Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 102,844 A→G Q204Q (CAA→CAG)  ddlB → D‑alanine‑D‑alanine ligase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913102,8440AG99.5% 616.8 / ‑4.8 195Q204Q (CAA→CAG) ddlBD‑alanine‑D‑alanine ligase B
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (100/94);  total (101/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.94e-01

Only 100 of 199 total aligned reads displayed.

TGAAGAAGTATTGATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAACCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTCACAAGA  >  NC_000913/102754‑102933
                                                                                          |                                                                                         
 ggagaagTATTGATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCg                                                                                        >  2:3732904/3‑93 (MQ=255)
  aagaagTATTGATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGt                                                                                       >  2:2058151/1‑93 (MQ=255)
  aagaagTATTGATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGt                                                                                       <  1:3768162/93‑1 (MQ=255)
    gaagTATTGATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCTGTcc                                                                                     >  1:584852/1‑93 (MQ=255)
     aagTATTGATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCg                                                                                    <  1:3551921/93‑1 (MQ=255)
     aagTATTGATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCg                                                                                    >  2:3627889/1‑93 (MQ=255)
     aagTATTGATTAAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCg                                                                                    <  1:3215317/93‑1 (MQ=255)
      agTATTGATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGATTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCgg                                                                                   >  2:3824818/1‑93 (MQ=255)
       gTATTGATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGa                                                                                  >  1:2725173/1‑93 (MQ=255)
        tATTGATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATATGTATTCAGCCGTCCGCaa                                                                                 >  1:2455842/1‑93 (MQ=255)
         attgattgAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAAc                                                                                <  2:3996652/93‑1 (MQ=255)
            gattgaAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCtt                                                                             <  2:4370949/93‑1 (MQ=255)
            gattgaAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCtt                                                                             >  1:4647407/1‑93 (MQ=255)
             attgaAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTc                                                                            >  1:4039186/1‑93 (MQ=255)
             attgaAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTc                                                                            <  1:454897/93‑1 (MQ=255)
              ttgaAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCt                                                                           >  2:88562/1‑93 (MQ=255)
               tgaAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTa                                                                          <  2:1708495/93‑1 (MQ=255)
               tgaAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTa                                                                          <  1:2777705/93‑1 (MQ=255)
                gaAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTAt                                                                         <  2:1844054/93‑1 (MQ=255)
                gaAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTAt                                                                         <  1:2609560/93‑1 (MQ=255)
                   aaaTGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATCCGTATTCAGCCGTCCGGAACCTTCTATGAt                                                                      <  1:2892814/93‑1 (MQ=255)
                   aaaTGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGAt                                                                      >  1:1460177/1‑93 (MQ=255)
                   aaaTGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGAt                                                                      <  2:702962/93‑1 (MQ=255)
                    aaTGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGAtt                                                                     >  2:197140/1‑93 (MQ=255)
                    aaTGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGAtt                                                                     <  1:3231063/93‑1 (MQ=255)
                     aTGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGTGGTCGGGAACCTTCTATGtttt                                                                    >  1:1030067/1‑92 (MQ=255)
                     aTGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTa                                                                    <  2:3271532/93‑1 (MQ=255)
                     aTGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTa                                                                    <  1:536682/93‑1 (MQ=255)
                     aTGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTAACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTa                                                                    <  1:2513375/93‑1 (MQ=255)
                     aTGGCTAAGTGGGCCGGAGGTCACGGTTGCGATACTCCGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGTTTa                                                                    >  1:1157351/1‑93 (MQ=255)
                     aTGGCTAAGGGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTa                                                                    <  1:898519/93‑1 (MQ=255)
                       ggCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATg                                                                  <  2:3839182/93‑1 (MQ=255)
                        gCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGa                                                                 >  1:3934104/1‑93 (MQ=255)
                         cTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAg                                                                >  1:3315007/1‑93 (MQ=255)
                          tAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAgg                                                               >  2:1352408/1‑93 (MQ=255)
                           aaGTGGTCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGc                                                              <  1:781368/93‑1 (MQ=255)
                           aaGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGc                                                              >  1:2414054/1‑93 (MQ=255)
                           aaGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGc                                                              >  2:4553915/1‑93 (MQ=255)
                           aaGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGc                                                              >  2:4394977/1‑93 (MQ=255)
                              tGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATCCGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGaa                                                           <  2:2982930/93‑1 (MQ=255)
                               gggCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAg                                                          >  1:4105973/1‑93 (MQ=255)
                               gggCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAg                                                          <  1:1114445/93‑1 (MQ=255)
                                ggCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGt                                                         >  1:3413329/1‑93 (MQ=255)
                                 gCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTa                                                        <  1:1833157/93‑1 (MQ=255)
                                 gCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTa                                                        <  1:933826/93‑1 (MQ=255)
                                 gCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTa                                                        >  2:520021/1‑93 (MQ=255)
                                  ccGGAGTTCACTGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTAt                                                       <  1:2291857/93‑1 (MQ=255)
                                  ccGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTAt                                                       <  1:1475576/93‑1 (MQ=255)
