BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	631,938	C→T	A255T (GCA→ACA)	ybdH ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	631,938	0	C	T	99.2%	474.0 / NA	134	A255T (GCA→ACA)	ybdH	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (59/74); minor base G (0/1); total (59/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

ACCAGAATTCCGTAGGCGACTTTGGTGCCGTGGAGAAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGCCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATCCACCACATCGCAAAATGATTGCGTT  >  NC_000913/631850‑632026

aCCAGAATTCCGTAGGCGACTTTGGTGCCGTGGAGAAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTgtcgcc                                                                                      >  1:4186318/1‑93 (MQ=255)

  cAGAATTCCGTAGGCGACTTTGGTGCCGTGGAGAAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCcac                                                                                    <  1:3731183/93‑1 (MQ=255)

  cAGAATTCCGTAGGCGACTTTGGTGCCGTGGAGAAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCcac                                                                                    >  1:2018885/1‑93 (MQ=255)

   aGAATGCCGTAGGCGACTTTGGTGCCGTGGAGAAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCcaca                                                                                   <  1:2119154/93‑1 (MQ=255)

    gAATTCCGTAGGCGACTTTGGTGCCGTGGAGAAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCacac                                                                                  >  1:1246353/1‑93 (MQ=255)

    gAATTCCGTAGGCGACTTTGGTGCCGTGGAGAAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCacac                                                                                  <  2:2826259/93‑1 (MQ=255)

    gAATTCCGTAGGCGACTTTGGTGCCGTGGAGAAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCacac                                                                                  >  2:4457976/1‑93 (MQ=255)

       ttCCGTAGGCGACTTTGGTGCCGTGGAGAAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACAcgcg                                                                               >  2:975760/1‑93 (MQ=255)

         ccGTAGGCGACTTTGGTGCCGTGGAGAAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTa                                                                             <  2:3755879/93‑1 (MQ=255)

          cGTAGGCGACTTTGGTGCCGTGGAGAAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTaa                                                                            >  1:764335/1‑93 (MQ=255)

            tAGGCGACTTTGGTGCCGTGGAGAAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTaaaa                                                                          >  1:3372375/1‑93 (MQ=255)

            tAGGCGACTTTGGTGCCGTGGAGAAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTaaaa                                                                          <  1:203871/93‑1 (MQ=255)

            tAGGCGACTTTGGTGCCGTGGAGAAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTaaaa                                                                          >  2:2872310/1‑93 (MQ=255)

             aGGCGACTTTGGTGCCGTGGAGAAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAAc                                                                         <  2:3500905/93‑1 (MQ=255)

             aGGCGACTTTGGTGCCGTGGAGAAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAAc                                                                         <  1:1260980/93‑1 (MQ=255)

              ggCGACTTTGGTGCCGTGGAGAAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACGCGCGTAAAACg                                                                        <  2:4054613/93‑1 (MQ=255)

               gCGTCTTTGGTGCCGTGGAGAAACTTCTCGGTTTGCGGCAGCACGGTCAAACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGa                                                                       <  1:1203979/93‑1 (MQ=255)

                cGACTTTGGTGCCGTGGAGAAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGAt                                                                      <  2:4515816/93‑1 (MQ=255)

                 gACTTTGGTGCCGTGGAGAAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATc                                                                     <  2:1807003/93‑1 (MQ=255)

                 gACTTTGGTGCCGTGGAGAAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATc                                                                     <  1:615019/93‑1 (MQ=255)

                 gACTTTGGTGCCGTGGAGAAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATc                                                                     <  1:4460889/93‑1 (MQ=255)

                 gACTTTGGTGCCGTGGAGAAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATc                                                                     >  1:4015857/1‑93 (MQ=255)

                  aCTTTGGTGCCGTGGAGAAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCg                                                                    <  1:1276182/93‑1 (MQ=255)

                  aCTTTGGTGCCGTGGAGAAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCg                                                                    >  1:561905/1‑93 (MQ=255)

                  aCTTTGGTGCCGTGGAGAAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCg                                                                    >  2:360978/1‑93 (MQ=255)

                  aCTTTGGTGCCGTGGAGAAACTACTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCg                                                                    <  1:4547168/93‑1 (MQ=255)

                   cTTTGGTGCCGTGGAGAAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGc                                                                   >  2:2784064/1‑93 (MQ=255)

                    tttGGTGCCGTGGAGAAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGcc                                                                  >  1:4282455/1‑93 (MQ=255)

                        gTGCCGTGGAGAAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGa                                                              <  1:1353675/93‑1 (MQ=255)

                           ccGGGGAGAAACTTCTCGGCTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGGCCAGacta                                                           >  2:2302150/1‑91 (MQ=255)

                            cGTGGAGAAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGaccac                                                          <  1:1431218/93‑1 (MQ=255)

                                  gaAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCaccaacca                                                    >  1:369502/1‑93 (MQ=255)

                                  gaAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCaccaacca                                                    <  1:4276333/93‑1 (MQ=255)

                                  gaAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCaccaacca                                                    >  1:4370962/1‑93 (MQ=255)

                                  gaAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCaccaacca                                                    >  1:3203793/1‑93 (MQ=255)

                                  gaAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCaccaacca                                                    >  1:3123780/1‑93 (MQ=255)

                                  gaAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCaccaacca                                                    <  1:2989271/93‑1 (MQ=255)

                                   aaaCTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCAt                                                   <  1:1786330/93‑1 (MQ=255)

                                    aaCTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATc                                                  >  1:4644913/1‑93 (MQ=255)

                                    aaCTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGGCGCCACACGCGTAAAACGATCGCCCAGACCCCCAACCATc                                                  <  1:672916/93‑1 (MQ=255)

                                     aCTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATcc                                                 >  1:4539027/1‑93 (MQ=255)

                                      cTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCCACCATccc                                                >  1:1985530/1‑93 (MQ=255)

                                      cTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATccc                                                >  1:4601648/1‑93 (MQ=255)

                                      cTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCAACAAACATccc                                                >  2:351093/1‑93 (MQ=255)

                                       ttCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCcca                                               <  2:4355539/93‑1 (MQ=255)

                                        tctcGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCccac                                              <  2:710514/93‑1 (MQ=255)

                                         ctcGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCccacc                                             >  1:693333/1‑93 (MQ=255)

                                         ctcGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCccaca                                             >  1:3652720/1‑92 (MQ=255)

                                             gTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCccaccacca                                         <  1:1608824/93‑1 (MQ=255)

                                                tGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCa                                      >  2:1670573/1‑93 (MQ=255)

                                                tGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCa                                      <  1:1400898/93‑1 (MQ=255)

                                                tGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCa                                      <  1:3823159/93‑1 (MQ=255)

                                                 gCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCaa                                     <  2:1911800/93‑1 (MQ=255)

                                                   ggCAGCACGGTCAGACCGTTATGCACGGCATGATCTGTCGCCACACGCGTAAAACGATCGCCCACACCACCAACCATCCCCCCACCAGCaata                                   <  1:663798/93‑1 (MQ=255)

                                                   ggCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCaata                                   <  1:2095801/93‑1 (MQ=255)

                                                     cagcaCGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCaataat                                 <  1:1093952/93‑1 (MQ=255)

                                                      agcaCGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAataata                                <  2:1309370/93‑1 (MQ=255)

                                                      agcaCGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAataata                                <  2:3303732/93‑1 (MQ=255)

                                                        caCGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGc                              <  1:3939341/93‑1 (MQ=255)

                                                        caCGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGc                              <  1:129443/93‑1 (MQ=255)

                                                         aCGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAGAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCa                             >  2:3804049/1‑93 (MQ=255)

                                                         aCGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCa                             >  1:271218/1‑93 (MQ=255)

                                                         aCGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCa                             <  1:1539288/93‑1 (MQ=255)

                                                         aCGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCa                             >  2:3980903/1‑93 (MQ=255)

                                                         aCGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCa                             >  2:4520405/1‑93 (MQ=255)

                                                          cGGTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCAt                            <  1:4039517/93‑1 (MQ=255)

                                                           ggTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATc                           >  1:3867800/1‑93 (MQ=255)

