BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,275,100	A→G	V581A (GTC→GCC)	narX ←	NarX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,275,100	0	A	G	99.1%	316.2 / ‑5.8	105	V581A (GTC→GCC)	narX	NarX
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (63/41); minor base C (0/1); total (63/42)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 114 total aligned reads displayed.

GATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCGCGCATTATTATCATGCC  >  NC_000913/1275010‑1275188

gATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGgcc                                                                                        <  2:3169011/93‑1 (MQ=255)

  tCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGgccac                                                                                      >  2:1412490/1‑93 (MQ=255)

    gTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGccacca                                                                                    >  2:3252502/1‑93 (MQ=255)

    gTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGccacca                                                                                    >  2:2540954/1‑93 (MQ=255)

     tCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCcaccac                                                                                   >  1:4117104/1‑93 (MQ=255)

       aaTCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACtt                                                                                 >  2:3828840/1‑93 (MQ=255)

         tCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCg                                                                               >  2:4450980/1‑93 (MQ=255)

         tCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCg                                                                               <  2:3312662/93‑1 (MQ=255)

         tCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCg                                                                               >  2:1561789/1‑93 (MQ=255)

           agcagGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGt                                                                             <  2:1555609/93‑1 (MQ=255)

           agcagGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGt                                                                             >  1:1920421/1‑93 (MQ=255)

            gcagGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGtc                                                                            >  2:4202059/1‑92 (MQ=255)

              agGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGcc                                                                          <  2:946588/93‑1 (MQ=255)

              agGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGcc                                                                          >  1:2509061/1‑93 (MQ=255)

              agGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGcc                                                                          >  1:127094/1‑93 (MQ=255)

                gATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCAc                                                                        <  2:1180966/93‑1 (MQ=255)

                gATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCAc                                                                        <  1:4210295/93‑1 (MQ=255)

                 aTAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCAcc                                                                       <  2:3149361/93‑1 (MQ=255)

                  tagtagCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCt                                                                      >  1:1373742/1‑93 (MQ=255)

                   agtagCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTg                                                                     <  2:4155571/93‑1 (MQ=255)

                    gtagCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGa                                                                    >  2:3051114/1‑93 (MQ=255)

                    gtagCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGa                                                                    <  2:1516541/93‑1 (MQ=255)

                    gtagCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGa                                                                    >  2:948170/1‑93 (MQ=255)

                    gtagCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGa                                                                    >  1:1437710/1‑93 (MQ=255)

                      tgCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCAGCACTTCGGTGCCACCTGAtt                                                                  <  2:2746556/92‑1 (MQ=255)

                        ccGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCa                                                                >  2:1740302/1‑93 (MQ=255)

                        ccGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCa                                                                <  1:4206625/93‑1 (MQ=255)

                         cGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCac                                                               <  1:2934042/93‑1 (MQ=255)

                         cGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCac                                                               <  1:2490850/93‑1 (MQ=255)

                          ggTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCacg                                                              >  1:705341/1‑93 (MQ=255)

                          ggTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCAGGAATAAAGGTGGCCACCACTTCGGTGCGACCTGATTCacg                                                              >  1:2659394/1‑93 (MQ=255)

                           gTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCacga                                                             >  2:2532289/1‑93 (MQ=255)

                            ttCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCacgac                                                            >  2:4116345/1‑93 (MQ=255)

                             tCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCacgacg                                                           >  1:810314/1‑93 (MQ=255)

                                tGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGAcggcg                                                        >  2:1660375/1‑93 (MQ=255)

                                 gATTACTCATGGGAATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGAcggcgg                                                       >  2:1663925/1‑93 (MQ=255)

                                   ttACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGAc                                                     >  2:747845/1‑93 (MQ=255)

                                     aCTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGAcgc                                                   <  1:620300/93‑1 (MQ=255)

                                       tCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCgg                                                 >  2:3083483/1‑93 (MQ=255)

                                       tCATGGGTATCTCCTTGGAAGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGCCGCgg                                                 >  2:1195778/1‑93 (MQ=255)

                                        cATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGc                                                >  1:1090912/1‑93 (MQ=255)

                                          tGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCaa                                              >  1:613882/1‑93 (MQ=255)

                                          tGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCaa                                              <  1:338330/93‑1 (MQ=255)

                                           gggTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAAt                                             >  2:399604/1‑93 (MQ=255)

                                            ggTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATc                                            <  2:2776392/93‑1 (MQ=255)

                                            ggTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATc                                            >  1:2818564/1‑93 (MQ=255)

                                              tATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGc                                          >  1:1798214/1‑93 (MQ=255)

                                              tATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGc                                          >  1:4226254/1‑93 (MQ=255)

                                              tATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGc                                          >  1:2663752/1‑93 (MQ=255)

                                                tctcCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCt                                        >  2:3886679/1‑93 (MQ=255)

                                                tctcCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCt                                        <  2:4050401/93‑1 (MQ=255)

                                                     ttGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCAGTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTaa                                   >  1:1099094/1‑93 (MQ=255)

