Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,403,088	T→C	S108S (AGT→AGC)	ydaK →	putative transcriptional regulator LYSR‑type

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,403,088	0	T	C	100.0%	233.0 / NA	75	S108S (AGT→AGC)	ydaK	putative transcriptional regulator LYSR‑type
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/39);  total (36/39)

TGCGCGCAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGTCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAGGGCAACTGGTGTCGATGATTAA  >  NC_000913/1403000‑1403177                                                                                         |                                                                                          tGCGCGCAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGa                                                                                       >  1:1387820/1‑93 (MQ=255)     cgcAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGcc                                                                                   >  1:334022/1‑93 (MQ=255)     cgcAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGcc                                                                                   >  2:4381664/1‑93 (MQ=255)      gcAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCa                                                                                  <  1:1935671/93‑1 (MQ=255)      gcAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCa                                                                                  <  2:1428289/93‑1 (MQ=255)      gcAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCa                                                                                  <  1:1619635/93‑1 (MQ=255)      gcAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCa                                                                                  <  2:4533679/93‑1 (MQ=255)       cAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAg                                                                                 <  1:1113141/93‑1 (MQ=255)        aGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGc                                                                                <  2:4606387/93‑1 (MQ=255)         gCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCt                                                                               <  2:3744701/93‑1 (MQ=255)           ccAAGAGGATATTCGCCAACGACAAGGGCAACTGGCAGGGCAGATTAATATCGGCCTGGCGGCCAGTATTTCACGCAGCCTGATGCCAGCTgc                                                                             >  1:3197127/1‑92 (MQ=255)           ccAAGAGGATATTCGCCAACGACAAGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGt                                                                             >  2:2827081/1‑93 (MQ=255)           ccAAGAGGATATTCGCCAACGACAAGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGt                                                                             >  1:212134/1‑93 (MQ=255)             aaGAGGATATTCGCCAACGACAAGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCa                                                                           <  1:402637/93‑1 (MQ=255)               gagGATATGCGCCAACGACAAGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCata                                                                         <  2:2974606/93‑1 (MQ=255)                agGATATTCGCCAACGACAAGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCatat                                                                        >  2:3853103/1‑93 (MQ=255)                 ggATATTCGCCAACGACAAGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATAtc                                                                       <  1:210019/93‑1 (MQ=255)                  gATATTCGCCAACGACAAGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATAtct                                                                      >  2:2368835/1‑93 (MQ=255)                  gATATTCGCCAACGACAAGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATAtct                                                                      >  2:523264/1‑93 (MQ=255)                   atatTCGCCAACGACAAGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATAtctc                                                                     >  2:4625432/1‑93 (MQ=255)                   atatTCGCCAACGACAAGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATAtctc                                                                     <  1:164419/93‑1 (MQ=255)                    tatTCGCCAACGACAAGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCg                                                                    >  1:4205926/1‑93 (MQ=255)                     atTCGCCACCGACAAGGGCAACTGGCAGGGCAGATTAATACCGGCATGGGGGCCAGTATGTCCCGCGGCCTGATGCCGGCTGTCATATCTCGt                                                                   <  2:4533163/93‑1 (MQ=255)                     atTCGCCAACGACAAGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGt                                                                   >  2:4214206/1‑93 (MQ=255)                           cAACGACAAGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTtcat                                                             >  2:771481/1‑93 (MQ=255)                              cGACAAGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATcag                                                          >  1:280601/1‑93 (MQ=255)                              cGACAAGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATcag                                                          <  2:3085100/93‑1 (MQ=255)                                aCAAGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATcagca                                                        >  2:3541069/1‑93 (MQ=255)                                  aaGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCagcagc                                                      >  2:4137103/1‑93 (MQ=255)                                   aGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAgcagca                                                     >  2:2505286/1‑93 (MQ=255)                                   aGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAgcagca                                                     <  1:124607/93‑1 (MQ=255)                                     ggCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATc                                                   <  2:3158244/93‑1 (MQ=255)                                     ggCAACTGCCAGGGCAGATTCATATCGGAATGGGGGCCAGTATTCCCCGCAGCCTGACGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATc                                                   <  2:1278656/93‑1 (MQ=255)                                      gCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATcc                                                  >  1:1648206/1‑93 (MQ=255)                                      gCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATcc                                                  <  1:1395050/93‑1 (MQ=255)                                        aaCTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGc                                                >  2:3190677/1‑93 (MQ=255)                                         aCTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCa                                               <  2:1670587/93‑1 (MQ=255)                                           tGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAgg                                             <  2:388029/93‑1 (MQ=255)                                           tGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAgg                                             <  2:2365213/93‑1 (MQ=255)                                            ggcagggcagATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGt                                            >  2:4078734/1‑93 (MQ=255)                                              cagggcagATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTaa                                          <  2:368742/93‑1 (MQ=255)                                               agggcagATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTaaa                                         >  1:1087056/1‑93 (MQ=255)                                                gggcagATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTaaaa                                        <  2:2285702/93‑1 (MQ=255)                                                gggcagATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTaaaa                                        >  2:1620353/1‑93 (MQ=255)                                                gggcagATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTaaaa                                        <  1:1538089/93‑1 (MQ=255)                                                 ggcagATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAg                                       <  2:1432104/93‑1 (MQ=255)                                                 ggcagATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTCATGCCAGCTGTCATATCTCGTTTTCATCAGCCGCATACGCAGGTAAAAg                                       >  2:4525342/1‑93 (MQ=255)                                                   cagATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTa                                     >  2:2345091/1‑93 (MQ=255)                                                   cagATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTa                                     <  1:3640750/93‑1 (MQ=255)                                                    agATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTAc                                    <  1:4208223/93‑1 (MQ=255)                                                         aaTATCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCAtt                               <  1:4501701/93‑1 (MQ=255)                                                          atatCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTa                              <  2:3401271/93‑1 (MQ=255)                                                           tatCGGCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGCAAAAGGACGCATTAt                             >  2:1290746/1‑93 (MQ=255)                                                                gCATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAg                        <  2:3202466/93‑1 (MQ=255)                                                                 cATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAgg                       <  1:3510022/93‑1 (MQ=255)                                                                 cATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAgg                       >  1:3206316/1‑93 (MQ=255)                                                                 cATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAgg                       >  1:1948554/1‑93 (MQ=255)                                                                 cATGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAgg                       <  2:2309993/93‑1 (MQ=255)                                                                  aTGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAggg                      >  1:3871592/1‑93 (MQ=255)                                                                  aTGGGGGCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAggg                      >  2:2700037/1‑93 (MQ=255)                                                                    gggggCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAGGGCa                    <  1:1223061/93‑1 (MQ=255)                                                                      gggCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAGGGCacc                  <  2:2958568/93‑3 (MQ=255)                                                                        gCCAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAGGGCAACTg                >  1:2360300/1‑93 (MQ=255)                                                                          cAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCCGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAGGCCAACTGgt              >  2:2340270/1‑93 (MQ=255)                                                                          cAGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAGGGCAACTGgt              <  2:1983829/93‑1 (MQ=255)                                                                           aGTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAGGGGAACTGgtg             >  2:357429/1‑93 (MQ=255)                                                                            gTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAGGGGAACTGgtgg            >  2:2431324/1‑92 (MQ=255)                                                                            gTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAGGGCAACTGgtgt            >  2:2770876/1‑93 (MQ=255)                                                                            gTATTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAGGGCAACTGgtgt            <  1:3935213/93‑1 (MQ=255)                                                                              aTTTCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAGGGCAACTGGTGTCg          <  1:3522548/93‑1 (MQ=255)                                                                                 tCCCGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAGGGCAACTGGTGTCgatg       >  1:1976464/1‑93 (MQ=255)                                                                                  cccGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAGGGCAACTGGTGTCgatga      <  2:3466470/93‑1 (MQ=255)                                                                                  cccGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAGGGCAACTGGTGTCgatga      <  1:2926679/93‑1 (MQ=255)                                                                                   ccGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAGGGCAACTGGTGTCgatgat     >  2:1445960/1‑93 (MQ=255)                                                                                   ccGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAGGGCAACTGGTGTCgatgat     >  1:1184541/1‑93 (MQ=255)                                                                                    cGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAGGGCAACTGGTGTCGATGAtt    >  1:1890678/1‑93 (MQ=255)                                                                                    cGCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAGGGCAACTGGTGTCGATGAtt    >  2:1051519/1‑93 (MQ=255)                                                                                     gCAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAGGGCAACTGGTGTCGATGATTa   <  1:2444094/93‑1 (MQ=255)                                                                                      cAGCCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAGGGCAACTGGTGTCGATGATTaa  <  1:3181696/93‑1 (MQ=255)                                                                                         |                                                                                          TGCGCGCAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGTCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAGGGCAACTGGTGTCGATGATTAA  >  NC_000913/1403000‑1403177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