BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,302,327	T→C	V157A (GTG→GCG)	oppC →	OppC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,302,327	0	T	C	99.2%	419.8 / NA	134	V157A (GTG→GCG)	oppC	OppC
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (76/57); minor base A (1/0); total (77/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 137 total aligned reads displayed.

GTTATCTGGGCGGTAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGTGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAA  >  NC_000913/1302237‑1302416

gTTATCTGGGCGGTAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGa                                                                                         >  1:1539209/1‑93 (MQ=255)

 ttATCTGGGCGGTAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGAc                                                                                        >  2:811936/1‑93 (MQ=255)

  tATCTGGGCGGTAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGAcc                                                                                       >  2:3968825/1‑93 (MQ=255)

   aTCTGGGCGGTAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGAGACCt                                                                                      >  2:1169642/1‑93 (MQ=255)

    tCTGGGCGGTAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCtt                                                                                     >  1:2527683/1‑93 (MQ=255)

    tCTGGGCGGTAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCtt                                                                                     <  2:3024698/93‑1 (MQ=255)

       gggCGGTAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCttttt                                                                                  <  2:3972891/93‑1 (MQ=255)

       gggCGGTAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCttttt                                                                                  <  1:2088201/93‑1 (MQ=255)

                aGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCaaaa                                                                         <  1:2455670/93‑1 (MQ=255)

                  tGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACa                                                                       <  1:4239422/93‑1 (MQ=255)

                    gATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATc                                                                     <  1:3383521/93‑1 (MQ=255)

                    gATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATc                                                                     >  2:411098/1‑93 (MQ=255)

                    gATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATc                                                                     <  1:1984714/93‑1 (MQ=255)

                     aTTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATcc                                                                    >  2:3676737/1‑93 (MQ=255)

                     aTTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATcc                                                                    <  2:3818845/93‑1 (MQ=255)

                      ttCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCACAACATCct                                                                   >  2:3385701/1‑93 (MQ=255)

                      ttCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCct                                                                   <  1:218707/93‑1 (MQ=255)

                        cGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCctgc                                                                 <  1:772118/93‑1 (MQ=255)

                         ggTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCctgct                                                                >  1:1962202/1‑93 (MQ=255)

                         ggTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCctgct                                                                <  2:129256/93‑1 (MQ=255)

                            aaTGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGAt                                                             <  2:2876144/93‑1 (MQ=255)

                             atgatgCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGAtt                                                            <  2:859499/93‑1 (MQ=255)

                             atgatgCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGAtt                                                            >  1:2655474/1‑93 (MQ=255)

                              tgatgCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGAttt                                                           >  1:3915169/1‑93 (MQ=255)

                              tgatgCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGAttt                                                           >  1:240893/1‑93 (MQ=255)

                               gatgCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGAtttt                                                          >  2:2076735/1‑93 (MQ=255)

                               gatgCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGAtttt                                                          <  1:4200542/93‑1 (MQ=255)

                               gatgCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGAtttt                                                          >  1:1164133/1‑93 (MQ=255)

                                atgCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTc                                                         >  2:345008/1‑93 (MQ=255)

                                 tgCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCg                                                        >  1:592345/1‑93 (MQ=255)

                                 tgCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCg                                                        <  1:1832119/93‑1 (MQ=255)

                                 tgCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCg                                                        <  2:1760059/93‑1 (MQ=255)

                                  gCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGt                                                       <  2:875938/93‑1 (MQ=255)

                                   cGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTg                                                      <  1:1472741/93‑1 (MQ=255)

                                     tcttctGTAAAGCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGc                                                    <  2:2177706/93‑1 (MQ=255)

                                        gctgGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGAt                                                 >  1:959968/1‑93 (MQ=255)

                                        gctgGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGAt                                                 <  1:4642056/93‑1 (MQ=255)

                                         ctgGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATa                                                >  2:2579982/1‑93 (MQ=255)

                                           ggAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAACCATCCTGCTGATTTTCGTGGCGATAgg                                              >  1:391977/1‑93 (MQ=255)

                                           ggAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAgg                                              >  1:2310216/1‑93 (MQ=255)

                                            gAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGc                                             >  2:1676470/1‑93 (MQ=255)

                                            gAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGc                                             >  1:982733/1‑93 (MQ=255)

                                            gAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGc                                             >  1:2287252/1‑93 (MQ=255)

                                              aaTCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCAt                                           <  2:747657/93‑1 (MQ=255)

                                              aaTCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCAt                                           >  2:4355109/1‑93 (MQ=255)

                                              aaTCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCAt                                           >  2:1585736/1‑93 (MQ=255)

                                              aaTCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCAt                                           >  1:4102747/1‑93 (MQ=255)

                                              aaTCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCAt                                           <  1:1862840/93‑1 (MQ=255)

                                                tCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATgt                                         >  1:271859/1‑92 (MQ=255)

                                                tCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATgg                                         <  1:4271802/93‑1 (MQ=255)

                                                 ccTCACCTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGt                                        <  2:3315664/93‑1 (MQ=255)

