BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	127,038	G→A	A448A (GCG→GCA)	aceF →	pyruvate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	127,038	0	G	A	100.0%	658.2 / NA	181	A448A (GCG→GCA)	aceF	pyruvate dehydrogenase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (104/77); total (104/77)

Only 100 of 189 total aligned reads displayed.

TAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAA  >  NC_000913/126948‑127127

 aaCTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAgcagcg                                                                                        >  2:606140/1‑93 (MQ=255)

    tGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGTAGCGaaa                                                                                     <  1:3955650/93‑1 (MQ=255)

      ggTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACg                                                                                   <  1:1069271/93‑1 (MQ=255)

         aaTGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTaa                                                                                <  2:1005663/93‑1 (MQ=255)

          aTGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAg                                                                               >  2:4389126/1‑93 (MQ=255)

          aTGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAg                                                                               >  1:3741063/1‑93 (MQ=255)

          aTGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAg                                                                               <  1:3260218/93‑1 (MQ=255)

           tGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGc                                                                              >  2:4443449/1‑93 (MQ=255)

           tGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGc                                                                              >  2:2457209/1‑93 (MQ=255)

           tGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGc                                                                              >  2:1589922/1‑93 (MQ=255)

           tGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGc                                                                              >  1:4626714/1‑93 (MQ=255)

           tGACCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGc                                                                              <  1:447278/93‑1 (MQ=255)

            gATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCt                                                                             >  1:1580111/1‑93 (MQ=255)

             aTCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTg                                                                            >  1:971763/1‑93 (MQ=255)

                  gCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGAtgt                                                                       >  1:1070914/1‑93 (MQ=255)

                  gCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGAtgt                                                                       <  2:486346/93‑1 (MQ=255)

                   cATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGAtgtg                                                                      >  1:4669702/1‑93 (MQ=255)

                    aTGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGa                                                                     >  2:1247691/1‑93 (MQ=255)

                     tGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGaa                                                                    >  1:2441173/1‑93 (MQ=255)

                      gTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAg                                                                   >  1:904262/1‑93 (MQ=255)

                       ttACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGa                                                                  <  2:2087768/93‑1 (MQ=255)

                        tACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGAt                                                                 <  2:1117147/93‑1 (MQ=255)

                        tACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGAt                                                                 >  2:619201/1‑93 (MQ=255)

                        tACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGAt                                                                 >  2:461846/1‑93 (MQ=255)

                         aCTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGAta                                                                >  1:3635128/1‑92 (MQ=255)

                          cTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCa                                                               >  1:2490104/1‑93 (MQ=255)

                          cTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCa                                                               >  2:3765653/1‑93 (MQ=255)

                           tCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCAc                                                              >  2:324922/1‑93 (MQ=255)

                           tCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCAc                                                              <  2:3026677/93‑1 (MQ=255)

                             aCTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCAccc                                                            <  2:926113/93‑1 (MQ=255)

                             aCTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCAccc                                                            >  2:1476945/1‑93 (MQ=255)

                             aCTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCAccc                                                            <  2:2373502/93‑1 (MQ=255)

                              cTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCAcccc                                                           >  1:760477/1‑93 (MQ=255)

                               ttCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCg                                                          <  1:325474/93‑1 (MQ=255)

                                  gACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGtt                                                       <  2:2398042/93‑1 (MQ=255)

                                  gACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGtt                                                       >  2:1345494/1‑93 (MQ=255)

                                     aaaaCCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGCTGTc                                                    >  1:3978405/1‑93 (MQ=255)

                                      aaaCCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCt                                                   <  2:449685/93‑1 (MQ=255)

                                      aaaCCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCt                                                   >  1:2808238/1‑93 (MQ=255)

                                       aaCCGATGTCACCCGGGTGGAAGCCTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCtt                                                  <  1:3113080/93‑1 (MQ=255)

                                       aaCCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCtt                                                  >  2:2608970/1‑93 (MQ=255)

                                         ccGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTtca                                                <  1:3477896/93‑1 (MQ=255)

                                         ccGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTtca                                                >  1:602697/1‑93 (MQ=255)

                                          cGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTtcat                                               >  1:2471982/1‑93 (MQ=255)

                                           gATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTtcatc                                              <  2:1970443/93‑1 (MQ=255)

                                           gATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTtcatc                                              >  1:1805222/1‑93 (MQ=255)

                                            atatCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTtcatca                                             >  2:72299/1‑93 (MQ=255)

                                            atatCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTtcatca                                             >  1:1318087/1‑93 (MQ=255)

                                             tatCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTcatcat                                            >  2:3869556/1‑93 (MQ=255)

                                             tatCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTcatcat                                            <  2:2778263/93‑1 (MQ=255)

