Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,605,212	T→C	F64L (TTC→CTC)	b1518 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,605,212	0	T	C	100.0%	232.7 / NA	76	F64L (TTC→CTC)	b1518	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (46/30);  total (46/30)

GGCAATTTGCGCTTCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTT  >  NC_000913/1605122‑1605302                                                                                           |                                                                                           ggCAATTTGCGCTTCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTc                                                                                          >  2:3549579/1‑93 (MQ=255)   cAATTTGCGCTTCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCa                                                                                        >  1:955858/1‑93 (MQ=255)    aaTTTGCGCTTCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCAt                                                                                       >  1:1138946/1‑93 (MQ=255)    aaTTTGCGCTTCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCAt                                                                                       >  2:282787/1‑93 (MQ=255)     aTTTGCGCTTCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATa                                                                                      <  2:2409822/93‑1 (MQ=255)      tttGCGCTTCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATaa                                                                                     >  2:3850187/1‑93 (MQ=255)      tttGCGCTTCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATaa                                                                                     >  1:463876/1‑93 (MQ=255)            cTTCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCAc                                                                               >  1:4627788/1‑93 (MQ=255)             ttCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACg                                                                              >  2:4120681/1‑93 (MQ=255)             ttCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGGGGCGCTACATAAAACCACg                                                                              >  1:1014759/1‑93 (MQ=255)                   gTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCAc                                                                        <  2:3101549/93‑1 (MQ=255)                    tCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACt                                                                       <  2:1126598/93‑1 (MQ=255)                     cTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTa                                                                      >  1:1553459/1‑93 (MQ=255)                      ttACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTAc                                                                     <  1:2743018/93‑1 (MQ=255)                      ttACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTAc                                                                     >  1:4484481/1‑93 (MQ=255)                        aCAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACaa                                                                   <  2:2106231/93‑1 (MQ=255)                            gACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAAcc                                                               <  1:873386/93‑1 (MQ=255)                            gACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAAcc                                                               <  2:1600962/93‑1 (MQ=255)                             aCCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCt                                                              >  1:847198/1‑93 (MQ=255)                               ccGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCtgt                                                            >  1:4241383/1‑93 (MQ=255)                                cGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCtgtg                                                           <  1:326917/93‑1 (MQ=255)                                 ggAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTgtgt                                                          >  1:4136203/1‑93 (MQ=255)                                 ggAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTgtgt                                                          >  1:2144853/1‑93 (MQ=255)                                    aGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTcgc                                                       <  2:2093435/93‑1 (MQ=255)                                    aGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTcgc                                                       <  1:780145/93‑1 (MQ=255)                                        aaTTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGaaa                                                   >  2:1525376/1‑93 (MQ=255)                                        aaTTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGaaa                                                   >  2:4633129/1‑93 (MQ=255)                                        aaTTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGaaa                                                   >  1:188377/1‑93 (MQ=255)                                          ttCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCAT‑AAACCACGCCCCACTACAAAAACTGGGGCGCGGAACTg                                                >  2:3761530/1‑93 (MQ=255)                                           tCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTg                                                <  2:2707800/93‑1 (MQ=255)                                            cgcgCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTgg                                               <  2:1833669/93‑1 (MQ=255)                                                cTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATc                                           <  1:14659/93‑1 (MQ=255)                                                cTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATc                                           >  1:922023/1‑93 (MQ=255)                                                cTTTTATAGCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATc                                           <  1:1258337/93‑1 (MQ=255)                                                 ttttATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCt                                          <  1:1773959/93‑1 (MQ=255)                                                    tataTCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTa                                       >  1:4348129/1‑93 (MQ=255)                                                    tataTCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTa                                       <  2:3383872/93‑1 (MQ=255)                                                    tataTCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCCTTa                                       >  2:4354031/1‑93 (MQ=255)                                                      tatCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAAt                                     <  2:2690605/93‑1 (MQ=255)                                                         cTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGAc                                  <  1:845190/93‑1 (MQ=255)                                                         cTACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGAc                                  >  1:2299504/1‑93 (MQ=255)                                                          tACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGAcc                                 >  1:781957/1‑93 (MQ=255)                                                          tACGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGAcc                                 <  2:587485/93‑1 (MQ=255)                                                            cGAAGCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCgg                               <  2:641671/93‑1 (MQ=255)                                                                gCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGCAACTGGAATCTTTAATGACCGGGCcg                           >  2:1783560/1‑93 (MQ=255)                                                                gCCTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCcg                           >  2:4250227/1‑93 (MQ=255)                                                                 ccTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCcgc                          >  1:759315/1‑93 (MQ=255)                                                                 ccTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCcgc                          >  1:2937688/1‑93 (MQ=255)                                                                 ccTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCcgc                          >  2:2542196/1‑93 (MQ=255)                                                                  cTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGAccgcgccgcg                         >  2:1930363/1‑93 (MQ=255)                                                                  cTATAAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCcgcg                         >  1:1313509/1‑93 (MQ=255)                                                                    ataAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTa                       >  2:3046698/1‑93 (MQ=255)                                                                    ataAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTa                       >  2:539127/1‑93 (MQ=255)                                                                    ataAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTa                       >  2:890618/1‑93 (MQ=255)                                                                    ataAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTa                       <  2:1816357/93‑1 (MQ=255)                                                                     taAAGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTaa                      >  2:1462860/1‑93 (MQ=255)                                                                      aaaGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTaaa                     <  1:3506740/93‑1 (MQ=255)                                                                       aaGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTaaaa                    >  2:4629295/1‑93 (MQ=255)                                                                       aaGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTaaaa                    >  2:3324431/1‑93 (MQ=255)                                                                        aGATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTaaaaa                   <  2:1018521/93‑1 (MQ=255)                                                                         gATGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTaaaaac                  >  1:4522985/1‑92 (MQ=255)                                                                          aTGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTaaaaacg                 >  1:2905036/1‑91 (MQ=255)                                                                          aTGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTaaaaacg                 >  2:3039820/1‑91 (MQ=255)                                                                           tGAAGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTaaaaacgt                <  1:2919166/93‑4 (MQ=255)                                                                             aaGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGT‑AAAAACGTCt             <  2:1354732/93‑1 (MQ=255)                                                                             aaGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGT‑AAAAACGTCt             >  1:2173/1‑93 (MQ=255)                                                                             aaGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGT‑AAAAACGTCt             <  2:2670512/93‑1 (MQ=255)                                                                             aaGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGT‑AAAAACGTCt             >  2:2995820/1‑93 (MQ=255)                                                                              aGACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGT‑AAAAACGTCTg            >  2:2588111/1‑93 (MQ=255)                                                                               gACGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGT‑AAAAACGTCTGt           >  1:990734/1‑93 (MQ=255)                                                                                 cGCAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCG‑AGAAAACGTCTGTTc         >  1:2677421/1‑93 (MQ=255)                                                                                   cAGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGT‑AAAAACGTCTGTTCaa       <  2:3445225/93‑1 (MQ=255)                                                                                    aGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGT‑AAAAACGTCTGTTCAAt      <  2:4023916/93‑1 (MQ=255)                                                                                    aGTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGT‑AAAAACGTCTGTTCAAt      <  2:1892868/93‑1 (MQ=255)                                                                                     gTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGT‑AAAAACGTCTGTTCAATg     >  1:3160439/1‑93 (MQ=255)                                                                                     gTGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGT‑AAAAACGTCTGTTCAATg     >  2:2956349/1‑93 (MQ=255)                                                                                      tGGCGCTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGT‑AAAAACGTCTGTTCAATgg    <  1:4530207/93‑1 (MQ=255)                                                                                        gcgcTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGT‑AAAAACGTCTGTTCAATGGtt  <  2:1548706/93‑1 (MQ=255)                                                                                        gcgcTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGT‑AAAAACGTCTGTTCAATGGtt  <  2:181399/93‑1 (MQ=255)                                                                                           |                                                                                           GGCAATTTGCGCTTCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTT  >  NC_000913/1605122‑1605302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