BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,330,421	A→G	L450L (TTA→TTG)	topA →	DNA topoisomerase type I omega protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,330,421	0	A	G	100.0%	391.1 / NA	122	L450L (TTA→TTG)	topA	DNA topoisomerase type I omega protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (64/58); total (64/58)

Only 100 of 126 total aligned reads displayed.

CCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGC  >  NC_000913/1330331‑1330510

ccGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGcc                                                                                         <  1:68087/93‑1 (MQ=255)

 cGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCca                                                                                        <  2:1435359/93‑1 (MQ=255)

  gCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCcag                                                                                       <  1:3932911/93‑1 (MQ=255)

   ccTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCcagc                                                                                      >  2:3565406/1‑93 (MQ=255)

   ccTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCcagc                                                                                      <  1:214725/93‑1 (MQ=255)

    cTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCcagca                                                                                     <  1:1816123/93‑1 (MQ=255)

    cTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCcagca                                                                                     <  2:2167966/93‑1 (MQ=255)

     tGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCcagcag                                                                                    >  1:103718/1‑93 (MQ=255)

       aaaGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGtt                                                                                  <  2:334107/93‑1 (MQ=255)

        aaGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTta                                                                                 <  1:1307610/93‑1 (MQ=255)

         aGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTtaa                                                                                >  1:1421930/1‑93 (MQ=255)

           cACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTtaata                                                                              <  2:224899/93‑1 (MQ=255)

            aCGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTtaataa                                                                             >  2:3667139/1‑93 (MQ=255)

             cgcgGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATaaa                                                                            <  1:144351/93‑1 (MQ=255)

             cgcgGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATaaa                                                                            <  1:684222/93‑1 (MQ=255)

              gcgGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAg                                                                           >  1:2157379/1‑93 (MQ=255)

                ggTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGc                                                                         <  1:1554910/93‑1 (MQ=255)

                 gTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCg                                                                        >  1:647631/1‑93 (MQ=255)

                 gTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCg                                                                        >  2:1958873/1‑93 (MQ=255)

                  tCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGa                                                                       >  1:1703045/1‑93 (MQ=255)

                  tCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGa                                                                       <  1:2641843/93‑1 (MQ=255)

                  tCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGa                                                                       <  2:1387334/93‑1 (MQ=255)

                  tCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGa                                                                       >  2:544826/1‑93 (MQ=255)

                  tCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGa                                                                       >  1:3422854/1‑93 (MQ=255)

                  tCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGa                                                                       <  1:3358191/93‑1 (MQ=255)

                  tCGTATTTTGCGTTTTGATGGCTGCACAAAAGTGATGCCTGCGTTGCGTAAACGCGATGACGATCACATCTTGCCAGCAGTTAATAAAGGCGa                                                                       <  2:2466613/93‑1 (MQ=255)

                     tATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGc                                                                    <  2:3612540/93‑1 (MQ=255)

                     tATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGc                                                                    <  2:2039163/93‑1 (MQ=255)

                     tATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGc                                                                    <  2:4318155/93‑1 (MQ=255)

                     tATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGc                                                                    >  1:3232404/1‑93 (MQ=255)

                      aTTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGct                                                                   >  1:2916675/1‑93 (MQ=255)

                       ttttGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGctc                                                                  >  2:3993143/1‑93 (MQ=255)

                       ttttGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGctc                                                                  >  1:4171319/1‑93 (MQ=255)

                        tttGCTTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGGTCGCATCTTGCCAGCAGTTAATAAAGGCGATGctct                                                                 <  2:3192115/93‑1 (MQ=255)

                          tGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGa                                                               <  2:3359259/93‑1 (MQ=255)

                           gCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGAc                                                              <  2:2155491/93‑1 (MQ=255)

                             gTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGc                                                            >  1:3898961/1‑93 (MQ=255)

                             gTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGc                                                            >  1:4322501/1‑93 (MQ=255)

                              ttttGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGTTCGCATCTTGCCAGAACTTAATAAAGGCGATGCTCTGACGCt                                                           >  1:4615247/1‑93 (MQ=255)

                              ttttGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCt                                                           >  1:3143781/1‑93 (MQ=255)

                                  gATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGtt                                                       <  2:872552/93‑1 (MQ=255)

                                  gATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGtt                                                       >  2:3873935/1‑93 (MQ=255)

                                    tGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGa                                                     <  2:1868129/93‑1 (MQ=255)

                                    tGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGa                                                     <  2:3148381/93‑1 (MQ=255)

                                    tGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGa                                                     >  1:3193072/1‑93 (MQ=255)

                                     ggCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGaa                                                    >  2:1523249/1‑93 (MQ=255)

                                      gCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAAc                                                   <  2:3651071/93‑1 (MQ=255)

                                       cTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACt                                                  <  1:4005054/93‑1 (MQ=255)

                                        ggaaCAAAATTGATCACTGCGTTGCCTAAAGGCGATGAATATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAGCtt                                                 <  2:1658804/90‑1 (MQ=255)

                                          gACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTac                                               >  2:4235322/1‑93 (MQ=255)

                                          gACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTac                                               <  2:935343/93‑1 (MQ=255)

                                             aaaaGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACAcc                                            >  1:4259710/1‑93 (MQ=255)

                                             aaaaGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACAcc                                            <  2:3946192/93‑1 (MQ=255)

                                             aaaaGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACAcc                                            <  2:4427533/93‑1 (MQ=255)

                                               aaGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACAccag                                          <  2:1322277/93‑1 (MQ=255)

                                                 gTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACAccagcc                                        >  1:3346110/1‑93 (MQ=255)

                                                    aTGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACAccagcccag                                     >  2:1754454/1‑93 (MQ=255)

                                                     tGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGGAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACAccagcccagc                                    >  1:2512974/1‑93 (MQ=255)

                                                        cTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACt                                 >  2:1264787/1‑93 (MQ=255)

                                                         tgcgttgcgtAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACtt                                >  2:4314089/1‑93 (MQ=255)

                                                         tgcgttgcgtAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACtt                                <  1:143807/93‑1 (MQ=255)

                                                          gcgttgcgtAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACttt                               <  2:3345575/93‑1 (MQ=255)

                                                           cgttgcgtAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTa                              <  2:2205152/93‑1 (MQ=255)

                                                            gttgcgtAAAGGCGATGAAGATCGCTTCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTAc                             <  1:4528822/93‑1 (MQ=255)

                                                            gttgcgtAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTAc                             >  2:2562470/1‑93 (MQ=255)

                                                             ttgcgtAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTAcc                            <  2:2641073/93‑1 (MQ=255)

                                                               gcgtAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCaa                          <  1:1681066/93‑1 (MQ=255)

                                                               gcgtAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCaa                          >  2:4111747/1‑93 (MQ=255)

                                                                cgtAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAg                         >  2:4529768/1‑93 (MQ=255)

                                                                 gtAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAgc                        >  1:2438586/1‑93 (MQ=255)

                                                                  tAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAgcc                       >  1:3368649/1‑93 (MQ=255)

                                                                   aaaGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAgccg                      >  1:2399273/1‑93 (MQ=255)

                                                                     aGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGACCAGCACTTTACCAAgccgcc                    >  1:962589/1‑93 (MQ=255)

                                                                      ggCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCa                   >  1:1135383/1‑93 (MQ=255)

                                                                      ggCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCa                   <  1:726066/93‑1 (MQ=255)

                                                                       gCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAg                  <  2:904034/93‑1 (MQ=255)

                                                                         gATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGcc                >  2:3896540/1‑93 (MQ=255)

                                                                          aTGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCAGTTTACCAAGCCGCCAGccc               >  1:4419756/1‑93 (MQ=255)

                                                                          aTGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGccc               >  1:3259934/1‑93 (MQ=255)

                                                                          aTGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGccc               >  2:639956/1‑93 (MQ=255)

                                                                           tGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCg              >  1:593103/1‑93 (MQ=255)

                                                                             aaGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGtt            <  1:1891585/93‑1 (MQ=255)

                                                                             aaGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGtt            >  1:2212542/1‑93 (MQ=255)

                                                                             aaGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGtt            <  1:30603/93‑1 (MQ=255)

                                                                             aaGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGtt            >  2:3975702/1‑93 (MQ=255)

                                                                              aGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGttt           >  2:1681314/1‑93 (MQ=255)

                                                                               gATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTc          <  1:691079/93‑1 (MQ=255)

                                                                                 tCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCACGTTTCAg        >  1:380450/1‑93 (MQ=255)

                                                                                  cGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGt       <  1:1673982/93‑1 (MQ=255)

                                                                                  cGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGt       <  1:4513281/93‑1 (MQ=255)

                                                                                    cATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGa     <  2:3967643/93‑1 (MQ=255)

                                                                                     aTCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGaa    <  2:975197/93‑1 (MQ=255)

                                                                                     aTCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGaa    <  2:2372045/93‑1 (MQ=255)

                                                                                     aTCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGaa    >  2:2279872/1‑93 (MQ=255)

                                                                                     aTCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGaa    >  1:1014106/1‑93 (MQ=255)

                                                                                     aTCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGaa    >  2:1782768/1‑93 (MQ=255)

                                                                                      tCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCCGTGAAg   >  2:186106/1‑93 (MQ=255)

                                                                                      tCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAg   >  1:288220/1‑93 (MQ=255)

                                                                                      tCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAg   <  1:374984/93‑1 (MQ=255)

                                                                                       cTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGc  <  2:4171319/93‑1 (MQ=255)

CCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGC  >  NC_000913/1330331‑1330510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`