BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,276,302	T→C	Q180Q (CAA→CAG)	narX ←	NarX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,276,302	0	T	C	99.2%	372.1 / ‑4.9	119	Q180Q (CAA→CAG)	narX	NarX
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (55/63); total (55/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

Only 100 of 121 total aligned reads displayed.

GCCATTTCGTTGCGCCCGCTGATGTTTGCGCGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGTTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCATTACCCGATGGACCAGTACCACTG  >  NC_000913/1276213‑1276390

gCCATTTCGTTGCGCCCGCTGATGTTTGCGCGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGt                                                                                       >  2:247005/1‑93 (MQ=255)

gCCATTTCGTTGCGCCCGCTGATGTTTGCGCGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGt                                                                                       >  2:2652508/1‑93 (MQ=255)

 ccATTTCGTTGCGCCCGCTGATGTTTGCGCGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTa                                                                                      <  1:4443463/93‑1 (MQ=255)

  cATTTCGTTGCGCCCGCTGATGTTTGCGCGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAg                                                                                     >  2:3479011/1‑93 (MQ=255)

  cATTTCGTTGCGCCCGCTGATGTTTGCGCGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAg                                                                                     >  2:4403885/1‑93 (MQ=255)

   aTTTCGTTGCGCCCGCTGATGTTTGCGCGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGc                                                                                    <  2:1074817/93‑1 (MQ=255)

   aTTTCGTTGCGCCCGCTGATGTTTGCGCGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGc                                                                                    >  1:4577900/1‑93 (MQ=255)

   aTTTCGTTGCGCCCGCTGATGTTTGCGCGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCAAGCAGTTGCCGCCACGGCTGTAGc                                                                                    <  2:398755/93‑1 (MQ=255)

    tttCGTTGCGCCCGCTGATGTTTGCGCGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCa                                                                                   <  2:3562713/93‑1 (MQ=255)

       cGTTGCGCCCGCTGATGTTTGCGCGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTc                                                                                <  1:2355996/93‑1 (MQ=255)

        gTTGCGCCCGCTGATGTTTGCGCGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTcg                                                                               <  1:2243929/93‑1 (MQ=255)

        gTTGCGCCCGCTGATGTTTGCGCGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGAAGTcg                                                                               >  2:2300718/1‑93 (MQ=255)

         ttGCGCCCGCTGATGTTTGCGCGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTcgc                                                                              >  1:893029/1‑93 (MQ=255)

         ttGCGCCCGCTGATGTTTGCGCGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTcgc                                                                              >  2:481635/1‑93 (MQ=255)

           gcgcCCGCTGATGTTTGCGCGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCgcgc                                                                            <  1:2119511/93‑1 (MQ=255)

               ccGCTGATGTTTGCGCGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGc                                                                        >  1:4383665/1‑93 (MQ=255)

                cGCTGATGTTTGCGCGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCa                                                                       >  2:756052/1‑93 (MQ=255)

                 gCTGATGTTTGCGCGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCaa                                                                      <  1:3816409/93‑1 (MQ=255)

                  cTGATGTTTGCGCGTTGGGTAAAATCGCGATGACTGACGGCCCTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAAc                                                                     <  2:2948630/93‑1 (MQ=255)

                  cTGATGTTTGCGCGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAAc                                                                     >  2:4545111/1‑93 (MQ=255)

                  cTGATGTTTGCGCGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAAc                                                                     >  2:4667/1‑93 (MQ=255)

                  cTGATGTTTGCGCGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAAc                                                                     <  2:570423/93‑1 (MQ=255)

                  cTGATGTTTGCGCGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAAc                                                                     >  1:4474798/1‑93 (MQ=255)

                   tGATGTTTGCGCGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAAcc                                                                    >  1:4326090/1‑93 (MQ=255)

                   tGATGTTTGCGCGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAAcc                                                                    >  1:2280691/1‑93 (MQ=255)

                    gATGTTTGCGCGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCa                                                                   <  2:3496459/93‑1 (MQ=255)

                      tGTTTGCGCGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGa                                                                 >  1:3241459/1‑93 (MQ=255)

                      tGTTTGCGCGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGa                                                                 >  2:2751140/1‑93 (MQ=255)

                       gTTTGCGCGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGat                                                                <  2:1728855/93‑1 (MQ=255)

