BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	934,839	T→C	V798A (GTT→GCT)	ftsK →	cell division protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	934,839	0	T	C	99.1%	320.1 / NA	110	V798A (GTT→GCT)	ftsK	cell division protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (67/41); total (67/43)
Fisher's exact test for biased strand distribution p-value = 3.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 125 total aligned reads displayed.

CACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGTTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGTAATGG  >  NC_000913/934749‑934929

cacaCCAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTg                                                                                          <  1:3999262/93‑1 (MQ=255)

cacaACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTg                                                                                          >  2:1129272/1‑93 (MQ=255)

    acaGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGcgcc                                                                                      >  1:2319707/1‑93 (MQ=255)

    acaGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGcgcc                                                                                      >  2:674751/1‑93 (MQ=255)

       gCCGGTTGGCCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCgca                                                                                   <  2:3808409/93‑1 (MQ=255)

       gCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGGCgca                                                                                   >  2:621424/1‑93 (MQ=255)

       gCCGGTGGCGCCCCAGCCACAATAGCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCgca                                                                                   <  1:1310146/93‑1 (MQ=18)

          ggTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTGGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCgcagca                                                                                >  1:2507144/1‑93 (MQ=255)

             tGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCgcagcagca                                                                             <  1:3996672/93‑1 (MQ=255)

                 ccgcAGCCCAAAAATCCGCAGCCGCAACCACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTAt                                                                         <  1:550243/93‑1 (MQ=18)

                 ccgcAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTAt                                                                         >  2:2547481/1‑93 (MQ=255)

                  cgcAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATc                                                                        <  1:1819821/93‑1 (MQ=255)

                  cgcAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATc                                                                        >  2:594786/1‑93 (MQ=255)

                   gcAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATca                                                                       >  1:1014508/1‑93 (MQ=255)

                   gcAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATca                                                                       >  1:1895928/1‑93 (MQ=255)

                   gcAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATca                                                                       >  1:3873391/1‑93 (MQ=255)

                         acaATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGcc                                                                 >  1:1297919/1‑93 (MQ=255)

                         acaATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGcc                                                                 >  2:496463/1‑93 (MQ=255)

                         acaATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGcc                                                                 >  2:697718/1‑93 (MQ=255)

                           aaTATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCcac                                                               <  1:4561886/93‑1 (MQ=255)

                            atatCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCcaca                                                              >  1:1737272/1‑93 (MQ=255)

                            atatCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCcaca                                                              <  2:3897202/93‑1 (MQ=255)

                             tatCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCacaa                                                             >  2:436526/1‑93 (MQ=255)

                              atCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCacaac                                                            <  1:4444248/93‑1 (MQ=255)

                              atCACCACCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCacaac                                                            <  2:1196770/93‑1 (MQ=17)

                               tCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCacaaca                                                           >  2:2307522/1‑93 (MQ=255)

                               tCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCacaaca                                                           >  2:1410748/1‑93 (MQ=255)

                               acagcagCCCCAGCAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCacaaca                                                           <  2:1007424/92‑1 (MQ=17)

                                cggcaccCGGATCATCCGGTCGCGCCACAGCCGCAATATCAGCAGCCGCAACAGCCGGCTGCGCCGCAGCGGCAGTATCAGCAGCCACAACAg                                                          <  2:573536/91‑1 (MQ=14)

                                  gcagcCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGcc                                                        >  1:1195709/1‑93 (MQ=255)

                                  gaagcCGCAACAACCTGTTGCGCCACAGCAGCAATATCAGCAGCCACAACAAACGGCTGCGCCGCAGCAGCAGTATCAGCAGCCCCAACATcc                                                        >  2:2322776/3‑93 (MQ=17)

                                  ccagcCGCAACACCCGGTGGCCCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGcc                                                        <  2:1779427/92‑1 (MQ=14)

                                   cagcCGCAACATCTTGATCCGCTACATCATCAATAACAGCAGCCGGAACAACCGGGAGCACCGCAGCAGCAGTAACAGCAGCCACAACAGCCa                                                       <  1:4473730/93‑1 (MQ=12)

                                   cacccGCAATAACCGGTTCCCCCCCAGCAGCAATATCAGCAGCCGCAACACCCGGCTACGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCa                                                       <  2:721348/90‑1 (MQ=14)

                                      ccGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGtt                                                    <  2:3617971/93‑1 (MQ=255)

                                        gCAACACCCGATTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTgc                                                  <  2:2551051/93‑1 (MQ=255)

                                        gCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTgc                                                  >  1:2910079/1‑93 (MQ=255)

                                        gCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTgc                                                  >  1:2236625/1‑93 (MQ=255)

                                          aacaacCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTgcgc                                                >  1:2855600/1‑93 (MQ=255)

                                            caacGGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGTTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCca                                              <  2:3087435/93‑1 (MQ=255)

                                              gccGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCcaca                                            >  2:4472242/2‑93 (MQ=255)

                                              acCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCcaca                                            <  1:4108817/93‑1 (MQ=255)

                                              acCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCcaca                                            >  2:2924557/1‑93 (MQ=255)

                                              acCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCcaca                                            >  1:3654881/1‑93 (MQ=255)

                                               ccGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACaa                                           >  1:546700/1‑93 (MQ=255)

                                                cGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAAc                                          <  1:921558/93‑1 (MQ=255)

