BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,300,534	T→C	C443C (TGT→TGC)	oppA →	OppA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,300,534	0	T	C	99.1%	357.8 / NA	115	C443C (TGT→TGC)	oppA	OppA
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (47/67); minor base A (1/0); total (48/67)
Fisher's exact test for biased strand distribution p-value = 4.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

TGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGA  >  NC_000913/1300444‑1300623

 gtAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCt                                                                                        <  2:3208972/93‑1 (MQ=255)

 gtAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCt                                                                                        >  2:1016601/1‑93 (MQ=255)

  tAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTg                                                                                       <  2:3281319/93‑1 (MQ=255)

    aaCGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGAc                                                                                     <  1:3629924/93‑1 (MQ=255)

    aaCGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGAc                                                                                     >  2:3754922/1‑93 (MQ=255)

    aaCGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGAc                                                                                     <  1:2469673/93‑1 (MQ=255)

     aCGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACt                                                                                    <  1:411693/93‑1 (MQ=255)

      cGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTa                                                                                   <  1:4436186/93‑1 (MQ=255)

        tCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACa                                                                                 >  1:3017617/1‑93 (MQ=255)

        tCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACa                                                                                 >  2:1881768/1‑93 (MQ=255)

        tCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACa                                                                                 >  2:242988/1‑93 (MQ=255)

        tCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACa                                                                                 >  2:828167/1‑93 (MQ=255)

         cAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACaa                                                                                <  2:2085840/93‑1 (MQ=255)

          aaaCTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTAATTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAAc                                                                               >  1:4559126/1‑93 (MQ=255)

               ggTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAAcc                                                                          <  1:1442259/93‑1 (MQ=255)

                  tAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAAc                                                                       <  1:1061920/93‑1 (MQ=255)

                     ccAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAActtc                                                                    >  1:2277229/1‑93 (MQ=255)

                      cAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAActtcc                                                                   >  1:577471/1‑93 (MQ=255)

                       aGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAActtcct                                                                  <  2:401296/93‑1 (MQ=255)

                       aGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAActtcct                                                                  >  2:4257961/1‑93 (MQ=255)

                       aGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAActtcct                                                                  <  2:2658884/93‑1 (MQ=255)

                       aGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAActtcct                                                                  <  2:546662/93‑1 (MQ=255)

                        ggAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAActtcctt                                                                 <  1:3062935/93‑1 (MQ=255)

                        ggAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAActtcctt                                                                 >  2:2181081/1‑93 (MQ=255)

                          aGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACttccttcc                                                               <  2:363609/93‑1 (MQ=255)

                           gTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTtccttcct                                                              <  2:3885455/93‑1 (MQ=255)

                            tGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTg                                                             >  2:1010670/1‑93 (MQ=255)

                                aaaCGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAaca                                                         >  1:2356202/1‑93 (MQ=255)

                                  aCGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACAcc                                                       >  1:2580141/1‑93 (MQ=255)

                                   cGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCa                                                      <  1:1117673/93‑1 (MQ=255)

                                   cGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCa                                                      <  2:4190294/93‑1 (MQ=255)

                                   cGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCa                                                      >  2:1712088/1‑93 (MQ=255)

                                    gTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCACCTTCCTTCCTGAACACCAt                                                     <  2:3656093/93‑1 (MQ=255)

                                     ttCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATg                                                    <  1:3748180/93‑1 (MQ=255)

                                        cTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCtt                                                 >  1:4150177/1‑93 (MQ=255)

                                        cTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCtt                                                 <  1:630050/93‑1 (MQ=255)

                                        cTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTACTTCCTGTACACAATGCtt                                                 >  2:3808445/1‑93 (MQ=255)

                                         tCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCttt                                                <  2:4625827/93‑1 (MQ=255)

                                          cGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTc                                               <  2:2807055/93‑1 (MQ=255)

                                          cGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTc                                               <  1:4258785/93‑1 (MQ=255)

                                          cGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTc                                               <  1:4543572/93‑1 (MQ=255)

                                          cGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTc                                               >  2:611410/1‑93 (MQ=255)

                                            acacCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGa                                             >  1:2860779/1‑93 (MQ=255)

                                             cacCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGaa                                            <  1:294501/93‑1 (MQ=255)

                                             cacCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGaa                                            <  2:819319/93‑1 (MQ=255)

                                               cccGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACCACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACa                                          >  1:4457986/1‑93 (MQ=255)

                                                 cGTCACCAGGGTTCTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGc                                        <  2:4324710/93‑1 (MQ=255)

                                                 cGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGc                                        <  2:1467563/93‑1 (MQ=255)

                                                 cGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGc                                        <  2:567229/93‑1 (MQ=255)

