| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,464,530 | C→T | intergenic (+1445/+1415) | b1400 → / ← yi222 | putative transferase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,464,530 | 0 | C | T | 96.9% | 179.3 / ‑3.0 | 64 | intergenic (+1445/+1415) | b1400/yi222 | putative transferase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (22/40); total (23/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.27e-02 | |||||||||||
TCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAG > NC_000913/1464440‑1464619 | tCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAATgg > 1:1728775/1‑93 (MQ=255) cAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAATGGt < 2:2906870/93‑1 (MQ=255) cAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGGCACGGGATTGTTATCACCGGCGACAGCGCAATGGt > 1:4678783/1‑93 (MQ=14) aCGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAAtggtgg > 1:2347882/1‑93 (MQ=255) aCGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTgg > 2:2549989/1‑93 (MQ=255) cGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAATGGTGGa < 1:1355528/93‑1 (MQ=255) ggTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAATGGTGGAt > 2:2473706/1‑93 (MQ=255) taataaCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAATGGTGGATaa < 1:1356183/93‑1 (MQ=255) aataaCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAATGGTGGATAAc < 2:265523/93‑1 (MQ=255) aataaCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAAc > 1:4562576/1‑93 (MQ=40) ataaCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAAc > 2:765209/1‑82 (MQ=255) ataaCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAATGGTGGATAACa > 1:1532778/1‑93 (MQ=255) ataaCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAATGGTGGATAACa > 2:705268/1‑93 (MQ=255) ataaCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAATGGTGGATAACa < 1:4150238/93‑1 (MQ=255) taaCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAATGGTGGATAACaa < 2:3986418/93‑1 (MQ=255) taaCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAATGGTGGATAACaa > 1:4179038/1‑93 (MQ=255) taaCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAATGGTGGATAACaa > 1:4085451/1‑93 (MQ=255) aaCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAATGGTGGATAACAAg > 1:1620414/1‑93 (MQ=255) aCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAATGGTGGATAACAAgg < 2:2478341/93‑1 (MQ=255) aCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAgg < 2:4562576/93‑1 (MQ=40) cGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAATGGTGGATAACAAggg < 1:2062854/93‑1 (MQ=255) gAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGTCGTCACGGAATTGATATCACCTGCGACAGAGCAATGGTGGATAAGAAGTGCa > 2:1332055/1‑93 (MQ=14) gAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAATGGTGGATAACAAGGGCa > 2:3546068/1‑93 (MQ=255) gAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAATGGTGGATAACAAGGGCa > 1:1058561/1‑93 (MQ=255) gAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAATGGTGGATAACAAGGGCa > 1:4475021/1‑93 (MQ=255) aaaGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGAATTGATATAACCGGTGTCAGCGCAATGGTGGATAACAAGTGCAcc > 2:610773/1‑93 (MQ=11) aaGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAATGGTGGATAACAAGGGCACCa < 1:3611479/93‑1 (MQ=25) aGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAATGGTGGATAACAAGGGCACCAt < 1:3329011/93‑1 (MQ=21) aGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAATGGTGGATAACAAGGGCACCAt < 1:1664352/93‑1 (MQ=21) gTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAATGGTGGATAACAAGGGCACCATg > 1:3324838/1‑93 (MQ=18) ttATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAATGGTGGATAACAAGGGCACCATGa < 2:1212552/93‑1 (MQ=17) gcggTCACGGTATTGATATCACCGGCGACAGCGCAATGGTGGATAACAAGGGCACCATGACCGTCAGCGATCCGGAGTCCATCGGTATCCAgg < 2:3778209/93‑1 (MQ=9) cggTCACGGTATTGATATCACCGGCGACAGCGCAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGt < 1:2026400/93‑1 (MQ=17) tCACGGTATTGATATCACCGGCGACAGCGCAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGa < 2:210297/93‑1 (MQ=18) gcACGGTATTGATATCACCGGCGACAGCGCAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGa < 1:3289068/92‑1 (MQ=18) cACGGTATTGATATCACCGGCGACAGCGCAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGAc < 2:1381730/93‑1 (MQ=21) cACGGTATTGATATCACCGGCGACAGCGCAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGAc < 2:1929331/93‑1 (MQ=18) cACGGTATTGATATCACCGGCGACAGCGCAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGAc < 1:100070/93‑1 (MQ=21) aCGGTATTGATATCACCGGCGACAGCGCAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGt > 2:1999621/1‑88 (MQ=17) aCGGTATTGATATCACCGGCGACAGCGCAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACg > 2:65640/1‑93 (MQ=18) ggTATTGATATCACCGGCGACAGCGCAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGc < 2:4519230/93‑1 (MQ=21) ggTATTGATATCACCGGCGACAGCGCAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGc < 1:3139147/93‑1 (MQ=21) tATTGATATCACCGGCGACAGCGCAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGa < 2:533514/93‑1 (MQ=18) aTTGATATCGCCGGCGACAGCGCAATGGTGGATAACAAGGTCGCCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGAc < 1:2401649/93‑1 (MQ=11) aTTGATATCACCGGCGACAGCGCAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGAc < 2:2857830/93‑1 (MQ=21) aTTGATATCACCGGCGACAGCGCAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGAc < 2:422987/93‑1 (MQ=21) aTTGATATCACCGGCGACAGCGCAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGAc > 2:898572/1‑93 (MQ=18) ttGATATCACCGGCGACAGCGCAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGAcc > 2:327099/1‑93 (MQ=18) tGATATCACCGGCGACAGCGCAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCa < 2:3047366/93‑1 (MQ=21) tGATATCACCGGCGACAGCGCAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCa > 2:3372575/1‑93 (MQ=18) tGATATCACCGGCGACAGCGCAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCa < 1:441480/93‑1 (MQ=21) atatCACCGGCGACAGCGCACTGGTTGATAACAAGGGCACCATGACCGTCACCGACCCGGAGAACATCGGGATCCTTGTTTACGGCGACCAgg > 1:19495/1‑93 (MQ=11) atatCACCGGCGACAGCGCAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAgg > 1:1515782/1‑93 (MQ=18) tatCACCGGCGACAGCGCAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGc < 2:944519/93‑1 (MQ=21) atCACCGGCGACAGCGCAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCg < 1:1633646/93‑1 (MQ=25) atCACCGGCGACAGCGCAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCg < 2:2351315/93‑1 (MQ=25) cACCGGCGACAGCGCAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGgt < 2:2457147/93‑1 (MQ=37) cACCGGCGACAGCGCAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGgt < 1:4535484/93‑1 (MQ=255) cACCGGCGACAGCGCAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGgt > 2:1397734/1‑93 (MQ=37) ccGGCGACAGCGCTATGGTGGATAACAAGGGCACCATGACCGTCACCGATCTGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGgttg < 1:1468/93‑1 (MQ=255) cGGCGACAGCGCAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGgttgt < 1:568410/93‑1 (MQ=255) cGACAGCGCAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTaa < 2:590168/93‑1 (MQ=255) gACAGCGCAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTaaa > 1:4385846/1‑92 (MQ=255) cAGCGCAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTaacaa > 2:3695668/1‑93 (MQ=255) cAGCGCAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTaacaa < 1:3088574/93‑1 (MQ=255) cAGCGCAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTaacaa < 1:2057642/93‑1 (MQ=255) cAGCGCAATGGTGGATAACAAGGACACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTaacaa < 2:2385402/93‑1 (MQ=255) cgcAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGa < 1:2403454/93‑1 (MQ=255) gcAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGaa > 1:1651252/1‑93 (MQ=255) gcAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCCGTTGTTAACAACGaa > 2:4644290/1‑93 (MQ=255) cAATGGTGGATAACATGGTCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAg < 2:1872218/93‑1 (MQ=255) cAATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAg < 2:2033786/93‑1 (MQ=255) | TCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAG > NC_000913/1464440‑1464619 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |