BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,596,967	A→G	L409L (TTA→CTA)	ydeV ←	putative kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,596,967	0	A	G	99.1%	328.1 / ‑4.8	105	L409L (TTA→CTA)	ydeV	putative kinase
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (48/56); total (48/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 107 total aligned reads displayed.

TGACCACCGGAATATTGACGGGTAATCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAACGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACA  >  NC_000913/1596877‑1597056

tGACCACCGGAATATTGACGGGTAATCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCg                                                                                         <  1:2715030/93‑1 (MQ=255)

  accaccGGAATATTGACGGGTAATCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGAt                                                                                       <  2:4097548/93‑1 (MQ=255)

   ccaccGGAATATTGACGGGTAATCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATg                                                                                      <  2:3733246/93‑1 (MQ=255)

   ccaccGGAATATTGACGGGTAATCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATg                                                                                      >  2:4292627/1‑93 (MQ=255)

    caccGGAATATTGACGGGTAATCCCGAGACATCAGCGAGAATTTGACTGCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGa                                                                                     >  1:3183850/1‑93 (MQ=255)

    caccGGAATATTGACGGGTAATCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGa                                                                                     >  1:3255446/1‑93 (MQ=255)

    caccGGAATATTGACGGGTAATCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGa                                                                                     >  1:548529/1‑93 (MQ=255)

      ccGGAATATTGACGGGTAATCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAg                                                                                   <  2:389132/93‑1 (MQ=255)

        ggAATATTGACGGGTAATCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGa                                                                                 <  2:1114840/93‑1 (MQ=255)

        ggAATATTGACGGGTAATCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGa                                                                                 >  1:3757702/1‑93 (MQ=255)

        ggAATATTGACGGGTAATCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGa                                                                                 >  1:988764/1‑93 (MQ=255)

         gAATATTGACGGGTAATCCCGAGACATCAGCGAGAATTTGACTCCATCATTTCCCTTTTGAACCTCCGCATGCAAAGACTAGCGATGAAGGAt                                                                                <  1:895207/93‑1 (MQ=255)

         gAATATTGACGGGTAATCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGAt                                                                                >  2:1850424/1‑93 (MQ=255)

         gAATATTGACGGGTAATCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGAt                                                                                <  2:3571824/93‑1 (MQ=255)

         gAATATTGACGGGTAATCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGAt                                                                                <  2:355677/93‑1 (MQ=255)

         gAATATTGACGGGTAATCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGAt                                                                                <  2:885758/93‑1 (MQ=255)

          aaTATTGACGGGTAATCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATg                                                                               <  2:3373707/93‑1 (MQ=255)

            tatTGACGGGTAATCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGaa                                                                             >  1:247451/1‑93 (MQ=255)

            tatTGACGGGTAATCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGaa                                                                             <  2:1652928/93‑1 (MQ=255)

              ttGACGGGTAATCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAata                                                                           <  2:867786/93‑1 (MQ=255)

              ttGACGGGTAATCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAata                                                                           <  2:2851669/93‑1 (MQ=255)

              ttGACGGGTAATCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAata                                                                           <  2:555859/93‑1 (MQ=255)

                gACGGGTAATCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATAtt                                                                         <  1:632434/93‑1 (MQ=255)

                gACGGGTAATCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATAtt                                                                         <  2:4556367/93‑1 (MQ=255)

                   gggTAATCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCga                                                                      >  1:3362523/1‑93 (MQ=255)

                    ggTAATCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCgag                                                                     >  1:514577/1‑93 (MQ=255)

                     gTAATCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCgaga                                                                    <  1:1263221/93‑1 (MQ=255)

                     gTAATCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCgaga                                                                    <  1:66293/93‑1 (MQ=255)

                      tAATCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGaa                                                                   <  2:1236090/93‑1 (MQ=255)

                      tAATCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGaa                                                                   >  1:1914442/1‑93 (MQ=255)

                      tAATCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGaa                                                                   >  2:3198510/1‑93 (MQ=255)

                      tAATCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGaa                                                                   <  1:522283/93‑1 (MQ=255)

                        aTCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAAt                                                                 >  1:1066451/1‑93 (MQ=255)

                        aTCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAAt                                                                 <  1:529412/93‑1 (MQ=255)

                         tCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATc                                                                <  2:3241052/93‑1 (MQ=255)

                         tCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATc                                                                <  2:3069040/93‑1 (MQ=255)

                         tCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATc                                                                <  2:1507856/93‑1 (MQ=255)

                          cccGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCa                                                               <  1:2582041/93‑1 (MQ=255)

                              agaCATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCaa                                                           <  2:3710095/93‑1 (MQ=255)

                              agaCATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCaa                                                           <  1:1854412/93‑1 (MQ=255)

                              agaCAGCAGCGGGAGTTGTGCTCCATAATTTCGCTGTTGATCCTCCGCCTGCAAAGACTAGCGGGGAAGGATGAGTATTCGAGAAATCAGCaa                                                           <  2:564999/93‑1 (MQ=255)

                               gaCATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAAt                                                          >  1:2552026/1‑93 (MQ=255)

                                aCATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAAtt                                                         <  2:3209965/93‑1 (MQ=255)

                                 cATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAAttt                                                        >  1:3364540/1‑93 (MQ=255)

