BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	148,229	A→G	R189R (CGT→CGC)	panC ←	pantothenate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	148,229	0	A	G	97.1%	517.3 / 0.8	174	R189R (CGT→CGC)	panC	pantothenate synthetase
Reads supporting (aligned to +/- strand): ref base A (3/2); new base G (95/74); total (98/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 179 total aligned reads displayed.

TTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCG  >  NC_000913/148139‑148318

ttCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCg                                                                                         >  1:3224496/1‑93 (MQ=255)

 tCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCgg                                                                                        >  1:2949117/1‑93 (MQ=255)

   cccAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGa                                                                                      >  1:3355509/1‑93 (MQ=255)

     cAGCCTGTAATTTGTCAGCATTCGAACTTAAGACTATGTACAGGCCAGGCGCAATTTTGCGTTGTCCCGCCGTCAGATAGCCGTTGCGGGAAc                                                                                    <  2:2235330/93‑1 (MQ=255)

     cAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAAc                                                                                    <  1:3023033/93‑1 (MQ=255)

      aGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACt                                                                                   >  2:667050/1‑93 (MQ=255)

        ccTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTa                                                                                 <  1:3554787/93‑1 (MQ=255)

        ccTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTa                                                                                 <  2:2488362/93‑1 (MQ=255)

        ccTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTa                                                                                 <  1:2974196/93‑1 (MQ=255)

        ccTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTa                                                                                 >  2:1208060/1‑93 (MQ=255)

         cTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAg                                                                                >  1:1756339/1‑93 (MQ=255)

         cTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAg                                                                                >  1:1083291/1‑93 (MQ=255)

         cTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAg                                                                                <  2:2508739/93‑1 (MQ=255)

         cTGCAATTTGTCAGCAATCGAACTCAAAACTTTGTACAGACCAGGAGCAATTTTGCTTTCTTTCGCCGTGAGATAACCGTTACCGCAACTCAg                                                                                >  1:2284619/1‑93 (MQ=255)

          tGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAgc                                                                               <  2:3341386/93‑1 (MQ=255)

          tGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAgc                                                                               >  2:4306277/1‑93 (MQ=255)

          tGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAgc                                                                               >  2:4090196/1‑93 (MQ=255)

           gCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAgcg                                                                              >  2:3238439/1‑93 (MQ=255)

           gCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAgcg                                                                              >  1:3867517/1‑93 (MQ=255)

            cAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAgcgc                                                                             <  2:4159740/93‑1 (MQ=255)

                 tGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGAc                                                                        <  1:4179438/93‑1 (MQ=255)

                  gTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGAcc                                                                       >  1:945541/1‑93 (MQ=255)

                   tCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCg                                                                      >  1:1207093/1‑93 (MQ=255)

                    cAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGt                                                                     >  2:4637529/1‑93 (MQ=255)

                     aGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTc                                                                    >  2:3519313/1‑93 (MQ=255)

                      gCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCt                                                                   >  1:1850126/1‑93 (MQ=255)

                      gCAATCGAACTTAAAACTTTGTACAGACCAGGCGAAATTTTGCGTTGTGCCGCTGTCAGATAACCGTTGCGGGAACTTAGCGCCCGACCGTCt                                                                   <  2:4571872/93‑1 (MQ=255)

                       cAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCtt                                                                  <  1:3427452/93‑1 (MQ=255)

                        aaTCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCttt                                                                 >  2:1378729/1‑93 (MQ=255)

                         aTCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTg                                                                >  1:4043933/1‑93 (MQ=255)

                          tCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGTACTTAGCGCCAGACCGTCTTTgg                                                               >  2:2742482/1‑93 (MQ=255)

                           cGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGgc                                                              <  2:23010/93‑1 (MQ=255)

                           cGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGgc                                                              <  2:3040877/93‑1 (MQ=255)

                           cGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGgc                                                              <  1:1660623/93‑1 (MQ=255)

                            gAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGgcg                                                             >  2:2004160/1‑93 (MQ=255)

                             aaCTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGgcgc                                                            <  2:3852322/93‑1 (MQ=255)

                             aaCTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGgcgc                                                            >  1:1717913/1‑93 (MQ=255)

                             aaCTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGgcgc                                                            >  1:793725/1‑93 (MQ=255)

                              aCTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGgcgcg                                                           >  2:2424756/1‑93 (MQ=255)

                               cTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGgcgcgc                                                          >  1:3808680/1‑93 (MQ=255)

                               cTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGgcgcgc                                                          >  2:3139872/1‑93 (MQ=255)

                                 tAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCAt                                                        >  2:553468/1‑93 (MQ=255)

                                   aaaCTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATaa                                                      >  2:2847628/1‑93 (MQ=255)

                                   aaaCTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATaa                                                      >  2:985483/1‑93 (MQ=255)

                                    aaCTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAAt                                                     <  2:803556/93‑1 (MQ=255)

                                    aaCTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAAt                                                     <  2:4647929/93‑1 (MQ=255)

                                    aaCTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAAt                                                     >  1:3012119/1‑93 (MQ=255)

                                     aCTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAAtt                                                    <  1:3326647/93‑1 (MQ=255)

                                      cTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTg                                                   <  2:1729645/93‑1 (MQ=255)

                                         tGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGca                                                <  1:2691448/93‑1 (MQ=255)

                                          gTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGcac                                               >  1:2987472/1‑93 (MQ=255)

                                           tACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGcaca                                              >  2:3460730/1‑93 (MQ=255)

                                            aCAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCacac                                             <  2:2602327/93‑1 (MQ=255)

                                             cAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACAcc                                            <  2:1723176/93‑1 (MQ=255)

                                                aCCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGAc                                         >  1:2113843/1‑93 (MQ=255)

                                                 ccAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACa                                        >  1:690661/1‑93 (MQ=255)

                                                 ccAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACCGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACa                                        >  2:2138931/1‑93 (MQ=255)

                                                    ggCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAAtc                                     >  1:3288539/1‑93 (MQ=255)

                                                    ggCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAAtc                                     <  2:2995153/93‑1 (MQ=255)

                                                    ggCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAAtc                                     >  1:364971/1‑93 (MQ=255)

                                                    ggCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAAtc                                     >  1:519100/1‑93 (MQ=255)

                                                     gcgcAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAAtct                                    >  2:1563797/1‑93 (MQ=255)

                                                      cgcAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACCAtctc                                   >  2:1289241/1‑93 (MQ=255)

                                                      cgcAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAAtctc                                   <  2:2938161/93‑1 (MQ=255)

                                                          aTTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAata                               >  1:2512454/1‑93 (MQ=255)

                                                          aTTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAata                               >  1:2228326/1‑93 (MQ=255)

                                                          aTTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAata                               >  1:104078/1‑93 (MQ=255)

                                                            tttGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATc                             <  2:4368841/93‑1 (MQ=255)

                                                              tGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGa                           >  2:3721460/1‑93 (MQ=255)

                                                               tcGTGGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGaa                          <  2:686176/92‑1 (MQ=255)

                                                                cGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAg                         >  2:1266770/1‑93 (MQ=255)

                                                                 gttgttCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGc                        >  1:3092257/1‑93 (MQ=255)

                                                                 gttgttCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGc                        <  1:4334007/93‑1 (MQ=255)

                                                                  ttgttCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGcc                       >  2:915515/1‑93 (MQ=255)

                                                                  ttgttCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGcc                       >  2:3377670/1‑93 (MQ=255)

                                                                   tgttCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGccc                      >  1:382086/1‑93 (MQ=255)

                                                                     ttCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCat                    <  1:2260505/93‑1 (MQ=255)

                                                                      tCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCata                   <  1:4427900/93‑1 (MQ=255)

                                                                       ccgccgTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCatat                  >  1:1302252/1‑93 (MQ=255)

                                                                       ccgccgTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCatat                  <  1:1742195/93‑1 (MQ=255)

                                                                       ccgccgTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCatat                  <  2:733130/93‑1 (MQ=255)

                                                                       ccgccgTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCatat                  <  1:1015924/93‑1 (MQ=255)

                                                                         gccgTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCg                >  2:2292081/1‑93 (MQ=255)

                                                                           cgTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCgga              >  2:3746993/1‑92 (MQ=255)

                                                                           cgTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGc              >  1:880185/1‑93 (MQ=255)

                                                                            gTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCa             >  1:1621325/1‑93 (MQ=255)

                                                                            gTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCa             >  1:252583/1‑93 (MQ=255)

                                                                             tCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCaa            <  1:2613096/93‑1 (MQ=255)

                                                                             tCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCaa            <  2:4237998/93‑1 (MQ=255)

                                                                               aGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAAcc          >  1:4402475/1‑93 (MQ=255)

                                                                               aGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAAcc          >  2:1442130/1‑93 (MQ=255)

                                                                               aGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAAcc          >  1:3442359/1‑93 (MQ=255)

                                                                                 aTAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCAt        <  1:811533/93‑1 (MQ=255)

                                                                                 aTAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCAt        <  2:3824863/93‑1 (MQ=255)

                                                                                  tAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCAtt       <  2:345272/93‑1 (MQ=255)

                                                                                  tAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCAtt       >  1:4575302/1‑93 (MQ=255)

                                                                                    ccggTTGCGGGAACTTAGAGCAAGACCGTCTTTGGCGCGCATAATTGGCACACCGAAAACTCCACTATCGAGGCCCATATCGGCAACAAtttt     >  2:3859493/4‑93 (MQ=255)

                                                                                    aCCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCAtttt     >  1:1245500/1‑93 (MQ=255)

                                                                                    aCCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCAtttt     <  1:4232062/93‑1 (MQ=255)

                                                                                     ccGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTg    <  1:3730800/93‑1 (MQ=255)

TTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCG  >  NC_000913/148139‑148318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`