                                              atCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATg                                           >  1:2510022/1‑93 (MQ=255)

                                               acACCGACTTGGATGCGTTCCGTAAACAGCAGAAGGAAGAAGCTGCTATCCGTAGGCTGGATGTGAACATTGACCCGGTTGGCTACAACATGa                                          <  2:3540298/92‑1 (MQ=255)

                                                 aCCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGaaa                                        <  2:3820464/93‑1 (MQ=255)

                                                 aCCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGaaa                                        <  1:3083771/93‑1 (MQ=255)

                                                 aCCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGaaa                                        <  2:3214822/93‑1 (MQ=255)

                                                    gAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGcc                                     >  1:3280835/1‑93 (MQ=255)

                                                     aGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCg                                    <  1:1949940/93‑1 (MQ=255)

                                                      gTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGt                                   <  2:346366/93‑1 (MQ=255)

                                                       ttGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGtt                                  >  1:625218/1‑93 (MQ=255)

                                                       ttGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGtt                                  >  2:1977982/1‑93 (MQ=255)

                                                         tgAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTtgc                                <  1:1500874/92‑1 (MQ=255)

                                                         ggAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCCCCCCGGTTGTCTTCATCATGAAAGCCGTtgc                                <  1:2090823/93‑1 (MQ=255)

                                                          gAAGCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTtgct                               <  1:3674395/93‑1 (MQ=255)

                                                          gAAGCCTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTtgct                               <  1:2908560/93‑1 (MQ=255)

                                                             gCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCa                            >  1:2933154/1‑93 (MQ=255)

                                                             gCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCa                            >  2:4321126/1‑93 (MQ=255)

                                                             gCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCa                            <  2:3677968/93‑1 (MQ=255)

                                                             gCGTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCACCATGAGAGCAGTtgctgaa                            >  1:1200444/1‑91 (MQ=255)

                                                               gTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGc                          >  1:440370/1‑93 (MQ=255)

                                                               gTTCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGc                          >  2:3403322/1‑93 (MQ=255)

                                                                ttCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGct                         >  2:264481/1‑93 (MQ=255)

                                                                ttCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTAATCATGAAAGCAGTTGCTGCAGct                         >  2:746614/1‑93 (MQ=255)

                                                                 tCCGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGctc                        >  1:1885054/1‑93 (MQ=255)

                                                                   cGTAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCtt                      >  2:1122191/1‑93 (MQ=255)

                                                                     tAAACAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGa                    <  2:2924608/93‑1 (MQ=255)

                                                                      aaaCAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAg                   <  1:1683503/93‑1 (MQ=255)

                                                                       aaCAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGc                  >  2:885474/1‑93 (MQ=255)

                                                                       aaCAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGc                  >  1:1532315/1‑93 (MQ=255)

                                                                       aaCAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGc                  <  1:381692/93‑1 (MQ=255)

                                                                       aaCAGCAGAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGc                  >  2:4096416/1‑93 (MQ=255)

                                                                         cagcagAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAg                >  1:3012188/1‑93 (MQ=255)

                                                                           gcagAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGAt              >  1:4521665/1‑93 (MQ=255)

                                                                             agAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGc            >  1:4105859/1‑93 (MQ=255)

                                                                             agAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGc            <  2:3990225/93‑1 (MQ=255)

                                                                              gAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGcc           >  2:3916544/1‑93 (MQ=255)

                                                                              gAACGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGcc           <  2:1699723/93‑1 (MQ=255)

                                                                               aaCGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCt          >  2:2091844/1‑93 (MQ=255)

                                                                               aaCGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCt          >  1:2189473/1‑93 (MQ=255)

                                                                               aaCGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCt          <  1:2048774/93‑1 (MQ=255)

                                                                               aaCGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCt          >  1:643086/1‑93 (MQ=255)

                                                                                aCGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTc         >  2:4441164/1‑93 (MQ=255)

                                                                                aCGAAGAAGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTc         >  2:3304427/1‑93 (MQ=255)

                                                                                  gaagaaGCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGc       >  2:187418/1‑93 (MQ=255)

                                                                                   aagaagCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATCCCTCGCt      >  2:313749/1‑93 (MQ=255)

                                                                                    agaagCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCtt     >  1:553714/1‑93 (MQ=255)

                                                                                     gaagCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTc    <  1:2219912/93‑1 (MQ=255)

                                                                                     gaagCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTc    >  2:175371/1‑93 (MQ=255)

                                                                                     gaagCAGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTc    >  2:1904719/1‑93 (MQ=255)

                                                                                      gagcagcGAACCGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCa   <  1:2343164/92‑1 (MQ=255)

                                                                                       agcagcGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCaa  <  1:4536251/93‑1 (MQ=255)

TAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAA  >  NC_000913/126948‑127127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`