BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,455,493	T→C	V347A (GTG→GCG)	b1392 →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,455,493	0	T	C	96.0%	281.2 / 1.4	99	V347A (GTG→GCG)	b1392	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (3/1); new base C (48/47); total (51/48)
Fisher's exact test for biased strand distribution p-value = 6.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01

Only 100 of 105 total aligned reads displayed.

GATGGAAACCAATTACAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGC  >  NC_000913/1455404‑1455582

gATGGAAACCAATTACAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTggtggt                                                                                        <  1:2117666/93‑1 (MQ=255)

gATGGAAACCAATTACAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTggcggt                                                                                        >  2:4218737/1‑93 (MQ=255)

 aTGGAAACCAATTACAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGtt                                                                                       >  2:3526545/1‑93 (MQ=255)

    gAAACCAATTACAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGAc                                                                                    <  2:1199168/93‑1 (MQ=255)

    gAAACCAATTACAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGAc                                                                                    <  2:1612747/93‑1 (MQ=255)

     aaaCCAATTACAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACt                                                                                   >  1:4363670/1‑93 (MQ=255)

       aCCAATTACAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACttt                                                                                 <  2:3950150/93‑1 (MQ=255)

        ccAATTACAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTg                                                                                <  2:2716730/93‑1 (MQ=255)

          aaTTACAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGAc                                                                              <  1:3376464/93‑1 (MQ=255)

          aaTTACAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGAc                                                                              >  1:1434566/1‑93 (MQ=255)

          aaTTACAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGAc                                                                              <  1:2173910/93‑1 (MQ=255)

          aaTTACAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGAc                                                                              <  2:4122193/93‑1 (MQ=255)

               cAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGaa                                                                         <  1:4074166/93‑1 (MQ=255)

               cAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGaa                                                                         <  1:2654838/93‑1 (MQ=255)

                 gTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAgg                                                                       >  1:2189265/1‑93 (MQ=255)

                  tCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAggg                                                                      >  1:410874/1‑93 (MQ=255)

                    tGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGa                                                                    <  1:1594423/93‑1 (MQ=255)

                    tGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGa                                                                    >  1:265807/1‑93 (MQ=255)

                     ggAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGAt                                                                   >  2:4354035/1‑93 (MQ=255)

                     ggAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGCTGACTTTGACGCGAAGGGGag                                                                   >  2:2916206/1‑92 (MQ=255)

                       aaCCGGATGAACTGGCCGCAGGGTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATgg                                                                 >  1:4443203/1‑93 (MQ=255)

                        aCCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATggg                                                                >  2:2862556/1‑92 (MQ=255)

                        aCCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGc                                                                >  1:3522682/1‑93 (MQ=255)

                           ggATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGggatgggatg                                                             >  2:2509832/1‑93 (MQ=255)

                           ggATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATg                                                             <  2:2652360/93‑1 (MQ=255)

                           ggATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATg                                                             >  2:530015/1‑93 (MQ=255)

                            gATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGa                                                            >  1:2761411/1‑93 (MQ=255)

                            gATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGa                                                            <  1:698813/93‑1 (MQ=255)

                            gATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGa                                                            >  1:403963/1‑93 (MQ=255)

                            gATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGa                                                            <  1:4635571/93‑1 (MQ=255)

                            gATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGa                                                            >  1:1682805/1‑93 (MQ=255)

                             aTGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAg                                                           >  1:1401595/1‑93 (MQ=255)

                             aTGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAg                                                           <  2:250893/93‑1 (MQ=255)

                              tGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGc                                                          <  1:1426929/93‑1 (MQ=255)

                                aaCTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGGATGAGCGa                                                        >  2:2816384/1‑93 (MQ=255)

                                 aCTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGaa                                                       >  2:2626637/1‑93 (MQ=255)

                                 aCTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGaa                                                       <  2:1608028/93‑1 (MQ=255)

                                  cTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAAc                                                      <  1:2929330/93‑1 (MQ=255)

                                   tGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACt                                                     <  2:945421/93‑1 (MQ=255)

                                    ggCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTg                                                    <  2:4624721/93‑1 (MQ=255)

                                     gCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGa                                                   <  1:1622662/93‑1 (MQ=255)

                                      ccGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGAt                                                  <  2:1508584/93‑1 (MQ=255)

                                      ccGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGAt                                                  <  2:4279702/93‑1 (MQ=255)

                                       cGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATc                                                 <  2:1998092/93‑1 (MQ=255)

                                       cGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATc                                                 <  1:3657228/93‑1 (MQ=255)

                                       cGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATc                                                 >  1:592245/1‑93 (MQ=255)

                                       cGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATc                                                 <  2:1036516/93‑1 (MQ=255)

                                          aGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATcgtc                                              <  2:1038589/93‑1 (MQ=255)

                                            gTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATcgtcag                                            >  1:1699415/1‑93 (MQ=255)

                                            gTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGAATGAGCGAACTGATcgtcag                                            >  2:972995/1‑93 (MQ=255)

