BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	111,893	A→G	V236A (GTA→GCA)	yacF ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	111,893	0	A	G	96.8%	555.2 / 4.1	188	V236A (GTA→GCA)	yacF	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (2/4); new base G (91/91); total (93/95)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 192 total aligned reads displayed.

GTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGC  >  NC_000913/111803‑111982

gTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGcc                                                                                         <  2:1495925/93‑1 (MQ=255)

gTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGcc                                                                                         >  1:2854837/1‑93 (MQ=255)

  tttCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTg                                                                                       <  1:1143495/93‑1 (MQ=255)

     cccGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTcc                                                                                    <  2:575075/93‑1 (MQ=255)

     cccGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTcc                                                                                    <  2:3423385/93‑1 (MQ=255)

          aGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGtttt                                                                               >  2:3605993/1‑93 (MQ=255)

           ggTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTc                                                                              <  2:4055829/93‑1 (MQ=255)

            gTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCa                                                                             >  1:130663/1‑93 (MQ=255)

            gTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCa                                                                             >  1:2190948/1‑93 (MQ=255)

             ttGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAg                                                                            <  2:3848217/93‑1 (MQ=255)

             ttGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAg                                                                            <  1:1739371/93‑1 (MQ=255)

              tGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAgt                                                                           <  2:4511048/93‑1 (MQ=255)

              tGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAgt                                                                           <  1:4628930/93‑1 (MQ=255)

                 gCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGATCCGGTGCCTGTCCGTTTTCAGTGTc                                                                        >  1:4162437/1‑93 (MQ=255)

                  cAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTcc                                                                       <  1:4390687/93‑1 (MQ=255)

                   aaTTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCa                                                                      <  2:157617/93‑1 (MQ=255)

                   aaTTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCa                                                                      >  2:2806438/1‑93 (MQ=255)

                    aTTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAg                                                                     >  2:3547931/1‑93 (MQ=255)

                    aTTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAg                                                                     >  2:1612675/1‑93 (MQ=255)

                    aTTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAg                                                                     >  1:975872/1‑93 (MQ=255)

                     ttCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGc                                                                    <  2:3303353/93‑1 (MQ=255)

                     ttCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGc                                                                    >  1:2434170/1‑93 (MQ=255)

                      tCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCg                                                                   <  1:2230226/93‑1 (MQ=255)

                         ccGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCa                                                                >  2:4473823/1‑93 (MQ=255)

                          cGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCAt                                                               >  1:3559857/1‑93 (MQ=255)

                          cGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCAt                                                               >  1:1063841/1‑93 (MQ=255)

                           gTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATa                                                              >  2:1375279/1‑93 (MQ=255)

                           gTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATa                                                              >  1:3801808/1‑93 (MQ=255)

                           gTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATa                                                              >  2:2718671/1‑93 (MQ=255)

                           gTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATa                                                              >  1:4041570/1‑93 (MQ=255)

                           gTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTATTCAGTGTCCAGCGGCATa                                                              >  2:3183031/1‑93 (MQ=255)

                           gTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGGTTTCAGTGTCCAGCGGCATa                                                              >  2:3183032/1‑93 (MQ=255)

                           gTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATa                                                              >  1:1567188/1‑93 (MQ=255)

                           gTAATAGTTTCTGACATTTCTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATa                                                              >  2:4565769/1‑93 (MQ=255)

                            tAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATaa                                                             >  2:2027344/1‑93 (MQ=255)

                            tAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATaa                                                             >  2:3017062/1‑93 (MQ=255)

                            tAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATaa                                                             >  1:3467265/1‑93 (MQ=255)

                              aTAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATaaaa                                                           <  2:4126013/93‑1 (MQ=255)

                              aTAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCAAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATaaaa                                                           <  1:3346229/93‑1 (MQ=255)

                              aTAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTGCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATaaaa                                                           <  2:1510516/93‑1 (MQ=255)

                                 gTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGa                                                        <  2:233566/93‑1 (MQ=255)

                                  tttCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGaa                                                       >  2:1471210/1‑93 (MQ=255)

                                  tttCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGaa                                                       >  2:3965989/1‑93 (MQ=255)

                                  tttCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGaa                                                       >  2:279369/1‑93 (MQ=255)

                                     cTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATgg                                                    <  1:4141082/93‑1 (MQ=255)

                                      tGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGc                                                   >  1:2025090/1‑93 (MQ=255)

                                       gACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCa                                                  >  1:1889770/1‑93 (MQ=255)

                                       gACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCa                                                  <  2:4672653/93‑1 (MQ=255)

