BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,479,250	T→C	R106R (CGT→CGC)	ynbD →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,479,250	0	T	C	99.0%	300.2 / ‑4.4	98	R106R (CGT→CGC)	ynbD	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (38/59); total (38/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.69e-01

Only 100 of 102 total aligned reads displayed.

ACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCTTCGCTGCATATTAT  >  NC_000913/1479160‑1479339

aCAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGccc                                                                                         <  1:2627303/93‑1 (MQ=255)

 cAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCt                                                                                        <  1:2936214/93‑1 (MQ=255)

  aGCGCCGACTTGTCCACCGGCTTATTCTCGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTg                                                                                       >  2:733053/1‑93 (MQ=255)

     gcCGGCTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTGTATCCGCTGAAGGTTAGTTTTATCCGCCCTGAag                                                                                    >  1:4309905/1‑93 (MQ=255)

      ccGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAagt                                                                                   <  1:1788674/93‑1 (MQ=255)

       cGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAagtg                                                                                  >  1:4254367/1‑93 (MQ=255)

        gACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAagtga                                                                                 <  2:3554923/93‑1 (MQ=255)

         aCTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAagtgag                                                                                >  2:2993215/1‑93 (MQ=255)

         aCTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAagtgag                                                                                <  2:2188827/93‑1 (MQ=255)

         aCTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAagtgag                                                                                >  1:3422904/1‑93 (MQ=255)

          cTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAagtgagt                                                                               <  2:568357/93‑1 (MQ=255)

          cTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAagtgagg                                                                               >  1:3615457/1‑92 (MQ=255)

           ttGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAagtgagtg                                                                              <  2:4152544/93‑1 (MQ=255)

           ttGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAagtgagtg                                                                              <  2:1052750/93‑1 (MQ=255)

           ttGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAagtgagtg                                                                              >  1:3796823/1‑93 (MQ=255)

             gTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTggg                                                                            <  2:4264311/93‑1 (MQ=255)

             gTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTggg                                                                            <  1:3152605/93‑1 (MQ=255)

              tCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTgggg                                                                           <  1:3253718/93‑1 (MQ=255)

               ccACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTggggg                                                                          >  2:2092600/1‑92 (MQ=255)

               ccACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGt                                                                          <  1:1724065/93‑1 (MQ=255)

               ccACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGt                                                                          <  2:4606202/93‑1 (MQ=255)

                 aCCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGACGTGAGTGGGGTGa                                                                        >  1:1779263/1‑93 (MQ=255)

                  ccGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCTGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGAc                                                                       <  1:2510321/93‑1 (MQ=255)

                    ggCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACgg                                                                     <  1:2526998/93‑1 (MQ=255)

                    ggCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACgg                                                                     >  2:46526/1‑93 (MQ=255)

                    ggCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACgg                                                                     <  2:2271557/93‑1 (MQ=255)

                      cTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACgggt                                                                   >  1:460068/1‑92 (MQ=255)

                      cTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGa                                                                   <  2:1213512/93‑1 (MQ=255)

                      cTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGa                                                                   <  1:1417968/93‑1 (MQ=255)

                       ttATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGTAt                                                                  <  2:1810935/93‑1 (MQ=255)

                       ttATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGAt                                                                  <  1:683965/93‑1 (MQ=255)

                        tATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATg                                                                 <  1:2809972/93‑1 (MQ=255)

                         aTTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATgg                                                                >  1:2759292/1‑93 (MQ=255)

                         aTTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATgg                                                                <  2:4269104/93‑1 (MQ=255)

                          ttCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGc                                                               <  2:113091/93‑1 (MQ=255)

                                 aaCGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTc                                                        >  2:1884357/1‑93 (MQ=255)

                                  aCGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCg                                                       >  2:3550687/1‑93 (MQ=255)

                                  aCGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCg                                                       >  1:2867373/1‑93 (MQ=255)

                                   cGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGc                                                      >  2:4299944/1‑93 (MQ=255)

                                     gTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCaa                                                    >  2:2341106/1‑93 (MQ=255)

                                      tAATGGCCTGTTGCTGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAAc                                                   <  2:1278826/93‑1 (MQ=255)

                                         tGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTg                                                >  1:4189758/1‑93 (MQ=255)

                                         tGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTg                                                <  1:3233315/93‑1 (MQ=255)

                                          ggCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGa                                               <  1:4318704/93‑1 (MQ=255)

                                            ccTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAAc                                             <  2:4300645/93‑1 (MQ=255)

                                             ctgctgCGTTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACt                                            <  1:4089735/93‑1 (MQ=255)

                                             ctgctgCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACt                                            <  1:4123733/93‑1 (MQ=255)

                                               gctgcGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGt                                          <  1:193592/93‑1 (MQ=255)

                                               gctgcGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGt                                          <  2:3563299/93‑1 (MQ=255)

                                                ctgcGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGtt                                         >  1:1040870/1‑93 (MQ=255)

                                                ctgcGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGtt                                         <  2:4351016/93‑1 (MQ=255)

                                                 tgcGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGttt                                        >  1:3100691/1‑93 (MQ=255)

                                                 tgcGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGttt                                        <  2:1477587/93‑1 (MQ=255)

                                                 tgcGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGttt                                        <  1:3577995/93‑1 (MQ=255)

                                                 tgcGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGttt                                        >  1:416815/1‑93 (MQ=255)

                                                 tgcGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACCGGATGGCTATTTTCGCAACTTGAACTGttt                                        >  2:1307877/1‑93 (MQ=255)

                                                  gcGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTg                                       >  2:1063816/1‑93 (MQ=255)

                                                   cGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGa                                      <  2:2345005/93‑1 (MQ=255)

                                                    ggTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGAt                                     <  2:4587854/93‑1 (MQ=255)

                                                    ggTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGAt                                     <  2:473419/93‑1 (MQ=255)

                                                    ggTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGGGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGAt                                     >  1:1873682/1‑93 (MQ=255)

                                                     gTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATc                                    >  2:4472548/1‑93 (MQ=255)

                                                       tttttGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTg                                  >  1:846988/1‑93 (MQ=255)

                                                         tttGCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGcc                                <  1:116859/93‑1 (MQ=255)

                                                            gCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTta                             <  1:1437972/93‑1 (MQ=255)

                                                            gCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTta                             >  1:2947684/1‑93 (MQ=255)

                                                            gCTCTATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTta                             <  2:2799870/93‑1 (MQ=255)

                                                             ctctATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTtat                            <  1:4678460/93‑1 (MQ=255)

                                                              tctATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTtata                           <  2:1080740/93‑1 (MQ=255)

                                                              tctATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTtata                           <  2:330923/93‑1 (MQ=255)

                                                               ctATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATaa                          <  2:3079311/93‑1 (MQ=255)

                                                                tATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAAc                         >  1:2359055/1‑93 (MQ=255)

                                                                tATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAAc                         >  1:2743471/1‑93 (MQ=255)

                                                                tATCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGGGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAAc                         >  2:3820086/1‑93 (MQ=255)

                                                                 agccGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAAcc                        <  1:3396430/91‑1 (MQ=255)

                                                                  tCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACTGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCa                       <  1:3943566/93‑1 (MQ=255)

                                                                  tCCGCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCa                       >  1:442565/1‑93 (MQ=255)

                                                                     gCTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGtc                    >  2:437438/1‑93 (MQ=255)

                                                                      cTGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGGAACTTGAACTGTTTGATCTGCCTTATAACCAGtct                   <  1:1672045/93‑1 (MQ=255)

                                                                       tGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGtctc                  >  1:3759223/1‑93 (MQ=255)

                                                                       tGAAGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGtctc                  <  2:2155455/93‑1 (MQ=255)

                                                                         aaGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCt                >  2:2801486/1‑93 (MQ=255)

                                                                         aaGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCt                <  1:2243638/93‑1 (MQ=255)

                                                                         aaGTTTAGTTTTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCt                <  2:4165650/93‑1 (MQ=255)

                                                                          agtttagtttTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCtt               <  1:2922608/93‑1 (MQ=255)

                                                                           gtttagtttTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCTTc              >  1:3418985/1‑93 (MQ=255)

                                                                              tagtttTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCTTCGCt           >  1:2550621/1‑93 (MQ=255)

                                                                                gtttTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCTTCGCTGc         >  2:22478/1‑93 (MQ=255)

                                                                                gtttTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCTTCGCTGc         <  2:2154247/93‑1 (MQ=255)

                                                                                gtttTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCTTCGCTGc         <  2:1851485/93‑1 (MQ=255)

                                                                                gtttTATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCTTCGCTGc         >  1:4120630/1‑93 (MQ=255)

                                                                                  tttATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCTTCGCTGCat       <  1:902014/93‑1 (MQ=255)

                                                                                   ttATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCTTCGCTGCata      <  2:3422904/93‑1 (MQ=255)

                                                                                   ttATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCTTCGCTGCata      <  2:2182902/93‑1 (MQ=255)

                                                                                   ttATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCTTCGCTGCata      <  2:1173659/93‑1 (MQ=255)

                                                                                   ttATCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCTTCGCTGCata      <  1:227487/93‑1 (MQ=255)

                                                                                     aTCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCTTCGCTGCAtatt    <  2:1607754/93‑1 (MQ=255)

                                                                                      tCCGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCTTCGCTGCAtatta   <  2:1659658/93‑1 (MQ=255)

                                                                                       ccGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCTTCGCTGCAtattat  >  1:4544889/1‑93 (MQ=255)

                                                                                       ccGCCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCTTCGCTGCAtattat  >  2:830771/1‑93 (MQ=255)

ACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCTTCGCTGCATATTAT  >  NC_000913/1479160‑1479339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`