BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,184,484	A→G	F112L (TTT→CTT)	potA ←	ATP‑binding component of spermidine/putrescine transport

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,184,484	0	A	G	100.0%	458.9 / NA	148	F112L (TTT→CTT)	potA	ATP‑binding component of spermidine/putrescine transport
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (71/77); total (71/77)

Only 100 of 151 total aligned reads displayed.

GAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGG  >  NC_000913/1184394‑1184573

gAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAggg                                                                                         >  1:1064055/1‑93 (MQ=255)

 aGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGc                                                                                        >  1:88933/1‑93 (MQ=255)

  gCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGcc                                                                                       >  2:2799763/1‑93 (MQ=255)

  gCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGcc                                                                                       >  1:3903526/1‑93 (MQ=255)

  gCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGGTTTTTGCATGCGCAACCCAAGGGcc                                                                                       >  1:1386887/1‑93 (MQ=255)

    gAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGccgc                                                                                     >  1:3900615/1‑91 (MQ=255)

        gTTTCCAACGGCACCAGCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCAttt                                                                                 <  1:356216/93‑1 (MQ=255)

          ttCCAGCTCCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTc                                                                               <  2:4270165/93‑1 (MQ=255)

             cAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGaa                                                                            <  2:1119520/93‑1 (MQ=255)

              aaCTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAac                                                                           >  1:3187567/1‑93 (MQ=255)

              aaCTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAac                                                                           >  1:182332/1‑93 (MQ=255)

              aaCTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAac                                                                           >  1:1899220/1‑93 (MQ=255)

              aaCGGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAac                                                                           <  1:1842896/93‑1 (MQ=255)

               aCTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAaca                                                                          >  2:1449846/1‑93 (MQ=255)

               aCTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAaca                                                                          <  1:2663988/93‑1 (MQ=255)

               aCTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAaca                                                                          <  1:915347/93‑1 (MQ=255)

               aCTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAaca                                                                          <  2:476904/93‑1 (MQ=255)

               aCTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGGTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAaca                                                                          >  2:3872000/1‑93 (MQ=255)

                  gCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACgg                                                                       <  2:3262922/93‑1 (MQ=255)

                  gCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACgg                                                                       <  2:400551/93‑1 (MQ=255)

                    aCCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTc                                                                     >  1:2250463/1‑93 (MQ=255)

                    aCCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTc                                                                     >  2:2103403/1‑93 (MQ=255)

                     acATCCGCATGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCa                                                                    <  1:274110/92‑1 (MQ=255)

                      cATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCAt                                                                   <  1:3847681/93‑1 (MQ=255)

                       aTCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCAtg                                                                  <  1:2499452/93‑1 (MQ=255)

                       aTCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCAtg                                                                  >  1:2828504/1‑93 (MQ=255)

                       aTCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCAtg                                                                  <  1:3084145/93‑1 (MQ=255)

                        tCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCAtgt                                                                 <  2:1237288/93‑1 (MQ=255)

                        tCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCAtgt                                                                 <  2:2237311/93‑1 (MQ=255)

                        tCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCAtgt                                                                 >  2:2597183/1‑93 (MQ=255)

                        tCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGATTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCAtgt                                                                 >  1:627373/1‑93 (MQ=255)

                          cGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTgg                                                               >  2:3353655/1‑93 (MQ=255)

                          cGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTgg                                                               >  2:2122055/1‑93 (MQ=255)

                          cGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTgg                                                               <  1:373219/93‑1 (MQ=255)

                           gCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTggg                                                              <  1:2309605/93‑1 (MQ=255)

                            cAGTGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTgggg                                                             <  2:3024004/93‑1 (MQ=255)

                             aGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTggggg                                                            >  2:4320027/1‑93 (MQ=255)

                              gggCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGa                                                           >  1:2624909/1‑93 (MQ=255)

                              gggCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGa                                                           >  1:4283586/1‑93 (MQ=255)

                               ggCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGaa                                                          >  2:2359899/1‑93 (MQ=255)

                                 cTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGaaaa                                                        <  1:1722084/93‑1 (MQ=255)

                                 cTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGaaaa                                                        <  1:1742051/93‑1 (MQ=255)

                                  ttCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAg                                                       >  2:1251632/1‑93 (MQ=255)

                                   tCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAgg                                                      >  1:3316048/1‑92 (MQ=255)

                                   tCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGt                                                      <  2:4240501/93‑1 (MQ=255)

                                     cATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGc                                                    <  2:4240601/93‑1 (MQ=255)

                                      aTCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGCACAGTGCg                                                   >  1:803546/1‑93 (MQ=255)

                                        cACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTa                                                 <  1:2743045/93‑1 (MQ=255)

                                        cACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTa                                                 >  2:3666845/1‑93 (MQ=255)

