BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,341,199	T→C	S52G (AGT→GGT)	osmB ←	osmotically inducible lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,341,199	0	T	C	100.0%	306.1 / NA	98	S52G (AGT→GGT)	osmB	osmotically inducible lipoprotein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (63/35); total (63/35)

Only 100 of 103 total aligned reads displayed.

CGATAATCGACATTTATTTACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCA  >  NC_000913/1341111‑1341287

cGATAATCGACATTTATTTACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCa                                                                                      <  1:1090548/93‑1 (MQ=255)

cGATAATCGACATTTATTTACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCa                                                                                      >  2:2211154/1‑93 (MQ=255)

cGATAATCGACATTTATTTACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCa                                                                                      <  2:645940/93‑1 (MQ=255)

cGATAATCGACATTTATTTACGATTATTTACCGACCTGGTGACAAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCa                                                                                      <  1:971584/93‑1 (MQ=255)

 gATAATCGACATTTATTTACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCAt                                                                                     >  1:3460461/1‑93 (MQ=255)

 gATAATCGACATTTATTTACGATTATTTACCGACCTGGTGACCAATAAAACCTCCGACAGCTGAACCACCTAATGTACCCACCGTACCGCCAt                                                                                     >  2:3085125/1‑93 (MQ=255)

  aTAATCGACATTTATTTACGATTATTTACCGTCCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATc                                                                                    <  2:3761844/93‑1 (MQ=255)

  aTAATCGACATTTATTTACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATc                                                                                    >  1:2728936/1‑93 (MQ=255)

   tAATCGACATTTATTTACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCg                                                                                   >  2:4075040/1‑93 (MQ=255)

    aaTCGACATTTATTTACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCgg                                                                                  <  1:110542/93‑1 (MQ=255)

     aTCGACATTTATTTACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGt                                                                                 >  1:4320261/1‑93 (MQ=255)

        gACATTTATTTACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAg                                                                              <  2:2066365/93‑1 (MQ=255)

        gACATTTATTTACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAg                                                                              >  1:4683960/1‑93 (MQ=255)

         aCATTTATTTACGATTATTTACCGACCTGGTGACCAATACCACCTCCGACAGCTGCACCACCTAATGTATCCAACGTACCGCCATCGGTCAGt                                                                             >  1:256254/1‑93 (MQ=255)

         aCATTTATTTACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGt                                                                             >  1:2358977/1‑93 (MQ=255)

         aCATTTATTTACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGt                                                                             >  1:3343690/1‑93 (MQ=255)

          cATTTATTTACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTa                                                                            >  2:3775879/1‑93 (MQ=255)

          cATTTATTTACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTa                                                                            >  1:208269/1‑93 (MQ=255)

            tttatttaCGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACt                                                                          >  1:846133/1‑93 (MQ=255)

             ttatttaCGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATATACCCCCCGTACCGCCATCGGTCCGTACtc                                                                         >  2:1385801/1‑92 (MQ=255)

              tatttaCGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGc                                                                        >  2:1237049/1‑93 (MQ=255)

               atttaCGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCa                                                                       >  1:1421747/1‑93 (MQ=255)

               atttaCGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCa                                                                       >  2:3095972/1‑93 (MQ=255)

                tttaCGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCAc                                                                      >  1:1239069/1‑93 (MQ=255)

                tttaCGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCAc                                                                      >  2:4438250/1‑93 (MQ=255)

                 ttaCGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCAcc                                                                     >  2:3689195/1‑93 (MQ=255)

                 ttaCGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCAcc                                                                     >  1:4506848/1‑93 (MQ=255)

                   aCGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGc                                                                   >  1:1758115/1‑93 (MQ=255)

                     gATTATTTACCGACCTGGTGACCAATAGCACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCt                                                                 >  2:2364767/1‑93 (MQ=255)

                     gATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCt                                                                 >  2:2892275/1‑93 (MQ=255)

                     gATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCt                                                                 <  2:3042078/93‑1 (MQ=255)

                       ttattTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTaa                                                               >  1:2169225/1‑93 (MQ=255)

                        tattTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAAt                                                              <  2:4235089/93‑1 (MQ=255)

                         attTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATg                                                             <  2:3275548/93‑1 (MQ=255)

                         attTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATg                                                             >  1:541511/1‑93 (MQ=255)

                          tttACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGc                                                            >  2:4391882/1‑93 (MQ=255)

                            tACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGAACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGccc                                                          <  1:300718/93‑1 (MQ=255)

                             aCCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCTATCGGTCAGTACTGCACCGCCTAATGcccc                                                         <  2:1303496/93‑1 (MQ=255)

                              ccGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCt                                                        <  2:1117682/93‑1 (MQ=255)

                                gACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGc                                                      >  1:576539/1‑93 (MQ=255)

                                 aCCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCa                                                     >  1:1391395/1‑93 (MQ=255)

                                 aCCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCa                                                     >  1:1466396/1‑93 (MQ=255)

                                 aCCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCa                                                     >  1:3263938/1‑93 (MQ=255)

                                 aCCTGGGGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCACCTGCa                                                     >  2:111429/1‑93 (MQ=255)