                                    ggAGTTCACGGTTGCGATACTCGGTGAAAAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTAtct                                                     <  2:1991203/93‑1 (MQ=255)
                                     gAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGGTTATGAGGCGAAGtctctc                                                    >  1:3611820/1‑93 (MQ=255)
                                     gAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTAtctc                                                    <  1:3590683/93‑1 (MQ=255)
                                       gTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTAtctctc                                                  <  2:3800830/93‑1 (MQ=255)
                                        ttCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATctctct                                                 >  1:2504197/1‑93 (MQ=255)
                                        ttCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATctctct                                                 >  2:3622100/1‑93 (MQ=255)
                                        ttCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATctctct                                                 <  1:2387211/93‑1 (MQ=255)
                                         tCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCtg                                                >  2:252359/1‑93 (MQ=255)
                                          cACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCtga                                               <  2:1246611/93‑1 (MQ=255)
                                          cACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATACAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCtga                                               <  1:4396420/93‑1 (MQ=255)
                                           aCGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCtgat                                              >  1:1011677/1‑93 (MQ=255)
                                              gTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCtgatgaa                                           >  1:1649643/1‑92 (MQ=255)
                                                 gCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGaca                                        <  2:433098/93‑1 (MQ=255)
                                                   gATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGAcaca                                      >  1:2831545/1‑93 (MQ=255)
                                                    agaCTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAg                                     <  2:4023626/91‑1 (MQ=255)
                                                     tACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGt                                    >  2:2736050/1‑93 (MQ=255)
                                                     tACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGt                                    >  2:554893/1‑93 (MQ=255)
                                                      aCTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTa                                   <  2:2232707/93‑1 (MQ=255)
                                                      aCTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTa                                   <  2:2500924/93‑1 (MQ=255)
                                                       cTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTAt                                  <  1:1641999/93‑1 (MQ=255)
                                                       cTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTAt                                  <  1:2484849/93‑1 (MQ=255)
                                                          ggTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTc                               <  2:336612/93‑1 (MQ=255)
                                                           gTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCt                              <  2:1158275/93‑1 (MQ=255)
                                                              aagaaATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGcc                           >  2:2165439/1‑93 (MQ=255)
                                                               agaaATTTTACCGTCAATACGTATTCAACCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGccc                          >  2:1454879/1‑93 (MQ=255)
                                                                    ttttACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAg                     >  2:2672719/1‑93 (MQ=255)
                                                                    ttttACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAg                     <  1:1973982/93‑1 (MQ=255)
                                                                     tttACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAgg                    <  1:436000/93‑1 (MQ=255)
                                                                       tACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTc                  >  1:2544958/1‑93 (MQ=255)
                                                                       tACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTc                  <  1:621167/93‑1 (MQ=255)
                                                                        aCCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCt                 >  2:27943/1‑93 (MQ=255)
                                                                        aCCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCt                 <  1:3801292/93‑1 (MQ=255)
                                                                        aCCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCt                 >  1:4271279/1‑93 (MQ=255)
                                                                          cGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTgg               <  2:13762/93‑1 (MQ=255)
                                                                           gTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGa              >  1:2624486/1‑93 (MQ=255)
                                                                            tCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGaa             >  2:1930956/1‑93 (MQ=255)
                                                                            tCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGaa             >  1:3756844/1‑93 (MQ=255)
                                                                              aaTACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCACGGCAGGTCTGGAAgg           >  2:3158704/1‑92 (MQ=255)
                                                                               aTACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCg          <  1:1697423/93‑1 (MQ=255)
                                                                               aTACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCg          <  1:3554111/93‑1 (MQ=255)
                                                                               aTACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCg          >  1:2915671/1‑93 (MQ=255)
                                                                                tACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGt         >  2:4471350/1‑93 (MQ=255)
                                                                                tACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGt         >  2:1393431/1‑93 (MQ=255)
                                                                                tACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGt         >  1:258432/1‑93 (MQ=255)
                                                                                 aCGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTc        >  1:466132/1‑93 (MQ=255)
                                                                                  cGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTca       >  2:4179951/1‑93 (MQ=255)
                                                                                  cGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTca       >  2:1216848/1‑93 (MQ=255)
                                                                                   gTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTcac      >  1:3644917/1‑93 (MQ=255)
                                                                                     aTTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTCACaa    <  1:1534957/93‑1 (MQ=255)
                                                                                     aTTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTCACaa    >  1:675313/1‑93 (MQ=255)
                                                                                     aTTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTCACaa    >  2:622478/1‑93 (MQ=255)
                                                                                       tCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTCACAaga  <  1:2970422/93‑1 (MQ=255)
                                                                                          |                                                                                         
TGAAGAAGTATTGATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAACCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTCACAAGA  >  NC_000913/102754‑102933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