                                          cgcgGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGc                                               <  1:254083/93‑1 (MQ=255)

                                          cgcgGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGc                                               <  2:4151988/93‑1 (MQ=255)

                                          cgcgGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGc                                               <  1:2934718/93‑1 (MQ=255)

                                          cgcgGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGc                                               <  1:990102/93‑1 (MQ=255)

                                           gcgGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCt                                              <  2:156938/93‑1 (MQ=255)

                                            cgGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCtt                                             <  1:1583017/93‑1 (MQ=255)

                                             ggggCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCttt                                            >  1:4673014/1‑93 (MQ=255)

                                               ggCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCATCCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTgg                                          <  2:1393673/93‑1 (MQ=255)

                                               ggCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGACCACGGTCATGTGGGGGAAAAGTGCGTAGCTTTga                                          >  1:2124141/1‑92 (MQ=255)

                                                   tAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGaaaa                                      >  1:3006347/1‑93 (MQ=255)

                                                   tAATTTCAGCAGCGGGGGGTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAATAGTGCGTAGGTTTGGaaaa                                      >  2:3467097/1‑93 (MQ=255)

                                                     aTTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACa                                    >  1:3947651/1‑93 (MQ=255)

                                                     aTTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACa                                    <  1:208624/93‑1 (MQ=255)

                                                     aTTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACa                                    >  1:3371035/1‑93 (MQ=255)

                                                     aTTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACa                                    >  1:2739506/1‑93 (MQ=255)

                                                      tttCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAg                                   <  2:2133262/93‑1 (MQ=255)

                                                      tttCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAg                                   <  2:2276210/93‑1 (MQ=255)

                                                        tCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAgtg                                 <  2:3174152/93‑1 (MQ=255)

                                                          agcagcGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGtt                               <  1:952545/93‑1 (MQ=255)

                                                            cagcGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTca                             <  1:4617716/93‑1 (MQ=255)

                                                              gcGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTcaca                           <  1:2181290/93‑1 (MQ=255)

                                                              gcGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTcaca                           <  1:1995520/93‑1 (MQ=255)

                                                              gcGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGGCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTcaca                           >  2:802951/1‑93 (MQ=255)

                                                                gggggTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATa                         >  2:2378646/1‑93 (MQ=255)

                                                                 ggggTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAg                        >  2:1971830/1‑93 (MQ=255)

                                                                 ggggTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAg                        >  1:2034136/1‑93 (MQ=255)

                                                                 ggggTTGTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAg                        <  1:3156322/93‑1 (MQ=255)

                                                                  gggTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACTGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGc                       <  2:4621683/93‑1 (MQ=255)

                                                                   ggTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCg                      <  1:1463968/93‑1 (MQ=255)

                                                                    gTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCgg                     >  2:3292197/1‑93 (MQ=255)

                                                                    gTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCgg                     <  2:2641930/93‑1 (MQ=255)

                                                                     ttttttGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGt                    >  2:1440537/1‑93 (MQ=255)

                                                                          tGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTcc               <  1:4286800/93‑1 (MQ=255)

                                                                             aTGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTccgcc            >  1:3542266/1‑93 (MQ=255)

                                                                             aTGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTccgcc            >  2:2150523/1‑93 (MQ=255)

                                                                             aTGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTccgcc            >  2:18196/1‑93 (MQ=255)

                                                                             aTGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTccgcc            <  2:1581361/93‑1 (MQ=255)

                                                                             aTGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTccgcc            >  2:3663307/1‑93 (MQ=255)

                                                                              tGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTccgccg           >  1:3933840/1‑93 (MQ=255)

                                                                              tGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTccgccg           >  1:412720/1‑93 (MQ=255)

                                                                                 gcAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGaa        <  1:4004895/93‑1 (MQ=255)

                                                                                   aaCCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACg      >  1:1391793/1‑93 (MQ=255)

                                                                                    aCCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAGCGt     >  1:777306/1‑93 (MQ=255)

                                                                                    aCCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGt     <  1:2198487/93‑1 (MQ=255)

                                                                                    aCCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGt     >  2:4490896/1‑93 (MQ=255)

                                                                                    aCCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGt     >  2:4520947/1‑93 (MQ=255)

                                                                                     cccAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTg    <  1:2462350/93‑1 (MQ=255)

                                                                                      ccAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTgg   <  1:1063541/93‑1 (MQ=255)

                                                                                      ccAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTgg   >  1:1900520/1‑93 (MQ=255)

                                                                                       cAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTggg  <  2:3968739/93‑1 (MQ=255)

                                                                                       cAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTggg  >  2:2778006/1‑93 (MQ=255)

GAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGG  >  NC_000913/1184394‑1184573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`