BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	122,235	T→C	R48R (CGT→CGC)	pdhR →	transcriptional regulator for pyruvate dehydrogenase complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	122,235	0	T	C	100.0%	532.8 / NA	169	R48R (CGT→CGC)	pdhR	transcriptional regulator for pyruvate dehydrogenase complex
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (90/79); total (90/79)

Only 100 of 181 total aligned reads displayed.

ACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGTCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCT  >  NC_000913/122145‑122324

aCTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGccc                                                                                         >  1:796650/1‑93 (MQ=255)

aCTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGccc                                                                                         <  2:4084970/93‑1 (MQ=255)

aCTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGccc                                                                                         >  1:3460144/1‑93 (MQ=255)

  tGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCcct                                                                                       >  2:83970/1‑93 (MQ=255)

  tGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCcct                                                                                       >  1:169114/1‑93 (MQ=255)

  tGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCcct                                                                                       >  2:676387/1‑93 (MQ=255)

   ggAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCcctc                                                                                      <  2:3390362/93‑1 (MQ=255)

    gAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGCCTCCCGCCcctct                                                                                     >  2:1511816/1‑92 (MQ=255)

     aGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCcctcct                                                                                    <  1:2098603/93‑1 (MQ=255)

      gTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAAGGCGAACTGGCAAACCAGTTTGACATCTCCCGCCcctcctg                                                                                   >  1:4143212/1‑92 (MQ=255)

       tttttGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGCCTCCCGCCCCTCCTTg                                                                                  >  2:1288397/1‑93 (MQ=255)

         tttGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCg                                                                                <  1:1481442/93‑1 (MQ=255)

          ttGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGt                                                                               <  1:271682/93‑1 (MQ=255)

            gATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGa                                                                             >  1:1652773/1‑93 (MQ=255)

            gATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGa                                                                             >  1:1805287/1‑93 (MQ=255)

             aTCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAg                                                                            <  2:1336754/93‑1 (MQ=255)

             aTCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAg                                                                            >  2:387570/1‑93 (MQ=255)

               ccTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAggg                                                                          >  2:2363969/1‑92 (MQ=255)

               ccTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGc                                                                          >  2:3494647/1‑93 (MQ=255)

                cTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCg                                                                         >  2:2512892/1‑93 (MQ=255)

                cTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCg                                                                         >  1:1467388/1‑93 (MQ=255)

                 tCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGa                                                                        >  2:2382108/1‑93 (MQ=255)

                   gAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGAtt                                                                      >  2:3607186/1‑93 (MQ=255)

                    aaGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTc                                                                     >  2:4494570/1‑93 (MQ=255)

                      ggCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCaa                                                                   <  1:2673490/93‑1 (MQ=255)

                         cctctCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGt                                                                <  1:1528543/92‑1 (MQ=255)

                          ctctCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGtc                                                               >  2:3904387/1‑93 (MQ=255)

                          ctctCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGtc                                                               <  1:3241636/93‑1 (MQ=255)

                           tctcCGCCCGGGCGAAAACCGCCCACCGGAACGCGATCTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGtct                                                              <  2:920893/93‑1 (MQ=255)

                            ctcCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGtctc                                                             >  1:1026550/1‑93 (MQ=255)

                            ctcCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGtctc                                                             <  1:1624891/93‑1 (MQ=255)

                            ctcCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGtctc                                                             <  1:1991485/93‑1 (MQ=255)

                            ctcCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGtctc                                                             >  1:1386898/1‑93 (MQ=255)

                               cGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTcgaa                                                          <  1:3568691/93‑1 (MQ=255)

                               cGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTcgaa                                                          <  1:616740/93‑1 (MQ=255)

                               cGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTcgaa                                                          <  1:2249169/93‑1 (MQ=255)

                               cGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTcgaa                                                          <  2:4129302/93‑1 (MQ=255)

                                 cccGGGCGAAAAACTCCCACCGGAACGCGAACTTGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTcgaagc                                                        >  1:1242808/1‑93 (MQ=255)

                                 cccGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTcgaagc                                                        <  1:3683193/93‑1 (MQ=255)

                                   cGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTcgaagcga                                                      >  2:2465479/1‑93 (MQ=255)

                                    gggCGAAAAACTCCCACCGGAACGCGACCTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTcgaagcgaa                                                     <  2:3084296/93‑1 (MQ=255)

                                     ggCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTcgaagcgaag                                                    <  1:1768580/93‑1 (MQ=255)

                                     gccGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTcgaagcgaag                                                    <  1:4020547/91‑1 (MQ=255)

                                      gCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAgg                                                   >  2:2174519/1‑93 (MQ=255)

                                      gCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAgg                                                   >  2:1709555/1‑93 (MQ=255)

                                        gAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGc                                                 <  1:3889949/93‑1 (MQ=255)

                                         aaaacCTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCt                                                <  2:2698084/93‑1 (MQ=255)

