BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,643,455	T→C	D68G (GAC→GGC)	relE ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,643,455	0	T	C	95.6%	323.9 / 4.5	113	D68G (GAC→GGC)	relE	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (2/3); new base C (50/58); total (52/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01

Only 100 of 115 total aligned reads displayed.

TGGTTCAGAGAATGCGTTTGACCGCCTCGCTATATACTTCCGAGCGTTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGTCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAATC  >  NC_000913/1643366‑1643544

tGGTTCAGAGAATGCGTTTGACCGCCTCGCTATATACTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCta                                                                                        <  1:1716094/93‑1 (MQ=255)

tGGTTCAGAGAATGCGTTTGACCGCCTCGCTATATACTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCta                                                                                        >  1:584879/1‑93 (MQ=255)

 ggTTCAGAGAATGCGTTTGACCGCCTCGCTATATACTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCtat                                                                                       <  2:2991989/93‑1 (MQ=255)

      agagAATGCGTTTGACCGCCTCGCTATATACTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCt                                                                                  <  2:4242230/93‑1 (MQ=255)

      agagAATGCGTTTGACCGCCTCGCTATATACTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCt                                                                                  <  2:2066192/93‑1 (MQ=255)

       gagaATGCGTTTGACCGCCTCTCTATATACTGCCGAGCATGCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTg                                                                                 <  1:2933362/93‑1 (MQ=255)

       gagaATGCGTTTGACCGCCTCGCTATATACTTCCGAGCATTCACTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTg                                                                                 >  1:4660833/1‑93 (MQ=255)

        agaATGCGTTTGACCGCCTCGCTATATACTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTgg                                                                                <  2:3655687/93‑1 (MQ=255)

         gaATGCGTTTGACCGCCTCGCTATATACTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGt                                                                               >  2:3880529/1‑93 (MQ=255)

         gaATGCGTTTGACCGCCTCGCTATATACTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGt                                                                               <  1:1967223/93‑1 (MQ=255)

         gaATGCGTTTGACCGCCTCGCTATATACTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGt                                                                               >  1:4116517/1‑93 (MQ=255)

          aaTGCGTTTGACCGCCTCGCTATATACTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGta                                                                              >  2:3896687/1‑93 (MQ=255)

          aaTGCGTTTGACCGCCTCGCTATATACTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGta                                                                              <  1:3929355/93‑1 (MQ=255)

          aaTGCGTTTGACCGCCTCGCTATATACTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGta                                                                              <  1:3443345/93‑1 (MQ=255)

           aTGCGTTTGACCGCCTCGCTATATACTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGtat                                                                             <  1:1349125/93‑1 (MQ=255)

            tGCGTTTGACCGCCTCGCTATATACTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGTCTATAACCTGGtata                                                                            <  1:2106848/93‑1 (MQ=255)

             gCGTTTGACCGCCTCGCTATATACTTCCGAGCGTTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGTCTATAACCTGGTATAc                                                                           >  1:1685752/1‑93 (MQ=255)

                tttGACCGCCTCGCTATATACTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAg                                                                        >  2:1294816/1‑93 (MQ=255)

                tttGACCGCCTCGCTATATACTACCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACCACAACTTTCTCGCCTATAACCTGGTATACAAg                                                                        <  2:3508689/93‑1 (MQ=255)

                  tGACCGCCTCGCTATATACTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGc                                                                      >  1:3902187/1‑93 (MQ=255)

                   gACCGCCTCGCTATATACTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCg                                                                     <  1:3232116/93‑1 (MQ=255)

                       gCCTCGCTATATACTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAg                                                                 <  1:1519175/93‑1 (MQ=255)

                       gCCTCGCTATATACTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAg                                                                 >  1:3351899/1‑93 (MQ=255)

                       gCCTCGCTATATACTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAg                                                                 >  2:4227718/1‑93 (MQ=255)

                        ccTCGCTATATACTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGc                                                                >  2:1776617/1‑93 (MQ=255)

                         cTCGCTATATACTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGcc                                                               <  2:3496557/93‑1 (MQ=255)

                         cTCGCTATATACTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGcc                                                               >  1:3310784/1‑93 (MQ=255)

                         cTCGCTATATACTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGcc                                                               <  1:3384606/93‑1 (MQ=255)

                          tCGCTATATACTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCt                                                              <  1:102594/93‑1 (MQ=255)

                          tCGCTATATACTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCt                                                              <  1:2859119/93‑1 (MQ=255)

                          tCGCTATATACTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCt                                                              >  2:3581188/1‑93 (MQ=255)

                            gCTATATACTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGa                                                            >  2:86400/1‑93 (MQ=255)

                            gCTATATACTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGa                                                            <  1:3737437/93‑1 (MQ=255)

                              tatataCTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAg                                                          >  2:18386/1‑93 (MQ=255)

                               atataCTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGTCTATAACCTGGTATACAAGGCGATAGCCTGAAGa                                                         <  1:3848363/93‑1 (MQ=255)

                                tataCTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGAc                                                        >  2:1156931/1‑93 (MQ=255)

                                  taCTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCg                                                      <  2:4308115/93‑1 (MQ=255)

                                   aCTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCgg                                                     >  1:3095254/1‑93 (MQ=255)

                                   aCTTCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCgg                                                     <  1:4004909/93‑1 (MQ=255)

                                     ttCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAg                                                   >  2:3013187/1‑93 (MQ=255)

                                     ttCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAg                                                   >  1:431093/1‑93 (MQ=255)

                                      tCCGAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGc                                                  <  1:3952764/93‑1 (MQ=255)

                                         gAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTa                                               >  1:4166680/1‑93 (MQ=255)

