Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 83,384 A→G V49A (GTC→GCC)  leuA ← 2‑isopropylmalate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091383,3840AG99.4% 485.6 / ‑4.6 157V49A (GTC→GCC) leuA2‑isopropylmalate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (65/91);  total (65/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01

Only 100 of 166 total aligned reads displayed.

TTTCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGT  >  NC_000913/83295‑83473
                                                                                         |                                                                                         
tttCCACGCAGCGAGCTGACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCgg                                                                                        <  2:1265319/93‑1 (MQ=255)
tttCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCgg                                                                                        <  2:2848377/93‑1 (MQ=255)
tttCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCgg                                                                                        <  2:811133/93‑1 (MQ=255)
 ttCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCggg                                                                                       <  2:2874744/93‑1 (MQ=255)
  tCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCgggg                                                                                      <  2:2534122/93‑1 (MQ=255)
     aCGCAGCGAGCTAACGCCCATACGCGTCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGaaa                                                                                   <  1:1150938/93‑1 (MQ=255)
       gCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAAcc                                                                                 >  1:4242045/1‑93 (MQ=255)
            gAGCTACCGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACtt                                                                            <  2:1671250/93‑1 (MQ=255)
             aGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTc                                                                           <  1:3721829/93‑1 (MQ=255)
             aGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAAACGACTTc                                                                           >  2:1722628/1‑93 (MQ=255)
               cTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCa                                                                         <  1:353654/93‑1 (MQ=255)
                tAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGCAGAGGCGGGGAAACCGACTTCCac                                                                        >  2:3536270/1‑92 (MQ=255)
                tAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCAt                                                                        >  1:204894/1‑93 (MQ=255)
                 aaCGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATc                                                                       <  2:773109/93‑1 (MQ=255)
                 aaCGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATc                                                                       >  1:3092234/1‑93 (MQ=255)
                 aaCGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATc                                                                       >  1:2525481/1‑93 (MQ=255)
                  aCGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCa                                                                      <  1:1359835/93‑1 (MQ=255)
                  aCGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCa                                                                      >  1:713772/1‑93 (MQ=255)
                    gCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACg                                                                    <  1:2993347/93‑1 (MQ=255)
                      acaTACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTc                                                                  >  2:3149097/1‑93 (MQ=255)
                      acaTACGCGGCTGTTTTTAACCTGGCGGGCGATGGGTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGAGAAACCGACTTtcatcaagcc                                                                  >  1:6985/1‑89 (MQ=255)
                        aTACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCaa                                                                <  2:2624679/93‑1 (MQ=255)
                        aTACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCaa                                                                >  1:2896738/1‑93 (MQ=255)
                         tACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAac                                                               <  2:1439154/93‑1 (MQ=255)
                         tACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAac                                                               <  2:2762094/93‑1 (MQ=255)
                         tACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAac                                                               >  1:2001431/1‑93 (MQ=255)
                           cgcgGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGGAACCGACTTCCATCACGTCAacac                                                             >  1:4019250/1‑93 (MQ=255)
                           cgcgGCTGTTTTTAACCTGGCGGGCGATGGTTCGCACCGATTCAAAATCGTCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAacac                                                             <  1:3296387/93‑1 (MQ=255)
                              ggCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCa                                                          <  1:1128919/93‑1 (MQ=255)
                              ggCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCa                                                          <  2:2347098/93‑1 (MQ=255)
                               gCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGGCTTCCATCACGTCAACAcccct                                                         >  2:3486050/1‑91 (MQ=255)
                                cTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATa                                                        <  1:3499847/93‑1 (MQ=255)
                                cTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATa                                                        <  1:3324621/93‑1 (MQ=255)
                                cTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATa                                                        <  2:3429742/93‑1 (MQ=255)
                                cTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATa                                                        <  1:4653440/93‑1 (MQ=255)
                                 tGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCTACACCCATAc                                                       <  2:2868560/93‑1 (MQ=255)
                                 tGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATAc                                                       <  2:827258/93‑1 (MQ=255)
                                 tGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATAc                                                       <  2:3133211/93‑1 (MQ=255)
                                 tGTTTTTAACCGGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATAc                                                       <  2:4395505/93‑1 (MQ=255)
                                  gTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACg                                                      >  1:1500045/1‑93 (MQ=255)
                                  gTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACg                                                      >  1:198845/1‑93 (MQ=255)
                                    ttttAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCt                                                    <  1:4023843/93‑1 (MQ=255)
                                    ttttAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCt                                                    <  2:1558814/93‑1 (MQ=255)
                                     tttAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACCTCAACACCCCAACGCTc                                                   >  2:3814495/1‑93 (MQ=255)
                                     tttAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAAACGACTTCCATCACGTCAACACCCATACGCTc                                                   >  2:455623/1‑93 (MQ=255)
                                       tAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCaa                                                 <  2:469256/93‑1 (MQ=255)
                                       tAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCaa                                                 <  2:2470513/93‑1 (MQ=255)
                                        aaCCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCCACACCCATACGCTCAAg                                                >  1:650519/1‑93 (MQ=255)
                                        aaCCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAg                                                <  2:1453871/93‑1 (MQ=255)
