Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,662,601 T→C A27A (GCT→GCC)  b1591 → putative oxidoreductase component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,662,6010TC99.2% 365.4 / ‑6.1 117A27A (GCT→GCC) b1591putative oxidoreductase component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (56/60);  minor base A (1/0);  total (57/60)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 122 total aligned reads displayed.

ACATTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGC  >  NC_000913/1662511‑1662690
                                                                                          |                                                                                         
acaTTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGcccc                                                                                         >  1:3874964/1‑93 (MQ=255)
 caTTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCa                                                                                        <  1:242844/93‑1 (MQ=255)
 caTTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCa                                                                                        <  2:1538271/93‑1 (MQ=255)
 caTTTCAGGATGTTAGGAATGAACCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCa                                                                                        >  1:3105267/1‑93 (MQ=255)
  aTTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCag                                                                                       >  2:803358/1‑93 (MQ=255)
   tttCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCaga                                                                                      >  1:4660917/1‑93 (MQ=255)
   tttCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCaga                                                                                      <  1:900067/93‑1 (MQ=255)
   tttCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCaga                                                                                      <  2:1163398/93‑1 (MQ=255)
     tCAGGATGTTAGGAATGACTCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCagaga                                                                                    >  2:4555642/1‑93 (MQ=255)
     tCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCagaga                                                                                    <  1:3341816/93‑1 (MQ=255)
     tCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCagaga                                                                                    <  2:1100735/93‑1 (MQ=255)
     tCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCagaga                                                                                    >  1:4248281/1‑93 (MQ=255)
     tCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCagaga                                                                                    >  1:2865279/1‑93 (MQ=255)
     tCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCagaga                                                                                    >  2:2001897/1‑93 (MQ=255)
       aGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGt                                                                                  <  1:4150962/93‑1 (MQ=255)
       aGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGt                                                                                  >  1:3537149/1‑93 (MQ=255)
       aGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGt                                                                                  <  1:3913073/93‑1 (MQ=255)
       aGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGt                                                                                  >  1:3798484/1‑93 (MQ=255)
       aGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGt                                                                                  >  2:2346967/1‑93 (MQ=255)
       aGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGt                                                                                  >  1:1888451/1‑93 (MQ=255)
         gATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGc                                                                                <  2:2466567/93‑1 (MQ=255)
           tGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCgg                                                                              >  1:3577913/1‑93 (MQ=255)
            gTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGa                                                                             >  1:2910072/1‑93 (MQ=255)
             ttAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGaa                                                                            <  1:878912/93‑1 (MQ=255)
             ttAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGaa                                                                            <  1:2332779/93‑1 (MQ=255)
             ttAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGaa                                                                            <  1:1807217/93‑1 (MQ=255)
               aGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGc                                                                          <  2:2076856/93‑1 (MQ=255)
               aGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGGTTTATTACGCCCCAGAGAGTGCGGAAGc                                                                          >  1:3956178/1‑93 (MQ=255)
                ggAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGcc                                                                         <  1:63063/93‑1 (MQ=255)
                ggAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGcc                                                                         <  1:1641477/93‑1 (MQ=255)
                   aTGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGcggga                                                                      >  2:1072873/1‑89 (MQ=255)
                   aTGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCa                                                                      >  2:1279693/1‑93 (MQ=255)
                   aTGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCa                                                                      <  1:3758992/93‑1 (MQ=255)
                      aCCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGCAGCCGCACct                                                                   >  2:3789598/1‑93 (MQ=255)
                       cctaTTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACctc                                                                  <  1:3599196/90‑1 (MQ=255)
                       cccATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACctc                                                                  >  1:3232472/1‑93 (MQ=255)
                       cccATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACctc                                                                  <  1:1874462/93‑1 (MQ=255)
                       cccATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACctc                                                                  <  1:2525687/93‑1 (MQ=255)
                        ccATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACctct                                                                 >  1:315797/1‑93 (MQ=255)
                        ccATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACctct                                                                 >  1:2278256/1‑93 (MQ=255)
                         cATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTg                                                                >  1:3716526/1‑93 (MQ=255)
                          aTTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTgg                                                               <  1:821804/93‑1 (MQ=255)
                           tttttCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGCAGCCGCACCTCTGGt                                                              >  1:3502400/1‑93 (MQ=255)
                           tttttCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGt                                                              <  2:1440510/93‑1 (MQ=255)
                           tttttCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGt                                                              <  1:797329/93‑1 (MQ=255)
                                   aGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTAc                                                      <  2:4591916/93‑1 (MQ=255)
                                      aaGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCa                                                   <  2:2679878/93‑1 (MQ=255)
                                         aTAGTTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCa                                                <  1:1541668/93‑1 (MQ=255)
                                         aTAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCa                                                <  2:929448/93‑1 (MQ=255)