                                         gAGCATTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTa                                               >  1:2800822/1‑93 (MQ=255)

                                           gcattCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGGCCGGAGCTTAAt                                             <  1:1150971/90‑1 (MQ=255)

                                           gcattCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAAt                                             >  1:2712739/4‑93 (MQ=255)

                                           gcattCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAAt                                             >  1:3003748/4‑93 (MQ=255)

                                           gcattCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAAt                                             <  1:2884904/90‑1 (MQ=255)

                                            cattCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATc                                            <  1:4120293/91‑1 (MQ=255)

                                             attCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCt                                           >  2:801686/2‑93 (MQ=255)

                                              ttCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCtt                                          >  2:3316962/1‑93 (MQ=255)

                                              ttCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCtt                                          >  1:3641536/1‑93 (MQ=255)

                                               tctcTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTg                                         >  2:3014693/1‑93 (MQ=255)

                                                ctctTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGt                                        >  2:3325269/1‑93 (MQ=255)

                                                ctctTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGt                                        <  1:2133202/93‑1 (MQ=255)

                                                ctctTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGt                                        >  2:1026785/1‑93 (MQ=255)

                                                  ctTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTaa                                      >  1:915879/1‑93 (MQ=255)

                                                  ctTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTaa                                      <  1:3226781/93‑1 (MQ=255)

                                                  ctTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTaa                                      <  1:4201134/93‑1 (MQ=255)

                                                   ttttCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAAc                                     >  1:3443607/1‑93 (MQ=255)

                                                     ttCCCAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACaa                                   <  2:1237802/93‑1 (MQ=255)

                                                        ccAACAGAAATCACGAAAACGACAACTTTCTCGTCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCa                                >  2:2003529/1‑93 (MQ=255)

                                                        ccAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCa                                <  2:1094306/93‑1 (MQ=255)

                                                         cAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAg                               <  2:3819610/93‑1 (MQ=255)

                                                         cAACAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAg                               <  1:3605136/93‑1 (MQ=255)

                                                          accAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAgg                              <  1:1086596/91‑1 (MQ=255)

                                                          aaCAGAAATCACGAAACCGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAgg                              <  2:749090/93‑1 (MQ=255)

                                                           aCAGAAATCACGAAAACGACAACTATCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGCCCGGAGCTTAATCTTGTAACAATCAGGc                             <  2:4597159/93‑1 (MQ=255)

                                                            cAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCa                            <  2:1728033/93‑1 (MQ=255)

                                                            cAGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCa                            <  2:1783186/93‑1 (MQ=255)

                                                             aGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCAt                           >  1:2325888/1‑93 (MQ=255)

                                                             aGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCAt                           <  1:4397875/93‑1 (MQ=255)

                                                             aGAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCAt                           <  2:439780/93‑1 (MQ=255)

                                                             aGAAATCACGAAAACGACAACTTTATCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCAt                           >  1:3644569/1‑93 (MQ=255)

                                                              gAAATCACGAAACCGACAGCTTTCTCGCCTATAACCTGGTATACAAGGCGATAGGCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATa                          <  2:2030872/93‑1 (MQ=255)

                                                              gAAATCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATa                          >  1:3643161/1‑93 (MQ=255)

                                                               aaaTCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATAc                         >  1:1039910/1‑93 (MQ=255)

                                                                aaTCACGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATAcc                        >  2:1940620/1‑93 (MQ=255)

                                                                 aTCACGAAAACGACACCTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCa                       <  1:1268096/93‑1 (MQ=255)

                                                                    aCGAAAACGACAACTTTCTCGTCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACgg                    <  1:3973841/93‑1 (MQ=255)

                                                                    aCGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACgg                    >  2:4807/1‑93 (MQ=255)

                                                                    aCGAAAACGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACgg                    >  2:1565119/1‑93 (MQ=255)

                                                                        aaaCGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCt                >  2:3410237/1‑93 (MQ=255)

                                                                        aaaCGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCt                >  1:68256/1‑93 (MQ=255)

                                                                         aaCGACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCtt               >  2:35744/1‑93 (MQ=255)

                                                                            gACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGtt            <  2:2761998/93‑1 (MQ=255)

                                                                            gACAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGtt            <  2:1732775/93‑1 (MQ=255)

                                                                             aCAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGttt           >  2:2218741/1‑93 (MQ=255)

                                                                              cAACTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTg          >  1:4482361/1‑93 (MQ=255)

                                                                               agcTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGc         <  1:3439678/91‑1 (MQ=255)

                                                                               aaCTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGc         <  1:3773122/93‑1 (MQ=255)

                                                                               aaCTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGc         <  1:802111/93‑1 (MQ=255)

                                                                                aCTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCt        >  1:1096955/1‑93 (MQ=255)

                                                                                aCTTTCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCt        <  1:3155027/93‑1 (MQ=255)

                                                                                  tttCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTc      <  2:2089901/93‑1 (MQ=255)

                                                                                   ttCTCGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCa     <  2:2205764/93‑1 (MQ=255)

                                                                                    tctcGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCaa    <  1:4080470/93‑1 (MQ=255)

                                                                                    tctcGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCaa    >  2:2347842/1‑93 (MQ=255)

                                                                                     ctcGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAAt   >  2:552736/1‑93 (MQ=255)

                                                                                      tcGCCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAATc  >  2:3637625/1‑93 (MQ=255)

TGGTTCAGAGAATGCGTTTGACCGCCTCGCTATATACTTCCGAGCGTTCTCTTTTCCCAACAGAAATCACGAAAACGACAACTTTCTCGTCTATAACCTGGTATACAAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAATC  >  NC_000913/1643366‑1643544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`