Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,498,686	C→T	A30A (GCC→GCT)	tehA →	TehA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,498,686	0	C	T	100.0%	253.6 / NA	71	A30A (GCC→GCT)	tehA	TehA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (49/22);  total (49/22)

GAGCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCCAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACTAGCGCATTTA  >  NC_000913/1498602‑1498774                                                                                     |                                                                                         gagCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGt                                                                                  >  2:192047/1‑93 (MQ=255)    cGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTg                                                                               <  1:3676270/93‑1 (MQ=255)       tAATGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCa                                                                            <  1:1869881/93‑1 (MQ=255)       tAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCa                                                                            >  2:2354786/1‑93 (MQ=255)        aaaGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAg                                                                           >  1:1591140/1‑93 (MQ=255)             gCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAg                                                                      <  1:3274168/93‑1 (MQ=255)             gCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTAAg                                                                      >  1:162367/1‑93 (MQ=255)              cTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTTGCGCTATGCTAGCCAGGGTTGGTAGGTCAgg                                                                     >  1:1886908/1‑92 (MQ=255)              cTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGc                                                                     >  2:202875/1‑93 (MQ=255)               tCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGcc                                                                    >  2:1615484/1‑93 (MQ=255)               tCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGcc                                                                    <  1:3080835/93‑1 (MQ=255)                 aaTTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCAc                                                                  >  2:3096420/1‑93 (MQ=255)                 aaTTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCAc                                                                  >  2:3096419/1‑93 (MQ=255)                   tttGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTg                                                                >  1:3484157/1‑93 (MQ=255)                    ttGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTgg                                                               >  2:3128895/1‑93 (MQ=255)                        cGGCAGGCTACTTTGGTATTGTATTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAg                                                           <  1:3784213/93‑1 (MQ=255)                         ggCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAgg                                                          >  1:2154791/1‑93 (MQ=255)                            aGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGa                                                       >  1:50509/1‑93 (MQ=255)                              gCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATg                                                     >  1:542173/1‑93 (MQ=255)                               cTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGATATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATgg                                                    <  2:2472183/93‑1 (MQ=255)                                tACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATggg                                                   >  1:16302/1‑93 (MQ=255)                                 aCTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGc                                                  >  2:3060642/1‑93 (MQ=255)                                  cTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCt                                                 >  2:3814173/1‑93 (MQ=255)                                   tttGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTg                                                >  1:263921/1‑93 (MQ=255)                                      ggTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTg                                             >  1:3992757/1‑93 (MQ=255)                                       gTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGa                                            >  1:4468132/1‑93 (MQ=255)                                        tATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGAt                                           >  1:2606790/1‑93 (MQ=255)                                        tATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCACCCACTGGGTAGGGGATGGGCCGGTGAt                                           >  1:4013104/1‑93 (MQ=255)                                         aTTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGAtt                                          >  2:4243258/1‑93 (MQ=255)                                          ttGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTc                                         <  2:29868/93‑1 (MQ=255)                                          ttGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTc                                         >  1:2493089/1‑93 (MQ=255)                                          ttGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTc                                         <  2:530775/93‑1 (MQ=255)                                          ttGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTc                                         >  2:3810340/1‑93 (MQ=255)                                          ttGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGGGATTc                                         >  2:130310/1‑93 (MQ=255)                                           tgtgTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCt                                        <  1:1601870/93‑1 (MQ=255)                                            gtgtTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTg                                       >  1:4306759/1‑93 (MQ=255)                                            gtgtTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTg                                       >  2:2799565/1‑93 (MQ=255)                                             tgtTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTgg                                      >  1:1506991/1‑93 (MQ=255)                                               ttGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCg                                    >  2:4647341/1‑93 (MQ=255)                                               ttGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGGGATTCTGGca                                    >  1:1384338/1‑92 (MQ=255)                                                tGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCga                                   <  1:4283240/93‑1 (MQ=255)                                                 ggggACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCgat                                  >  1:1077920/1‑93 (MQ=255)                                                  gggACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCgatg                                 >  2:1285237/1‑93 (MQ=255)                                                    gACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCgatgat                               <  1:2988527/93‑1 (MQ=255)                                                    gACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCgatgat                               <  2:1675536/93‑1 (MQ=255)                                                        aTAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGatcatc                           <  2:2197736/93‑1 (MQ=255)                                                        aTAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGatcatc                           >  1:4604485/1‑93 (MQ=255)                                                         tAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCt                          >  1:3917011/1‑93 (MQ=255)                                                         tAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCt                          >  2:1200631/1‑93 (MQ=255)                                                           gggatgggatTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTgg                        <  2:3805090/93‑1 (MQ=255)                                                            ggatgggatTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTggg                       >  2:3893492/1‑93 (MQ=255)                                                             gatgggatTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATACTGGCGATGATCATCTgggg                      >  1:3177633/1‑93 (MQ=255)                                                              atgggatTTGCCTGGCGCTATGCTAGCCAGGTTCGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGa                     >  1:4079254/1‑93 (MQ=255)                                                               tgggatTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGat                    >  2:1568738/1‑93 (MQ=255)                                                               tgggatTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGat                    >  2:1100550/1‑93 (MQ=255)                                                                 ggatTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGatta                  <  1:3693777/93‑1 (MQ=255)                                                                  gatTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGattat                 >  2:2616216/1‑93 (MQ=255)                                                                  gatTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGattat                 <  1:2385902/93‑1 (MQ=255)                                                                    tttGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGattatta               >  2:1430319/1‑92 (MQ=255)                                                                    tttGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGattatta               >  1:1956455/1‑92 (MQ=255)                                                                     ttGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGa              <  2:3052807/93‑1 (MQ=255)                                                                        ccTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACTa           <  2:1279510/93‑1 (MQ=255)                                                                         cTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACTAg          <  1:1360701/93‑1 (MQ=255)                                                                         cTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACTAg          >  2:3145445/1‑93 (MQ=255)                                                                         cTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACTAg          >  2:1482565/1‑93 (MQ=255)                                                                         cTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACTAg          >  1:2176262/1‑93 (MQ=255)                                                                           ggCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCAGTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACTAgcg        <  1:1500474/93‑1 (MQ=255)                                                                            gcgcTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACTAgcgc       >  2:3579059/1‑93 (MQ=255)                                                                              gcTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACTAGCGCAt     <  1:184525/93‑1 (MQ=255)                                                                               cTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACTAGCGCAtt    <  1:4443242/93‑1 (MQ=255)                                                                               cTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACTAGCGCAtt    <  1:4006065/93‑1 (MQ=255)                                                                                tATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACTAGCGCAttt   >  2:1587845/1‑93 (MQ=255)                                                                                 aTGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACTAGCGCATTTa  >  1:2379137/1‑93 (MQ=255)                                                                                 aTGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACTAGCGCATTTa  <  2:615447/93‑1 (MQ=255)                                                                                     |                                                                                         GAGCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCCAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACTAGCGCATTTA  >  NC_000913/1498602‑1498774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