                                         aTAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCACCAGAGAGTGCGGAAGACGCACCTCTGGTTGCGGTACTCACCa                                                >  1:1196941/1‑93 (MQ=255)
                                           aaTTTTGCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGt                                              <  2:1506937/93‑1 (MQ=255)
                                              ttttCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGAc                                           <  2:1723016/93‑1 (MQ=255)
                                                ttCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACgg                                         >  2:2460151/1‑93 (MQ=255)
                                                ttCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACgg                                         >  1:4408346/1‑93 (MQ=255)
                                                 tCTGTGGCTGCGCGCGTGTTGGCCGCGCTGTTTTATTACGCCCCAGAGATGGCGGAATCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGc                                        <  2:1502672/93‑1 (MQ=255)
                                                     tCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTggg                                    <  1:3875410/93‑1 (MQ=255)
                                                     tCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTggg                                    >  2:365415/1‑93 (MQ=255)
                                                     tCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGGACTCACCAGTGACGGCTggg                                    >  2:1154088/1‑93 (MQ=255)
                                                       gcgccgCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGaa                                  <  2:4620716/93‑1 (MQ=255)
                                                         ggcgCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAAc                                <  1:2267168/93‑1 (MQ=255)
                                                            gcgcgTGTTGGGCGCGCTGTTTTATTACGCCCGAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCa                             <  2:50013/93‑1 (MQ=255)
                                                            gcgcgTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCa                             >  1:2381124/1‑93 (MQ=255)
                                                            gcgcgTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCa                             <  1:4174190/93‑1 (MQ=255)
                                                             cgcgTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAg                            >  2:4613017/1‑93 (MQ=255)
                                                             cgcgTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAg                            >  1:1387935/1‑93 (MQ=255)
                                                             cgcgTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAg                            <  2:733274/93‑1 (MQ=255)
                                                              gcgTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGt                           <  2:4670598/93‑1 (MQ=255)
                                                               cgTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGCAACTCAGTg                          >  1:2710504/1‑93 (MQ=255)
                                                                gtgtTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTgg                         >  1:1881985/1‑93 (MQ=255)
                                                                gtgtTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTgg                         <  1:3212675/93‑1 (MQ=255)
                                                                 tgtTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGc                        >  1:154647/1‑93 (MQ=255)
                                                                 tgtTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGc                        >  1:2939182/1‑93 (MQ=255)
                                                                 tgtTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGc                        >  2:4527593/1‑93 (MQ=255)
                                                                  tttGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGcc                       <  1:1093736/92‑1 (MQ=255)
                                                                    tGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCctc                     <  2:2793957/93‑1 (MQ=255)
                                                                         gcgcTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCa                >  1:1052644/1‑93 (MQ=255)
                                                                         gcgcTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCa                >  1:244176/1‑93 (MQ=255)
                                                                         gcgcTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCa                >  2:904862/1‑93 (MQ=255)
                                                                          cgcTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAg               >  2:1951008/1‑93 (MQ=255)
                                                                           gcTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGa              >  1:4401702/1‑93 (MQ=255)
                                                                            cTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGaa             <  2:2564486/93‑1 (MQ=255)
                                                                            cTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGaa             >  2:2329683/1‑93 (MQ=255)
                                                                             tGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAg            >  1:910702/1‑93 (MQ=255)
                                                                             tGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAg            >  2:3362885/1‑93 (MQ=255)
                                                                              gTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGc           >  2:386643/1‑93 (MQ=255)
                                                                              gTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGc           >  1:2168148/1‑93 (MQ=255)
                                                                               ttttATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGCAACTCAGTGGCCTCTACCAGAAGCg          >  2:601380/1‑93 (MQ=255)
                                                                               ttttATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCg          >  2:1107991/1‑93 (MQ=255)
                                                                                tttATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGt         >  1:1108158/1‑93 (MQ=255)
                                                                                tttATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGt         >  1:3548174/1‑93 (MQ=255)
                                                                                  tattaCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCa       <  2:3497368/93‑1 (MQ=255)
                                                                                   attaCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCAt      <  1:3938684/93‑1 (MQ=255)
                                                                                   attaCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCAt      >  1:1778134/1‑93 (MQ=255)
                                                                                   attaCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCAt      >  1:2419776/1‑93 (MQ=255)
                                                                                   attaCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCAt      <  1:3780274/93‑1 (MQ=255)
                                                                                   attaCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCAt      >  1:4397494/1‑93 (MQ=255)
                                                                                     tccGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTa    <  2:3637254/91‑1 (MQ=255)
                                                                                      aCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAg   <  1:1217234/93‑1 (MQ=255)
                                                                                      aCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAg   <  2:2430832/93‑1 (MQ=255)
                                                                                       cGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAgc  <  2:833688/93‑1 (MQ=255)
                                                                                          |                                                                                         
ACATTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGC  >  NC_000913/1662511‑1662690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