BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,220,610	A→G	E61G (GAG→GGG)	b1170 →	putative part of putative ATP‑binding component of a transport system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,220,610	0	A	G	100.0%	368.3 / NA	118	E61G (GAG→GGG)	b1170	putative part of putative ATP‑binding component of a transport system
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (53/65); total (53/65)

Only 100 of 120 total aligned reads displayed.

TGGCAGCTGCTAACACTCTTTTTGTTATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACA‑GGA‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGACCTCGCA  >  NC_000913/1220526‑1220694

tGGCAGCTGCTAACACTCTTTTTGTTATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCa                                                                               >  1:2767164/1‑93 (MQ=255)

 ggCAGCTGCTAACCCTCTTTTTGTTATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCaa                                                                              <  2:2711432/93‑1 (MQ=255)

   cAGCTGCTAACACTCTTTTTGTTATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGc                                                                            >  1:1720444/1‑93 (MQ=255)

    aGCTGCTAACACTCTTTTTGTTATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGcc                                                                           <  1:4009521/93‑1 (MQ=255)

    aGCTGCTAACACTCTTTTTGTTATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGcc                                                                           >  2:2368522/1‑93 (MQ=255)

     gctgctAACACTCTTTTTGTTATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCg                                                                          >  1:2635111/1‑93 (MQ=255)

       tgctAACACTCTTTTTGTTATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGAc                                                                        >  1:1095329/1‑93 (MQ=255)

       tgctAACACTCTTTTTGTTATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGAc                                                                        <  2:1704466/93‑1 (MQ=255)

          tAACACTCTTTTTGTTATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACttt                                                                     >  1:1323196/1‑93 (MQ=255)

          tAACACTCTTTTTGTTATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACttt                                                                     <  1:4082086/93‑1 (MQ=255)

          tAACACTCTTTTTGTTATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACttt                                                                     <  1:416645/93‑1 (MQ=255)

           aaCACTCTTTTTGTTATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTg                                                                    <  2:59644/93‑1 (MQ=255)

           aaCACTCTTTTTGTTATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTg                                                                    <  2:4334228/93‑1 (MQ=255)

            acacTCTTTTTGTTATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTgg                                                                   >  2:1963243/1‑93 (MQ=255)

             ctctctTTTTGTTATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGc                                                                  <  1:1082227/91‑1 (MQ=255)

              cctctTTTTGTTATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCt                                                                 <  1:3041177/92‑1 (MQ=255)

              acTCTTTTTGTTATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCt                                                                 <  2:2900482/93‑1 (MQ=255)

               ctctTTTTGTTATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTa                                                                <  2:2427509/93‑1 (MQ=255)

               ctctTTTTGTTATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTa                                                                >  1:3261740/1‑93 (MQ=255)

                tctTTTTGTTATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTAc                                                               <  2:842330/93‑1 (MQ=255)

                tctTTTTGTTATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACCGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTAc                                                               <  2:3673234/93‑1 (MQ=255)

                  tttttGTTATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGt                                                             >  2:1149621/1‑93 (MQ=255)

                  tttttGTTATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGt                                                             >  1:3951787/1‑93 (MQ=255)

                  tttttGTTATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACA‑GGGGGCGTTCAAGCCGACTTTGGCTACGt                                                             <  2:4451590/93‑1 (MQ=255)

                   ttttGTTATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTc                                                            >  1:2812810/1‑93 (MQ=255)

                    tttGTTATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCa                                                           >  2:2340323/1‑93 (MQ=255)

                     ttGTTATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCaa                                                          <  2:598048/93‑1 (MQ=255)

                       gTTATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGGCAAAt                                                        >  2:2265244/1‑93 (MQ=255)

                        ttATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAAtt                                                       <  2:4582462/93‑1 (MQ=255)

                        ttATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAAtt                                                       >  1:3960166/1‑93 (MQ=255)

                         tATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTg                                                      <  2:829259/93‑1 (MQ=255)

                          aTGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTgg                                                     <  1:1635429/93‑1 (MQ=255)

                           tGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGc                                                    <  2:1701956/93‑1 (MQ=255)

                            gCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCg                                                   >  1:3325047/1‑93 (MQ=255)

                              gTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCggg                                                 >  1:3401898/1‑93 (MQ=255)

                              gTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCggg                                                 >  1:1322377/1‑93 (MQ=255)

                               tCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCgggg                                                >  2:396868/1‑92 (MQ=255)

                                cTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCa                                               <  2:210897/93‑1 (MQ=255)

                                  gAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATa                                             >  1:157483/1‑93 (MQ=255)

                                  gAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATa                                             >  1:1727984/1‑93 (MQ=255)

                                   aaCGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATaa                                            <  1:4625105/93‑1 (MQ=255)

                                   aaCGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATaa                                            >  1:3059049/1‑93 (MQ=255)

                                   aaCGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACA‑GGGGGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATaa                                            <  1:4119408/93‑1 (MQ=255)

                                    aCGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAAt                                           <  1:3680966/93‑1 (MQ=255)

                                    aCGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAAt                                           <  1:4664440/93‑1 (MQ=255)

                                      gACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGc                                         >  1:1483801/1‑93 (MQ=255)

                                      gACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGc                                         <  2:3922701/93‑1 (MQ=255)