                                  aTCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTtg                                                       >  1:3539349/1‑93 (MQ=255)

                                   tCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTtgc                                                      >  2:3245153/1‑93 (MQ=255)

                                   tCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTtgc                                                      <  2:4319079/93‑1 (MQ=255)

                                   tCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTtgc                                                      >  1:2175251/1‑93 (MQ=255)

                                    cAGCTAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTtgct                                                     <  2:1932683/93‑1 (MQ=255)

                                     aGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTtgctg                                                    >  2:4061267/1‑93 (MQ=255)

                                        gagaATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCaa                                                 <  2:4349447/93‑1 (MQ=255)

                                        gagaATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCaa                                                 >  2:1265440/1‑93 (MQ=255)

                                        gagaATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCaa                                                 <  1:47317/93‑1 (MQ=255)

                                        gagaATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCaa                                                 >  1:4073260/1‑93 (MQ=255)

                                             tttGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTac                                            <  2:4265260/93‑1 (MQ=255)

                                              ttGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAATTTaca                                           <  1:4403074/93‑1 (MQ=255)

                                              ttGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTaca                                           <  1:3503967/93‑1 (MQ=255)

                                                gACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAACTTAAACg                                         >  2:1690667/1‑93 (MQ=255)

                                                  cTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCt                                       >  2:2355563/1‑93 (MQ=255)

                                                  cTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCt                                       >  2:2541709/1‑93 (MQ=255)

                                                   tCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTg                                      <  2:3076310/93‑1 (MQ=255)

                                                     cATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGAt                                    >  1:4681304/1‑93 (MQ=255)

                                                      aTAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATa                                   >  1:3851939/1‑93 (MQ=255)

                                                       tAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATAc                                  >  1:1452733/1‑93 (MQ=255)

                                                        aaTTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACa                                 <  2:4338974/93‑1 (MQ=255)

                                                        aaTTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACa                                 >  1:4092033/1‑93 (MQ=255)

                                                        aaTTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACa                                 >  2:1351215/1‑93 (MQ=255)

                                                         aTTTCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGTATTAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACCCGCTGATACaa                                <  2:440106/93‑1 (MQ=255)

                                                          tttCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAAt                               >  2:4059869/1‑93 (MQ=255)

                                                           ttCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATc                              <  1:1514140/93‑1 (MQ=255)

                                                           ttCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATc                              >  2:2854649/1‑93 (MQ=255)

                                                            tCCCTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATcg                             >  2:4077116/1‑93 (MQ=255)

                                                             cccTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATcgc                            >  2:2361598/1‑93 (MQ=255)

                                                              ccTTTTGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATcgcc                           >  2:4034518/1‑93 (MQ=255)

                                                                  ttGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCAt                       >  2:744572/1‑93 (MQ=255)

                                                                   tGAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCAtt                      <  2:636663/93‑1 (MQ=255)

                                                                   tGAACCTCCGCCTGCAAAGACTAACGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCAtt                      <  1:4409197/93‑1 (MQ=255)

                                                                    gAACCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCAttt                     <  2:1707964/93‑1 (MQ=255)

                                                                      aCCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTttc                   >  2:312461/1‑93 (MQ=255)

                                                                      aCCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTttc                   <  1:3950394/93‑1 (MQ=255)

                                                                      aCCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTttc                   >  2:1611787/1‑93 (MQ=255)

                                                                      aCCTCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTttc                   <  2:409015/93‑1 (MQ=255)

                                                                         tCCGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTttcttc                >  1:1112128/1‑93 (MQ=255)

                                                                           cGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCa              <  2:2118952/93‑1 (MQ=255)

                                                                           cGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCa              <  2:3821931/93‑1 (MQ=255)

                                                                           cGCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCa              <  1:3333513/93‑1 (MQ=255)

                                                                            gCCTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCCTCCAg             <  2:3055578/93‑1 (MQ=255)

                                                                             ccTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAgc            >  1:4569889/1‑93 (MQ=255)

                                                                              cTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAgcg           >  2:3749420/1‑93 (MQ=255)

                                                                              cTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAgcg           >  2:278002/1‑93 (MQ=255)

                                                                              cTGCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAgcg           <  1:824868/93‑1 (MQ=255)

                                                                                gCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCa         >  2:82569/1‑93 (MQ=255)

                                                                                gCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCa         >  1:1060432/1‑93 (MQ=255)

                                                                                gCAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCa         >  2:166837/1‑93 (MQ=255)

                                                                                 cAAAGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCAc        <  1:3791028/93‑1 (MQ=255)

                                                                                  aaaGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACg       >  1:2278607/1‑93 (MQ=255)

                                                                                   aaGACTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACgg      >  2:3133012/1‑93 (MQ=255)

                                                                                      aCTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAAc   <  1:3004942/93‑1 (MQ=255)

                                                                                      aCTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAAc   <  2:267685/93‑1 (MQ=255)

                                                                                       cTAGCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACa  >  1:3543926/1‑93 (MQ=255)

TGACCACCGGAATATTGACGGGTAATCCCGAGACATCAGCGAGAATTTGACTCCATAATTTCCCTTTTGAACCTCCGCCTGCAAAGACTAACGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACA  >  NC_000913/1596877‑1597056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`