                                          aaaaCTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCtt                                               >  2:1326972/1‑93 (MQ=255)

                                           aaaCTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCttg                                              >  2:1126927/1‑93 (MQ=255)

                                            aaCTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCttgt                                             >  1:403650/1‑93 (MQ=255)

                                              cTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCttgttg                                           >  1:3789959/1‑93 (MQ=255)

                                               tCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGc                                          >  1:2222453/1‑93 (MQ=255)

                                                 ccACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCtt                                        <  1:3098628/93‑1 (MQ=255)

                                                 ccACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCtt                                        <  2:626575/93‑1 (MQ=255)

                                                   aCCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGCAGGGGTTGTTGCTtct                                      >  2:816742/1‑92 (MQ=255)

                                                   aCCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTtcg                                      <  1:433571/93‑1 (MQ=255)

                                                   aCCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTtcg                                      <  1:2439558/93‑1 (MQ=255)

                                                     cGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTtcgtc                                    <  2:3250670/93‑1 (MQ=255)

                                                     cGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTtcgtc                                    >  1:3854894/1‑93 (MQ=255)

                                                       gAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGc                                  <  1:2820709/93‑1 (MQ=255)

                                                         aCGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCa                                >  1:1316824/1‑93 (MQ=255)

                                                         aCGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCa                                >  2:696810/1‑93 (MQ=255)

                                                         aCGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCa                                >  2:4218817/1‑93 (MQ=255)

                                                           gcgAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAgg                              <  1:942659/93‑1 (MQ=255)

                                                             taaCTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGt                            <  1:733928/92‑1 (MQ=255)

                                                             gAACTGGCAAACCAGTTTGCCGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGt                            <  1:4110417/93‑1 (MQ=255)

                                                             gAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGCGAGGCGATTCAACGTCTCGAAGCGAAATGCTTGTTGCTTCGTCGCCAgggc                            >  2:965864/1‑92 (MQ=255)

                                                              aaCTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTg                           >  1:19523/1‑93 (MQ=255)

                                                              aaCTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTg                           <  1:462085/93‑1 (MQ=255)

                                                                cTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTggc                         >  1:2726099/1‑93 (MQ=255)

                                                                cTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTggc                         <  1:46011/93‑1 (MQ=255)

                                                                 tGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTggcg                        <  1:2163138/93‑1 (MQ=255)

                                                                 tGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTggcg                        >  1:2638367/1‑93 (MQ=255)

                                                                  ggCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTggcgg                       <  1:1962386/93‑1 (MQ=255)

                                                                   gCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTggcggc                      >  2:3404549/1‑93 (MQ=255)

                                                                   gCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTggcggc                      >  1:4658888/1‑93 (MQ=255)

                                                                    cAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCa                     <  1:1039655/93‑1 (MQ=255)

                                                                    aaaaaCAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCa                     <  2:4395645/92‑1 (MQ=255)

                                                                        aCAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACttt                 >  2:2271153/1‑93 (MQ=255)

                                                                        aCAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACttt                 >  2:2949140/1‑93 (MQ=255)

                                                                         cAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACtttt                <  1:1267691/93‑1 (MQ=255)

                                                                         aaGTTTGTGGTCTCCCGCCCCTCCTTGCGTGAGCCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACtttt                <  2:1073742/92‑1 (MQ=255)

                                                                          aGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTg               >  1:878244/1‑93 (MQ=255)

                                                                          aGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTg               >  2:1164472/1‑93 (MQ=255)

                                                                          aGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTg               >  1:3707453/1‑93 (MQ=255)

                                                                          aGTGTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTg               <  1:738049/93‑1 (MQ=255)

                                                                           gTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGt              >  1:3202501/1‑93 (MQ=255)

                                                                           gTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGt              >  1:645043/1‑93 (MQ=255)

                                                                            tttGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGAGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTc             <  1:2011797/93‑1 (MQ=255)

                                                                              tGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCa           >  1:1643894/1‑93 (MQ=255)

                                                                               gACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGCAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCag          >  1:4207833/1‑93 (MQ=255)

                                                                                aCGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCaga         >  2:3943198/1‑93 (MQ=255)

                                                                                aCGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCaga         <  2:1917338/93‑1 (MQ=255)

                                                                                   tctcCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGagca      <  2:2680764/93‑1 (MQ=255)

                                                                                    ctcCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGagcag     <  1:442823/93‑1 (MQ=255)

                                                                                     tcCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGagcagc    <  2:4061741/93‑1 (MQ=255)

                                                                                      cccGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGcc   >  1:2361985/1‑93 (MQ=255)

                                                                                      cccGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGcc   >  2:561482/1‑93 (MQ=255)

                                                                                       ccGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCt  <  2:441587/93‑1 (MQ=255)

                                                                                       ccGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCt  >  2:1516283/1‑93 (MQ=255)

ACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGTCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCT  >  NC_000913/122145‑122324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`