BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,273,390	T→C	R81R (CGT→CGC)	ychP →	putative factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,273,390	0	T	C	99.2%	406.2 / ‑5.2	132	R81R (CGT→CGC)	ychP	putative factor
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (58/73); total (58/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 136 total aligned reads displayed.

AATCAGCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGTGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATAATGGGTTGGTGAG  >  NC_000913/1273301‑1273479

aaTCAGCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGa                                                                                        >  1:2700139/1‑93 (MQ=255)

aaTCAGCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGa                                                                                        <  2:856273/93‑1 (MQ=255)

aaTCAGCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGa                                                                                        >  2:3444138/1‑93 (MQ=255)

 aTCAGCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGaa                                                                                       <  1:4518050/93‑1 (MQ=255)

  tCAGCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAg                                                                                      >  1:4216852/1‑93 (MQ=255)

  tCAGCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAg                                                                                      >  2:184073/1‑93 (MQ=255)

  tCAGCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAg                                                                                      >  1:1793675/1‑93 (MQ=255)

   cAGCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGc                                                                                     <  2:2492627/93‑1 (MQ=255)

    aGCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCt                                                                                    <  1:2597563/93‑1 (MQ=255)

    aGCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCCGAAGCt                                                                                    >  2:179880/1‑93 (MQ=255)

     gCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTg                                                                                   >  1:3210361/1‑93 (MQ=255)

     gCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTg                                                                                   <  2:2098601/93‑1 (MQ=255)

       aCGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGCAGCTGGt                                                                                 >  2:2294226/1‑93 (MQ=255)

       aCGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGt                                                                                 <  2:800107/93‑1 (MQ=255)

        cGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGtt                                                                                >  2:3614041/1‑93 (MQ=255)

         gTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGttt                                                                               <  1:1186363/93‑1 (MQ=255)

          tAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGtgtg                                                                              >  2:172141/1‑93 (MQ=255)

          tAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTtg                                                                              >  2:341932/1‑93 (MQ=255)

           agagTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTtgt                                                                             <  1:1142098/93‑1 (MQ=255)

            gagTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTtgtg                                                                            >  2:125133/1‑93 (MQ=255)

            gagTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTtgtg                                                                            >  1:3153421/1‑93 (MQ=255)

            gagTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTtgtg                                                                            >  1:3243341/1‑93 (MQ=255)

              gTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGcc                                                                          <  2:1230880/93‑1 (MQ=255)

              gTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGcc                                                                          <  1:3370320/93‑1 (MQ=255)

              gTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGcc                                                                          <  2:2334247/93‑1 (MQ=255)

               tCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCg                                                                         >  2:1478744/1‑93 (MQ=255)

               tCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCg                                                                         <  1:1542798/93‑1 (MQ=255)

               tCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCg                                                                         >  1:3266703/1‑93 (MQ=255)

               tCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGGGCCg                                                                         >  2:548507/1‑93 (MQ=255)

                 cTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGtt                                                                       >  2:1016855/1‑93 (MQ=255)

                 cTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGtt                                                                       >  1:108211/1‑93 (MQ=255)

                   ggCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTGACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTAc                                                                     >  1:3050407/1‑93 (MQ=255)

                   ggCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTAc                                                                     <  2:844306/93‑1 (MQ=255)

                      tATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAg                                                                  <  2:2405301/93‑1 (MQ=255)

                       aTCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGa                                                                 >  2:313017/1‑93 (MQ=255)

                       aTCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGa                                                                 >  2:975391/1‑93 (MQ=255)

                          aCCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATaa                                                              >  2:2132365/1‑93 (MQ=255)

                          aCCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATaa                                                              <  1:2857234/93‑1 (MQ=255)

                          aCCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATaa                                                              <  2:309151/93‑1 (MQ=255)

                           ccGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAAt                                                             >  2:1277668/1‑93 (MQ=255)

                            cGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGCTAATg                                                            >  1:350903/1‑93 (MQ=255)

                            cGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATg                                                            <  1:488124/93‑1 (MQ=255)

                              tGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGAt                                                          >  2:3482708/1‑93 (MQ=255)

                               ggggAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGGTTGTGCCGTTACAAGATGATGATc                                                         >  1:2954721/1‑93 (MQ=255)

                                   aaaTGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTa                                                     <  1:1350785/93‑1 (MQ=255)

                                   aaaTGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTa                                                     <  1:2650338/93‑1 (MQ=255)

                                   aaaTGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTa                                                     <  2:4234245/93‑1 (MQ=255)

                                   aaaTGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTa                                                     <  1:3231669/93‑1 (MQ=255)

                                   aaaTGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTa                                                     >  2:4322996/1‑93 (MQ=255)

                                    aaTGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTAt                                                    >  2:2797586/1‑93 (MQ=255)

                                    aaTGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTAt                                                    <  1:4640285/93‑1 (MQ=255)

                                    aaTGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTAt                                                    >  2:4620734/1‑93 (MQ=255)

                                     aTGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTAtc                                                   >  2:3487175/1‑93 (MQ=255)

                                       gCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTAtctc                                                 <  1:1940237/93‑1 (MQ=255)

                                       gCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTAtctc                                                 <  2:2153496/93‑1 (MQ=255)

                                           gtgtTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCt                                             >  1:1591012/1‑93 (MQ=255)

                                           gtgtTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCt                                             >  1:1423938/1‑93 (MQ=255)

                                            tgtTGACGTCAAAGGTGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTg                                            <  2:3504030/93‑1 (MQ=255)

                                             gtTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTTCCGTTACAAGATAATAATCGTTATCTCACCTgg                                           >  2:1141025/1‑93 (MQ=255)

                                             gtTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTgg                                           >  1:338340/1‑93 (MQ=255)

                                                  cGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCa                                      <  2:2717506/93‑1 (MQ=255)

                                                  cGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCa                                      <  2:1936124/93‑1 (MQ=255)

                                                   gTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAg                                     <  2:2755483/93‑1 (MQ=255)

                                                     cAAAGTGGATAACGAAGTACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAGGATAATGATCGTTATCTCACCTGGAGCCAGCt                                   <  2:111833/93‑1 (MQ=255)

                                                     cAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCt                                   <  2:464506/93‑1 (MQ=255)

                                                      aaaGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGCAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCtt                                  >  2:2316165/1‑93 (MQ=255)

                                                      aaaGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCtt                                  <  1:2147742/93‑1 (MQ=255)

                                                      aaaGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCtt                                  <  1:1036968/93‑1 (MQ=255)

                                                      aaaGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCtt                                  <  1:4661479/93‑1 (MQ=255)

                                                       aaGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTg                                 >  1:2721832/1‑93 (MQ=255)

                                                       aaGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTg                                 <  1:2810503/93‑1 (MQ=255)

                                                         gTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGt                               <  2:4021169/93‑1 (MQ=255)

                                                         gTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGt                               <  1:654790/93‑1 (MQ=255)

                                                             aTAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTa                           <  1:3287685/93‑1 (MQ=255)

                                                              tAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTAc                          >  1:2618745/1‑93 (MQ=255)

                                                              tAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTAc                          <  1:2152905/93‑1 (MQ=255)

                                                               aaCGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACt                         >  1:255702/1‑93 (MQ=255)

                                                                aCGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTc                        >  1:2837681/1‑93 (MQ=255)

                                                                 cGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTca                       <  2:3181837/93‑1 (MQ=255)

                                                                 cGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTca                       <  1:3187097/93‑1 (MQ=255)

                                                                  gAAGGACATTTCACCGGCAGTCGCGGGAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTcag                      >  2:4382196/1‑93 (MQ=255)

                                                                  caaGGACATTTCACCGGCAGTCGTGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTcag                      <  1:1472129/92‑1 (MQ=255)

                                                                   aaGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCCGCTTGGTCTTACTcagc                     >  2:1863254/1‑93 (MQ=255)

                                                                    aGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTcagca                    <  1:1888890/93‑1 (MQ=255)

                                                                     ggACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTcagcag                   >  1:3408576/1‑93 (MQ=255)

                                                                     ggACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTcagcag                   <  1:3067568/93‑1 (MQ=255)

                                                                       aCATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGa                 <  2:1017198/93‑1 (MQ=255)

                                                                       aCATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGa                 <  1:4321017/93‑1 (MQ=255)

                                                                        cATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGAt                <  2:1108429/93‑1 (MQ=255)

                                                                        cATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGAt                <  1:2489376/93‑1 (MQ=255)

                                                                         aTTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATa               >  1:3063016/1‑93 (MQ=255)

                                                                          tttCACCGGCAGTCGCGGAGGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATaa              <  1:4146083/93‑1 (MQ=255)

                                                                          tttCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATaa              <  1:898813/93‑1 (MQ=255)

                                                                          tttCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATaa              >  1:329027/1‑93 (MQ=255)

                                                                             cACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATAATgg           <  2:194160/93‑1 (MQ=255)

                                                                               ccGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATAATgggg         >  2:162055/1‑92 (MQ=255)

                                                                               ccGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATAATGGGt         >  2:3348358/1‑93 (MQ=255)

                                                                               ccGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATAATGGGt         <  2:860712/93‑1 (MQ=255)

                                                                                   cAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATAATGGGTTGGt     <  1:3768164/93‑1 (MQ=255)

                                                                                      tCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATAATGGGTTGGTGAg  <  1:3320884/93‑1 (MQ=255)

AATCAGCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGTGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATAATGGGTTGGTGAG  >  NC_000913/1273301‑1273479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`