                                                     aaCTCCTTCCCATTCATGTTCTTCGTCATTTTGCTTGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTCGCGATAGGCATGGTTTcc                                    >  2:2745704/1‑93 (MQ=255)

                                                     aaCTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTcc                                    >  1:2805880/1‑93 (MQ=255)

                                                     aaCTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTcc                                    <  2:1790926/93‑1 (MQ=255)

                                                     aaCTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTcc                                    <  2:3656886/93‑1 (MQ=255)

                                                     aaCTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTcc                                    >  1:4330583/1‑93 (MQ=255)

                                                     aaCTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTcc                                    <  1:2623483/93‑1 (MQ=255)

                                                      aCTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCct                                   >  2:2380116/1‑93 (MQ=255)

                                                      aCTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCct                                   >  1:2215430/1‑93 (MQ=255)

                                                      aCTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCct                                   >  1:1365360/1‑93 (MQ=255)

                                                      aCTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCct                                   >  1:1661214/1‑93 (MQ=255)

                                                        tCCTTCCCATTCTTGCTCTTCGTCATTTTGCTGGCGACCCTTTTCGGTCAAAACATCCTGCTGCTTTTCGTGCCGCTAGGCATGGTTTCctgg                                 >  2:2598215/1‑93 (MQ=255)

                                                        tCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCctgg                                 >  2:241189/1‑93 (MQ=255)

                                                         ccTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCctggc                                >  1:468238/1‑93 (MQ=255)

                                                         ccTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCctggc                                >  1:1214980/1‑93 (MQ=255)

                                                         ccTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCctggc                                <  2:1950716/93‑1 (MQ=255)

                                                         ccTTCCCATTCATGTTCTTCGTCATTTTGCCGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCctggc                                <  1:3598057/93‑1 (MQ=255)

                                                          cTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCctggct                               >  1:4205216/1‑93 (MQ=255)

                                                             cccATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGa                            <  2:3432287/93‑1 (MQ=255)

                                                             cccATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGa                            <  1:556017/93‑1 (MQ=255)

                                                               cATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGata                          >  1:2800830/1‑93 (MQ=255)

                                                               cATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGata                          >  1:3235147/1‑93 (MQ=255)

                                                               cATTCATGTTCTTCGTCATTTTGCAGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCACGGTTTCCTGGCTGGata                          <  1:2659855/93‑1 (MQ=255)

                                                                aTTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGatat                         >  1:2626076/1‑93 (MQ=255)

                                                                aTTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGatat                         <  1:892651/93‑1 (MQ=255)

                                                                 ttCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATg                        >  1:1130980/1‑93 (MQ=255)

                                                                   cATGTTCTTCGTCATTTTGCTGGCGATCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGc                      <  2:2921111/93‑1 (MQ=255)

                                                                   cATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGc                      >  2:1498990/1‑93 (MQ=255)

                                                                   cATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGATTTCCTGGCTGGATATGGc                      <  2:4553908/93‑1 (MQ=255)

                                                                    aTGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATggtc                     >  2:1216183/1‑91 (MQ=255)

                                                                    aTGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCt                     <  2:1166303/93‑1 (MQ=255)

                                                                     tGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTc                    >  1:4644433/1‑93 (MQ=255)

                                                                     tGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTc                    >  1:704728/1‑93 (MQ=255)

                                                                      gTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCg                   >  1:3221151/1‑93 (MQ=255)

                                                                        tcttcGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCgct                 >  2:4318180/1‑91 (MQ=255)

                                                                        tcttcGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGtt                 >  1:4548147/1‑92 (MQ=255)

                                                                        tcttcGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTa                 <  2:1553936/93‑1 (MQ=255)

                                                                        tcttcGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTa                 <  1:4269398/93‑1 (MQ=255)

                                                                         cttcGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTccgat                >  2:4093337/1‑89 (MQ=255)

                                                                          ttcGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTAtt               <  2:3993944/93‑1 (MQ=255)

                                                                           tcGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTAttt              >  2:3241250/1‑92 (MQ=255)

                                                                            cGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATtgt             <  1:1089639/93‑1 (MQ=255)

                                                                             gTCATTTTGCTGGCGACCTTTTTTGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGGTTCCTGGCTGGATATGGCTCGTTTtttg            >  2:748/1‑93 (MQ=255)

                                                                             gTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATtttg            >  1:4356425/1‑93 (MQ=255)

                                                                              tCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGCATtgtgg           >  1:1214752/1‑92 (MQ=255)

                                                                              tCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCCTAtttttg           >  2:1314076/1‑89 (MQ=255)

                                                                                 ttttGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTg        <  1:375303/93‑1 (MQ=255)

                                                                                   ttGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTggg      <  1:3633185/93‑1 (MQ=255)

                                                                                      cTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGCATTTTGCGTggggca   >  1:1563409/1‑90 (MQ=255)

                                                                                       tGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATAGCTCGTATTGTGCGTGGGCaaa  >  1:2390139/1‑93 (MQ=255)

GTTATCTGGGCGGTAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGTGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAA  >  NC_000913/1302237‑1302416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`