                                                  gTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCt                                       <  1:1792273/93‑1 (MQ=255)

                                                  gTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCt                                       <  2:544444/93‑1 (MQ=255)

                                                  gTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCt                                       <  2:2246781/93‑1 (MQ=255)

                                                  gTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGAGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCt                                       >  2:3826831/1‑93 (MQ=255)

                                                   tCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTc                                      <  2:1953983/93‑1 (MQ=255)

                                                    cACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCg                                     >  1:3923876/1‑93 (MQ=255)

                                                     aCCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGGGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGa                                    >  2:3963626/1‑93 (MQ=255)

                                                      ccAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGAt                                   >  1:678887/1‑93 (MQ=255)

                                                       cAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATg                                  >  1:1010911/1‑93 (MQ=255)

                                                       cAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATg                                  >  1:576425/1‑93 (MQ=255)

                                                       cAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATg                                  <  2:4041620/93‑1 (MQ=255)

                                                       cAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGCGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATg                                  >  2:4650020/1‑93 (MQ=255)

                                                         gggTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGaa                                >  1:1097740/1‑93 (MQ=255)

                                                          ggTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAAt                               <  2:2964185/93‑1 (MQ=255)

                                                           gTACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATa                              <  1:4134259/93‑1 (MQ=255)

                                                            tACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATAc                             >  2:4423661/1‑93 (MQ=255)

                                                            tACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATAc                             <  1:1041637/93‑1 (MQ=255)

                                                            tACTTTTGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATAc                             >  1:4020943/1‑93 (MQ=255)

                                                               ttttGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACcgc                          <  2:4313740/93‑1 (MQ=255)

                                                                 ttGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAAACAACTTCCTTCCTGAACAGCATGCTTTCGAACAGCTCGATGAATACCgcgc                        >  2:4596185/1‑93 (MQ=255)

                                                                  tGATGTGGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGCTGAATACCGCGCa                       >  2:45694/1‑93 (MQ=255)

                                                                     tgtgGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATta                    >  1:2001502/1‑93 (MQ=255)

                                                                     tgtgGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATta                    >  2:592488/1‑93 (MQ=255)

                                                                     tgtgGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATta                    >  1:326194/1‑93 (MQ=255)

                                                                     tgtgGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATta                    <  2:4352192/93‑1 (MQ=255)

                                                                      gtgGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATtat                   <  1:2987164/93‑1 (MQ=255)

                                                                      gtgGCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATtat                   <  1:4255506/93‑1 (MQ=255)

                                                                        ggCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATaa                 <  2:2708426/93‑1 (MQ=255)

                                                                        ggCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATaa                 <  2:825578/93‑1 (MQ=255)

                                                                        ggCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGACGAATACCGCGCATTATaa                 <  1:2685619/93‑1 (MQ=255)

                                                                         gCCCGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAag                >  1:2626031/1‑93 (MQ=255)

                                                                          cccGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAaga               >  2:2833914/1‑93 (MQ=255)

                                                                          cccGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAaga               <  1:990222/93‑1 (MQ=255)

                                                                            cGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGc             >  2:509941/1‑93 (MQ=255)

                                                                            cGTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGc             >  1:310636/1‑93 (MQ=255)

                                                                             gTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGcc            <  1:4195515/93‑1 (MQ=255)

                                                                             gTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGcc            <  2:4565950/93‑1 (MQ=255)

                                                                             gTGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGcc            <  1:529507/93‑1 (MQ=255)

                                                                              tGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGccc           <  2:1929711/93‑1 (MQ=255)

                                                                              tGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGccc           <  1:2139078/93‑1 (MQ=255)

                                                                              tGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGccc           <  2:817357/93‑1 (MQ=255)

                                                                              tGCAGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGccc           >  1:4447646/1‑93 (MQ=255)

                                                                                 aGGCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGc        >  1:1840592/1‑93 (MQ=255)

                                                                                  ggCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGcc       <  2:4039835/93‑1 (MQ=255)

                                                                                  ggCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGcc       >  2:878707/1‑93 (MQ=255)

                                                                                   gCTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCt      <  2:2176132/93‑1 (MQ=255)

                                                                                    cTGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCtt     <  1:716389/93‑1 (MQ=255)

                                                                                     tGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCttt    >  1:798522/1‑93 (MQ=255)

                                                                                     tGGTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCttt    <  2:3659895/93‑1 (MQ=255)

                                                                                      ggTGCGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTg   >  1:1986377/1‑93 (MQ=255)

                                                                                       gtgcGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGa  >  2:1067755/1‑93 (MQ=255)

TGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGA  >  NC_000913/1300444‑1300623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`