BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	996,175	A→G	G187G (GGT→GGC)	ycbP ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	996,175	0	A	G	99.2%	383.6 / ‑4.9	123	G187G (GGT→GGC)	ycbP	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (65/57); total (66/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01

Only 100 of 126 total aligned reads displayed.

GCTTCAGGCGAGGATTCTGCAGCAACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTACCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAATGCCTGCCAGAAAGTTTCTAGCGCGGTATCAAGACGGGTTTG  >  NC_000913/996085‑996264

gCTTCAGGCGAGGATTCTGCAGCAACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGcc                                                                                         >  2:2665001/1‑93 (MQ=255)

gCTTCAGGCGAGGATTCTGCAGCAACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGcc                                                                                         <  1:2440444/93‑1 (MQ=255)

gCTTCAGGCGAGGATTCTGCAGCAACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGcc                                                                                         <  2:4499782/93‑1 (MQ=255)

 cgtCAGGCGAGGATTCTCCAGCAACCTCAAACGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCt                                                                                        <  1:199296/91‑1 (MQ=255)

 cTTCAGGCGAGGATTCTGCAGCAACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCt                                                                                        <  2:2760799/93‑1 (MQ=255)

 cTTCAGGCGAGGATTCTGCAGCAACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCt                                                                                        <  1:4398008/93‑1 (MQ=255)

 cTTCAGGCGAGGATTCTGCAGCAACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCt                                                                                        >  1:1271066/1‑93 (MQ=255)

 cTTCAGGCGAGGATTCTGCAGCAACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCt                                                                                        >  1:2163221/1‑93 (MQ=255)

  ttCAGGCGAGGATTCTGCAGCAACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTc                                                                                       <  1:842928/93‑1 (MQ=255)

  ttCAGGCGAGGATTCTGCAGCAACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTc                                                                                       >  1:3576966/1‑93 (MQ=255)

  ttCAGGCGAGGATTCTGCAGCAACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTc                                                                                       <  2:491342/93‑1 (MQ=255)

  ttCAGGCGAGGATTCTGCAGCAACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTc                                                                                       <  1:450715/93‑1 (MQ=255)

     aGGCGAGGATTCTGCAGCAACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCa                                                                                    <  1:1959962/93‑1 (MQ=255)

      ggCGAGGATTCTGCAGCAACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCag                                                                                   >  1:3959854/1‑93 (MQ=255)

        cGAGGATTCTGCAGCAACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCagag                                                                                 >  2:357078/1‑93 (MQ=255)

         gAGGATTCTGCAGCAACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAgaga                                                                                <  1:4313565/93‑1 (MQ=255)

          aGGATTCTGCAGCAACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGAc                                                                               >  2:713451/1‑93 (MQ=255)

          aGGATTCTGCAGCAACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGAc                                                                               <  1:3391082/93‑1 (MQ=255)

           ggATTCTGCAGCAACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACa                                                                              <  1:2760209/93‑1 (MQ=255)

           ggATTCTGCAGCAACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACa                                                                              >  2:1028249/1‑93 (MQ=255)

           ggATTCTGCAGCAACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACa                                                                              >  1:4560193/1‑93 (MQ=255)

            gATTCTGCAGCAACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAg                                                                             >  1:1206433/1‑93 (MQ=255)

            gATTCTGCAGCAACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAg                                                                             >  1:4269347/1‑93 (MQ=255)

             aTTCTGCAGCAACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGa                                                                            <  2:4506267/93‑1 (MQ=255)

             aTTCTGCAGCAACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGa                                                                            <  1:1993836/93‑1 (MQ=255)

              ttCTGCAGCAACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGaa                                                                           <  2:3249526/93‑1 (MQ=255)

               tcggcagcaACCGCAAAAGTAGAGACCCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAg                                                                          <  1:864336/90‑1 (MQ=255)

               tCTGCAGCAACCGCAAAAGCAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAg                                                                          >  2:4570766/1‑93 (MQ=255)

                cTGCAGCAACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAgg                                                                         <  2:1178051/93‑1 (MQ=255)

                  gcagcaACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTc                                                                       <  2:2063520/93‑1 (MQ=255)

                   cagcaACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCa                                                                      >  2:3246370/1‑93 (MQ=255)

                   cagcaACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCa                                                                      <  1:4292740/93‑1 (MQ=255)

                   cagcaACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCa                                                                      <  2:1428987/93‑1 (MQ=255)

                    agcaACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAg                                                                     <  1:3582764/93‑1 (MQ=255)

                     gcaACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAgg                                                                    >  2:1496016/1‑93 (MQ=255)

                       aaCCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGaa                                                                  >  2:4129127/1‑93 (MQ=255)