                          tGCGCGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGataat                                                             <  1:4220939/93‑1 (MQ=255)

                          tGCGCGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGataat                                                             <  2:3273900/93‑1 (MQ=255)

                           gcgcGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGataata                                                            <  1:2852310/93‑1 (MQ=255)

                            ggcgTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAg                                                           <  1:1147809/92‑1 (MQ=255)

                               gTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGa                                                        <  1:1002930/93‑1 (MQ=255)

                               gTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGa                                                        >  2:404882/1‑93 (MQ=255)

                                ttGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGaa                                                       >  1:784632/1‑93 (MQ=255)

                                ttGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGaa                                                       <  2:1476077/93‑1 (MQ=255)

                                gtGGGTAAAATCGGGATGACTGACGGCCCTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGaa                                                       <  2:1132823/92‑1 (MQ=255)

                                 tGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAac                                                      >  1:3156016/1‑93 (MQ=255)

                                 tGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAac                                                      <  2:1399623/93‑1 (MQ=255)

                                  gggTAAAATCGCGATGACTGACGGCACTCGTCATTGCCAGCATTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAaca                                                     >  1:3421743/1‑93 (MQ=255)

                                   ggTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAacac                                                    >  2:470775/1‑93 (MQ=255)

                                   ggTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAacac                                                    <  2:4159242/93‑1 (MQ=255)

                                     tAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCa                                                  >  2:3209861/1‑93 (MQ=255)

                                      aaaaTCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAg                                                 <  2:3097396/93‑1 (MQ=255)

                                       aaaTCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAga                                                >  2:3812634/1‑92 (MQ=255)

                                       aaaTCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGt                                                >  2:2110048/1‑93 (MQ=255)

                                         aTCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTaa                                              <  2:259098/93‑1 (MQ=255)

                                         aTCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTaa                                              <  1:185692/93‑1 (MQ=255)

                                          tCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTaaa                                             <  2:4220528/93‑1 (MQ=255)

                                           cgcgATGACTGACGTCCCTCGCCATCGCCAGCAGTTGAAACCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTaaaa                                            <  1:2272303/93‑1 (MQ=255)

                                            gcgATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAg                                           >  1:2468320/1‑93 (MQ=255)

                                            gcgATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAg                                           <  1:2848820/93‑1 (MQ=255)

                                            gcgATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAg                                           <  1:3499572/93‑1 (MQ=255)

                                            gcgATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAg                                           <  1:490370/93‑1 (MQ=255)

                                               aTGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGc                                        >  1:979877/1‑93 (MQ=255)

                                                tgactgacGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGcc                                       <  2:3177112/93‑1 (MQ=255)

                                                tgactgacGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGcc                                       <  1:3736039/93‑1 (MQ=255)

                                                   ctgacGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATa                                    >  2:245018/1‑93 (MQ=255)

                                                    cgacGGCACTCGCCATTACCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCACATAATAGTGAACACCAGTAAAAGTGCCATaa                                   <  2:1041388/92‑1 (MQ=255)

                                                     gacGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATaaa                                  <  2:4091493/93‑1 (MQ=255)

                                                        gtcACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATAc                               <  2:3970086/91‑1 (MQ=255)

                                                        ggCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATAc                               <  2:619324/93‑1 (MQ=255)

                                                        ggCACTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATAc                               <  1:3033887/93‑1 (MQ=255)

                                                           aCTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGc                            >  2:3837773/1‑93 (MQ=255)

                                                           aCTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGc                            >  1:1223556/1‑93 (MQ=255)

                                                            cTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGcc                           >  1:456551/1‑93 (MQ=255)

                                                            cTCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGcc                           >  1:2977587/1‑93 (MQ=255)

