Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,419,658	C→T	P172P (CCC→CCT)	ydaU →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,419,658	0	C	T	100.0%	247.3 / NA	70	P172P (CCC→CCT)	ydaU	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (46/24);  total (46/24)

TGTTCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTG  >  NC_000913/1419568‑1419747                                                                                           |                                                                                          tgtTCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGAtcctcc                                                                                         <  2:2496689/93‑1 (MQ=255)  gtTCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATcctccc                                                                                        >  2:378932/1‑93 (MQ=255)  gtTCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATcctccc                                                                                        <  2:1110984/93‑1 (MQ=255)   ttCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATcctcccc                                                                                       >  1:1123492/1‑93 (MQ=255)   ttCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATcctcccc                                                                                       >  2:3143444/1‑93 (MQ=255)   ttCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATcctcccc                                                                                       >  1:1480053/1‑93 (MQ=255)   ttCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATcctcccc                                                                                       >  1:1847495/1‑93 (MQ=255)     cAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTa                                                                                     >  2:3932493/1‑93 (MQ=255)      aaaCAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTaa                                                                                    >  2:1104046/1‑93 (MQ=255)      aaaCAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTaa                                                                                    >  2:3177949/1‑93 (MQ=255)        aCAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAt                                                                                  >  2:1832589/1‑93 (MQ=255)          aGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATcc                                                                                <  1:171841/93‑1 (MQ=255)          aGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATcc                                                                                >  2:3627320/1‑93 (MQ=255)           gATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATccc                                                                               >  1:10179/1‑93 (MQ=255)             tGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATccccc                                                                             >  2:252941/1‑93 (MQ=255)              gTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATcccccc                                                                            <  1:2692389/93‑1 (MQ=255)               ttGAAGTGGTGTTTGAACATGATGTCAACACACAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATccccgcc                                                                           >  2:119159/1‑93 (MQ=255)                tGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAGCCCCCCCg                                                                          <  2:1251448/93‑1 (MQ=255)                 gAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCgg                                                                         >  2:268154/1‑93 (MQ=255)                 gAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCgg                                                                         >  2:182982/1‑93 (MQ=255)                 gAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCgg                                                                         <  2:3546686/93‑1 (MQ=255)                   aGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCgggg                                                                       <  2:4327865/93‑1 (MQ=255)                   aGTGGTGTGTGAACATAATGTCAACACAAAGGCAACTAATAAAGATACAGAAAAAGAACTAAAAACAGAACCCCCCCTAAAGCCCCCCCgggt                                                                       <  2:4379676/93‑2 (MQ=255)                           ttGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAg                                                               <  1:4515767/93‑1 (MQ=255)                             gAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGgt                                                             <  1:99747/93‑1 (MQ=255)                              aaCATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGgtg                                                            >  1:1697986/1‑93 (MQ=255)                               aCATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGgtgt                                                           >  1:3831653/1‑93 (MQ=255)                               aCATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGgtgt                                                           >  1:2960866/1‑93 (MQ=255)                                        cAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGtt                                                  <  1:3290340/93‑1 (MQ=255)                                        cAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGtt                                                  <  2:3233336/93‑1 (MQ=255)                                         aaCACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGttt                                                 >  1:931431/1‑93 (MQ=255)                                         aaCACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGttt                                                 <  1:1720153/93‑1 (MQ=255)                                         aaCACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGttt                                                 <  1:1311043/93‑1 (MQ=255)                                         aaCACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGttt                                                 >  1:4305864/1‑93 (MQ=255)                                         aaCACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGttt                                                 <  1:3729961/93‑1 (MQ=255)                                              aaaGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGAccc                                            >  2:4033633/1‑93 (MQ=255)                                                 ggCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCctct                                         >  1:4210303/1‑93 (MQ=255)                                                 ggCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCctct                                         >  1:4088125/1‑93 (MQ=255)                                                 ggCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCctct                                         <  1:2262541/93‑1 (MQ=255)                                                 agCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCctct                                         <  1:3370980/92‑1 (MQ=255)                                                      cTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCGAAAAGTTTGACCCTCTGGata                                    >  2:1099765/1‑93 (MQ=255)                                                      cTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGata                                    <  2:845026/93‑1 (MQ=255)                                                       taataaAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGatat                                   >  2:4109778/1‑93 (MQ=255)                                                       taataaAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGatat                                   >  1:3277227/1‑93 (MQ=255)                                                       taataaAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGatat                                   <  1:3972498/93‑1 (MQ=255)                                                       taataaAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGatat                                   >  1:4621363/1‑93 (MQ=255)                                                          taaAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTAc                                >  1:2019965/1‑93 (MQ=255)                                                            aaGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGCGTCAAAAAGTTTGACACTCTGGATATTACtt                              >  1:4251422/1‑93 (MQ=255)                                                             aGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACttt                             <  1:3706795/93‑1 (MQ=255)                                                              gATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTg                            >  2:1453235/1‑93 (MQ=255)                                                              gATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTg                            >  1:1432216/1‑93 (MQ=255)                                                              gATACAGATAAAGATCTAAAAACAGATCATCCCCTAAATCCCCCCCGGGGGAATCGAGGCGTCAAAAAGATTGACTCTCTGGATATTACTTTg                            >  2:1001690/1‑93 (MQ=255)                                                                tACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGcc                          >  2:1222715/1‑93 (MQ=255)                                                                tACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGGGTCAAAAAGTTTGACCCTCTGGATATTACTTTGcg                          >  1:591762/1‑92 (MQ=255)                                                                   aGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGaa                       >  2:338992/1‑93 (MQ=255)                                                                     aTAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACt                     <  2:21931/93‑1 (MQ=255)                                                                      tAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTg                    >  1:2425134/1‑93 (MQ=255)                                                                       aaaGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTgg                   >  1:1601427/1‑93 (MQ=255)                                                                        aaGATCTAAAAACAGACCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGa                  <  2:1903143/93‑1 (MQ=255)                                                                         aGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGAt                 >  1:1679925/1‑93 (MQ=255)                                                                         aGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGAt                 >  2:1385980/1‑93 (MQ=255)                                                                         aGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGAt                 >  2:3943655/1‑93 (MQ=255)                                                                          gATCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGAtt                >  1:1264493/1‑93 (MQ=255)                                                                            tCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTc              >  1:1216357/1‑93 (MQ=255)                                                                            tCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTc              >  2:4179303/1‑93 (MQ=255)                                                                            tCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTc              <  2:1518444/93‑1 (MQ=255)                                                                            tCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTc              <  2:3926266/93‑1 (MQ=255)                                                                            tCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTc              <  1:665069/93‑1 (MQ=255)                                                                            tCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTc              <  1:4532489/93‑1 (MQ=255)                                                                            tCTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGGGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTc              >  2:4146448/1‑93 (MQ=255)                                                                             cTAAAAACAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCt             <  2:4410086/93‑1 (MQ=255)                                                                                aaaaCAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGtc          >  1:4346662/1‑93 (MQ=255)                                                                                aaaaCAGAACCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGtc          <  1:1986895/93‑1 (MQ=255)                                                                                 aaaCAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGtct         >  2:2886096/1‑93 (MQ=255)                                                                                 aaaCAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGtct         >  2:829119/1‑93 (MQ=255)                                                                                  aaCAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGtctc        >  2:4029807/1‑93 (MQ=255)                                                                                   aCAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCg       >  2:2678429/1‑93 (MQ=255)                                                                                    cAGATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGc      >  2:819823/1‑93 (MQ=255)                                                                                      gATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCtt    >  2:180892/1‑93 (MQ=255)                                                                                      gATCCTCCCCTAAATCCCCCCCGGGGGAATCGAGCTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCtt    >  2:4209681/1‑93 (MQ=255)                                                                                        tcctccCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTg  <  2:2207850/93‑1 (MQ=255)                                                                                           |                                                                                          TGTTCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTG  >  NC_000913/1419568‑1419747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