BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,178,755	C→T	R5W (CGG→TGG)	cobB →	putative nicotinic acid mononucleotide:5,6‑dimethylbenzimidazole (DMB) phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,178,755	0	C	T	100.0%	442.5 / NA	123	R5W (CGG→TGG)	cobB	putative nicotinic acid mononucleotide:5,6‑dimethylbenzimidazole (DMB) phosphoribosyltransferase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (50/73); total (50/73)

Only 100 of 128 total aligned reads displayed.

AACGCGCGCGCCACGGTGATGCGGGAGGAATGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTATTTTTTTCAGAGATAAAGTGGTG  >  NC_000913/1178663‑1178844

aaCGCGCGCGCCACGGTGATGCGGGAGGAATGCGTGGTGCGGCCTTCCTACATCTAACCGATTAACCAACAGAGGTTGCTATGCTGTCGCGTc                                                                                           >  1:1184234/1‑93 (MQ=255)

  cgcgcgcgCCACGGTGATGCGGGAGGAATGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTgg                                                                                         <  2:4109122/93‑1 (MQ=255)

  cgcgcgcgCCACGGTGATGCGGGAGGAATGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTgg                                                                                         <  1:1387682/93‑1 (MQ=255)

   gcgcgcgcCACGGTGATGCGGGAGGAATGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTggg                                                                                        <  1:4164635/93‑1 (MQ=255)

   gcgcgcgcCACGGTGATGCGGGAGGAATGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTggg                                                                                        <  2:2583976/93‑1 (MQ=255)

     gcgcgcCACGGTGATGCGGGAGGAATGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGt                                                                                      <  2:1766503/93‑1 (MQ=255)

        cgcCACGGTGATGCGGGAGGAATGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCAt                                                                                   >  1:3030193/1‑93 (MQ=255)

        cgcCACGGTGATGCGGGAGGAATGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCAt                                                                                   <  2:943803/93‑1 (MQ=255)

         gcCACGGTGATGCGGGAGGAATGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATc                                                                                  <  2:1844431/93‑1 (MQ=255)

         gcCACGGTGATGCGGGAGGAATGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATc                                                                                  <  1:2730197/93‑1 (MQ=255)

            aCGGTGATGCGGGAGGAATGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGt                                                                               <  2:1879114/93‑1 (MQ=255)

             cGGTGATGCGGGAGGAATGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGtt                                                                              <  1:2566611/93‑1 (MQ=255)

                tGATGCGGGAGGAATGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAg                                                                           <  1:3474829/93‑1 (MQ=255)

                 gATGCGGGAGGAATGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGt                                                                          >  1:2285606/1‑93 (MQ=255)

                       ggaggaATGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGtttt                                                                    <  1:130351/93‑1 (MQ=255)

                       ggaggaATGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGtttt                                                                    >  2:241877/1‑93 (MQ=255)

                        gaggaATGCGTGGTGCCGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTc                                                                   <  1:4394699/93‑1 (MQ=255)

                         aggaATGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCg                                                                  <  2:996612/93‑1 (MQ=255)

                          ggaATGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGt                                                                 >  1:461833/1‑93 (MQ=255)

                           gaATGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTa                                                                >  1:2114245/1‑93 (MQ=255)

                           gaATGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTa                                                                >  2:139640/1‑93 (MQ=255)

                            aaTGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTaa                                                               <  1:3147572/93‑1 (MQ=255)

                             aTGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTTGTaaa                                                              >  1:1021829/1‑93 (MQ=255)

                              tGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTaaaa                                                             <  2:4032208/93‑1 (MQ=255)

                              tGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTaaaa                                                             >  2:2788573/1‑93 (MQ=255)

                               gCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTaaaaa                                                            <  2:1329476/93‑1 (MQ=255)

                               gCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTaaaaa                                                            <  1:4042231/93‑1 (MQ=255)

                               gCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTaaaaa                                                            <  1:3821280/93‑1 (MQ=255)

                                 gtggtgCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATa                                                          <  2:609236/93‑1 (MQ=255)

                                 gtggtgCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATa                                                          <  1:974445/93‑1 (MQ=255)

                                  tggtgCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATaa                                                         >  1:4222955/1‑93 (MQ=255)

                                   ggtgCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATaaa                                                        <  1:3660874/93‑1 (MQ=255)

                                   ggtgCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATaaa                                                        <  1:3625054/93‑1 (MQ=255)

                                   ggtgCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATaaa                                                        <  2:4666919/93‑1 (MQ=255)

                                   ggtgCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATaaa                                                        >  2:4687895/1‑93 (MQ=255)

                                    gtgCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAAc                                                       <  1:2004397/93‑1 (MQ=255)

                                    gtgCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAAc                                                       <  2:2518420/93‑1 (MQ=255)

                                    gtgCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAAc                                                       <  2:3640791/93‑1 (MQ=255)

                                    gtgCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAAc                                                       <  1:92069/93‑1 (MQ=255)

                                      gCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAAcgc                                                     <  2:2218101/93‑1 (MQ=255)

                                           cTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACgccgccg                                                >  1:3564193/1‑92 (MQ=255)

                                            ttCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTg                                               >  2:1948277/1‑93 (MQ=255)

                                            ttCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTg                                               <  2:1337030/93‑1 (MQ=255)

                                              ccTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTgcg                                             >  2:1759579/1‑93 (MQ=255)

                                               cTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTgcgc                                            <  1:1007345/93‑1 (MQ=255)

                                                 aCATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGa                                          <  1:2839417/93‑1 (MQ=255)

                                                 aCATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGa                                          <  1:1397376/93‑1 (MQ=255)

                                                   aTCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGc                                        >  2:3765166/1‑93 (MQ=255)

