BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,655,245	T→C	L91L (TTA→TTG)	ynfC ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,655,245	0	T	C	98.5%	400.8 / ‑4.5	129	L91L (TTA→TTG)	ynfC	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (2/0); new base C (72/55); total (74/55)
Fisher's exact test for biased strand distribution p-value = 5.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

TAAACGTACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGTAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAG  >  NC_000913/1655155‑1655333

tAAACGTACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCaa                                                                                        <  1:689335/93‑1 (MQ=255)

  aaCGTACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAAtt                                                                                      <  1:2558261/93‑1 (MQ=255)

    cGTACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGAGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCACCAATTCg                                                                                    >  2:207236/1‑93 (MQ=255)

      tACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAg                                                                                  >  2:3539755/1‑93 (MQ=255)

       aCTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGt                                                                                 >  1:1834657/1‑93 (MQ=255)

          ctctTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGaa                                                                              <  2:1115929/93‑1 (MQ=255)

           tcttctCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAAt                                                                             >  1:2236834/1‑93 (MQ=255)

           tcttctCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAAt                                                                             <  2:4196439/93‑1 (MQ=255)

           tcttctCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAAt                                                                             >  2:473101/1‑93 (MQ=255)

           tcttctCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAAt                                                                             <  1:1623211/93‑1 (MQ=255)

            cttctCCAGCGTCGCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATc                                                                            <  2:1721766/93‑1 (MQ=255)

              tctcCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCaa                                                                          <  2:3166803/93‑1 (MQ=255)

                tcCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCaaaa                                                                        >  2:719891/1‑93 (MQ=255)

                tcCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCaaaa                                                                        >  2:861361/1‑93 (MQ=255)

                 ccAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAAc                                                                       >  1:4526203/1‑93 (MQ=255)

                  cAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACa                                                                      >  1:409659/1‑93 (MQ=255)

                     cGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGcc                                                                   <  2:1897596/93‑1 (MQ=255)

                      gTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCct                                                                  <  1:4237520/93‑1 (MQ=255)

                      gTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCct                                                                  <  2:3123845/93‑1 (MQ=255)

                      gTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCct                                                                  <  2:3413598/93‑1 (MQ=255)

                        ctcGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCcttc                                                                <  1:1107453/93‑1 (MQ=255)

                           ggCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCttcttc                                                             <  2:1949605/93‑1 (MQ=255)

                           ggCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCttcttc                                                             >  2:1062408/1‑93 (MQ=255)

                            gCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGTAATTCGAGTGAATCAAAACAGCCTTCTTcc                                                            >  2:4641373/1‑93 (MQ=255)

                            gCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTcc                                                            >  1:2199446/1‑93 (MQ=255)

                            gCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTcc                                                            <  1:2846944/93‑1 (MQ=255)

                              aTCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCCCGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGa                                                          <  1:548242/93‑1 (MQ=255)

                              aTCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGa                                                          >  2:872967/1‑93 (MQ=255)

                              aTCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGa                                                          >  2:3052219/1‑93 (MQ=255)

                              aTCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGa                                                          >  1:4491536/1‑93 (MQ=255)

                               tCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGAc                                                         >  2:3267039/1‑93 (MQ=255)

                               tCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGAc                                                         >  1:1259764/1‑93 (MQ=255)

                               tCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGAc                                                         >  1:1233958/1‑93 (MQ=255)

                                 atggTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACaa                                                       <  1:1332865/91‑1 (MQ=255)

                                 aCGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACaa                                                       >  1:4105317/1‑93 (MQ=255)

                                  cGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACaaa                                                      <  2:2916182/93‑1 (MQ=255)

                                  cGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACaaa                                                      >  2:3618972/1‑93 (MQ=255)

                                  cGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACaaa                                                      >  1:4286912/1‑93 (MQ=255)

                                   ggTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAg                                                     >  2:2275620/1‑93 (MQ=255)

                                   ggTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAg                                                     <  1:2382711/93‑1 (MQ=255)

                                    gTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGt                                                    <  2:1827935/93‑1 (MQ=255)

                                      aataatTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTcc                                                  <  1:243955/93‑1 (MQ=255)

                                      aataatTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTcc                                                  >  1:379160/1‑93 (MQ=255)

                                      aataatTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTcc                                                  >  2:3225419/1‑93 (MQ=255)

                                      aataatTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTcc                                                  <  1:3233901/93‑1 (MQ=255)

                                       ataatTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTccc                                                 <  1:38367/93‑1 (MQ=255)

                                          atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGa                                              <  2:3139998/93‑1 (MQ=255)

                                          atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGa                                              >  1:2044351/1‑93 (MQ=255)

                                            tGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGaaa                                            <  2:566353/93‑1 (MQ=255)

                                             ggCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAac                                           <  1:308665/93‑1 (MQ=255)

                                               cGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAacac                                         <  1:3809011/93‑1 (MQ=255)

                                                 tCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGt                                       >  2:4416646/1‑93 (MQ=255)

                                                 tCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGt                                       <  2:4081930/93‑1 (MQ=255)

                                                 tCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGt                                       >  2:967809/1‑93 (MQ=255)

                                                 tCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGt                                       <  2:3712194/93‑1 (MQ=255)

                                                 tCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGt                                       >  1:2603304/1‑93 (MQ=255)

                                                  ccAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGtt                                      <  1:1612676/93‑1 (MQ=255)

                                                   cAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGttt                                     <  2:2918584/93‑1 (MQ=255)

                                                    aGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTc                                    >  1:1916282/1‑93 (MQ=255)

                                                    aGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTc                                    <  1:4497168/93‑1 (MQ=255)

                                                     gTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACATTTCg                                   >  1:1560566/1‑93 (MQ=255)

                                                       aCCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTc                                 >  2:467712/1‑93 (MQ=255)

                                                        ccAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCa                                >  1:4346211/1‑93 (MQ=255)

                                                        ccAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCa                                <  1:1536146/93‑1 (MQ=255)

                                                         cAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCAc                               <  2:1557412/93‑1 (MQ=255)

                                                          agagCGAGCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACt                              >  2:3803091/1‑93 (MQ=255)

                                                          agagCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACt                              >  1:322195/1‑93 (MQ=255)

                                                           gagCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACtt                             >  1:1702563/1‑93 (MQ=255)

                                                           gagCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACtt                             >  1:4051833/1‑93 (MQ=255)

                                                           gagCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGGCCCAGAAACACGTTTCGTCACtt                             >  2:3065466/1‑93 (MQ=255)

                                                               gACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCAcc                         >  2:1252526/1‑93 (MQ=255)

                                                                aCCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCt                        >  1:4635174/1‑93 (MQ=255)

                                                                  cACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTg                      >  2:1635496/1‑93 (MQ=255)

                                                                   actgTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGc                     <  1:1152588/90‑1 (MQ=255)

                                                                   aCGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAAGAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGc                     >  1:2512265/1‑93 (MQ=255)

                                                                   aCGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACATCCTTCTCCCGACAAAGTCCCAGAAACACTTTTCGTTACTTCCCCTTGc                     <  2:4574453/93‑1 (MQ=255)

                                                                   aCGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGc                     <  2:4217847/93‑1 (MQ=255)

                                                                    cGGTATTATTTTCCAGATCCAGTAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCt                    >  2:3416837/1‑93 (MQ=255)

                                                                    cGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCt                    <  2:497031/93‑1 (MQ=255)

                                                                     ggTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTc                   >  1:3473566/1‑93 (MQ=255)

                                                                     ggTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTc                   >  1:4411361/1‑93 (MQ=255)

                                                                     ggTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTc                   >  1:3512826/1‑93 (MQ=255)

                                                                       tattatTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCAt                 >  1:2293732/1‑93 (MQ=255)

                                                                       tattatTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCAt                 >  2:3701761/1‑93 (MQ=255)

                                                                       tattatTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCAt                 >  2:735920/1‑93 (MQ=255)

                                                                        attattTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATc                >  2:3978632/1‑93 (MQ=255)

                                                                         ttattTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATcc               >  2:2220056/1‑93 (MQ=255)

                                                                            ttttCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCAtt            <  1:1353725/93‑1 (MQ=255)

                                                                            ttttCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCAtt            <  1:1503476/93‑1 (MQ=255)

                                                                            ttttCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCAtt            <  2:1417837/93‑1 (MQ=255)

                                                                              ttCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTaa          <  1:1324619/93‑1 (MQ=255)

                                                                                 cAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGt       <  2:4041248/93‑1 (MQ=255)

                                                                                  aGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTc      <  2:1566876/93‑1 (MQ=255)

                                                                                   gATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCt     >  2:1249202/1‑93 (MQ=255)

                                                                                   gATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCt     >  2:4226453/1‑93 (MQ=255)

                                                                                   gATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCt     <  2:2942087/93‑1 (MQ=255)

                                                                                   gATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCt     >  1:336868/1‑93 (MQ=255)

                                                                                    aTCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTg    >  1:1579790/1‑93 (MQ=255)

                                                                                     tCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGa   >  1:4649857/1‑93 (MQ=255)

                                                                                      ccAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAg  >  1:3640598/1‑93 (MQ=255)

TAAACGTACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGTAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAG  >  NC_000913/1655155‑1655333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`