                                                      tGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTaaa                                  <  2:3709524/93‑1 (MQ=255)

                                                      tGGACGTCTGTGAAAGGTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCCATCGCCTCGTaaa                                  >  2:3020987/1‑93 (MQ=255)

                                                       ggACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAAc                                 >  1:30440/1‑93 (MQ=255)

                                                         aCGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACtt                               <  2:3698189/93‑1 (MQ=255)

                                                         aCGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACtt                               <  2:2472690/93‑1 (MQ=255)

                                                          cGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACttt                              <  2:3460699/93‑1 (MQ=255)

                                                           gTCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTg                             >  2:1964706/1‑93 (MQ=255)

                                                            tCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTgc                            >  2:1679524/1‑93 (MQ=255)

                                                            tCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTgc                            <  1:4394881/93‑1 (MQ=255)

                                                            tCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTgc                            >  2:3274939/1‑93 (MQ=255)

                                                            tCTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTgc                            <  2:4153059/93‑1 (MQ=255)

                                                             cTGTGAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTgcg                           <  1:2175762/93‑1 (MQ=255)

                                                               gtgAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCa                         >  2:1560227/1‑93 (MQ=255)

                                                               gtgAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCa                         >  1:4285369/1‑93 (MQ=255)

                                                               gtgAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCa                         >  1:339244/1‑93 (MQ=255)

                                                                tgAAAGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCAc                        >  1:523851/1‑93 (MQ=255)

                                                                  aaaGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGa                      <  2:1079610/93‑1 (MQ=255)

                                                                  aaaGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGa                      >  2:383610/1‑93 (MQ=255)

                                                                  aaaGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCATAAACTTTGCGCACGa                      >  2:3570211/1‑93 (MQ=255)

                                                                    aGTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATc                    >  1:1874237/1‑93 (MQ=255)

                                                                     gTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTGAACTTTGCGCACGATcg                   >  2:856165/1‑93 (MQ=255)

                                                                     gTTTTTTCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATcg                   >  1:3820539/1‑93 (MQ=255)

                                                                      ttttttCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATcgc                  <  1:1174589/93‑1 (MQ=255)

                                                                      gtttttCGGGAATAAAGGTGGCCACCACTGCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATcgc                  <  1:2751443/92‑1 (MQ=255)

                                                                       tttttCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATcgcg                 >  2:346920/1‑93 (MQ=255)

                                                                         tttCGGGAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCGCGCa               <  1:158268/93‑1 (MQ=255)

                                                                           tCGGGAATAAAGGTGCCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCGCGCatt             <  1:3683732/93‑1 (MQ=255)

                                                                             gggAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCGCGCattat           <  2:3339329/93‑1 (MQ=255)

                                                                             gggAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCGCGCattat           <  2:3533742/93‑1 (MQ=255)

                                                                               gAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCGCGCattcttt         >  1:4007214/1‑92 (MQ=255)

                                                                               gAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCGCGCAttatta         <  2:4281419/93‑1 (MQ=255)

                                                                               gAATAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCGCGCAttatta         <  1:33737/93‑1 (MQ=255)

                                                                                aaTAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCGCGCATtattat        >  2:497907/1‑93 (MQ=255)

                                                                                aaTAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCGCGCATtattat        >  1:2577865/1‑93 (MQ=255)

                                                                                 aTAAAGGTGGCCCCCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCGCGCATTATTATc       <  2:859516/93‑1 (MQ=255)

                                                                                  tAAAGGTGGCCCCCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCGCGCATTATTATCa      <  2:193596/93‑1 (MQ=255)

                                                                                  tAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCGCGCATTATTATCa      >  2:3373597/1‑93 (MQ=255)

                                                                                  tAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCGCGCATTATTATCa      <  1:1675183/93‑1 (MQ=255)

                                                                                  tAAAGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCGCGCATTATTATCa      <  1:1739886/93‑1 (MQ=255)

                                                                                   aaaGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCGCGCATTATTATCAt     <  2:1800059/93‑1 (MQ=255)

                                                                                   aaaGGTGGCCACCACTGCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCGCGCATTATTATCAt     <  1:2340014/93‑1 (MQ=255)

                                                                                   aaaGCTGCCCACCCCTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCGCGCATTATTATCAt     <  2:2493847/93‑1 (MQ=255)

                                                                                    aaGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCGCGCATTATTATCATg    <  2:1193696/93‑1 (MQ=255)

                                                                                    aaGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCGCGCATTATTATCATg    >  2:1379046/1‑93 (MQ=255)

                                                                                    aaGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCGCGCATTATTATCATg    >  2:2067290/1‑93 (MQ=255)

                                                                                     aGGTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCGCGCATTATTATCATGc   <  1:1190213/93‑1 (MQ=255)

                                                                                      ggTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCGCGCATTATTATCATGcc  >  1:1656975/1‑93 (MQ=255)

                                                                                      ggTGGCCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCGCGCATTATTATCATGcc  >  1:3079384/1‑93 (MQ=255)

GATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCGCGCATTATTATCATGCC  >  NC_000913/1275010‑1275188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`