                                                            gTCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATcc                          <  1:502325/93‑1 (MQ=255)

                                                             tCAGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATcca                         <  1:1800586/93‑1 (MQ=255)

                                                               aGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATccacc                       >  1:4149546/1‑93 (MQ=255)

                                                               aGACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATccacc                       <  1:3064870/93‑1 (MQ=255)

                                                                gACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATccacca                      <  2:3194556/93‑1 (MQ=255)

                                                                gACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATccacca                      >  2:2764100/1‑93 (MQ=255)

                                                                gACCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATccacca                      >  2:860440/1‑93 (MQ=255)

                                                                 aCCGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATCcaccac                     >  1:1416081/1‑93 (MQ=255)

                                                                  ccGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATCCACcaca                    <  2:4274360/93‑1 (MQ=255)

                                                                  ccGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATCCACcaca                    <  1:125719/93‑1 (MQ=255)

                                                                   cGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATCCACCACAt                   <  1:1191850/93‑1 (MQ=255)

                                                                   cGTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATCCACCACAt                   >  1:3658168/1‑93 (MQ=255)

                                                                    gTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATCCACCACATc                  >  1:4557879/1‑93 (MQ=255)

                                                                    gTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATCCACCACATc                  <  2:851646/93‑1 (MQ=255)

                                                                    gTTATGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATCCACCACATc                  <  2:1048233/93‑1 (MQ=255)

                                                                        tGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATCCACCACATCGCaa              >  2:803378/1‑93 (MQ=255)

                                                                        tGCACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATCCACCACATCGCaa              >  1:1694414/1‑93 (MQ=255)

                                                                          cACGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATCCACCACATCGCaaaa            <  1:4235083/93‑1 (MQ=255)

                                                                           aCGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATCCACCACATCGCAAAAt           <  2:1831277/93‑1 (MQ=255)

                                                                           aCGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATACCACCACCAGCAATAATAGCATCCACCACATCGCAAAAt           <  1:2734526/93‑1 (MQ=255)

                                                                            cGGCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATCCACCACATCGCAAAATg          <  2:1024061/93‑1 (MQ=255)

                                                                              gCATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATCCACCACATCGCAAAATGAt        <  1:2184031/93‑1 (MQ=255)

                                                                               cATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATCCACCACATCGCAAAATGAtt       <  1:920331/93‑1 (MQ=255)

                                                                               cATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATCCACCACATCGCAAAATGAtt       <  2:3100747/93‑1 (MQ=255)

                                                                               cATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATCCACCACATCGCAAAATGAtt       <  2:4322526/93‑1 (MQ=255)

                                                                               cATGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATCCACCACATCGCAAAATGAtt       <  1:2954695/93‑1 (MQ=255)

                                                                                aTGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATCCACCACATCGCAAAATGATTg      >  2:4133820/1‑93 (MQ=255)

                                                                                 tGAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATCCACCACATCGCAAAATGATTGc     >  1:4496907/1‑93 (MQ=255)

                                                                                  gAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATCCACCACATCGCAAAATGATTGCg    >  1:1747274/1‑93 (MQ=255)

                                                                                  gAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATCCACCACATCGCAAAATGATTGCg    >  1:8616/1‑93 (MQ=255)

                                                                                  gAGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATCCACCACATCGCAAAATGATTGCg    <  2:3997452/93‑1 (MQ=255)

                                                                                   aGCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATCCACCACATCGCAAAATGATTGCGt   <  2:2803996/93‑1 (MQ=255)

                                                                                    gCTGTCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATCCACCACATCGCAAAATGATTGCGtt  >  2:1524237/1‑93 (MQ=255)

ACCAGAATTCCGTAGGCGACTTTGGTGCCGTGGAGAAACTTCTCGGTTTGCGGCAGCACGGTCAGACCGTTATGCACGGCATGAGCTGCCGCCACACGCGTAAAACGATCGCCCAGACCACCAACCATCCCACCACCAGCAATAATAGCATCCACCACATCGCAAAATGATTGCGTT  >  NC_000913/631850‑632026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`