Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,599,943	G→A	A144T (GCC→ACC)	b1513 →	putative ATP‑binding component of a transport system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,599,943	0	G	A	100.0%	274.4 / NA	81	A144T (GCC→ACC)	b1513	putative ATP‑binding component of a transport system
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/40);  total (41/40)

AAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCGCCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTACCCC  >  NC_000913/1599853‑1600031                                                                                           |                                                                                         aaaCAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCAcc                                                                                        >  2:3001188/1‑93 (MQ=255) aaaCAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCAcc                                                                                        <  2:4171448/93‑1 (MQ=255)  aaCAGCTCTNCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTNGNCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCg                                                                                       >  1:4133068/1‑93 (MQ=255)   aCAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGa                                                                                      >  1:2392673/1‑93 (MQ=255)    cAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGAt                                                                                     <  2:556549/93‑1 (MQ=255)    cAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGAt                                                                                     <  2:3907365/93‑1 (MQ=255)    cAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGAt                                                                                     <  2:1924866/93‑1 (MQ=255)       ctctCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGc                                                                                  >  1:2462109/1‑93 (MQ=255)         ctcCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCa                                                                                <  1:2556385/93‑1 (MQ=255)          tcCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCaa                                                                               >  1:4574730/1‑93 (MQ=255)          tcCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCaa                                                                               >  2:2098415/1‑93 (MQ=255)           ccATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCaaa                                                                              >  1:2711311/1‑93 (MQ=255)           ccATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCaaa                                                                              <  2:642623/93‑1 (MQ=255)            cATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAAt                                                                             >  1:4412628/1‑93 (MQ=255)             aTGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAAtg                                                                            <  1:3942742/93‑1 (MQ=255)              tGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAAtgg                                                                           <  1:1498310/93‑1 (MQ=255)                cAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAAtggtg                                                                         <  2:3566569/93‑1 (MQ=255)                 aGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGGTCGCCAAAtggtgg                                                                        >  1:1124451/1‑93 (MQ=255)                  gAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGa                                                                       <  1:780376/93‑1 (MQ=255)                    aaaTGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGaaa                                                                     >  2:1976369/1‑93 (MQ=255)                      aTGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAAtc                                                                   >  1:901128/1‑93 (MQ=255)                      aTGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAAtc                                                                   <  2:1081073/93‑1 (MQ=255)                       tGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAAtcc                                                                  <  2:2924027/93‑1 (MQ=255)                       tGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAAtcc                                                                  >  1:3618396/1‑93 (MQ=255)                       tGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAAtcc                                                                  >  1:4284193/1‑93 (MQ=255)                        gaagaaCTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAAtcct                                                                 <  1:444639/93‑1 (MQ=255)                        gaagaaCTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAAtcct                                                                 >  2:1917056/1‑93 (MQ=255)                         aagaaCTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAAtcctc                                                                <  2:2978003/93‑1 (MQ=255)                          agaaCTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAAtcctcc                                                               <  1:4362694/93‑1 (MQ=255)                           gaaCTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGGTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCcg                                                              <  1:1346060/93‑1 (MQ=255)                            aaCTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCcgc                                                             >  2:355871/1‑93 (MQ=255)                             aCTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCcgcg                                                            >  2:898328/1‑93 (MQ=255)                                  cTGGCGGCGGTGGGCTGCCAGTTTGATCTGCATTGTATGGCAGGATAGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTACACGGGGTg                                                       >  2:2765992/1‑93 (MQ=255)                                  cTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTg                                                       <  2:1018845/93‑1 (MQ=255)                                      cggcgCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATgc                                                   <  1:3532958/93‑1 (MQ=255)                                      cggcgCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATgc                                                   <  1:4500300/93‑1 (MQ=255)                                       ggcgCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATgcg                                                  <  1:997119/93‑1 (MQ=255)                                        gcgcTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATgcgc                                                 <  2:1583018/93‑1 (MQ=255)                                        gcgcTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATgcgc                                                 >  1:1309805/1‑93 (MQ=255)                                         cgcTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGcgcg                                                <  2:3176914/93‑1 (MQ=255)                                         cgcTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGcgcg                                                >  1:4064795/1‑93 (MQ=255)                                             gggCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTc                                            <  1:3827162/93‑1 (MQ=255)                                                 tGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCgg                                        <  1:3479968/93‑1 (MQ=255)                                                   ccAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCACGGGCTGATGCGCGACTCGCGGAt                                      >  1:2490885/1‑93 (MQ=255)                                                     aGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTc                                    >  1:2913375/1‑93 (MQ=255)                                                      gTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCt                                   >  1:3783315/1‑93 (MQ=255)                                                      gTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCt                                   >  2:2564178/1‑93 (MQ=255)                                                       tttGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTg                                  <  2:362114/93‑1 (MQ=255)                                                        ttGAGCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGa                                 <  2:2201204/93‑1 (MQ=255)                                                         tGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGAt                                <  1:3210453/93‑1 (MQ=255)                                                         tGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGAt                                >  1:1891610/1‑93 (MQ=255)                                                         tGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGAt                                <  1:3274853/93‑1 (MQ=255)                                                           aTCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGGTTCTGATcc                              >  1:4357858/1‑93 (MQ=255)                                                           aTCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATcc                              <  1:438008/93‑1 (MQ=255)                                                           aTCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATcc                              >  2:1004184/1‑93 (MQ=255)                                                            tCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCt                             >  2:2260638/1‑93 (MQ=255)                                                             cTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTc                            >  2:2549678/1‑93 (MQ=255)                                                               gCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGa                          <  1:4584103/93‑1 (MQ=255)                                                                 aTAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATg                        >  2:2360778/1‑93 (MQ=255)                                                                  tAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGa                       <  2:3114907/93‑1 (MQ=255)                                                                  tAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGa                       >  2:3048385/1‑93 (MQ=255)                                                                   aGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGaa                      >  1:4201674/1‑93 (MQ=255)                                                                    gTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAAc                     >  2:815611/1‑93 (MQ=255)                                                                     tCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAAcc                    <  1:3250583/93‑1 (MQ=255)                                                                     tCTGGCAGGATCGCTGGATGCCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAAcc                    <  1:3368006/93‑1 (MQ=255)                                                                       tGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTa                  <  1:2153143/93‑1 (MQ=255)                                                                        ggCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTAc                 <  2:4222397/93‑1 (MQ=255)                                                                         gCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTAcc                >  2:4108541/1‑93 (MQ=255)                                                                         gCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTAcc                <  1:1232963/93‑1 (MQ=255)                                                                         gCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTAcc                <  2:1178894/93‑1 (MQ=255)                                                                         gCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTAcc                <  1:1507118/93‑1 (MQ=255)                                                                           aGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGc              >  1:799727/1‑93 (MQ=255)                                                                           aGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGc              >  1:4194306/1‑93 (MQ=255)                                                                            ggATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCggc             >  1:3712990/1‑91 (MQ=255)                                                                            ggATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGcc             >  2:3089676/1‑93 (MQ=255)                                                                            ggATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGcc             <  2:1160935/93‑1 (MQ=255)                                                                             gATCGCTGTATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCt            <  2:4428632/93‑1 (MQ=255)                                                                             gATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCt            >  2:3022973/1‑93 (MQ=255)                                                                              aTCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTc           >  2:4417603/1‑93 (MQ=255)                                                                               tCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGCTCCTCGATAAACCTACCGCCTCg          <  2:1300002/93‑1 (MQ=255)                                                                               tCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCg          >  2:1216218/1‑93 (MQ=255)                                                                                   tGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCCACTCGCGGACTCTGATCCTCGATGAAACTACCGCCTCGCTTa      >  1:1733459/1‑93 (MQ=255)                                                                                      aTGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTAccc   >  2:2844848/1‑93 (MQ=255)                                                                                      aTGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTAccc   >  1:540483/1‑93 (MQ=255)                                                                                       tGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTAcccc  <  1:2940403/93‑1 (MQ=255)                                                                                           |                                                                                         AAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCGCCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTACCCC  >  NC_000913/1599853‑1600031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