Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,096,810	A→G	noncoding (66/88 nt)	serX ←	tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,096,810	0	A	G	100.0%	163.5 / NA	61	noncoding (66/88 nt)	serX	tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/28);  total (33/28)

ATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGG‑GGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGG  >  NC_000913/1096722‑1096899                                                                                           |                                                                                          aTCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGccc                                                                                        >  2:783092/1‑93 (MQ=255)  tCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGcccc                                                                                       >  2:2179179/1‑93 (MQ=255)  tCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGcccc                                                                                       >  2:3904473/1‑93 (MQ=255)   ccccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGccccc                                                                                      <  1:3839747/93‑1 (MQ=255)   ccccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGccccc                                                                                      <  1:2780614/93‑1 (MQ=11)     ccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGa                                                                                    <  1:2238369/93‑1 (MQ=255)     ccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGa                                                                                    <  1:2605210/93‑1 (MQ=11)     ccccGGGTTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGa                                                                                    <  1:461193/93‑1 (MQ=11)       ccGGTTTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATa                                                                                  <  1:4670998/93‑1 (MQ=14)       ccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGGTTCGAGCCCCCGATa                                                                                  >  2:1083043/1‑93 (MQ=11)       ccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGGTTCGAGCCCCCGATa                                                                                  >  2:3510371/1‑93 (MQ=11)        cGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGGTTCGAGCCCCCGATAc                                                                                 >  2:63311/1‑93 (MQ=11)         ggTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACg                                                                                >  1:2889396/1‑93 (MQ=255)         ggTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACg                                                                                >  2:2529119/1‑93 (MQ=255)          gtgtgtGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGt                                                                               <  1:4527832/93‑1 (MQ=255)           tgtgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGGTTCGAGCCCCCGATACGtt                                                                              >  2:4072192/1‑93 (MQ=14)            gtgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGCTTCGAGCCCCCGATACGTTg                                                                             >  1:2700770/1‑93 (MQ=14)             tgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGGTTCGAGCCCCCGATACGGTGc                                                                            >  2:1537258/1‑93 (MQ=14)              gtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGGTACGTTGcc                                                                           >  2:923537/1‑93 (MQ=14)               tgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTTGAGCCCCCGATACGTTGCCg                                                                          >  1:3038838/1‑93 (MQ=14)               tgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGACCCCGATCCGTTGCCg                                                                          >  2:3295163/1‑93 (MQ=14)                 cAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGta                                                                        >  2:851119/1‑93 (MQ=255)                  aaTATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGCGCCCCCGATACGTTGCCGtat                                                                       >  2:2094669/1‑93 (MQ=14)                  aaTATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGtat                                                                       >  1:26516/1‑93 (MQ=255)                  aaTATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGtat                                                                       <  1:2627546/93‑1 (MQ=255)                  aaTATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCCATACGTTGCCGtat                                                                       >  1:511349/1‑93 (MQ=14)                   atatACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGGTTCGAGGCCCCGATACGTTGCCGtata                                                                      >  2:4325931/1‑93 (MQ=14)                   atatACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGtata                                                                      >  2:2358993/1‑93 (MQ=255)                   atatACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGtata                                                                      >  1:164665/1‑93 (MQ=255)                    tataCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGGTTCGAGCCCCCGATACGTTGCCGTATac                                                                     >  2:4345332/1‑93 (MQ=14)                    tataCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGGCCCCGATAAGTTGCCCTATac                                                                     >  2:2722773/1‑93 (MQ=14)                    tataCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGTATac                                                                     <  1:3638004/93‑1 (MQ=255)                    tataCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGTATac                                                                     >  1:3030161/1‑93 (MQ=255)                    tataCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGCGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGTATac                                                                     >  1:1515269/1‑93 (MQ=14)                     ataCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTCAGGGGG‑GGGACTCGAGGCCCCGATACGTTTCCGtatgcc                                                                    >  2:3143215/1‑90 (MQ=14)                      taCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGGTGCCGTATacac                                                                   >  1:1059185/1‑93 (MQ=14)                       aCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGTATacaca                                                                  <  1:3134708/93‑1 (MQ=255)                        agAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGTATacacac                                                                 <  1:2602377/92‑1 (MQ=11)                         gAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGTATACACACt                                                                <  1:721540/93‑1 (MQ=11)                         gAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGTATACACACt                                                                >  2:1622323/1‑93 (MQ=255)                         gAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGTATACACACt                                                                <  1:176851/93‑1 (MQ=255)                         gAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGTATACACACt                                                                >  2:993932/1‑93 (MQ=255)                         aaaaaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGTATACACACt                                                                <  2:4329827/92‑1 (MQ=11)                          aaaaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGTTTCGAGCCCCCGATACGTTGCCGTATACACACtt                                                               >  1:3516325/1‑93 (MQ=11)                          aaaaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGTATacacacgt                                                               >  2:1938479/1‑91 (MQ=11)                          aaaaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGTATACACACtt                                                               <  1:4314797/93‑1 (MQ=255)                          aaaaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGTATACACACtt                                                               <  2:4661293/93‑1 (MQ=255)                          aaaaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGTATACACACtt                                                               >  2:2363329/1‑93 (MQ=255)                           aaaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGTATACACACttt                                                              >  2:593372/1‑93 (MQ=255)                            aaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTc                                                             >  2:749043/1‑93 (MQ=255)                            aaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTc                                                             >  1:2378245/1‑93 (MQ=255)                             aaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGG‑GGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCa                                                           >  1:1425296/1‑93 (MQ=255)                               aaaGCCCGTGCTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAg                                                          <  1:2919996/93‑1 (MQ=11)                               aaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAg                                                          <  1:3919124/93‑1 (MQ=255)                               aaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAg                                                          <  1:4363631/93‑1 (MQ=255)                               aaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGAGGGATTGGAGGCCCCGATACGTTCACGTATAAACACTTTCCaa                                                          >  1:3459519/1‑92 (MQ=11)                                aaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAgg                                                         >  1:983471/1‑93 (MQ=255)                                 aGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGc                                                        <  1:4654632/93‑1 (MQ=255)                                   cccGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGGTTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGt                                                      >  2:1650302/1‑93 (MQ=11)                                   cccGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGt                                                      <  1:4282952/93‑1 (MQ=255)                                   cccGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGt                                                      >  1:397952/1‑93 (MQ=255)                                    ccGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTg                                                     >  1:824793/1‑93 (MQ=255)                                      gTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGGTTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCt                                                   >  2:3381902/1‑93 (MQ=11)                                      gTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCt                                                   >  2:1567058/1‑93 (MQ=255)                                       tACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGGTTCGAGCCCCCGATACGTTGCCGTATACACACTTTTCAGGCGTGCTc                                                  >  2:1286015/1‑93 (MQ=11)                                       tACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTc                                                  >  2:1383840/1‑93 (MQ=255)                                                                       ggTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTc                  <  1:662640/93‑1 (MQ=255)                                                                       ggTGAGGGGG‑GGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTc                  <  2:1367082/93‑1 (MQ=255)                                                                            ggggg‑gggATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTcc             <  2:3634890/92‑1 (MQ=255)                                                                            ggggg‑gggATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTcc             <  1:2914673/92‑1 (MQ=255)                                                                             gggg‑gggATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCt            >  2:1826898/1‑93 (MQ=255)                                                                               gg‑gggATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGt          <  2:4564773/93‑1 (MQ=255)                                                                                   ggATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCtt       >  2:2731822/1‑93 (MQ=255)                                                                                   ggATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCtt       >  1:2729378/1‑93 (MQ=255)                                                                                    gATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTg      >  1:3679220/1‑93 (MQ=255)                                                                                      ttCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCt    <  2:4262015/93‑1 (MQ=255)                                                                                      ttCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCt    <  1:1604871/93‑1 (MQ=255)                                                                                        cGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTgg  >  2:782752/1‑93 (MQ=255)                                                                                           |                                                                                          ATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGG‑GGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGG  >  NC_000913/1096722‑1096899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