                                                             tCGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCa                          <  2:2164505/93‑1 (MQ=255)

                                                              cGCCATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCAt                         <  2:4603447/93‑1 (MQ=255)

                                                                ccATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCATTa                       >  2:243565/1‑93 (MQ=255)

                                                                ccATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCATTa                       >  2:151930/1‑93 (MQ=255)

                                                                 cATTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCATTAc                      <  2:3574424/93‑1 (MQ=255)

                                                                  aTTGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCATTAcc                     >  1:1153600/1‑93 (MQ=255)

                                                                   ttGCCAGCAGTTGCCGCCACGGTTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCATTAccc                    <  2:2116535/93‑1 (MQ=255)

                                                                   ttGCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCATTAccc                    <  2:4028503/93‑1 (MQ=255)

                                                                     gCCAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCATTACCCGa                  <  1:3658215/93‑1 (MQ=255)

                                                                      ccAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCATTACCCGAt                 <  2:1928959/93‑1 (MQ=255)

                                                                      ccAGCAGTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCATTACCCGAt                 <  1:4479566/93‑1 (MQ=255)

                                                                        agcagTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCATTACCCGATgg               <  2:491259/93‑1 (MQ=255)

                                                                        agcagTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCATTACCCGATgg               <  2:1305934/93‑1 (MQ=255)

                                                                         gcagTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCATTACCCGATGGa              <  2:1257489/93‑1 (MQ=255)

                                                                           agTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCATTACCCGATGGAcc            <  2:2910739/93‑1 (MQ=255)

                                                                            gTTGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCATTACCCGATGGACCa           >  2:4505735/1‑93 (MQ=255)

                                                                             ttGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCATTACCCGATGGACCAg          <  1:2911761/93‑1 (MQ=255)

                                                                             ttGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCATTACCCGATGGACCAg          >  1:3660755/1‑93 (MQ=255)

                                                                             ttGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCATTACCCGATGGACCAg          >  2:1897354/1‑93 (MQ=255)

                                                                             gtGCCGCCACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCATTACCCGATGGACCAg          <  1:1013556/92‑1 (MQ=255)

                                                                               gccgccACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCATTACCCGATGGACCAGTa        <  2:1351028/93‑1 (MQ=255)

                                                                               gccgccACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCATTACCCGATGGACCAGTa        <  1:3758460/93‑1 (MQ=255)

                                                                                  gccACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCATTACCCGATGGACCAGTAccc     >  1:3697232/1‑92 (MQ=255)

                                                                                  gccACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCATTACCCGATGGACCAGTACCa     >  1:3438227/1‑93 (MQ=255)

                                                                                   ccACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCATTACCCGATGGACCAGTACCAc    <  2:1844868/93‑1 (MQ=255)

                                                                                   ccACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCATTACCCGATGGACCAGTACCAc    >  1:580000/1‑93 (MQ=255)

                                                                                   ccACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCATTACCCGATGGACCAGTACCAc    <  1:3065571/93‑1 (MQ=255)

                                                                                   ccACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCATTACCCGATGGACCAGTACCAc    <  2:767294/93‑1 (MQ=255)

                                                                                    cACGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCATTACCCGATGGAGCAGTACCACt   >  2:184222/1‑93 (MQ=255)

                                                                                     aCGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCATTACCCGATGGACCAGTACCACTg  >  2:2565995/1‑93 (MQ=255)

                                                                                     aCGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCATTACCCGATGGACCAGTACCACTg  <  1:736166/93‑1 (MQ=255)

                                                                                     aCGGCTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCATTACCCGATGGACCAGTACCACTg  >  1:4382337/1‑93 (MQ=255)

GCCATTTCGTTGCGCCCGCTGATGTTTGCGCGTTGGGTAAAATCGCGATGACTGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGTTGTAGCAGTCGCGCCCGCAACCAGATAATAGTGAACACCAGTAAAAGTGCCATAAATACCGCCATTACCCGATGGACCAGTACCACTG  >  NC_000913/1276213‑1276390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`