BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,167,731	T→C	T109A (ACC→GCC)	ycfQ ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,167,731	0	T	C	99.1%	363.8 / ‑4.6	116	T109A (ACC→GCC)	ycfQ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (37/78); total (37/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.58e-01

Only 100 of 117 total aligned reads displayed.

TTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGTAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCTGTGCTTCATGCTTAGC  >  NC_000913/1167642‑1167818

tttCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCaaa                                                                                      <  2:1857670/93‑1 (MQ=255)

tttCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCaaa                                                                                      <  1:3991525/93‑1 (MQ=255)

tttCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCaaa                                                                                      <  2:3403553/93‑1 (MQ=255)

 ttCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAg                                                                                     <  1:697964/93‑1 (MQ=255)

  tCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGc                                                                                    <  1:2909181/93‑1 (MQ=255)

   cAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCa                                                                                   <  1:3669543/93‑1 (MQ=255)

   cAACGTATTGGCGATAGCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCa                                                                                   <  1:3901369/93‑1 (MQ=255)

    aaCGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAg                                                                                  <  1:2343058/93‑1 (MQ=255)

     aCGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGTAAAGCAGt                                                                                 <  1:1023992/93‑1 (MQ=255)

      cGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGtt                                                                                <  2:222957/93‑1 (MQ=255)

      cGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGtt                                                                                <  2:1350289/93‑1 (MQ=255)

       gTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTg                                                                               <  1:1752607/93‑1 (MQ=255)

        tATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTgg                                                                              <  2:3580800/93‑1 (MQ=255)

        tATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTgg                                                                              <  1:1193553/93‑1 (MQ=255)

         aTTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGc                                                                             <  2:3273517/93‑1 (MQ=255)

             gCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATg                                                                         <  2:48919/93‑1 (MQ=255)

                atatCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGgca                                                                      >  1:1006586/1‑93 (MQ=255)

                 tatCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGgcag                                                                     >  1:3424178/1‑93 (MQ=255)

                  atCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGgcagc                                                                    >  1:3667782/1‑93 (MQ=255)

                  atCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGgcagc                                                                    <  2:1551103/93‑1 (MQ=255)

                   tctcCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGgcagca                                                                   >  1:1434487/1‑93 (MQ=255)

                   tctcCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGgcagca                                                                   >  2:4021850/1‑93 (MQ=255)

                   tctcCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGgcagca                                                                   >  1:1655774/1‑93 (MQ=255)

                   tctcCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGgcagca                                                                   <  2:1809442/93‑1 (MQ=255)

                    ctcCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCaa                                                                  <  1:907737/93‑1 (MQ=255)

                     tcCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCaaa                                                                 >  1:4253773/1‑93 (MQ=255)

                     tcCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCaaa                                                                 >  1:4620116/1‑93 (MQ=255)

                     tcCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCaaa                                                                 >  1:2688300/1‑93 (MQ=255)

                     tcCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCaaa                                                                 >  2:3237754/1‑93 (MQ=255)

                      ccTGAATCTGGGGAGAGGGTGGTGNAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCaaaa                                                                <  2:704113/93‑1 (MQ=255)

                      ccTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCaaaa                                                                <  1:1249478/93‑1 (MQ=255)

                       cTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAAt                                                               <  2:3020910/93‑1 (MQ=255)

                       cTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAAt                                                               >  1:2020089/1‑93 (MQ=255)

                        tGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATa                                                              >  2:4414373/1‑93 (MQ=255)

                        tGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATa                                                              <  2:4050983/93‑1 (MQ=255)

                         gAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAg                                                             <  1:3490357/93‑1 (MQ=255)

                           aTCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTc                                                           >  2:240035/1‑93 (MQ=255)

                           aTCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTc                                                           <  1:1026323/93‑1 (MQ=255)

                           aTCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTc                                                           >  2:726581/1‑93 (MQ=255)

                            tCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTca                                                          >  2:2192748/1‑93 (MQ=255)

                               ggggAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTcagcc                                                       <  1:4303672/93‑1 (MQ=255)

                                gggAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTcagcca                                                      <  1:1585074/93‑1 (MQ=255)

                                gggAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTcagcca                                                      <  1:1619909/93‑1 (MQ=255)

                                 ggAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTcagccag                                                     <  2:2830202/93‑1 (MQ=255)

                                  gagaGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTcagccagc                                                    <  1:133127/93‑1 (MQ=255)

                                  gagaGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTcagccagc                                                    <  1:3503935/93‑1 (MQ=255)

                                    gagGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAgcgc                                                  <  1:4402496/93‑1 (MQ=255)

                                        gtggtgCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGAc                                              <  1:1069634/93‑1 (MQ=255)

                                        gtggtgCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGAc                                              >  1:682413/1‑93 (MQ=255)

                                         tggtgCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACt                                             >  2:1560931/1‑93 (MQ=255)