                                                cGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAAc                                          <  1:3214141/93‑1 (MQ=255)

                                                 ggTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAAcc                                         >  1:815768/1‑93 (MQ=255)

                                                  gTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCg                                        >  2:3755433/1‑93 (MQ=255)

                                                  gTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCg                                        >  1:3184622/1‑93 (MQ=255)

                                                  gTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCAACAACAGCCAGTTGCGCCACAACCg                                        >  2:3870486/1‑93 (MQ=255)

                                                       cccACAGAAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGa                                   <  1:1226057/92‑1 (MQ=255)

                                                        ccACAGCAGCAATATCAGCAGCCGCACCACCCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGAt                                  <  1:3194453/93‑1 (MQ=255)

                                                        ccACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGac                                  >  1:3752076/1‑92 (MQ=255)

                                                        ccACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGATGCGCCACAACCGCAGGAt                                  >  1:1495652/1‑93 (MQ=255)

                                                         cacaGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATa                                 >  2:3632367/1‑93 (MQ=255)

                                                          ccagcagCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATAc                                <  2:2716951/92‑1 (MQ=255)

                                                          acacCAGCAATATCACCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCACCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATAc                                <  2:3589293/93‑1 (MQ=21)

                                                          acaGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATAc                                >  1:1406771/1‑93 (MQ=255)

                                                          acaGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATAc                                >  2:1553369/1‑93 (MQ=255)

                                                            agcagcAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATAccc                              >  2:500687/1‑93 (MQ=255)

                                                            agcagcAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATAccc                              <  2:2590046/93‑1 (MQ=255)

                                                            agcagcAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATAccc                              >  1:4684297/1‑93 (MQ=255)

                                                              cagcaATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTg                            >  2:2080435/1‑93 (MQ=255)

                                                               acaaaTATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGc                           <  2:245923/90‑1 (MQ=255)

                                                                  aaTATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTc                        <  1:432849/93‑1 (MQ=255)

                                                                  aaTATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTc                        >  2:1012781/1‑93 (MQ=255)

                                                                   atatCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCa                       >  1:1561254/1‑93 (MQ=255)

                                                                   atacCACCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCa                       <  2:3018270/93‑1 (MQ=255)

                                                                    tatCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCAt                      >  1:90168/1‑93 (MQ=255)

                                                                     atCAGCAGCCGCAACAACGGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATc                     <  2:337097/93‑1 (MQ=255)

                                                                     accggcagcCGCAACAACCGGCTGCGCCGCAGTAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATc                     <  2:2797948/89‑1 (MQ=25)

                                                                      tCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATcc                    >  2:2401959/1‑93 (MQ=255)

                                                                      tCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATcc                    >  2:427203/1‑93 (MQ=255)

                                                                      tCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATcc                    >  2:2226392/1‑93 (MQ=255)

                                                                         gcagcCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCt                 >  1:1088977/1‑93 (MQ=255)

                                                                         gcagcCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCt                 <  1:2931881/93‑1 (MQ=255)

                                                                         gcagcCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCt                 >  1:917340/1‑93 (MQ=255)

                                                                         gcagcCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCt                 >  1:4062530/1‑93 (MQ=255)

                                                                           agcCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGt               <  1:4340035/93‑1 (MQ=255)

                                                                           agcCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGt               <  2:526953/93‑1 (MQ=255)

                                                                           agcCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGt               >  2:445216/1‑93 (MQ=255)

                                                                            gcCGCAACACCCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGtt              <  2:1704471/93‑1 (MQ=255)

                                                                            gcCGCAACACCCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGtt              <  2:4067865/93‑1 (MQ=255)

                                                                            gcCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGtt              >  2:3381977/1‑93 (MQ=255)

                                                                            gcCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGtt              >  1:2798744/1‑93 (MQ=255)

                                                                            cccGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGtt              <  2:472247/92‑1 (MQ=255)

                                                                              cGCAACAACCGGCGGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGa            <  2:1637406/93‑1 (MQ=255)

                                                                               gCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCCCAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGAt           >  2:540148/1‑93 (MQ=255)

                                                                                caacaaCCGGTGGCGCGGTAGCAGAACTAACAGCAGCCACAGCAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATg          <  2:1948280/93‑1 (MQ=255)

                                                                                caacaaCCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATg          <  1:1256807/93‑1 (MQ=255)

                                                                                 aacaccCGGTTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGc         <  2:3002096/93‑1 (MQ=255)

                                                                                 aacaacCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATTCGCTGTTGATGc         >  2:4645533/1‑93 (MQ=255)

                                                                                 aacaacCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGc         >  1:1166990/1‑93 (MQ=255)

                                                                                 aacaacCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGc         >  2:185650/1‑93 (MQ=255)

                                                                                 aacaacCGGCGGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGc         <  1:252260/93‑1 (MQ=255)

                                                                                    aacCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGTa      >  1:3222816/1‑93 (MQ=255)

                                                                                       cGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGTAATg   >  1:1883819/1‑93 (MQ=255)

                                                                                       cGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGTAATg   >  1:3409324/1‑93 (MQ=255)

                                                                                        ggTTGCGCCGCAGCAGCAGTATCAGCAGCCTCAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGTAATgg  <  1:1453556/93‑1 (MQ=255)

CACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGTTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGTAATGG  >  NC_000913/934749‑934929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`