                                                                       tGAGCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGa  <  1:3963608/93‑1 (MQ=255)
                                                                                          |                                                                                         
GATGACTGAAGAATTACTGCCAGCACCTATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGA  >  NC_000913/627519‑627698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