Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 134,838 A→G W249R (TGG→CGG)  speD ← S‑adenosylmethionine decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913134,8380AG99.5% 625.7 / ‑4.6 200W249R (TGG→CGG) speDS‑adenosylmethionine decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (92/107);  minor base T (1/0);  total (93/107)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.85e-01

Only 100 of 213 total aligned reads displayed.

TAAATAAATCTGGCGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAA  >  NC_000913/134748‑134927
                                                                                          |                                                                                         
taaataaaTCTGGCGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCcgca                                                                                         >  2:1001138/1‑93 (MQ=255)
  aataaatCTGGCGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGcagc                                                                                       >  2:3918429/1‑93 (MQ=255)
     aaatCTGGCGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGcagcgca                                                                                    >  2:1590500/1‑93 (MQ=255)
     aaatCTGGCGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGcagcgca                                                                                    <  1:2271937/93‑1 (MQ=255)
       accTGGCGGAGCCTGGGAGCTCCGCCGGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCgcagcgcagc                                                                                  <  2:1643753/91‑1 (MQ=255)
           ggCGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTa                                                                              >  1:407609/1‑93 (MQ=255)
            gCGGATCCTGGGAGCCCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTaa                                                                             <  2:211433/93‑1 (MQ=255)
            gCGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTaa                                                                             <  2:1382314/93‑1 (MQ=255)
            gCGGAGCCTGGGAGCCCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTaa                                                                             <  2:3744174/93‑1 (MQ=255)
            gCGGAGCATGGGAGCTCGGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTaa                                                                             <  1:328685/93‑1 (MQ=255)
             cGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAAt                                                                            <  2:927058/93‑1 (MQ=255)
             cGGAGCCTGGGAGCCCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAAt                                                                            <  1:1285110/93‑1 (MQ=255)
              ggAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAAtt                                                                           <  1:598523/93‑1 (MQ=255)
              ggAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGCAAtt                                                                           >  1:1606441/1‑93 (MQ=255)
               gAGCCTGGGAGCCCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAAttt                                                                          <  2:35098/93‑1 (MQ=255)
                aGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTc                                                                         <  1:2914519/93‑1 (MQ=255)
                aGCCGGGGAGCCCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTc                                                                         <  2:3406823/93‑1 (MQ=255)
                 gCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTct                                                                        >  1:2318845/1‑92 (MQ=255)
                 gCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTct                                                                        >  2:191858/1‑92 (MQ=255)
                 gCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTcc                                                                        <  2:2245496/93‑1 (MQ=255)
                   cTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTg                                                                      >  1:1549248/1‑93 (MQ=255)
                   cTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTg                                                                      <  2:2173813/93‑1 (MQ=255)
                      ggAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGgcg                                                                   <  2:4555305/93‑1 (MQ=255)
                      ggAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGgcg                                                                   >  2:2509816/1‑93 (MQ=255)
                       gAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGgcgc                                                                  >  1:3866593/1‑93 (MQ=255)
                       gAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGgcgc                                                                  >  2:293441/1‑93 (MQ=255)
                       gAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGgcgc                                                                  <  1:4298075/93‑1 (MQ=255)
                       gAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGgcgc                                                                  <  1:2663784/93‑1 (MQ=255)
                       gAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGCAATTTCCTGgcgc                                                                  >  1:4193651/1‑93 (MQ=255)
                        aGCTCCGCCATAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGcgct                                                                 <  2:2322316/93‑1 (MQ=255)
                        aGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGcgct                                                                 <  1:2467985/93‑1 (MQ=255)
                           cccGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGcgctcgc                                                              <  2:371087/92‑1 (MQ=255)
                                  gagCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGt                                                       >  1:473331/1‑93 (MQ=255)
                                  gagCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGt                                                       >  1:2022307/1‑93 (MQ=255)
                                  gagCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGt                                                       >  2:3195933/1‑93 (MQ=255)
                                  gagCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGt                                                       <  2:3586726/93‑1 (MQ=255)
                                    gCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTa                                                     >  2:331099/1‑93 (MQ=255)
                                    gCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTa                                                     >  2:4493882/1‑93 (MQ=255)
                                       gTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGt                                                  >  2:2630274/1‑93 (MQ=255)
                                       gTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGt                                                  >  1:1971941/1‑93 (MQ=255)
                                        ttAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTc                                                 <  1:4230255/93‑1 (MQ=255)
                                        ttAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTc                                                 >  2:1398071/1‑93 (MQ=255)
                                         tAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCt                                                >  2:3430500/1‑93 (MQ=255)
                                           aaCAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTc                                              >  2:200471/1‑93 (MQ=255)
                                             cAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCg                                            <  2:4041652/93‑1 (MQ=255)
                                              aGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCgg                                           >  2:1043785/1‑93 (MQ=255)
                                               gCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGt                                          <  2:1474119/93‑1 (MQ=255)
                                                   gCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTTGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTgg                                      <  2:4448252/93‑1 (MQ=255)
                                                   gCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTgg                                      <  1:1675006/93‑1 (MQ=255)