                                          ggtgCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTc                                            <  2:889427/93‑1 (MQ=255)

                                           gtgCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTcc                                           <  2:2425301/93‑1 (MQ=255)

                                           gtgCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTcc                                           <  1:3709957/93‑1 (MQ=255)

                                            tgtaGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCa                                          <  2:848861/90‑1 (MQ=255)

                                             gCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCAc                                         <  2:769771/93‑1 (MQ=255)

                                             gCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCAc                                         <  1:1588831/93‑1 (MQ=255)

                                              cAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACg                                        <  2:1851638/93‑1 (MQ=255)

                                               aGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGTAGACTCCACGc                                       >  1:349680/1‑93 (MQ=255)

                                                 ttgttgATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCtt                                     <  1:2311674/93‑1 (MQ=255)

                                                  tgttgATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCttt                                    <  2:2206169/93‑1 (MQ=255)

                                                   gttgATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCtttt                                   <  2:3849815/93‑1 (MQ=255)

                                                    ttgATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTtc                                  <  2:2584158/93‑1 (MQ=255)

                                                     tgATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTtct                                 <  1:2866366/93‑1 (MQ=255)

                                                       aTCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTctct                               <  1:1791847/93‑1 (MQ=255)

                                                         cATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTc                             <  2:359622/93‑1 (MQ=255)

                                                         cATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTc                             <  1:587848/93‑1 (MQ=255)

                                                          aTGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTca                            >  1:4508464/1‑93 (MQ=255)

                                                          aTGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTca                            >  2:230782/1‑93 (MQ=255)

                                                           tGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTcac                           <  2:492067/93‑1 (MQ=255)

                                                           tGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTcac                           >  2:3412415/1‑93 (MQ=255)

                                                            gAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTcaca                          >  2:520372/1‑93 (MQ=255)

                                                               gcagcCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACaaaa                       <  1:2777735/93‑1 (MQ=255)

                                                                cagcCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAAc                      <  2:3017331/93‑1 (MQ=255)

                                                                 agcCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATATTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACa                     <  2:3235936/93‑1 (MQ=255)

                                                                 agcCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCATCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACa                     <  1:2392720/93‑1 (MQ=255)

                                                                 agcCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACa                     <  1:1008878/93‑1 (MQ=255)

                                                                 agcCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACa                     <  1:3491272/93‑1 (MQ=255)

                                                                  gcCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAg                    >  1:357238/1‑93 (MQ=255)

                                                                   ccTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGc                   >  1:3371264/1‑93 (MQ=255)

                                                                    cTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCt                  <  1:3167984/93‑1 (MQ=255)

                                                                    cTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCt                  >  1:3133276/1‑93 (MQ=255)

                                                                     tgtcGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCtg                 <  2:162354/90‑1 (MQ=255)

                                                                     tGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCtg                 >  2:628391/1‑93 (MQ=255)

                                                                     tGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCtg                 >  1:2374810/1‑93 (MQ=255)

                                                                       ccGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCtgtg               >  2:3071826/1‑93 (MQ=255)

                                                                        cGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGAATCCACGCTTTTCTCTTCACAAAACAGCTGTGc              >  1:4255276/1‑93 (MQ=255)

                                                                         ggggTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCtgagtt             >  2:3425138/1‑89 (MQ=255)

                                                                          gggTGTCTTTGCTGGCAAAGCAGTTGGCTATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCTGTGCtt            <  1:452807/93‑1 (MQ=255)

                                                                            gtgtCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCTGTGCTTCa          <  2:277087/93‑1 (MQ=255)

                                                                             tgtCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCTGTGCTTCAt         <  1:659417/93‑1 (MQ=255)

                                                                             tgtCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCTGTGCTTCAt         <  2:2568610/93‑1 (MQ=255)

                                                                             tgtCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCTGTGCTTCAt         <  2:1700900/93‑1 (MQ=255)

                                                                               tCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCTGTGCTTCATGc       >  2:1474160/1‑93 (MQ=255)

                                                                               tCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCTGTGCTTCATGc       <  1:1436880/93‑1 (MQ=255)

                                                                               tCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCTGTGCTTCATGc       >  2:926060/1‑93 (MQ=255)

                                                                                cTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCTGTGCTTCATGCt      <  1:2216026/93‑1 (MQ=255)

                                                                                cTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCTGTGCTTCATGCt      <  2:908560/93‑1 (MQ=255)

                                                                                 tttGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCTGTGCTTCATGCtt     <  2:2516057/93‑1 (MQ=255)

                                                                                 tttGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCTGTGCTTCATGCtt     <  1:1227387/93‑1 (MQ=255)

                                                                                    gCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCTGTGCTTCATGCTTAGc  <  1:1081012/93‑1 (MQ=255)

TTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGTAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCTGTGCTTCATGCTTAGC  >  NC_000913/1167642‑1167818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`