Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,476,839	T→C	V197A (GTC→GCC)	b1409 →	putative phosphatidate cytidiltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,476,839	0	T	C	100.0%	237.9 / NA	76	V197A (GTC→GCC)	b1409	putative phosphatidate cytidiltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/35);  total (41/35)

GTTACTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGTCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCGGACCGTTA  >  NC_000913/1476750‑1476927                                                                                          |                                                                                         gTTACTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCAGAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGcccc                                                                                       >  1:3574960/1‑93 (MQ=255) gTTACTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGcccc                                                                                       >  2:1708641/1‑93 (MQ=255) gTTACTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGcccc                                                                                       >  1:4492742/1‑93 (MQ=255)  ttACTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCt                                                                                      <  2:4465965/93‑1 (MQ=255)  ttACTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCt                                                                                      <  1:3745883/93‑1 (MQ=255)     cTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTaaa                                                                                   <  1:3741964/93‑1 (MQ=255)      tGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAg                                                                                  <  2:564786/93‑1 (MQ=255)      tGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAg                                                                                  <  1:2139936/93‑1 (MQ=255)         tgtTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCa                                                                               <  1:1036962/93‑1 (MQ=255)         tgtTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCa                                                                               <  2:1714189/93‑1 (MQ=255)         tgtTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCa                                                                               <  1:4072262/93‑1 (MQ=255)           ttATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGc                                                                             >  1:555702/1‑93 (MQ=255)            tATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGcc                                                                            <  1:3991906/93‑1 (MQ=255)             aTTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGacc                                                                           >  1:1287268/1‑93 (MQ=255)              tttCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCg                                                                          >  2:2617200/1‑93 (MQ=255)               ttCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCgg                                                                         >  2:3330246/1‑93 (MQ=255)                tCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCggg                                                                        >  2:2114466/1‑93 (MQ=255)                 cTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGa                                                                       <  2:3844052/93‑1 (MQ=255)                       gCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACa                                                                 >  1:122/1‑93 (MQ=255)                       gCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACa                                                                 <  2:3451003/93‑1 (MQ=255)                         aTTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACAtt                                                               >  2:4061476/1‑93 (MQ=255)                         aTTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACAtt                                                               >  2:1245404/1‑93 (MQ=255)                           tAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTgg                                                             <  2:2922363/93‑1 (MQ=255)                           tAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTgg                                                             >  2:181825/1‑93 (MQ=255)                            aaCCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGa                                                            >  2:1353864/1‑93 (MQ=255)                            aaCCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGa                                                            >  1:3227764/1‑93 (MQ=255)                             aCCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGaa                                                           >  1:2866737/1‑93 (MQ=255)                             aCCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGaa                                                           <  1:568794/93‑1 (MQ=255)                              ccGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAg                                                          <  2:3068875/93‑1 (MQ=255)                               cGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAgg                                                         >  1:727440/1‑93 (MQ=255)                               cGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAgg                                                         >  2:2267365/1‑93 (MQ=255)                                 aGTCAAACGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTc                                                       <  1:2593580/93‑1 (MQ=255)                                    cAAACGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGa                                                    <  1:2892631/93‑1 (MQ=255)                                     aaaCGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGAt                                                   <  1:1608524/93‑1 (MQ=255)                                     aaaCGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGAt                                                   <  2:4388194/93‑1 (MQ=255)                                      aaCGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATg                                                  >  1:3939696/1‑93 (MQ=255)                                      aaCGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATg                                                  >  2:1739511/1‑93 (MQ=255)                                      aaCGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATg                                                  >  1:2124265/1‑93 (MQ=255)                                       aCGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATgg                                                 <  2:2134146/93‑1 (MQ=255)                                        cGATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATggg                                                <  2:2476367/93‑1 (MQ=255)                                         gATATTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGggc                                               >  1:1047505/1‑93 (MQ=255)                                           tatTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGggcgg                                             >  2:2673513/1‑93 (MQ=255)                                            atTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGggcggc                                            >  1:1748352/1‑93 (MQ=255)                                            atTGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGggcggc                                            >  1:1926398/1‑93 (MQ=255)                                              tGCACAGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGt                                          <  2:252421/93‑1 (MQ=255)                                                  caGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGtcatc                                      <  1:417028/93‑1 (MQ=255)                                                  caGTATTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGtcatc                                      <  1:904022/93‑1 (MQ=255)                                                      aTTTATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACca                                  <  2:3462211/93‑1 (MQ=255)                                                       tttATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACcat                                 >  1:1477748/1‑93 (MQ=255)                                                        ttATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACcatc                                >  2:4394982/1‑93 (MQ=255)                                                         tATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACcatca                               <  1:2063114/93‑1 (MQ=255)                                                         tATGGGGAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACcatca                               <  1:802859/93‑1 (MQ=255)                                                            ggggAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGa                            >  1:76016/1‑93 (MQ=255)                                                            ggggAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGa                            <  1:2409781/93‑1 (MQ=255)                                                            ggggAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGa                            >  1:41677/1‑93 (MQ=255)                                                               gAAAATTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTg                         >  1:2849898/1‑93 (MQ=255)                                                                 aaaTTCTGCGGCAGAAGAAAAGTGGCCCGTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCg                       <  1:1018407/93‑1 (MQ=255)                                                                 aaaTTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCg                       >  2:729563/1‑93 (MQ=255)                                                                  aaTTCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGCTTGCGt                      >  2:2423234/1‑93 (MQ=255)                                                                  aaTTCTGCGGCAGAAGAAAAGCGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGt                      >  2:3163391/1‑93 (MQ=255)                                                                    ttcTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCa                    >  1:4508440/3‑93 (MQ=255)                                                                    ttcTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCa                    >  1:2792863/3‑93 (MQ=255)                                                                        gCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGa                <  2:2598810/93‑1 (MQ=255)                                                                          ggCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGAtt              <  1:1200706/93‑1 (MQ=255)                                                                           gCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTa             >  1:3498454/1‑93 (MQ=255)                                                                            cAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTAt            <  2:2932837/93‑1 (MQ=255)                                                                            cAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTAt            >  1:3031893/1‑93 (MQ=255)                                                                             agaagaAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATc           <  1:1412124/93‑1 (MQ=255)                                                                              gaagaaAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCg          <  1:3819390/93‑1 (MQ=255)                                                                              gaagaaAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCg          >  1:514690/1‑93 (MQ=255)                                                                              gaagaaAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCg          >  2:132424/1‑93 (MQ=255)                                                                                 gaaAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCGGAc       >  2:1653899/1‑93 (MQ=255)                                                                                  aaaaGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCGGAcc      >  1:1887814/1‑93 (MQ=255)                                                                                  aaaaGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCGGAcc      >  2:2604191/1‑93 (MQ=255)                                                                                   aaaGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCGGACCg     >  1:681962/1‑93 (MQ=255)                                                                                    aaGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCGGACCGt    <  1:2028263/93‑1 (MQ=255)                                                                                    aaGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCACGATTGCGTCACTGATTATCGGACCGt    >  2:3792679/1‑93 (MQ=255)                                                                                     aGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCGGACCGtt   <  1:3503048/93‑1 (MQ=255)                                                                                      gTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCGGACCGTTa  >  1:2431214/1‑93 (MQ=255)                                                                                          |                                                                                         GTTACTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGTCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCGGACCGTTA  >  NC_000913/1476750‑1476927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