                                                    cATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGgt                                     <  2:200057/93‑1 (MQ=255)
                                                     atatTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGgtg                                    <  1:1236806/93‑1 (MQ=255)
                                                     atatTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGgtg                                    <  1:3731608/93‑1 (MQ=255)
                                                      tatTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGgtgt                                   >  2:1341726/1‑93 (MQ=255)
                                                      tatTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGgtgt                                   >  1:2409413/1‑93 (MQ=255)
                                                      tatTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGgtgt                                   <  2:3161187/93‑1 (MQ=255)
                                                      tatTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGgtgt                                   >  2:1942921/1‑93 (MQ=255)
                                                      tatTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGgtgt                                   >  1:4319019/1‑93 (MQ=255)
                                                       atTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGtgtg                                  <  2:89900/93‑1 (MQ=255)
                                                        ttGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTgg                                 >  1:148172/1‑93 (MQ=255)
                                                           cgcCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAAc                              >  2:4292789/1‑93 (MQ=255)
                                                            gcCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACa                             >  2:4151798/1‑93 (MQ=255)
                                                             cccGTAATAAATCTCGCGCATTTCTTTCCTCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACAt                            >  1:3535007/1‑93 (MQ=255)
                                                             cccGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACAt                            <  1:3798462/93‑1 (MQ=255)
                                                              ccGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATg                           >  2:1561053/1‑93 (MQ=255)
                                                              ccGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATg                           >  1:3001501/1‑93 (MQ=255)
                                                               cGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGt                          <  1:4463433/93‑1 (MQ=255)
                                                               cGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGt                          <  1:3644320/93‑1 (MQ=255)
                                                               cGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGGTAAGTTTTCCGGTTTGGTGTGGAACATGt                          >  2:630213/1‑93 (MQ=255)
                                                                gTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTa                         >  1:3388854/1‑93 (MQ=255)
                                                                 taataaATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAg                        <  2:1789901/93‑1 (MQ=255)
                                                                 taataaATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAg                        >  1:230214/1‑93 (MQ=255)
                                                                 taataaATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAg                        <  1:1192463/93‑1 (MQ=255)
                                                                 taataaATCTCGCGAATTTCTTTTAGCAGCGCAGCGGTACTTTCCTGTCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGttt                        >  2:3112518/1‑91 (MQ=255)
                                                                  aataaATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGt                       >  2:911025/1‑93 (MQ=255)
                                                                  aataaATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGATTGGTGTGGAACATGTAGt                       <  2:4545485/93‑1 (MQ=255)
                                                                   ataaATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTg                      >  2:1741622/1‑93 (MQ=255)
                                                                   ataaATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTg                      >  1:748788/1‑93 (MQ=255)
                                                                    taaATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGc                     >  2:766577/1‑93 (MQ=255)
                                                                     aaaTCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCt                    <  2:2881718/93‑1 (MQ=255)
                                                                      aaTCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCtt                   >  2:2415286/1‑93 (MQ=255)
                                                                        tctcGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCTCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTaa                 <  1:2591872/93‑1 (MQ=255)
                                                                        tctcGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTaa                 <  1:1110922/93‑1 (MQ=255)
                                                                         ctcGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAg                <  2:182716/93‑1 (MQ=255)
                                                                            gcgcATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTc             <  1:2205392/93‑1 (MQ=255)
                                                                             cgcATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCg            >  1:4678624/1‑93 (MQ=255)
                                                                              gcATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGa           <  1:2757982/93‑1 (MQ=255)
                                                                              gcATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGa           >  1:1143416/1‑93 (MQ=255)
                                                                               cATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGaa          <  1:2106777/93‑1 (MQ=255)
                                                                                 tttctttcCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAAct        >  2:2353333/1‑93 (MQ=255)
                                                                                 tttctttcCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAAct        <  2:2064754/93‑1 (MQ=255)
                                                                                 tttctttcCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAAct        >  2:2740524/1‑93 (MQ=255)
                                                                                  ttctttcCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAActc       <  1:2937308/93‑1 (MQ=255)
                                                                                  ttctttcCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAActc       <  2:475179/93‑1 (MQ=255)
                                                                                   tctttcCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAActct      >  2:1158375/1‑93 (MQ=255)
                                                                                     tttcCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCttt    <  1:1892565/93‑1 (MQ=255)
                                                                                     tttcCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCttt    <  2:4330417/93‑1 (MQ=255)
                                                                                     tttcCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCttt    >  1:2874699/1‑93 (MQ=255)
                                                                                     tttcCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCttt    >  1:1015370/1‑93 (MQ=255)
                                                                                       tcCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTaa  <  2:555022/93‑1 (MQ=255)
                                                                                       tcCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTaa  <  2:4393616/93‑1 (MQ=255)
                                                                                          |                                                                                         
TAAATAAATCTGGCGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAA  >  NC_000913/134748‑134927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