                                        aaCCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAg                                                <  1:10087/93‑1 (MQ=255)
                                         aCCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAgg                                               <  1:4171026/93‑1 (MQ=255)
                                           cTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGc                                             >  1:2296105/1‑93 (MQ=255)
                                            tGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGcc                                            <  1:837933/93‑1 (MQ=255)
                                            tGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGcc                                            <  2:1627865/93‑1 (MQ=255)
                                            tGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGcc                                            >  1:3978260/1‑93 (MQ=255)
                                            tGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGGCAACACCCATACGATCAAGTGcc                                            >  2:1937164/1‑93 (MQ=255)
                                             ggcgggcgATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCa                                           <  1:1539764/93‑1 (MQ=255)
                                              gcgggcgATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAg                                          <  2:791101/93‑1 (MQ=255)
                                               cgggcgATGGTTTGCACCGATTCAAAAGCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAgc                                         <  2:1525914/93‑1 (MQ=255)
                                                   ggATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCaa                                     <  2:1759857/92‑1 (MQ=255)
                                                   cgATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCaa                                     >  1:894716/1‑93 (MQ=255)
                                                   cgATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCaa                                     <  1:2322352/93‑1 (MQ=255)
                                                    gATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAAt                                    >  2:1309647/1‑93 (MQ=255)
                                                     aTGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGGAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAAtt                                   >  2:663260/1‑93 (MQ=255)
                                                     aTGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAAtt                                   <  1:1589832/93‑1 (MQ=255)
                                                     aTGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAAtt                                   <  2:898274/93‑1 (MQ=255)
                                                     aTGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAAtt                                   <  2:3345057/93‑1 (MQ=255)
                                                         tttGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCa                               <  2:3720648/93‑1 (MQ=255)
                                                           tGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGt                             >  1:4309825/1‑93 (MQ=255)
                                                           tGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGt                             <  2:4243923/93‑1 (MQ=255)
                                                              aCCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGtttt                          >  1:3101493/1‑93 (MQ=255)
                                                              aCCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGtttt                          <  2:2726245/93‑1 (MQ=255)
                                                              aCCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGtttt                          <  1:1664612/93‑1 (MQ=255)
                                                              aCCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGtttt                          <  2:415433/93‑1 (MQ=255)
                                                              aCCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATAAGCTCAAGGGCCAGCCCAATTTGGAGtttt                          >  1:2146582/1‑93 (MQ=255)
                                                               ccGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGttttt                         >  2:2571842/1‑93 (MQ=255)
                                                               ccGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGttttt                         >  2:1598266/1‑93 (MQ=255)
                                                                 gATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTtttct                       <  1:3006728/93‑1 (MQ=255)
                                                                 gATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGGTtttct                       >  1:1009759/1‑93 (MQ=255)
                                                                 gATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCCCAAGGGCCAGCGCAATTTGCAGTTtttct                       >  1:3074033/1‑93 (MQ=255)
                                                                  aTTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTtttctt                      <  1:4152407/93‑1 (MQ=255)
                                                                    tCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTtttctttc                    <  1:4549507/93‑1 (MQ=255)
                                                                     cAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTca                   <  1:4545678/93‑1 (MQ=255)
                                                                        aaTCGCCCGGCGGAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCacac                <  2:884633/93‑1 (MQ=255)
                                                                        aaTCGCCCGGCGAAGAGGCGGGGGAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCacac                >  2:939418/1‑93 (MQ=255)
                                                                        aaTCGCCCGGCGAAGAGACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCacac                <  2:4659161/93‑1 (MQ=255)
                                                                         aTCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACt               >  2:1848236/1‑93 (MQ=255)
                                                                           cGCCCTGAGAACAGGCGGTGAAACCGAATTCCATCCCGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCa             <  1:1299427/93‑1 (MQ=255)
                                                                           cGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCa             <  2:2345361/93‑1 (MQ=255)
                                                                            gCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCaa            <  2:2257476/93‑1 (MQ=255)
                                                                             cccGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAg           <  2:4252786/93‑1 (MQ=255)
                                                                             cccGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAg           <  1:634808/93‑1 (MQ=255)
                                                                             cccGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAg           <  1:2563122/93‑1 (MQ=255)
                                                                             cccGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAg           <  1:1401340/93‑1 (MQ=255)
                                                                              ccGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGc          >  1:727518/1‑93 (MQ=255)
                                                                                   gAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCTGTTTTTCTTTCACACTCCAGCTTGcc     >  2:816449/1‑93 (MQ=255)
                                                                                   gAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGcc     >  2:2689230/1‑93 (MQ=255)
                                                                                   gAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGcc     <  2:121594/93‑1 (MQ=255)
                                                                                      gaggCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGt  >  1:3130365/1‑93 (MQ=255)
                                                                                      gaggCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGGTTTTCTTTCACACTCAAGCTTGGCTGt  >  1:76789/1‑93 (MQ=255)
                                                                                         |                                                                                         
TTTCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGT  >  NC_000913/83295‑83473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