                                       gACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCa                                                  <  2:718903/93‑1 (MQ=255)

                                       gACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCa                                                  >  1:941614/1‑93 (MQ=255)

                                            ttttACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACg                                             <  1:3365186/93‑1 (MQ=255)

                                              ttACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGc                                           <  1:4011374/93‑1 (MQ=255)

                                                aCTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGctc                                         <  2:815104/93‑1 (MQ=255)

                                                aCTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGctc                                         >  1:3275788/1‑93 (MQ=255)

                                                aCTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGctc                                         <  1:3611016/93‑1 (MQ=255)

                                                aCTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGctc                                         <  1:3309814/93‑1 (MQ=255)

                                                 cTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGctct                                        <  1:1512893/93‑1 (MQ=255)

                                                   ccTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCttc                                      >  2:1048655/1‑92 (MQ=255)

                                                   ccTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTa                                      <  2:2394499/93‑1 (MQ=255)

                                                   ccTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTa                                      <  1:2614649/93‑1 (MQ=255)

                                                    cTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTAt                                     <  2:4216132/93‑1 (MQ=255)

                                                    cTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTAt                                     <  1:3334741/93‑1 (MQ=255)

                                                     ttAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATg                                    >  1:2882017/1‑93 (MQ=255)

                                                     ttAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATg                                    >  1:3912204/1‑93 (MQ=255)

                                                     ttAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATg                                    >  1:1264947/1‑93 (MQ=255)

                                                      tAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGt                                   <  1:2967400/93‑1 (MQ=255)

                                                       aGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTc                                  <  1:901531/93‑1 (MQ=255)

                                                        gCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTcc                                 <  1:2731729/93‑1 (MQ=255)

                                                        gCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTcc                                 >  2:173277/1‑93 (MQ=255)

                                                        gCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTcc                                 >  2:4280703/1‑93 (MQ=255)

                                                         cAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCg                                <  2:2295084/93‑1 (MQ=255)

                                                          aaCAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCgg                               >  2:1919223/1‑93 (MQ=255)

                                                              ggCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAAt                           <  1:638225/93‑1 (MQ=255)

                                                               gCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAAtt                          <  1:2345382/93‑1 (MQ=255)

                                                               gCCAGTTCGAAAACCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAAtt                          <  2:916139/93‑1 (MQ=255)

                                                                ccAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAAttt                         <  2:873020/93‑1 (MQ=255)

                                                                ccAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAAttt                         >  1:428925/1‑93 (MQ=255)

                                                                  aGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGc                       <  2:2457269/93‑1 (MQ=255)

                                                                  aGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGc                       >  2:4145603/1‑93 (MQ=255)

                                                                   gTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCg                      >  2:261247/1‑93 (MQ=255)

                                                                   gTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCg                      <  2:634293/93‑1 (MQ=255)

                                                                    ttCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCgg                     <  2:474972/93‑1 (MQ=255)

                                                                    ttCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCgg                     <  1:4122470/93‑1 (MQ=255)

                                                                      cGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGAt                   <  1:1108379/93‑1 (MQ=255)

                                                                       gAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATa                  >  1:2746942/1‑93 (MQ=255)

                                                                         aaTCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATaaa                <  2:2143020/93‑1 (MQ=255)

                                                                          aTCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAg               >  1:706003/1‑93 (MQ=255)

                                                                          aTCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAg               <  1:545143/93‑1 (MQ=255)

                                                                          aTCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAg               >  1:3157174/1‑93 (MQ=255)

                                                                           tCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGc              <  1:1352625/93‑1 (MQ=255)

                                                                           tCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGc              <  2:3260442/93‑1 (MQ=255)

                                                                           tCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAAGTTGCGGATAAAGc              >  1:3770595/1‑93 (MQ=255)

                                                                           tCCAGACGTTCCGGTGCCTGGCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGc              <  1:3816855/93‑1 (MQ=255)

                                                                                 cGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGaa        <  1:845559/93‑1 (MQ=255)

                                                                                  gTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAAt       >  2:1975760/1‑93 (MQ=255)

                                                                                   ttCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATc      >  1:4557422/1‑93 (MQ=255)

                                                                                   ttCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATc      >  2:180859/1‑93 (MQ=255)

                                                                                    tCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCg     >  2:1326487/1‑93 (MQ=255)

                                                                                     ccGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGa    <  2:1852611/93‑1 (MQ=255)

                                                                                       ggTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGc  >  1:880423/1‑93 (MQ=255)

GTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGC  >  NC_000913/111803‑111982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`