BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,035,719	C→T	T48M (ACG→ATG)	hyaE →	HyaE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,035,719	0	C	T	99.2%	470.6 / ‑5.2	133	T48M (ACG→ATG)	hyaE	HyaE
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (62/70); total (62/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.74e-01

Only 100 of 142 total aligned reads displayed.

GCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTG  >  NC_000913/1035629‑1035808

  tGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGTCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCa                                                                                       <  1:4673266/93‑1 (MQ=255)

    gACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCaga                                                                                     <  2:3343337/93‑1 (MQ=255)

    gACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCaga                                                                                     <  1:3644327/93‑1 (MQ=255)

     aCGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCagag                                                                                    <  1:645421/93‑1 (MQ=255)

     aCGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCagag                                                                                    <  1:1731101/93‑1 (MQ=255)

       gCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGt                                                                                  <  2:553873/93‑1 (MQ=255)

        ccAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTc                                                                                 <  1:3560687/93‑1 (MQ=255)

          agtcagtAAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAg                                                                               <  2:4084644/93‑1 (MQ=255)

           gtcagtGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGc                                                                              <  1:168217/93‑1 (MQ=255)

            tcagtGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCg                                                                             >  2:4649532/1‑93 (MQ=255)

            tcagtGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACACGAAACGCATGCCAGAGGTCAGCg                                                                             >  2:3781827/1‑93 (MQ=255)

             cagtGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGa                                                                            <  1:977109/93‑1 (MQ=255)

              agtGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAAAGCATGCCAGAGGTCAGCGAt                                                                           >  2:3458192/1‑93 (MQ=255)

                tGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATaa                                                                         <  1:3314421/93‑1 (MQ=255)

                ggAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATaa                                                                         <  2:3236030/92‑1 (MQ=255)

                  aaTCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATc                                                                       >  2:1293755/1‑93 (MQ=255)

                  aaTCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATc                                                                       <  2:3894554/93‑1 (MQ=255)

                  aaTCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATc                                                                       >  2:485721/1‑93 (MQ=255)

                   aTCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATcc                                                                      >  1:1329131/1‑93 (MQ=255)

                   aTCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATcc                                                                      <  1:530995/93‑1 (MQ=255)

                   aTCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATcc                                                                      >  1:4115702/1‑93 (MQ=255)

                    tCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCg                                                                     >  2:2252287/1‑93 (MQ=255)

                     cccGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCgg                                                                    <  2:520026/93‑1 (MQ=255)

                     cccGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCgg                                                                    <  2:4446614/93‑1 (MQ=255)

                        gTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTaa                                                                 >  1:2303230/1‑93 (MQ=255)

                         tCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAAt                                                                >  1:718125/1‑93 (MQ=255)

                          cTTGACGACTGGCTTACGCAAGTGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATg                                                               <  1:2859064/93‑1 (MQ=255)

                          cTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATg                                                               <  1:743895/93‑1 (MQ=255)

                           ttGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGa                                                              <  2:2559394/93‑1 (MQ=255)

                           ttGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGa                                                              >  2:425225/1‑93 (MQ=255)

                            tGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGAt                                                             >  1:4223748/1‑93 (MQ=255)

                             gacgacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTACTGAtt                                                            >  2:241293/1‑93 (MQ=255)

                             gacgacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGAtt                                                            >  2:2100184/1‑93 (MQ=255)

                             gacgacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGAtt                                                            <  1:4243874/93‑1 (MQ=255)

                              acgacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTg                                                           >  1:1910868/1‑93 (MQ=255)

                              acgacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTg                                                           <  1:357641/93‑1 (MQ=255)

                              acgacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTg                                                           <  2:1883280/93‑1 (MQ=255)

                              acgacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTg                                                           >  2:2667375/1‑93 (MQ=255)

                              acgacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTg                                                           <  2:1048208/93‑1 (MQ=255)

                                gacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGc                                                         >  1:3287315/1‑93 (MQ=255)

                                 acTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCg                                                        >  1:3663400/1‑93 (MQ=255)

                                 acTGGCTTACGAAAGCGGCAGGCGGCGTGGAGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGCCAGCGATATTCCGGTAATGATTgggg                                                        >  2:3078584/1‑91 (MQ=255)

                                  cTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGa                                                       >  2:4093224/1‑93 (MQ=255)

                                  cTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGa                                                       <  2:2493445/93‑1 (MQ=255)

                                   tGGCTTACTCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGaa                                                      <  2:1238939/93‑1 (MQ=255)

                                   tGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGaa                                                      <  1:804871/93‑1 (MQ=255)

                                     gCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAAtt                                                    <  1:3354956/93‑1 (MQ=255)

                                       ttACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTAc                                                  >  2:1456158/1‑93 (MQ=255)

                                       ttACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTAc                                                  >  2:3483280/1‑93 (MQ=255)

                                       ttACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTAc                                                  <  2:367438/93‑1 (MQ=255)

                                        tACGCACGCGCCAGGCGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCTAATTACt                                                 <  2:429684/93‑1 (MQ=255)

                                          cGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTgc                                               <  2:624590/93‑1 (MQ=255)

                                            cAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTgcgc                                             <  2:2714813/93‑1 (MQ=255)

                                             aaGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGcgcg                                            >  1:1269991/1‑93 (MQ=255)

                                               gcgcCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAg                                          <  1:4106437/93‑1 (MQ=255)

                                               gcgcCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAg                                          <  1:1683012/93‑1 (MQ=255)

                                                 gcCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGtt                                        <  2:1210510/93‑1 (MQ=255)

                                                  ccAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGttt                                       <  2:2871066/93‑1 (MQ=255)

                                                   cAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTc                                      <  2:3299914/93‑1 (MQ=255)

                                                     gACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTccc                                    >  2:3562248/1‑93 (MQ=255)

                                                     gACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTccc                                    >  2:1485897/1‑93 (MQ=255)

                                                      aCGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCg                                   >  2:1395401/1‑93 (MQ=255)

                                                        ggCGTTGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGAc                                 <  2:1762453/93‑1 (MQ=255)

                                                          cGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACta                               <  1:3133734/93‑1 (MQ=255)

                                                           gtggtgTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACtat                              >  2:2265816/1‑93 (MQ=255)

                                                             ggtgTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATAc                            <  2:1508388/93‑1 (MQ=255)

                                                               tgtTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACAt                          <  2:361177/93‑1 (MQ=255)

                                                               tgtTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACAt                          >  1:675467/1‑93 (MQ=255)

                                                               tgtTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACAt                          >  1:3227378/1‑93 (MQ=255)

                                                               tgtTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACAt                          >  1:1478661/1‑93 (MQ=255)

                                                               tgtTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACAt                          <  1:2847244/93‑1 (MQ=255)

                                                                gtTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATg                         <  1:1102566/93‑1 (MQ=255)

                                                                gtTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATg                         <  2:3186280/93‑1 (MQ=255)

                                                                   attaAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCa                      <  1:2025470/93‑1 (MQ=255)

                                                                   attaAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCa                      <  1:4007078/93‑1 (MQ=255)

                                                                   attaAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCa                      >  2:1110328/1‑93 (MQ=255)

                                                                      aaGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAggg                   >  2:919662/1‑92 (MQ=255)

                                                                      aaGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAggg                   >  1:833586/1‑92 (MQ=255)

                                                                      aaGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGt                   <  1:2209293/93‑1 (MQ=255)

                                                                        gCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTgg                 <  2:3070584/93‑1 (MQ=255)

                                                                        gCAGTGACCCGAAACGCATGACAGAGGGCAGCGGTAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTgg                 >  1:1475517/1‑93 (MQ=255)

                                                                          aGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCg               >  2:2334305/1‑93 (MQ=255)

                                                                           gTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGa              >  1:4172131/1‑93 (MQ=255)

                                                                           gTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGa              >  2:400765/1‑93 (MQ=255)

                                                                           gTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGa              >  2:1800303/1‑93 (MQ=255)

                                                                             gACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGAtt            >  2:3213954/1‑93 (MQ=255)

                                                                             gACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGAtt            <  2:2582814/93‑1 (MQ=255)

                                                                               cccGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGc          <  1:3846477/93‑1 (MQ=255)

                                                                               cccGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGc          >  2:885030/1‑93 (MQ=255)

                                                                               cccGAAACGCAGCCCAGAGGCCACCGATAACCCGTAAAGGATTGGCAAATTACTGCGCGAGTTTCCCGACAATACATGGCAGGTGGAGATTGc          <  2:2040980/93‑1 (MQ=255)

                                                                                ccGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGTGTTTGCt         >  2:2867895/1‑93 (MQ=255)

                                                                                  gAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGAGTGCTGa       >  2:735955/1‑93 (MQ=255)

                                                                                   aaaCGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGAc      <  1:2550705/93‑1 (MQ=255)

                                                                                   aaaCGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGAc      >  1:2381767/1‑93 (MQ=255)

                                                                                    aaCGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGAcc     >  2:978789/1‑93 (MQ=255)

                                                                                     acgcatgcCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCt    >  1:2523050/1‑93 (MQ=255)

                                                                                     acgcatgcCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCt    <  2:3528260/93‑1 (MQ=255)

                                                                                     acgcatgcCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCt    >  1:4444310/1‑93 (MQ=255)

                                                                                       gcatgcCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTg  >  1:4037694/1‑93 (MQ=255)

                                                                                       gcatgcCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTg  <  2:2801195/93‑1 (MQ=255)

GCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTG  >  NC_000913/1035629‑1035808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`