                        aCCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAAc                                                                 >  2:898179/1‑93 (MQ=255)

                         ccGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAAcc                                                                >  2:3471601/1‑93 (MQ=255)

                         ccGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAAcc                                                                >  2:45982/1‑93 (MQ=255)

                         ccGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAAcc                                                                <  2:2901360/93‑1 (MQ=255)

                           gCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTg                                                              >  2:3199975/1‑93 (MQ=255)

                              aaaGTAGAGAGTCGAAGCACTCCCGCAAGCTCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAAc                                                           <  2:918352/93‑1 (MQ=255)

                              aaaGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAAc                                                           >  2:4183939/1‑93 (MQ=255)

                              aaaGCAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGACGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAAc                                                           <  2:2522603/93‑1 (MQ=255)

                                aGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACAt                                                         <  2:1962189/93‑1 (MQ=255)

                                 gTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATc                                                        >  2:2090788/1‑93 (MQ=255)

                                   agagACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATcgc                                                      >  1:4349367/1‑93 (MQ=255)

                                    gagaCGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATcgcg                                                     <  2:513621/93‑1 (MQ=255)

                                    gagaCGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATcgcg                                                     <  2:1864880/93‑1 (MQ=255)

                                      gaCGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCgcggc                                                   >  2:2661439/1‑93 (MQ=255)

                                        cGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGcggcgg                                                 >  1:579439/1‑93 (MQ=255)

                                        cGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGcggcgg                                                 >  2:2719629/1‑93 (MQ=255)

                                        cGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGcggcgg                                                 <  1:2491323/93‑1 (MQ=255)

                                        cGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGcggcgg                                                 <  1:2130448/93‑1 (MQ=255)

                                           tAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGc                                              <  1:4014600/93‑1 (MQ=255)

                                            aaGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCa                                             >  2:3959242/1‑93 (MQ=255)

                                             aGCACTCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCaa                                            <  1:1636641/93‑1 (MQ=255)

                                             aGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCat                                            >  2:3569815/1‑92 (MQ=255)

                                             aGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCaa                                            <  1:2714578/93‑1 (MQ=255)

                                              gCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAAt                                           <  1:698922/93‑1 (MQ=255)

                                               cgaTCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAAtg                                          >  2:2957126/3‑93 (MQ=255)

                                                aaTCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAAtgc                                         >  2:2998791/1‑93 (MQ=255)

                                                 aTCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAAtgcc                                        >  1:2169710/1‑93 (MQ=255)

                                                 aTCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAAtgcc                                        >  1:1149926/1‑93 (MQ=255)

                                                 aTCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAAtgcc                                        >  1:3697961/1‑93 (MQ=255)

                                                 aTCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTACCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAAtgcc                                        >  1:2853306/1‑93 (MQ=255)

                                                  tCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAAtgcct                                       <  1:4003820/93‑1 (MQ=255)

                                                  tCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAAtgcct                                       <  2:4371074/93‑1 (MQ=255)

                                                    ccGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAAtgcctgc                                     >  2:2489323/1‑93 (MQ=255)

                                                     cgccagcgccagTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAAtgcctgcc                                    <  1:1629101/93‑1 (MQ=255)

                                                        cagcgccagTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAATGCCTGCCAGa                                 >  2:3625994/1‑93 (MQ=255)

                                                          gcgccagTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAATGCCTGCCAGaaa                               <  1:2938029/93‑1 (MQ=255)

                                                          gcgccagTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAATGCCTGCCAGaaa                               >  2:1621806/1‑93 (MQ=255)

                                                          gcgccagTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAATGCCTGCCAGaaa                               <  2:2409195/93‑1 (MQ=255)

                                                            gccagTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAATGCCTGCCAGAAAGt                             >  2:3435343/1‑93 (MQ=255)

                                                            gccagTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAATGCCTGCCAGAAAGt                             <  1:3526745/93‑1 (MQ=255)

                                                            gccagTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAATGCCTGCCAGAAAGt                             <  1:4116332/93‑1 (MQ=255)

                                                            gccagTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAATGCCTGCCAGAAAGt                             >  1:2570327/1‑93 (MQ=255)

                                                              cagTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAATGCCTGCCAGAAAGttt                           <  2:2902638/93‑1 (MQ=255)

                                                               agTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAATGCCTGCCAGAAAGTTTc                          <  1:956021/93‑1 (MQ=255)

                                                                gTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAATGCCTGCCAGAAAGTTTCt                         <  2:571087/93‑1 (MQ=255)

                                                                gTTTAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAATGCCTGCCAGAAAGTTTCt                         >  2:3418693/1‑93 (MQ=255)

                                                                 tttAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAATGCCTGCCAGAAAGTTTCTa                        >  1:140004/1‑93 (MQ=255)

                                                                 tttAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAATGCCTGCCAGAAAGTTTCTa                        >  1:1190613/1‑93 (MQ=255)

                                                                 tttAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAATGCCTGCCAGAAAGTTTCTa                        >  1:1755099/1‑93 (MQ=255)

                                                                  ttAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAATGCCTGCCAGAAAGTTTCTAg                       <  1:3970091/93‑1 (MQ=255)

                                                                   tAATGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAATGCCTGCCAGAAAGTTTCTAgc                      <  1:2540650/93‑1 (MQ=255)

                                                                    aaTGATGTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAATGCCTGCCAGAAAGTTTCTAgcg                     >  2:2429922/1‑93 (MQ=255)

                                                                      tgatgTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAATGCCTGCCAGAAAGTTTCTAGcgcg                   >  2:3543077/1‑93 (MQ=255)

                                                                        atgTTACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAATGCCTGCCAGAAAGTTTCTAGCGCGGt                 >  2:3587686/1‑93 (MQ=255)

                                                                           ttACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAATGCCTGCCAGAAAGTTTCTAGCGCGGTATc              <  2:3335957/93‑1 (MQ=255)

                                                                           ttACGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAATGCCTGCCAGAAAGTTTCTAGCGCGGTATc              >  2:4200535/1‑93 (MQ=255)

                                                                             aCGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAATGCCTGCCAGAAAGTTTCTAGCGCGGTATCaa            <  2:3637140/93‑1 (MQ=255)

                                                                              cGCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAATGCCTGCCAGAAAGTTTCTAGCGCGGTATCAAg           >  1:3427517/1‑93 (MQ=255)

                                                                               gCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAATGCCTGCCAGAAAGTTTCTAGCGCGGTATCAAGa          >  1:3780993/1‑93 (MQ=255)

                                                                               gCATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAATGCCTGCCAGAAAGTTTCTAGCGCGGTATCAAGa          >  1:2486887/1‑93 (MQ=255)

                                                                               cgaTGGGCATTGCCGCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAACGCCTGCCAGAAAGTTTCTAGCGCGGTATCAAGa          <  1:594976/91‑1 (MQ=255)

                                                                                cATGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAATGCCTGCCAGAAAGTTTCTAGCGCGGTATCAAGAc         >  2:378994/1‑93 (MQ=255)

                                                                                  tGGGCATTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAATGCCTGCCAGAAAGTTTCTAGCGCGGTATCAAGACgg       >  1:2425863/1‑93 (MQ=255)

                                                                                       aTTGCCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAATGCCTGCCAGAAAGTTTCTAGCGCGGTATCAAGACGGGTTTg  >  2:507865/1‑93 (MQ=255)

GCTTCAGGCGAGGATTCTGCAGCAACCGCAAAAGTAGAGACGCTAAGCAATCCCGCCAGCGCCAGTTTAATGATGTTACGCATGGGCATTACCTCGCAGAGACAGAAGGTCAGGAACCTGAACATCGCGGCGGTGCAATGCCTGCCAGAAAGTTTCTAGCGCGGTATCAAGACGGGTTTG  >  NC_000913/996085‑996264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`