Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,632,141	T→C	S166P (TCA→CCA)	b1545 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,632,141	0	T	C	100.0%	110.0 / NA	40	S166P (TCA→CCA)	b1545	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/20);  total (20/20)

AGTAAGAGCCCGCGGCGCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGTCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAAGAGCCAAAGC  >  NC_000913/1632053‑1632211                                                                                         |                                                                       aGTAAGAGCCCGCGGCGCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAgg                                                                    <  1:1826757/93‑1 (MQ=255) aGTAAGAGCCCGCGGCGCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAgg                                                                    <  1:4570377/93‑1 (MQ=255)  gTAAGAGCCCGCGGCGCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGgt                                                                   >  1:4086798/1‑93 (MQ=37)   tAAGAGCCCGCGGCGCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGgtg                                                                  >  1:4416510/1‑93 (MQ=37)     agagCCGGCGGCGCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTa                                                                <  2:916883/93‑1 (MQ=255)        gCCCGCGGCGCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGt                                                             >  1:3256250/1‑93 (MQ=37)          ccGCGGCGCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGtat                                                           >  1:2653980/1‑93 (MQ=255)           cgcgGCGCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGtata                                                          <  1:2832809/93‑1 (MQ=255)            gcggcgCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATaa                                                         >  2:2700119/1‑93 (MQ=255)            gcggcgCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAACCAGGTGTAAGTATaa                                                         >  2:4645470/1‑93 (MQ=255)              ggcgCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATGAGt                                                       <  1:3217851/93‑1 (MQ=255)                cgcAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGc                                                     >  1:2251407/1‑93 (MQ=255)                  cAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCa                                                   <  1:3137752/93‑1 (MQ=255)                   aGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCAt                                                  <  2:3937221/93‑1 (MQ=255)                   aGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCAt                                                  <  1:2256467/93‑1 (MQ=255)                     ggAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTg                                                >  1:3728802/1‑93 (MQ=255)                         aCGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCg                                            >  1:4679230/1‑93 (MQ=255)                           gTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGCTGTAAGTATAAGTGCCATTGCCCggg                                          >  2:3141480/1‑93 (MQ=255)                            ttttGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGa                                         >  1:1539723/1‑93 (MQ=255)                               tGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAAtt                                      <  2:3635897/93‑1 (MQ=255)                               tGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAAtt                                      <  2:3431848/93‑1 (MQ=255)                               tGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAAtt                                      <  1:2819031/93‑1 (MQ=37)                                          cTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTcgc                           <  1:3827900/93‑1 (MQ=18)                                           tgtgTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTcgcg                          >  2:1451254/1‑93 (MQ=21)                                            gtgtTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCgg                         <  2:2864566/93‑1 (MQ=18)                                                  aaTGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAAcc                   <  2:2592072/93‑1 (MQ=18)                                                  aaTGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAAcc                   >  1:271243/1‑93 (MQ=21)                                                     gaagaaCAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCAtt                >  2:430911/1‑93 (MQ=18)                                                       agaaCAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCAtttt              >  2:2276557/1‑93 (MQ=18)                                                        gaaCAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTa             <  2:993842/93‑1 (MQ=18)                                                           cAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAaga          <  2:1796601/93‑1 (MQ=18)                                                           cAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAaga          <  1:4350642/93‑1 (MQ=18)                                                           cAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAaga          <  1:2696152/93‑1 (MQ=18)                                                            aGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAagag         >  1:3661466/1‑93 (MQ=18)                                                             gAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAAGAGc        <  2:1330580/93‑1 (MQ=18)                                                             gAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAAGAGc        >  1:609445/1‑93 (MQ=21)                                                             gAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAAGAGc        >  2:429563/1‑93 (MQ=21)                                                               aaCAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAAGAGCCa      >  1:977748/1‑93 (MQ=21)                                                               aaCAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAAGAGCCa      >  1:1077105/1‑93 (MQ=21)                                                                aCAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAAGAGCCaa     <  2:1999337/93‑1 (MQ=18)                                                                   ggCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAAGAGCCAAAGc  <  2:4624597/93‑1 (MQ=18)                                                                                         |                                                                       AGTAAGAGCCCGCGGCGCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGTCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAAGAGCCAAAGC  >  NC_000913/1632053‑1632211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