                                              tATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATcgtcagcc                                          >  2:2634763/1‑93 (MQ=255)

                                               aTGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATcgtcagccg                                         >  1:380353/1‑93 (MQ=255)

                                               aTGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATcgtcagccg                                         >  2:297756/1‑93 (MQ=255)

                                                tgtgTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATcgtcagccgt                                        <  1:1496151/93‑1 (MQ=255)

                                                  tgtTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATcgtcagccgtca                                      >  2:1589240/1‑93 (MQ=255)

                                                   gtTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATcgtcagccgtcag                                     <  2:2931153/93‑1 (MQ=255)

                                                   gtTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATcgtcagccgtcag                                     >  1:3696303/1‑93 (MQ=255)

                                                   gtTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATcgtcagccgtcag                                     >  1:3247513/1‑93 (MQ=255)

                                                   gtTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATcgtcagccgtcag                                     >  2:1763747/1‑93 (MQ=255)

                                                      taGTTGCCAGGCATTGCCGCTGACCAGCAATGTGGCGGTTGACTTTGACGCTAAGGGGATGGCATGAGCGAACTGATCGTCAGCGGTCAGCaa                                  <  2:1745803/92‑1 (MQ=255)

                                                      gAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCaa                                  >  2:598937/1‑93 (MQ=255)

                                                      gAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCaa                                  <  1:3480630/93‑1 (MQ=255)

                                                       aGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCAAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAAc                                 <  1:3297386/93‑1 (MQ=255)

                                                        gTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACg                                >  2:2265617/1‑93 (MQ=255)

                                                        gTTGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACg                                >  2:2412698/1‑93 (MQ=255)

                                                         ttGCCAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGa                               <  1:2072743/93‑1 (MQ=255)

                                                            ccAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTa                            <  1:3183598/93‑1 (MQ=255)

                                                            ccAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGACGGAATGAGCGAACTGATCGTCAGCCGTCAGCAACGAgga                            >  1:1246078/1‑91 (MQ=255)

                                                             cAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTAt                           <  2:551356/93‑1 (MQ=255)

                                                             cAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTAt                           <  1:2039981/93‑1 (MQ=255)

                                                             cAGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTAt                           >  2:4244338/1‑93 (MQ=255)

                                                              aGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTAtt                          <  2:230509/93‑1 (MQ=255)

                                                              aGGCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTAtt                          >  2:4126615/1‑93 (MQ=255)

                                                               ggCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTAttg                         <  1:4250197/93‑1 (MQ=255)

                                                                gCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTAttgt                        <  1:1283451/93‑1 (MQ=255)

                                                                gCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTAttgt                        <  1:4407267/93‑1 (MQ=255)

                                                                gCACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTAttgt                        <  2:3377756/93‑1 (MQ=255)

                                                                 cACTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTAttgtt                       <  2:285898/93‑1 (MQ=255)

                                                                   cTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGc                     <  2:565046/93‑1 (MQ=255)

                                                                   cTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGc                     <  1:2809640/93‑1 (MQ=255)

                                                                   cTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGc                     <  2:1876973/93‑1 (MQ=255)

                                                                   cTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGc                     >  1:2190227/1‑93 (MQ=255)

                                                                   cTGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGGATTGTTGc                     >  2:3796710/1‑93 (MQ=255)

                                                                    tGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCt                    <  2:4080119/93‑1 (MQ=255)

                                                                    tGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCt                    <  2:2697574/93‑1 (MQ=255)

                                                                    tGCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGAGGGCATGAGCGAACTGATCGTCAGCCGTCAACAACGAGTCTTGTTGCt                    >  2:1707645/1‑93 (MQ=255)

                                                                     gCCGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTg                   >  1:1277607/1‑93 (MQ=255)

                                                                       cGCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGAc                 >  1:2183579/1‑93 (MQ=255)

                                                                        gCTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGAcc                >  2:3124142/1‑93 (MQ=255)

                                                                         cTGACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGAccc               <  2:3722723/93‑1 (MQ=255)

                                                                           gACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCtt             >  2:2394030/1‑93 (MQ=255)

                                                                           gACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCtt             >  2:2043639/1‑93 (MQ=255)

                                                                           gACCAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCtt             >  1:31282/1‑93 (MQ=255)

                                                                             ccAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTac           >  2:4403787/1‑92 (MQ=255)

                                                                              cAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAAc          <  1:644979/93‑1 (MQ=255)

                                                                              cAGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAAc          <  2:261579/93‑1 (MQ=255)

                                                                               aGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAAcc         >  2:689691/1‑93 (MQ=255)

                                                                               aGCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAAcc         >  1:2277661/1‑93 (MQ=255)

                                                                                gCGATGTGGCGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAAACg        >  1:1414856/1‑93 (MQ=255)

                                                                                      tggcggTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCccgc  >  1:362273/1‑93 (MQ=255)

GATGGAAACCAATTACAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGC  >  NC_000913/1455404‑1455582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`