Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,612,901	T→C	V25A (GTG→GCG)	yneJ →	putative transcriptional regulator LYSR‑type

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,612,901	0	T	C	100.0%	260.3 / NA	85	V25A (GTG→GCG)	yneJ	putative transcriptional regulator LYSR‑type
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/44);  total (41/44)

ACGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATCTGTTTATTCGCGAGAATCAGCGT  >  NC_000913/1612812‑1612989                                                                                          |                                                                                         aCGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCa                                                                                       >  2:876461/1‑93 (MQ=255)  cGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCAt                                                                                      >  1:550958/1‑93 (MQ=255)  cGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCAt                                                                                      <  2:342224/93‑1 (MQ=255)   gAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATc                                                                                     <  2:180153/93‑1 (MQ=255)     aTCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATcgc                                                                                   >  1:4219959/1‑93 (MQ=255)      tCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATcgcg                                                                                  >  1:4672117/1‑93 (MQ=255)        gagaATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTg                                                                                >  1:2600317/1‑93 (MQ=255)          gaATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGcc                                                                              >  1:298510/1‑93 (MQ=255)          gaATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGcc                                                                              >  2:3704362/1‑93 (MQ=255)           aaTGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCg                                                                             >  2:212040/1‑93 (MQ=255)            aTGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGt                                                                            <  2:1764469/93‑1 (MQ=255)            aTGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGt                                                                            >  2:4398670/1‑93 (MQ=255)            aTGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGt                                                                            >  1:1653162/1‑93 (MQ=255)             tGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTc                                                                           >  1:4607258/1‑93 (MQ=255)             tGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTc                                                                           >  1:1065569/1‑93 (MQ=255)                tATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGaa                                                                        >  1:4444714/1‑93 (MQ=255)                   ggATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAAtct                                                                     >  2:1705454/1‑93 (MQ=255)                    gATCTGACCCAACTGAAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAAtctc                                                                    <  2:2645835/93‑1 (MQ=255)                     aTCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCa                                                                   <  2:671732/93‑1 (MQ=255)                     aTCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCa                                                                   <  1:3511871/93‑1 (MQ=255)                      tCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCAc                                                                  >  1:1644192/1‑93 (MQ=255)                      tCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCAc                                                                  <  1:4433977/93‑1 (MQ=255)                       cTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACt                                                                 >  2:3413836/1‑93 (MQ=255)                        tGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTa                                                                >  1:2144295/1‑93 (MQ=255)                          aCCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTAcc                                                              <  1:1009875/93‑1 (MQ=255)                           cccAGCTGGAGATGTGCAGCGCTGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTAccc                                                             <  2:494405/93‑1 (MQ=255)                             cAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGt                                                           <  2:618904/93‑1 (MQ=255)                              aaCTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTc                                                          <  1:820481/93‑1 (MQ=255)                              aaCTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTc                                                          <  2:2968064/93‑1 (MQ=255)                              aaCTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAACATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTc                                                          >  2:2054029/1‑93 (MQ=255)                               aCTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCt                                                         >  1:2318266/1‑93 (MQ=255)                                cTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTg                                                        <  1:3154096/93‑1 (MQ=255)                                cTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTg                                                        >  2:4427284/1‑93 (MQ=255)                                 tGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTgc                                                       >  1:2807508/1‑93 (MQ=255)                                   gagaTGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTgcgc                                                     <  2:4097567/93‑1 (MQ=255)                                   gagaTGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTgcgc                                                     >  1:3613239/1‑93 (MQ=255)                                    agaTGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGcc                                                    <  2:2643425/93‑1 (MQ=255)                                     gaTGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGccg                                                   >  1:152732/1‑92 (MQ=255)                                     gaTGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCa                                                   <  2:1817247/93‑1 (MQ=255)                                      aTGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAg                                                  >  1:4393/1‑93 (MQ=255)                                       tGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGc                                                 <  1:511147/93‑1 (MQ=255)                                       tGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGc                                                 <  1:3011001/93‑1 (MQ=255)                                        gTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCt                                                <  2:3183673/93‑1 (MQ=255)                                            aaCGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGaa                                            >  1:2081502/1‑93 (MQ=255)                                            aaCGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGaa                                            >  1:2409721/1‑93 (MQ=255)                                              cgcgGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAAc                                          <  1:4153101/93‑1 (MQ=255)                                                 ggTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGa                                       <  1:200666/93‑1 (MQ=255)                                                   ttGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAAc                                     <  2:4444560/93‑1 (MQ=255)                                                   ctGCAGAAGCTGGCAGCTTAACCCAGGCCGCAGCAAACGCGGATCGCGTGCCGTCGATTCTCAGTGCCCGTCTGCGCGAGCTGGAAACAGAAc                                     <  2:1280981/92‑1 (MQ=255)                                                     gCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTg                                   <  2:2596592/93‑1 (MQ=255)                                                     gCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTg                                   <  1:3345316/93‑1 (MQ=255)                                                     gCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTg                                   <  2:2271198/93‑1 (MQ=255)                                                      ccGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTgg                                  <  1:1392054/93‑1 (MQ=255)                                                       cGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTggg                                 <  2:4280706/93‑1 (MQ=255)                                                       cGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTggg                                 <  1:1906453/93‑1 (MQ=255)                                                          aGCTGGCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGt                              <  1:3288222/93‑1 (MQ=255)                                                             tGGCAGCATAACCCAGGCGGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGa                           <  1:164282/93‑1 (MQ=255)                                                              ggCAGCATAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGAt                          >  1:4053693/1‑93 (MQ=255)                                                               gcagcaTAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATc                         >  1:1732521/1‑93 (MQ=255)                                                                cagcaTAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATCt                        <  2:230734/93‑1 (MQ=255)                                                                  gcaTAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATCTGt                      >  1:11917/1‑93 (MQ=255)                                                                  gcaTAACCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGATGATCTGt                      >  2:1138477/1‑93 (MQ=255)                                                                      aaCCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTTATCTGTTTAt                  <  1:2910994/93‑1 (MQ=255)                                                                      aaCCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATCTGTTTAt                  <  2:1147819/93‑1 (MQ=255)                                                                       aCCCAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATCTGTTTAtt                 <  1:3836161/93‑1 (MQ=255)                                                                        cccAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATCTGTTTATTc                <  1:1043487/93‑1 (MQ=255)                                                                        cccAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATCTGTTTATTc                >  1:1307003/1‑93 (MQ=255)                                                                        cccAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATCTGTTTATTc                <  1:3593920/93‑1 (MQ=255)                                                                        cccAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATCTGTTTATTc                <  2:2552601/93‑1 (MQ=255)                                                                        cccAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATCTGTTTATTc                >  2:1286400/1‑93 (MQ=255)                                                                         ccAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGGAACAGAACTGGGGGGTGATCTGTTTATTcg               >  2:1219338/1‑93 (MQ=255)                                                                         ccAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATCTGTTTATTcg               >  1:675489/1‑93 (MQ=255)                                                                         ccAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATCTGTTTATTcg               >  2:157333/1‑93 (MQ=255)                                                                          cAGGCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATCTGTTTATTcgc              <  2:793140/93‑1 (MQ=255)                                                                            ggCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATCTGTTTATTCGCga            <  1:747986/93‑1 (MQ=255)                                                                            ggCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATCTGTTTATTCGCga            >  2:1691812/1‑93 (MQ=255)                                                                            ggCTGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATCTGTTTATTCGCga            <  2:3010091/93‑1 (MQ=255)                                                                               tGCAGCAAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATCTGTTTATTCGCGAGaa         <  1:3419793/93‑1 (MQ=255)                                                                                gcagcaAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATCTGTTTATTCGCGAGAAt        >  1:2648195/1‑93 (MQ=255)                                                                                 cagcaAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATCTGTTTATTCGCGAGAATc       <  1:4428285/93‑1 (MQ=255)                                                                                  agcaAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATCTGTTTATTCGCGAGAATCa      <  2:3326870/93‑1 (MQ=255)                                                                                   gcaAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATCTGTTTATTCGCGAGAATCAg     >  1:1344364/1‑93 (MQ=255)                                                                                   gcaAAAGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATCTGTTTATTCGCGAGAATCAg     >  2:784114/1‑93 (MQ=255)                                                                                      aaaGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATCTGTTTATTCGCGAGAATCAGCGt  >  2:1039491/1‑93 (MQ=255)                                                                                      aaaGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATCTGTTTATTCGCGAGAATCAGCGt  >  2:1894150/1‑93 (MQ=255)                                                                                      aaaGCGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATCTGTTTATTCGCGAGAATCAGCGt  <  2:2646213/93‑1 (MQ=255)                                                                                          |                                                                                         ACGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATCTGTTTATTCGCGAGAATCAGCGT  >  NC_000913/1612812‑1612989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