                                      gACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGc                                         <  1:4640973/93‑1 (MQ=255)

                                      gACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGc                                         <  2:2183461/93‑1 (MQ=255)

                                         cGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTg                                      <  1:585299/93‑1 (MQ=255)

                                         cGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTg                                      <  2:1255586/93‑1 (MQ=255)

                                         cGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTg                                      <  2:1255584/93‑1 (MQ=255)

                                         cGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTg                                      >  1:81495/1‑93 (MQ=255)

                                         cGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTg                                      <  1:1820664/93‑1 (MQ=255)

                                         cGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTg                                      <  2:2358946/93‑1 (MQ=255)

                                         cGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTg                                      <  1:1710386/93‑1 (MQ=255)

                                          gTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCCgg                                     >  2:3368816/1‑93 (MQ=255)

                                              gggTGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCggg                                 >  1:1110050/1‑91 (MQ=255)

                                                 tGAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACa                              <  2:127876/93‑1 (MQ=255)

                                                  gAAACGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAg                             <  2:3004779/93‑1 (MQ=255)

                                                   aaaCGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGc                            <  1:1243220/93‑1 (MQ=255)

                                                    aaCGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCa                           <  1:1081018/93‑1 (MQ=255)

                                                    aaCGCGCTACATCGATCCTGTAACTGAACA‑GGGGGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCa                           >  1:2785286/1‑93 (MQ=255)

                                                     aCGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCaa                          <  1:782595/93‑1 (MQ=255)

                                                     aCGCGCTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCaa                          >  2:2981898/1‑93 (MQ=255)

                                                       gcgcTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACg                        >  1:2559025/1‑93 (MQ=255)

                                                       gcgcTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACg                        >  2:3126683/1‑93 (MQ=255)

                                                        cgcTACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACgg                       <  1:2973837/93‑1 (MQ=255)

                                                           tACATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACa                    >  1:2879745/1‑93 (MQ=255)

                                                            aCATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAg                   <  2:837391/93‑1 (MQ=255)

                                                             cATCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGt                  <  1:1990894/93‑1 (MQ=255)

                                                              aTCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGtt                 >  2:3998916/1‑93 (MQ=255)

                                                               tCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTg                >  1:4263774/1‑93 (MQ=255)

                                                               tCGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTg                <  2:490500/93‑1 (MQ=255)

                                                                cGATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTga               <  1:2909926/93‑1 (MQ=255)

                                                                 gATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTgag              >  1:1340055/1‑93 (MQ=255)

                                                                 gATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTgag              <  1:2307940/93‑1 (MQ=255)

                                                                 gATCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGCCTACGTCAAATTGGCGGGCATAATGACTGGCGTGACAGCAACGGACAGTTgag              >  1:3928453/1‑93 (MQ=255)

                                                                   tCCTGTAACTGAGCAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGaa            <  1:4491455/93‑1 (MQ=255)

                                                                   tCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGaa            >  2:3134024/1‑93 (MQ=255)

                                                                   tCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGaa            >  2:752413/1‑93 (MQ=255)

                                                                   tCCTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGAGGGCATCATGACTGGCGTGACAGCAACGGACCGTTGAGaa            >  2:420776/1‑93 (MQ=255)

                                                                    ccTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCCACGGACAGTTGAGAAc           >  1:498068/1‑93 (MQ=255)

                                                                    ccTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAAc           >  2:3620866/1‑93 (MQ=255)

                                                                    ccTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAAc           <  1:4615577/93‑1 (MQ=255)

                                                                     cTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACg          >  1:1890504/1‑93 (MQ=255)

                                                                     cTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACg          <  2:4340129/93‑1 (MQ=255)

                                                                     cTGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACg          >  2:3971259/1‑93 (MQ=255)

                                                                      tgcaaCTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGa         >  1:3806162/4‑93 (MQ=255)

                                                                      tGTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGa         <  2:3502772/93‑1 (MQ=255)

                                                                       gTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGAc        <  2:4183097/93‑1 (MQ=255)

                                                                       gTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGAc        <  2:1951308/93‑1 (MQ=255)

                                                                       gTAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGAc        <  1:3632720/93‑1 (MQ=255)

                                                                        tAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGAcc       <  2:796590/93‑1 (MQ=255)

                                                                        tAACTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGAcc       <  2:103695/93‑1 (MQ=255)

                                                                          aCTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGACCTc     <  1:2788898/93‑1 (MQ=255)

                                                                          aCTGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGACCTc     >  1:4097555/1‑93 (MQ=255)

                                                                            tGAACAGGGG‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGACCTCGc   >  2:518230/1‑93 (MQ=255)

                                                                             gAACA‑GGGGGCGTTCAAGCCGACTTTGGCTACGTCAAATTTGCGGGCATAATGACTGGCGTGAGAGCAACGGACAGTTGAGAACGGCCTCGCa  >  1:1328013/1‑93 (MQ=255)

                                                                             gAACA‑GGGGGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGACCTCGCa  <  1:589799/93‑1 (MQ=255)

TGGCAGCTGCTAACACTCTTTTTGTTATGCGTCTGAACGACCGTGCGGGTGAAACGCGCTACATCGATCCTGTAACTGAACA‑GGA‑GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGACCTCGCA  >  NC_000913/1220526‑1220694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`