Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,516,365	T→C	D169G (GAC→GGC)	b1448 ←	putative resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,516,365	0	T	C	100.0%	291.7 / NA	95	D169G (GAC→GGC)	b1448	putative resistance protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (53/42);  total (53/42)

GATGCGCACCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGTCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCAAATTTAGTGCCTACCTGCGGCATTTGCGCG  >  NC_000913/1516275‑1516454                                                                                           |                                                                                          gATGCGCACCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGccc                                                                                         >  1:3182342/1‑93 (MQ=255)  aTGCGCACCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCg                                                                                        >  1:173879/1‑93 (MQ=255)   tGCGCACCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCgg                                                                                       <  2:3879417/93‑1 (MQ=255)    gcgcACCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGt                                                                                      <  2:2439678/93‑1 (MQ=255)    gcgcACCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGt                                                                                      >  2:216843/1‑93 (MQ=255)         ccATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGt                                                                                 <  2:1033182/93‑1 (MQ=255)          cATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTg                                                                                <  2:3460697/93‑1 (MQ=255)            tCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTgcg                                                                              >  2:769794/1‑93 (MQ=255)            tCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTgcg                                                                              >  2:412422/1‑93 (MQ=255)            tCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTgcg                                                                              >  2:1509441/1‑93 (MQ=255)             cAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTgcgc                                                                             <  1:4397751/93‑1 (MQ=255)             cAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTgcgc                                                                             >  1:429176/1‑93 (MQ=255)              aGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCt                                                                            >  2:3706939/1‑93 (MQ=255)              aGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCt                                                                            >  2:3939175/1‑93 (MQ=255)               gCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCtc                                                                           >  1:774703/1‑93 (MQ=255)                cGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCtcg                                                                          >  2:3665892/1‑93 (MQ=255)                 gtgtTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCtcgt                                                                         <  1:4108957/93‑1 (MQ=255)                 gtgtTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCtcgt                                                                         <  1:1341440/93‑1 (MQ=255)                 gtgtTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGGTCAGTGCGCtcgt                                                                         >  2:3894333/1‑93 (MQ=255)                    ttCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGa                                                                      >  2:2538864/1‑93 (MQ=255)                    ttCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGa                                                                      >  1:4048049/1‑93 (MQ=255)                     tCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAg                                                                     <  1:2231071/93‑1 (MQ=255)                      cTGGACAACCAGACCAATTCAGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGt                                                                    <  1:1662041/93‑1 (MQ=255)                       cggACAACCAGACCAATACCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGtt                                                                   <  2:4380325/92‑1 (MQ=255)                        ggACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTg                                                                  <  1:3207179/93‑1 (MQ=255)                          aCAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCa                                                                >  1:403372/1‑93 (MQ=255)                           cAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCaa                                                               >  1:1424215/1‑93 (MQ=255)                            aaCCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAAc                                                              <  1:1332540/93‑1 (MQ=255)                            aaCCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAAc                                                              <  2:2021161/93‑1 (MQ=255)                            aaCCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAAc                                                              <  2:4223637/93‑1 (MQ=255)                             aCCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACt                                                             <  2:3501074/93‑1 (MQ=255)                               cAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGc                                                           <  2:2130208/93‑1 (MQ=255)                                aGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCa                                                          <  1:1502802/93‑1 (MQ=255)                                aGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCa                                                          >  2:4532117/1‑93 (MQ=255)                                aGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCa                                                          <  1:1502803/93‑1 (MQ=255)                                 gACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAGTCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCAt                                                         <  2:4624538/93‑1 (MQ=255)                                 gACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCAt                                                         >  2:391167/1‑93 (MQ=255)                                  aCCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATa                                                        >  2:722858/1‑93 (MQ=255)                                   ccAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATaa                                                       <  2:439748/93‑1 (MQ=255)                                    cAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATaaa                                                      <  2:1814719/93‑1 (MQ=255)                                    cAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATaaa                                                      <  1:714287/93‑1 (MQ=255)                                    cAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATaaa                                                      >  2:3015660/1‑93 (MQ=255)                                     aaTTCCGGCGGCAATCAACAAAGCAAGGGTGAGCGGCTGATACATCCAATCGCGCCACGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAAt                                                     <  2:3897593/93‑1 (MQ=255)                                     aaTTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAAt                                                     >  1:2698920/1‑93 (MQ=255)                                     aaTTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAAt                                                     <  1:234475/93‑1 (MQ=255)                                      aTTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATg                                                    >  1:4159727/1‑93 (MQ=255)                                      aTTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATg                                                    >  1:3253092/1‑93 (MQ=255)                                       ttCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCACCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATgc                                                   >  1:1476965/1‑92 (MQ=255)                                          cggcggCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAg                                                <  1:1286151/93‑1 (MQ=255)                                           ggcggcAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGa                                               >  2:2730863/1‑93 (MQ=255)                                           ggcggcAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGa                                               >  1:1223399/1‑93 (MQ=255)                                           ggcggcAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGa                                               >  1:2281320/1‑93 (MQ=255)                                            gcggcAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGAt                                              >  1:779102/1‑93 (MQ=255)                                              ggcAATCAAAAAAGCAAGCGTGAGCGACTGATTCATCCAAGCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATcc                                            <  2:4036709/93‑1 (MQ=255)                                                  aTCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGcc                                        >  2:1006331/1‑93 (MQ=255)                                                     aaaaaaGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAAc                                     >  1:111222/1‑93 (MQ=255)                                                       aaaaGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACgc                                   <  2:4502612/93‑2 (MQ=255)                                                       aaaaGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGa                                   >  2:224421/1‑93 (MQ=255)                                                        aaaGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGAc                                  >  1:172369/1‑93 (MQ=255)                                                         aaGCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGAcc                                 >  1:180985/1‑93 (MQ=255)                                                           gCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCaa                               >  2:1947569/1‑93 (MQ=255)                                                           gCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCaa                               <  2:1029389/93‑1 (MQ=255)                                                           gCAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCaa                               >  2:3247088/1‑93 (MQ=255)                                                            cAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCaaa                              <  2:2686437/93‑1 (MQ=255)                                                            cAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCaaa                              <  1:693054/93‑1 (MQ=255)                                                            cAAGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCaaa                              <  2:3197570/93‑1 (MQ=255)                                                             aaGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCAAAt                             >  2:1719126/1‑93 (MQ=255)                                                             aaGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCAAAt                             <  2:121558/93‑1 (MQ=255)                                                              aGGGTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCAAAtt                            <  2:2632924/93‑1 (MQ=255)                                                               gggTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCAAAttt                           >  2:612567/1‑93 (MQ=255)                                                               gggTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCAAAttt                           >  2:4088602/1‑93 (MQ=255)                                                                ggTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCAAATTTa                          <  1:385119/93‑1 (MQ=255)                                                                ggTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCAAATTTa                          <  1:2701217/93‑1 (MQ=255)                                                                 gTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACTAAATTTAg                         <  2:4572114/93‑1 (MQ=255)                                                                 gTGAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCAAATTTAg                         <  1:412422/93‑1 (MQ=255)                                                                   gAGCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCAAATTTAGTg                       <  2:3499683/93‑1 (MQ=255)                                                                     gCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCAAATTTAGTGcc                     <  2:3655/93‑1 (MQ=255)                                                                     gCGACTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCAAATTTAGTGcc                     >  2:2364075/1‑93 (MQ=255)                                                                        aCTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCAAATTTAGTGCCTAc                  >  1:1530166/1‑93 (MQ=255)                                                                        aCTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCAAATTTAGTGCCTAc                  >  2:3412266/1‑93 (MQ=255)                                                                        aCTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCAAATTTAGTGCCTAc                  >  1:3464614/1‑93 (MQ=255)                                                                        aCTGATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCAAATTTAGTGCCTAc                  >  2:1550770/1‑93 (MQ=255)                                                                           gATTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCAAATTTAGTGCCTACCTg               >  2:92563/1‑93 (MQ=255)                                                                            aTTCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCAAATTTAGTGCCTACCTGc              >  1:4407773/1‑93 (MQ=255)                                                                              tCATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCAAATTTAGTGCCTACCTGCgg            <  1:3445417/93‑1 (MQ=255)                                                                               cATCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCAAATTTAGTGCCTACCTGCGGc           >  1:300042/1‑93 (MQ=255)                                                                                aTCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCAAATTTAGTGCCTACCTGCGGCa          >  1:2637301/1‑93 (MQ=255)                                                                                aTCCAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCAAATTTAGTGCCTACCTGCGGCa          >  2:2705484/1‑93 (MQ=255)                                                                                  ccAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCAAATTTAGTGCCTACCTGCGGCAtt        >  1:2844806/1‑93 (MQ=255)                                                                                  ccAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCAAATTTAGTGCCTACCTGCGGCAtt        >  2:4455200/1‑93 (MQ=255)                                                                                   cAATCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGAAACTGCATAAATGTCAGATCCAGCCACCGACCAAATTTAGTGCATAACTGCGGCAttt       >  1:442423/1‑93 (MQ=255)                                                                                    aaTCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCAAATTTAGTGCCTACCTGCGGCATTTg      <  1:2413533/93‑1 (MQ=255)                                                                                    aaTCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCAAATTTAGTGCCTACCTGCGGCATTTg      >  2:90646/1‑93 (MQ=255)                                                                                     aTCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCAAATTTAGTGCCTACCTGCGGCATTTgc     >  2:1626722/1‑93 (MQ=255)                                                                                     aTCGCGCCCAGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCAAATTTAGTGCCTACCTGCGGCATTTgc     >  1:3978110/1‑93 (MQ=255)                                                                                      tCGCGCCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCAAATTTAGTGCCTACCTGCGGCATTTgcg    <  1:4625753/93‑1 (MQ=255)                                                                                        gcgcCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCAAATTTAGTGCCTACCTGCGGCATTTGcgcg  <  2:3392661/93‑1 (MQ=255)                                                                                           |                                                                                          GATGCGCACCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGTCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCCAACGACCAAATTTAGTGCCTACCTGCGGCATTTGCGCG  >  NC_000913/1516275‑1516454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