BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	862,272	T→C	T164A (ACT→GCT)	ybiY ←	putative pyruvate formate‑lyase activating enzyme 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	862,272	0	T	C	100.0%	416.4 / NA	133	T164A (ACT→GCT)	ybiY	putative pyruvate formate‑lyase activating enzyme 2
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (82/51); total (82/51)

Only 100 of 136 total aligned reads displayed.

CGTGTTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAACCGC  >  NC_000913/862182‑862361

cGTGTTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCAt                                                                                         <  1:932791/93‑1 (MQ=255)

  tgtTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGa                                                                                       <  1:2519705/93‑1 (MQ=255)

   gtTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGaa                                                                                      <  1:3095280/93‑1 (MQ=255)

   gtTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGaa                                                                                      <  1:2881726/93‑1 (MQ=255)

   gtTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGaa                                                                                      <  1:2603739/93‑1 (MQ=255)

   gtTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGaa                                                                                      <  1:4385334/93‑1 (MQ=255)

    ttttAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAAt                                                                                     <  2:1861847/93‑1 (MQ=255)

     tttAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAAtg                                                                                    >  2:1708540/1‑93 (MQ=255)

     tttAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAAtg                                                                                    >  1:38735/1‑93 (MQ=255)

      ttAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAAtgc                                                                                   <  1:123824/93‑1 (MQ=255)

       tAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAAtgcc                                                                                  <  2:3111993/93‑1 (MQ=255)

         aaTCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAAtgcctg                                                                                <  2:1235734/93‑1 (MQ=255)

            cGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACACGCAGACAGGTTTCTACCGCAGCATGAATgcctgcct                                                                             <  1:13082/93‑1 (MQ=255)

              gCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCg                                                                           >  1:1967224/1‑93 (MQ=255)

              gCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCg                                                                           >  2:2427689/1‑93 (MQ=255)

               cAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGt                                                                          <  2:3494537/93‑1 (MQ=255)

                aaGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGtg                                                                         <  2:529029/93‑1 (MQ=255)

                aaGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGtg                                                                         >  2:1296014/1‑93 (MQ=255)

                  gAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGtggc                                                                       >  2:1650661/1‑93 (MQ=255)

                  gAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGtggc                                                                       >  1:3394102/1‑93 (MQ=255)

                    aaCAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGtggctg                                                                     <  1:2218972/93‑1 (MQ=255)

                    aaCAGAGCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGtggctg                                                                     <  2:832626/93‑1 (MQ=255)

                      cAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGtggctggc                                                                   <  1:2210961/93‑1 (MQ=255)

                        gATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCtt                                                                 >  2:2897345/1‑93 (MQ=255)

                        gATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCtt                                                                 >  2:2705161/1‑93 (MQ=255)

                        gATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCtt                                                                 >  1:3716827/1‑93 (MQ=255)

                         aTCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTg                                                                >  2:3845955/1‑93 (MQ=255)

                         aTCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTg                                                                >  1:2303909/1‑93 (MQ=255)

                         aTCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTg                                                                >  1:1704806/1‑93 (MQ=255)

                          tCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTATCGCAGCATGAATGCCTGCCTCGTGGCTGGCtttt                                                               >  2:1381602/1‑91 (MQ=255)

                          tCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGc                                                               <  2:3294637/93‑1 (MQ=255)

                           cGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCa                                                              <  1:1637536/93‑1 (MQ=255)

                           cGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCa                                                              <  1:1402258/93‑1 (MQ=255)

                           cGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCa                                                              <  2:2404030/93‑1 (MQ=255)

                            gATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAg                                                             >  1:3372195/1‑93 (MQ=255)

                             atatAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGt                                                            >  2:3860340/1‑93 (MQ=255)

                              tataGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTa                                                           <  1:3429146/93‑1 (MQ=255)

                              tataGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTa                                                           >  1:3641091/1‑93 (MQ=255)

                               ataGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAg                                                          <  2:895998/93‑1 (MQ=255)

                               ataGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAg                                                          >  2:1176769/1‑93 (MQ=255)

                               ataGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAg                                                          <  2:1664688/93‑1 (MQ=255)

                                  gggCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAgcgc                                                       >  2:3653996/1‑93 (MQ=255)

                                   ggCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgcc                                                      >  2:1778790/1‑93 (MQ=255)

                                      agagAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatc                                                   >  1:2352179/1‑93 (MQ=255)

                                      agagAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatc                                                   >  2:1603559/1‑93 (MQ=255)

                                      agagAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatc                                                   <  2:4549047/93‑1 (MQ=255)

                                       gagaAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatcg                                                  >  1:779658/1‑93 (MQ=255)

                                       gagaAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatcg                                                  >  1:834011/1‑93 (MQ=255)

                                       gagaAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatcg                                                  >  2:2832838/1‑93 (MQ=255)

                                         gaAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatcgcc                                                >  2:4287145/1‑93 (MQ=255)

                                         gaAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatcgcc                                                <  1:3565221/93‑1 (MQ=255)

                                         gaAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatcgcc                                                >  1:3487875/1‑93 (MQ=255)

                                           aGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatcgccat                                              <  1:4660150/93‑1 (MQ=255)

                                           aGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatcgccat                                              >  2:4362133/1‑93 (MQ=255)

                                           aGGGGCGATATATTTCCACGGCACATGCAGACAGGGTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatcgccat                                              >  1:4618637/1‑93 (MQ=255)

                                            ggggCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCAtt                                             >  1:2656593/1‑93 (MQ=255)

                                             gggCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCAttt                                            >  2:131846/1‑93 (MQ=255)

                                             gggCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCAttt                                            >  2:302087/1‑93 (MQ=255)

                                               gCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTcc                                          >  1:3934600/1‑93 (MQ=255)

                                                cGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCg                                         <  2:1834784/93‑1 (MQ=255)

                                                  atatatTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGc                                       >  2:2317532/1‑93 (MQ=255)

                                                  atatatTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGc                                       >  1:160046/1‑93 (MQ=255)

                                                  atatatTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGc                                       >  2:3857826/1‑93 (MQ=255)

                                                  atatatTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGc                                       >  2:275659/1‑93 (MQ=255)

                                                  atatatTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCCCCATCGCCATTTCCGGc                                       >  1:3368425/1‑93 (MQ=255)

                                                   tatatTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCt                                      >  2:1071947/1‑93 (MQ=255)

                                                      atTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCa                                   >  2:10794/1‑93 (MQ=255)

                                                      atTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCa                                   >  1:545965/1‑93 (MQ=255)

                                                       tttCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCAt                                  >  2:2913400/1‑93 (MQ=255)

                                                       tttCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCAt                                  >  1:69721/1‑93 (MQ=255)

                                                       tttCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCAt                                  >  1:1902502/1‑93 (MQ=255)

                                                         tCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATaa                                >  1:69757/1‑93 (MQ=255)

                                                          ccACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATaaa                               <  1:30173/93‑1 (MQ=255)

                                                          ccACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATaaa                               <  1:515017/93‑1 (MQ=255)

                                                           cACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAg                              <  1:1671506/93‑1 (MQ=255)

                                                           cACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAg                              <  2:135052/93‑1 (MQ=255)

                                                           cACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAg                              >  2:2819427/1‑93 (MQ=255)

                                                            aCGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAgg                             >  2:1727248/1‑93 (MQ=255)

                                                             cGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAggg                            >  2:3728692/1‑93 (MQ=255)

                                                                 acaTGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTca                        >  2:4468487/1‑93 (MQ=255)

                                                                 acaTGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTca                        >  1:269482/1‑93 (MQ=255)

                                                                 acaTGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTca                        >  1:1096569/1‑93 (MQ=255)

                                                                  caTGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTTGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTcac                       >  1:1069603/1‑93 (MQ=255)

                                                                   aTGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTcacc                      <  2:2139811/93‑1 (MQ=255)

                                                                     gCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTcaccac                    >  2:121040/1‑93 (MQ=255)

                                                                      cAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCaccacc                   <  2:324930/93‑1 (MQ=255)

                                                                      cAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCaccacc                   >  1:2023246/1‑93 (MQ=255)

                                                                        gACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGGAGTAGCGCCATCGCCATTTGCGGCTGCATAAAGGGCCCAcccccca                 >  1:1135197/1‑92 (MQ=255)

                                                                        gACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCCTTTCCGGCTGCATAAAGGGCTCACCACCCg                 >  1:4594139/1‑93 (MQ=255)

                                                                            ggTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAg             >  1:1082974/1‑93 (MQ=255)

                                                                            ggTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAg             >  1:505917/1‑93 (MQ=255)

                                                                             gTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGTCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGc            <  1:975828/93‑1 (MQ=255)

                                                                             gTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGc            <  1:1476112/93‑1 (MQ=255)

                                                                               ttCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGGAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGt          <  1:3538581/93‑1 (MQ=255)

                                                                               ttCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGt          <  2:3451492/93‑1 (MQ=255)

                                                                                tCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGtt         >  1:4027193/1‑93 (MQ=255)

                                                                                  tACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTaa       <  2:2529564/93‑1 (MQ=255)

                                                                                  tACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTaa       >  2:4312476/1‑93 (MQ=255)

                                                                                  tACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGGTaa       >  2:3403161/1‑93 (MQ=255)

                                                                                       cAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAAccgc  <  2:1624890/93‑1 (MQ=255)

CGTGTTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAACCGC  >  NC_000913/862182‑862361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`