                                                   aTCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGc                                        >  1:1881845/1‑93 (MQ=255)

                                                    tCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCg                                       >  1:577931/1‑93 (MQ=255)

                                                      tAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGtt                                     >  2:1485985/1‑93 (MQ=255)

                                                      tAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGtt                                     <  2:940489/93‑1 (MQ=255)

                                                       aaCCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGttt                                    <  1:2798668/93‑1 (MQ=255)

                                                       aaCCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGCTAAGTCGGTTTCGTAAAAATAAACCCCCCCTGCGCGAGCGttt                                    >  1:4157997/1‑93 (MQ=255)

                                                        aCCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTg                                   <  2:1029018/93‑1 (MQ=255)

                                                         ccGATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGc                                  <  2:4642098/93‑1 (MQ=255)

                                                           gATTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGt                                >  1:2967311/1‑93 (MQ=255)

                                                            aTTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTc                               >  1:4420653/1‑93 (MQ=255)

                                                            aTTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTc                               >  1:3806362/1‑93 (MQ=255)

                                                            aTTAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTc                               >  2:286261/1‑93 (MQ=255)

                                                             ttAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCa                              >  1:3543968/1‑93 (MQ=255)

                                                             ttAAACAACAGAGGTGGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCa                              <  1:2281184/93‑1 (MQ=255)

                                                              tAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAg                             <  1:2653084/93‑1 (MQ=255)

                                                              tAAACAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAg                             >  2:3056477/1‑93 (MQ=255)

                                                               aaaCAACAGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGc                            >  1:2150169/1‑93 (MQ=255)

                                                                 acaacaGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGt                          <  2:3093968/93‑1 (MQ=255)

                                                                  caacaGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTa                         >  2:789835/1‑93 (MQ=255)

                                                                   aacaGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTAt                        >  2:3639959/1‑93 (MQ=255)

                                                                    acaGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTAtt                       >  1:1752708/1‑93 (MQ=255)

                                                                    acaGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTAtt                       >  2:2017065/1‑93 (MQ=255)

                                                                    acaGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTAtt                       <  2:1658102/93‑1 (MQ=255)

                                                                    acaGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTAtt                       >  1:3014663/1‑93 (MQ=255)

                                                                    acaGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTAtt                       <  2:2924366/93‑1 (MQ=255)

                                                                     caGAGGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTAttt                      >  1:3867702/1‑93 (MQ=255)

                                                                      agagGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTAtttt                     <  1:4648142/93‑1 (MQ=255)

                                                                      agagGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTAtttt                     <  1:510905/93‑1 (MQ=255)

                                                                       gatgTTGCTATTCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTAttttt                    <  2:2447764/90‑1 (MQ=255)

                                                                       gagGTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGCGCGTTTGCGTCAGCGTCttttt                    >  1:3289612/1‑93 (MQ=255)

                                                                          gTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTATTTTTTTc                 <  2:4675379/93‑1 (MQ=255)

                                                                          gTTGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTATTTTTTTc                 <  2:4322880/93‑1 (MQ=255)

                                                                           ttGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTATTTTTTTCa                <  2:3131253/93‑1 (MQ=255)

                                                                           ttGCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTATTTTTTTCa                <  2:4117956/93‑1 (MQ=255)

                                                                             tcTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTATTTTTTTCaga              <  2:690858/92‑1 (MQ=255)

                                                                             gCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTATTTTTTTCaga              >  2:3145305/1‑93 (MQ=255)

                                                                             gCTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTATTTTTTTCaga              <  2:1751981/93‑1 (MQ=255)

                                                                              cTATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTATTTTTTTCagag             <  2:4441329/93‑1 (MQ=255)

                                                                               tATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTATTTTTTTCAgaga            >  1:2947398/1‑93 (MQ=255)

                                                                               tATGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTATTTTTTTCAgaga            >  2:4469058/1‑93 (MQ=255)

                                                                                aTGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTATTTTTTTCAGAGAt           <  1:120799/93‑1 (MQ=255)

                                                                                 tGCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTATTTTTTTCAGAGATa          <  1:3477948/93‑1 (MQ=255)

                                                                                  gCTGTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTATTTTTTTCAGAGATaa         >  2:395988/1‑93 (MQ=255)

                                                                                   cTGTCGGGTTCGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAACCGCCGCCTGCGCGAGCGGTTGCGTCAGCGTATTTTTTTCAGAGATaaa        <  1:3682346/93‑1 (MQ=255)

                                                                                     gTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTATTTTTTTCAGAGATAAAgt      <  1:1382249/93‑1 (MQ=255)

                                                                                     gTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTATTTTTTTCAGAGATAAAgt      <  2:2511516/93‑1 (MQ=255)

                                                                                     gTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTATTTTTTTCAGAGATAAAgt      <  1:2233410/93‑1 (MQ=255)

                                                                                     gTCGCGTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTATTTTTTTCAGAGATAAAgg      >  1:516069/1‑92 (MQ=255)

                                                                                       cgcgTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTATTTTTTTCAGAGATAAAgtgg    <  1:1911037/93‑1 (MQ=255)

                                                                                       cgcgTTGGGGTCATCGGTTAAGTCGTTTTAGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTATTTTTTTCAGAGATAAAgtgg    <  2:2612971/93‑1 (MQ=255)

                                                                                        gcgTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTATTTTTTTCAGAGATAAAgtggt   <  2:717312/93‑1 (MQ=255)

                                                                                        gcgTTGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTATTTTTTTCAGAGATAAAgtggt   <  1:2977680/93‑1 (MQ=255)

AACGCGCGCGCCACGGTGATGCGGGAGGAATGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTATTTTTTTCAGAGATAAAGTGGTG  >  NC_000913/1178663‑1178844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`