                                  ccTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCAc                                                    >  2:2250001/1‑93 (MQ=255)

                                   cTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCAcc                                                   >  1:2533483/1‑93 (MQ=255)

                                   cTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCAcc                                                   >  1:2512296/1‑93 (MQ=255)

                                    tGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCAccc                                                  >  2:2077310/1‑93 (MQ=255)

                                     ggTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCcg                                                 >  1:2784006/1‑93 (MQ=255)

                                        gACCAATAACACCTCCGACAGCTCCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCgcgc                                              >  1:822853/1‑93 (MQ=255)

                                         aCCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGcc                                             <  2:425076/93‑1 (MQ=255)

                                         aCCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGcc                                             >  1:3497221/1‑93 (MQ=255)

                                         aCCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGcc                                             >  2:3960160/1‑93 (MQ=255)

                                          ccAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCg                                            >  2:3951811/1‑93 (MQ=255)

                                          ccAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCg                                            >  2:2688598/1‑93 (MQ=255)

                                          ccAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCg                                            >  2:4026266/1‑93 (MQ=255)

                                           cAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGa                                           >  1:1805373/1‑93 (MQ=255)

                                           cAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGa                                           <  2:2175616/93‑1 (MQ=255)

                                            aaTAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGACGAt                                          >  2:2293077/1‑93 (MQ=255)

                                             aTAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGAtt                                         <  2:2464152/93‑1 (MQ=255)

                                             aTAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGAtt                                         <  1:3906932/93‑1 (MQ=255)

                                              tAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTg                                        <  1:3576672/93‑1 (MQ=255)

                                              tAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTg                                        >  2:3400423/1‑93 (MQ=255)

                                                 cacCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCgg                                     <  2:651050/93‑1 (MQ=255)

                                                  cccTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGgt                                    <  1:3466140/92‑1 (MQ=255)

                                                  acCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGgt                                    >  1:4292998/1‑93 (MQ=255)

                                                  acCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGgt                                    <  2:3307498/93‑1 (MQ=255)

                                                   ccTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGgtg                                   <  2:1505896/93‑1 (MQ=255)

                                                   ccTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGgtg                                   >  2:4192263/1‑93 (MQ=255)

                                                   ccTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGgtg                                   >  1:1085221/1‑93 (MQ=255)

                                                    cTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGgtgt                                  >  2:1352005/1‑93 (MQ=255)

                                                     tCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGtt                                 <  2:2469492/93‑1 (MQ=255)

                                                      ccGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTg                                <  1:3654644/93‑1 (MQ=255)

                                                        gACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCg                              >  2:2782130/1‑93 (MQ=255)

                                                        gACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCg                              >  2:1084452/1‑93 (MQ=255)

                                                         aCAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCgg                             >  1:1245059/1‑93 (MQ=255)

                                                          cAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGt                            >  2:1581094/1‑93 (MQ=255)

                                                                 accaccTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGttt                     >  1:3015481/1‑93 (MQ=255)

                                                                  ccaccTAATGTTCCCAACGTCCCGCCATCGCTCAGTCCTGCCCCGCCTAAGGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTa                    <  2:2347608/93‑1 (MQ=255)

                                                                  ccaccTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTa                    >  2:3919451/1‑93 (MQ=255)

                                                                   caccTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAg                   <  1:1583081/93‑1 (MQ=255)

                                                                     ccTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGAc                 <  2:4065302/93‑1 (MQ=255)

                                                                     ccTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGAc                 <  2:4323039/93‑1 (MQ=255)

                                                                      cTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGAcc                >  2:1785518/1‑93 (MQ=255)

                                                                      cTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGAcc                >  2:4226856/1‑93 (MQ=255)

                                                                       tAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCa               >  1:2825835/1‑93 (MQ=255)

                                                                        aaTGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAg              <  2:1483225/93‑1 (MQ=255)

                                                                          tGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGtt            <  1:2908703/93‑1 (MQ=255)

                                                                          tGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGtt            <  2:443184/93‑1 (MQ=255)

                                                                             aCCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGa         <  2:2778368/93‑1 (MQ=255)

                                                                             aCCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGa         >  1:1680813/1‑93 (MQ=255)

                                                                              cccAACGTACCGCCATCGGTCAGTACTGCACCGCGTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCTGTTAGaa        <  2:4453570/93‑1 (MQ=255)

                                                                              cccAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGaa        >  2:1939359/1‑93 (MQ=255)

                                                                              cccAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGaa        <  1:1945659/93‑1 (MQ=255)

                                                                               ccAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAAc       >  2:53552/1‑93 (MQ=255)

                                                                                cAACGTACCGCCATCGGTCAGTACTGCTCCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACa      <  1:4608711/93‑1 (MQ=255)

                                                                                cAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCCCCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACa      <  2:1421684/93‑1 (MQ=255)

                                                                                 aaCGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAg     >  2:364105/1‑93 (MQ=255)

                                                                                  aCGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAgg    >  2:3386886/1‑93 (MQ=255)

                                                                                    gTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCa  >  1:704038/1‑93 (MQ=255)

CGATAATCGACATTTATTTACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCA  >  NC_000913/1341111‑1341287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`