Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,184,489 A→G V110A (GTG→GCG)  potA ← ATP‑binding component of spermidine/putrescine transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,184,4890AG99.3% 467.0 / NA 149V110A (GTG→GCG) potAATP‑binding component of spermidine/putrescine transport
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (72/76);  total (72/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 152 total aligned reads displayed.

AAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATG  >  NC_000913/1184399‑1184578
                                                                                          |                                                                                         
 aGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCAt                                                                                        <  2:2095316/93‑1 (MQ=255)
    tttCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCAtttt                                                                                     >  1:3534423/1‑93 (MQ=255)
     ttCCAGCTCCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTc                                                                                    <  2:4270165/93‑1 (MQ=255)
        cAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGaa                                                                                 <  2:1119520/93‑1 (MQ=255)
         aaCTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAac                                                                                >  1:3187567/1‑93 (MQ=255)
         aaCTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAac                                                                                >  1:182332/1‑93 (MQ=255)
         aaCTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAac                                                                                >  1:1899220/1‑93 (MQ=255)
          aCTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAaca                                                                               >  2:1449846/1‑93 (MQ=255)
          aCTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAaca                                                                               <  1:2663988/93‑1 (MQ=255)
          aCTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAaca                                                                               <  2:476904/93‑1 (MQ=255)
          aCTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGGTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAaca                                                                               >  2:3872000/1‑93 (MQ=255)
            tGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACg                                                                             <  1:1961608/93‑1 (MQ=255)
             gCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACgg                                                                            <  2:400551/93‑1 (MQ=255)
             gCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACgg                                                                            <  2:3262922/93‑1 (MQ=255)
               aCCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTc                                                                          >  2:2103403/1‑93 (MQ=255)
               aCCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTc                                                                          >  1:2250463/1‑93 (MQ=255)
                ccATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCa                                                                         <  1:817443/93‑1 (MQ=255)
                   tCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCAtgt                                                                      >  2:2597183/1‑93 (MQ=255)
                   tCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCAtgt                                                                      <  2:1237288/93‑1 (MQ=255)
                   tCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCAtgt                                                                      <  2:2237311/93‑1 (MQ=255)
                   tCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGATTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCAtgt                                                                      >  1:627373/1‑93 (MQ=255)
                     cGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTgg                                                                    >  2:3353655/1‑93 (MQ=255)
                     cGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTgg                                                                    <  1:373219/93‑1 (MQ=255)
                       cAGTGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTgggg                                                                  <  2:3024004/93‑1 (MQ=255)
                       cAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTgggg                                                                  >  2:1630665/1‑93 (MQ=255)
                        aGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTggggg                                                                 >  2:4320027/1‑93 (MQ=255)
                         gggCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGa                                                                >  1:939945/1‑93 (MQ=255)
                         gggCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGa                                                                >  1:4283586/1‑93 (MQ=255)
                          ggCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGaa                                                               >  2:2359899/1‑93 (MQ=255)
                           gCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGaaa                                                              >  1:1410862/1‑93 (MQ=255)
                            cTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGaatg                                                             >  1:2763737/1‑91 (MQ=255)
                            cTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGaaaa                                                             <  1:1722084/93‑1 (MQ=255)
                             ttCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAg                                                            >  2:1251632/1‑93 (MQ=255)
                              tCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAgg                                                           >  1:3316048/1‑92 (MQ=255)
                              tCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGt                                                           <  2:4240501/93‑1 (MQ=255)
                                cATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGc                                                         <  2:4240601/93‑1 (MQ=255)
                                  tCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGt                                                       >  1:1707110/1‑93 (MQ=255)
                                   cACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTa                                                      >  2:3666845/1‑93 (MQ=255)
                                   cACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTa                                                      <  1:2743045/93‑1 (MQ=255)
                                   cACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTa                                                      <  1:4254458/93‑1 (MQ=255)
                                    aCGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAg                                                     >  1:263826/1‑93 (MQ=255)
                                     cgcgGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGc                                                    <  2:4151988/93‑1 (MQ=255)
                                     cgcgGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGc                                                    <  1:990102/93‑1 (MQ=255)
                                     cgcgGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGc                                                    <  1:2934718/93‑1 (MQ=255)
                                     cgcgGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGc                                                    <  1:254083/93‑1 (MQ=255)
                                        ggggCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCttt                                                 <  1:3958484/93‑1 (MQ=255)
                                        ggggCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCttt                                                 >  1:4673014/1‑93 (MQ=255)
                                          ggCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCATCCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTgg                                               <  2:1393673/93‑1 (MQ=255)
                                            cGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGaa                                             <  1:3649159/93‑1 (MQ=255)
                                              tAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGaaaa                                           >  1:3006347/1‑93 (MQ=255)
                                              tAATTTCAGCAGCGGGGGGTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAATAGTGCGTAGGTTTGGaaaa                                           >  2:3467097/1‑93 (MQ=255)
                                                aTTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACa                                         <  1:208624/93‑1 (MQ=255)
                                                 tttCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAg                                        <  2:2133262/93‑1 (MQ=255)
                                                 tttCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAg                                        <  2:2276210/93‑1 (MQ=255)
                                                   tCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAgtg                                      <  2:3174152/93‑1 (MQ=255)
                                                     agcagcGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGtt                                    <  1:952545/93‑1 (MQ=255)
                                                       cagcGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTca                                  <  1:4617716/93‑1 (MQ=255)
                                                         gcGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTcaca                                <  1:1995520/93‑1 (MQ=255)
                                                         gcGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTcaca                                <  1:2181290/93‑1 (MQ=255)
                                                         gcGGGGGTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGGCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTcaca                                >  2:802951/1‑93 (MQ=255)
                                                           gggggTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATa                              <  2:1544471/93‑1 (MQ=255)
                                                            ggggTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAg                             >  2:2495539/1‑93 (MQ=255)
                                                            ggggTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAg                             >  2:1971830/1‑93 (MQ=255)
                                                            ggggTTGTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAg                             <  1:3156322/93‑1 (MQ=255)
                                                             gtgTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGAGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGc                            <  1:2520285/91‑1 (MQ=255)
                                                             gggTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACTGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGc                            <  2:4621683/93‑1 (MQ=255)
                                                              ggTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCg                           <  1:1463968/93‑1 (MQ=255)
                                                              ggTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCg                           <  1:1761468/93‑1 (MQ=255)
                                                              ggTTTTTTGCATGCGCAACCCAAGGGACGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAAAAGTGTTCACATAGCg                           >  1:3865127/1‑93 (MQ=255)
                                                               gTTTTTTGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCgg                          >  2:3292197/1‑93 (MQ=255)
                                                                ttttttGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGt                         <  2:2660372/93‑1 (MQ=255)
                                                                     tGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTcc                    >  1:2205028/1‑93 (MQ=255)
                                                                     tGCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTcc                    <  1:2177687/93‑1 (MQ=255)
                                                                      gCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTccg                   >  1:2740336/1‑93 (MQ=255)
                                                                      gCATGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTccg                   >  1:4384728/1‑93 (MQ=255)
                                                                        aTGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTccgcc                 <  2:1581361/93‑1 (MQ=255)
                                                                        aTGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTccgcc                 >  2:3663307/1‑93 (MQ=255)
                                                                        aTGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTccgcc                 >  1:3542266/1‑93 (MQ=255)
                                                                        aTGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTccgcc                 >  2:2150523/1‑93 (MQ=255)
                                                                        aTGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTccgcc                 >  2:18196/1‑93 (MQ=255)
                                                                         tGCGCAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTccgccg                >  1:3933840/1‑93 (MQ=255)
                                                                          gcgcAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCgg               <  1:3284609/93‑1 (MQ=255)
                                                                            gcAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGaa             <  1:4004895/93‑1 (MQ=255)
                                                                            gcAACCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGaa             >  1:1849143/1‑93 (MQ=255)
                                                                              aaCCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACg           <  1:2042524/93‑1 (MQ=255)
                                                                               aCCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAGCGt          >  1:777306/1‑93 (MQ=255)
                                                                               aCCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGt          >  2:4490896/1‑93 (MQ=255)
                                                                               aCCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGt          >  2:4520947/1‑93 (MQ=255)
                                                                               aCCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGt          <  1:1259759/93‑1 (MQ=255)
                                                                               aCCCAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGt          >  1:235122/1‑93 (MQ=255)
                                                                                cccAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTg         <  1:2462350/93‑1 (MQ=255)
                                                                                 ccAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTgg        <  1:1063541/93‑1 (MQ=255)
                                                                                  cAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTggg       <  2:3968739/93‑1 (MQ=255)
                                                                                  cAAGGGCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTggg       >  2:2778006/1‑93 (MQ=255)
                                                                                   aagggCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGt      >  1:826823/4‑93 (MQ=255)
                                                                                   aagggCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGt      >  1:2897248/4‑93 (MQ=255)
                                                                                   aagggCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGCGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTgggg      >  1:3075739/4‑92 (MQ=255)
                                                                                     gggCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGa    <  2:1010593/92‑1 (MQ=255)
                                                                                       gCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATg  >  1:777112/1‑93 (MQ=255)
                                                                                       gCCGCATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATg  <  1:4474739/93‑1 (MQ=255)
                                                                                          |                                                                                         
AAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATG  >  NC_000913/1184399‑1184578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