| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,689,391 | G→A | intergenic (+7/+219) | ydgA → / ← uidC | hypothetical protein/membrane‑associated protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,689,391 | 0 | G | A | 98.9% | 306.3 / ‑5.1 | 89 | intergenic (+7/+219) | ydgA/uidC | hypothetical protein/membrane‑associated protein |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (38/50); total (38/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.20e-01 | |||||||||||
AATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTT > NC_000913/1689303‑1689480 | aaTGTCGTTAGAAGATTTTTTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCaa < 1:821811/93‑1 (MQ=255)aaTGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCaa > 2:2106621/1‑93 (MQ=255)aaTGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCaa > 1:1993015/1‑93 (MQ=255) aTGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCaaa > 1:3392779/1‑93 (MQ=255) tGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCaaaa < 2:3492816/93‑1 (MQ=255) tGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCaaaa < 1:2088438/93‑1 (MQ=255) gTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAAc < 1:4174054/93‑1 (MQ=255) gTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAAc < 1:4148276/93‑1 (MQ=255) atCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAGCGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAAc < 1:4686501/92‑1 (MQ=255) tCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACg < 2:4180165/93‑1 (MQ=255) cGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGc < 1:2796074/93‑1 (MQ=255) gTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGcc > 1:1933587/1‑93 (MQ=255) ttAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCg > 1:2359489/1‑93 (MQ=255) ttAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCg < 1:596642/93‑1 (MQ=255) ttAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCg < 2:1733563/93‑1 (MQ=255) agaagaTTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGga > 2:686191/1‑93 (MQ=255) agaagaTTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGga < 1:87563/93‑1 (MQ=255) agaagaTTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGga > 2:2454727/1‑93 (MQ=255) gaTTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAAt < 1:2568351/93‑1 (MQ=255) aTTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAAtt > 2:3559689/1‑93 (MQ=255) aTTGTGTTGGCATGGTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAAGTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAAtt < 2:1844257/93‑1 (MQ=255) ttttGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAAttt < 1:859384/93‑1 (MQ=255) ttgttgGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTc > 2:3030877/1‑93 (MQ=255) tgttgGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCa > 2:3043056/1‑93 (MQ=255) tgttgGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCa < 2:2947274/93‑1 (MQ=255) gttgGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCAc < 1:896656/93‑1 (MQ=255) gttgGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCAc < 2:2378424/93‑1 (MQ=255) tgGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTc < 2:2304549/93‑1 (MQ=255) tgCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTcc < 2:2401376/92‑1 (MQ=255) aTGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGc > 1:789407/1‑93 (MQ=255) tGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCg > 2:1021512/1‑93 (MQ=255) tGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCg > 1:1176880/1‑93 (MQ=255) gTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGt > 2:2044542/1‑93 (MQ=255) tttGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGtt < 2:294942/93‑1 (MQ=255) tttGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGtt < 1:258721/93‑1 (MQ=255) ttGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGttt > 1:80849/1‑93 (MQ=255) gCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTc > 1:3769597/1‑93 (MQ=255) cGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCa < 2:2939298/93‑1 (MQ=255) cGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCa < 1:545074/93‑1 (MQ=255) cGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCa > 2:3353975/1‑93 (MQ=255) aTGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATc > 2:2891786/1‑93 (MQ=255) aTGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATc < 1:183516/93‑1 (MQ=255) tGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCt < 2:117066/93‑1 (MQ=255) ctctTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAaaac > 1:307147/1‑93 (MQ=255) tctTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAaaaca > 2:1394129/1‑93 (MQ=255) tctTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAaaaca > 1:1737042/1‑93 (MQ=255) ttAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAaaacaaa < 1:3309102/93‑1 (MQ=255) tAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTGATCTGAaaacaaac < 2:2865311/93‑1 (MQ=255) tAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAaaacaaac > 1:2225452/1‑93 (MQ=255) aaCGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAAcc < 2:3853002/93‑1 (MQ=255) aaCGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAAcc < 1:1438590/93‑1 (MQ=255) gtgccGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCaga < 2:4504595/90‑1 (MQ=255) gTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCaga > 2:4647266/1‑93 (MQ=255) ttCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCagag < 1:999040/93‑1 (MQ=255) tCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAgaga > 2:2472608/1‑93 (MQ=255) tCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTAACTCCGGCGTTTTCATCTGAAAACAAACCAgaga < 1:4172253/93‑1 (MQ=255) ccGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGAt < 2:325733/93‑1 (MQ=255) ccGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGAt < 2:2569714/93‑1 (MQ=255) cGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGAtt > 1:2909737/1‑93 (MQ=255) cGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGAtt < 1:4373062/93‑1 (MQ=255) gCTGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGc < 1:69159/93‑1 (MQ=255) tGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCGCTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCt < 2:839523/93‑1 (MQ=255) tGTACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCt < 2:34613/93‑1 (MQ=255) tACCGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGa < 1:3579194/93‑1 (MQ=255) ccGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATg > 1:3443764/1‑93 (MQ=255) cGGCAATTCCGCAGCAGTACTTCACGACCAATACGCCGGAGACTTTTCGCTCCGGCGTTTTCATCTGAACACAAACCAGAGATTGCCTGATgc < 1:4685579/93‑1 (MQ=255) cGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATgc > 1:1002560/1‑93 (MQ=255) cGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATgc > 1:4478647/1‑93 (MQ=255) cGGCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATgc > 1:3449770/1‑93 (MQ=255) gCAATTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATgcgc > 1:4133079/1‑93 (MQ=255) aaTTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTa < 1:2656442/93‑1 (MQ=255) aTTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTAc < 1:395493/93‑1 (MQ=255) aTTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTAc < 1:4019010/93‑1 (MQ=255) aTTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTAc > 2:4676774/1‑93 (MQ=255) aTTCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTAc > 2:3170817/1‑93 (MQ=255) ttCCGCAGCAGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGAGCTatg > 1:2605296/1‑91 (MQ=255) gcagcaGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTa > 2:2832744/1‑93 (MQ=255) gcagcaGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTa < 1:363962/93‑1 (MQ=255) gcagcaGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTa < 1:3764613/93‑1 (MQ=255) gcagcaGTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTa > 2:2065764/1‑93 (MQ=255) gcagTGATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCa > 1:1015417/1‑93 (MQ=255) gcagTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCa < 2:1503904/93‑1 (MQ=255) gcagTAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCa < 1:4617594/93‑1 (MQ=255) tAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGcc < 2:3392779/93‑1 (MQ=255) tAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGcc < 1:1938632/93‑1 (MQ=255) tAATTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGcc < 2:847776/93‑1 (MQ=255) aaTTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCt > 2:3752367/1‑93 (MQ=255) aTTCACGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTa > 1:3644223/1‑93 (MQ=255) aCGACCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCtt < 1:3616672/93‑1 (MQ=255) | AATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTT > NC_000913/1689303‑1689480 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |