| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,568,848 | C→T | A408T (GCC→ACC) | gadB ← | glutamate decarboxylase isozyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,568,848 | 0 | C | T | 98.4% | 145.9 / ‑4.9 | 64 | A408T (GCC→ACC) | gadB | glutamate decarboxylase isozyme |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (29/34); total (30/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.40e-01 | |||||||||||
ATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAGCAAA > NC_000913/1568799‑1568937 | aTAATGCGCTTCACCACGATGTCGTTGCCTGCCCCGCCGAGAGTGAAGGTCGAAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCa < 2:2879139/93‑1 (MQ=11)aTAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCa < 2:2455123/93‑1 (MQ=14) tAATGCGCATCACCACGATGTCGTTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCAt < 1:4468226/93‑1 (MQ=12) tAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCAt > 1:620215/1‑93 (MQ=17) tAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCAt < 1:1634773/93‑1 (MQ=17) aaTGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATa < 1:4371449/93‑1 (MQ=18) aaTGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATa < 2:808296/93‑1 (MQ=18) aaTGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATa < 2:387711/93‑1 (MQ=18) aaTGCGCACCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATa < 2:4226923/93‑1 (MQ=16) tGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACa < 2:3615719/93‑1 (MQ=18) cgcATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAgg < 1:5391/93‑1 (MQ=21) cgcATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAgg > 1:3605066/1‑93 (MQ=18) cgcATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAgg > 2:2924867/1‑93 (MQ=18) gcATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAggg > 1:763847/1‑93 (MQ=18) ccATCACCCCGATGAGGATGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAggg < 2:2426606/92‑1 (MQ=11) ccATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAggg < 1:2874488/92‑1 (MQ=21) cATCACCCCGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGgt < 1:1729262/93‑1 (MQ=255) cATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGgt < 2:2278939/93‑1 (MQ=18) cATCACCACGAGGTCGGTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGgt < 1:111683/93‑1 (MQ=255) tCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGgtgt > 2:1665892/1‑93 (MQ=18) accacGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTAt > 2:2378345/1‑93 (MQ=18) ccacGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATc < 1:873167/93‑1 (MQ=21) cacGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATcc > 1:245340/1‑93 (MQ=18) cacGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGGGGATcc > 2:1361814/1‑93 (MQ=255) aggATGTTGGTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATccc < 1:1081151/91‑1 (MQ=255) ggATGTCGGTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCg < 2:3319230/92‑1 (MQ=18) gATGTCGGTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCgg > 1:1098371/1‑93 (MQ=18) gATGTCGGTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCgg < 1:3336167/93‑1 (MQ=21) aTGTCGGTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGa < 1:510548/93‑1 (MQ=21) aTGTCGGTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGa > 2:638024/1‑93 (MQ=18) aTGTCGGTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGa < 2:1741698/93‑1 (MQ=21) gTCGGTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATc < 2:2655990/93‑1 (MQ=21) tCGGTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCt < 1:3114946/93‑1 (MQ=21) cGGTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCtt < 1:3111302/93‑1 (MQ=18) ggTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTc > 1:58504/1‑93 (MQ=18) ggTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTc < 1:616007/93‑1 (MQ=21) ggTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGGGTATCCCGGATCTTc > 1:2132061/1‑93 (MQ=16) gTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCa > 2:434342/1‑93 (MQ=18) gTGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCa > 2:4451204/1‑93 (MQ=18) tGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCAc < 2:2063863/93‑1 (MQ=21) tGGCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCAc > 2:2420262/1‑93 (MQ=18) ggCTTCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGAGTATCACGGATCTTCAcc > 1:3421867/1‑93 (MQ=255) gCTTCACCGCCGAGAGTGGAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGACATACAGGGTGTATCTCGGATCTTCACCa > 2:1436529/1‑93 (MQ=11) ttCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATc > 1:3901957/1‑93 (MQ=18) ttCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATc > 2:444266/1‑93 (MQ=18) tCACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCt < 1:2609153/93‑1 (MQ=18) ccccgccgAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCtt < 2:1220901/91‑1 (MQ=16) cACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCtt < 1:4519001/93‑1 (MQ=21) cACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCtt > 2:4631299/1‑93 (MQ=18) cACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCtt < 1:2232226/93‑1 (MQ=18) cACCGCCGAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCtt < 1:2275842/93‑1 (MQ=21) ccgccgAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTc > 1:4269317/1‑93 (MQ=18) ccgccgAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTc < 2:1916528/93‑1 (MQ=21) cgccgAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCa < 2:3372249/93‑1 (MQ=21) ccgAGAGTGAAGGTCGGAACCTGCCCGCCGCGGAGACGCAGACGTTCAGAGAGGGCATACAGGGTGTATCCCGGGTCTTCCCCATCTTTCAGt > 2:2038448/1‑93 (MQ=11) ccgAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGt > 2:2068852/1‑93 (MQ=18) ccgAGAGTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGt > 2:1406080/1‑93 (MQ=18) cgAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGtt > 1:506018/1‑93 (MQ=33) gagTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGa > 2:3657306/1‑93 (MQ=18) gagTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGa > 2:702518/1‑93 (MQ=18) gTGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAg > 2:2886000/1‑93 (MQ=18) tGAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAGc > 1:3745377/1‑93 (MQ=18) gAAGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAGCa < 1:324456/93‑1 (MQ=21) aaGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCCCCATCTTTCAGTTTGAAGCaa < 2:2097216/93‑1 (MQ=16) aGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAGCaaa > 1:4477155/1‑93 (MQ=18) aGGTCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAGCaaa < 1:3553412/93‑1 (MQ=21) | ATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAGCAAA > NC_000913/1568799‑1568937 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |