Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,689,553	A→G	intergenic (+169/+57)	ydgA → / ← uidC	hypothetical protein/membrane‑associated protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,689,553	0	A	G	100.0%	237.6 / NA	77	intergenic (+169/+57)	ydgA/uidC	hypothetical protein/membrane‑associated protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/43);  total (34/43)

GCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTC  >  NC_000913/1689463‑1689640                                                                                           |                                                                                        gCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGcg                                                                                       >  1:2764744/1‑93 (MQ=255)  cTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAgcgc                                                                                      <  2:133618/93‑1 (MQ=255)  cTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAgcgc                                                                                      >  2:2622211/1‑93 (MQ=255)     aTCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCa                                                                                   <  1:1021512/93‑1 (MQ=255)       cAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACt                                                                                 <  1:1606300/93‑1 (MQ=255)       cAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACt                                                                                 <  1:2106621/93‑1 (MQ=255)         ggCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACttt                                                                               <  2:789407/93‑1 (MQ=255)         ggCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACttt                                                                               >  1:210924/1‑93 (MQ=255)           ccTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGt                                                                             >  2:1020901/1‑93 (MQ=255)           ccTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGt                                                                             <  2:2244202/93‑1 (MQ=255)           ccTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGt                                                                             <  1:3432930/93‑1 (MQ=255)           ccTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGt                                                                             <  2:4504442/93‑1 (MQ=255)            cTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTc                                                                            >  1:2475819/1‑93 (MQ=255)            cTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAAAAAAAGCGCGCACTTTGTc                                                                            <  2:4553108/93‑1 (MQ=255)              aCCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTcaa                                                                          <  1:2435315/93‑1 (MQ=255)               ccTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTcaac                                                                         <  1:1523451/93‑1 (MQ=255)               ccTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTcaac                                                                         >  1:3154362/1‑93 (MQ=255)               ccTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGCTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTcaac                                                                         >  1:3314652/1‑93 (MQ=255)                cTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTcaaca                                                                        >  1:46265/1‑93 (MQ=255)                 ttttCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTcaacaa                                                                       >  2:757726/1‑93 (MQ=255)                  tttCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAAt                                                                      <  2:1933587/93‑1 (MQ=255)                   ttCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATc                                                                     <  1:2257823/93‑1 (MQ=255)                   ttCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATc                                                                     >  2:2758010/1‑93 (MQ=255)                    tCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCt                                                                    <  2:4355332/93‑1 (MQ=255)                    tCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCt                                                                    <  1:686191/93‑1 (MQ=255)                     ccTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTg                                                                   <  2:2909737/93‑1 (MQ=255)                      cTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGa                                                                  <  1:3633480/93‑1 (MQ=255)                        gCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGaaa                                                                <  1:3512155/93‑1 (MQ=255)                        gCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGaaa                                                                <  1:3559689/93‑1 (MQ=255)                         cAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACCATCTGAAAc                                                               >  2:2807405/1‑93 (MQ=255)                         cAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAAc                                                               <  2:3650609/93‑1 (MQ=255)                          aaCACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACg                                                              >  2:3525961/1‑93 (MQ=255)                           acacTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGc                                                             <  1:516984/93‑1 (MQ=255)                           acacTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGc                                                             >  2:942118/1‑93 (MQ=255)                            cacTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGcc                                                            >  1:4111834/1‑93 (MQ=255)                            cacTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGcc                                                            >  2:2358272/1‑93 (MQ=255)                             acTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCg                                                           >  1:4085925/1‑93 (MQ=255)                                 tGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGgaga                                                       <  1:1407265/93‑1 (MQ=255)                                  gAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGAt                                                      <  2:2225452/93‑1 (MQ=255)                                  gAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGAt                                                      >  2:1332109/1‑93 (MQ=255)                                   aaTTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGAtt                                                     >  1:3017467/1‑93 (MQ=255)                                    aTTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAGTCTGAAACGCCGGAGAttt                                                    <  2:4566958/93‑1 (MQ=255)                                    aTTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGAttt                                                    <  2:766017/93‑1 (MQ=255)                                                ttGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCg                                        <  2:1184466/93‑1 (MQ=255)                                                 tGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGt                                       <  2:3449770/93‑1 (MQ=255)                                                  gTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGtt                                      >  1:2500398/1‑93 (MQ=255)                                                  gTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGtt                                      >  1:3138536/1‑93 (MQ=255)                                                  gTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGtt                                      <  2:4387597/93‑1 (MQ=255)                                                     ggCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTAtt                                   >  1:108089/1‑93 (MQ=255)                                                     ggCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTAtt                                   <  2:1737042/93‑1 (MQ=255)                                                     ggCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTAtt                                   >  1:746776/1‑93 (MQ=255)                                                         ggATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTAtttttt                               >  1:2614352/1‑93 (MQ=255)                                                          gATAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTa                              >  1:4442993/1‑93 (MQ=255)                                                           aTAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTAc                             <  1:3030877/93‑1 (MQ=255)                                                            tAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACt                            >  2:1646376/1‑93 (MQ=255)                                                            tAAGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACt                            >  1:2693585/1‑93 (MQ=255)                                                              aGGCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTc                          >  1:2272509/1‑93 (MQ=255)                                                               ggCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCa                         <  2:1101372/93‑1 (MQ=255)                                                               ggCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCa                         <  2:985938/93‑1 (MQ=255)                                                               ggCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCa                         >  2:2557266/1‑93 (MQ=255)                                                               ggCGTTTACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCa                         <  1:2832744/93‑1 (MQ=255)                                                                   tttACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCAt                     >  1:1707971/1‑93 (MQ=255)                                                                   tttACGCCGCATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCAt                     <  1:3890094/93‑1 (MQ=255)                                                                        gccgcATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGt                >  1:4597677/1‑93 (MQ=255)                                                                        gccgcATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGt                <  1:4417712/93‑1 (MQ=255)                                                                         ccgcATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTc               <  2:1152998/93‑1 (MQ=255)                                                                          cgcATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCa              >  2:355051/1‑93 (MQ=255)                                                                           gcATCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCAt             <  1:4676774/93‑1 (MQ=255)                                                                             aTCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATAc           <  2:1370436/93‑1 (MQ=255)                                                                             aTCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATAc           >  2:3658616/1‑93 (MQ=255)                                                                              tCCGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACt          >  2:3980994/1‑93 (MQ=255)                                                                                cGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGa        <  1:3462506/93‑1 (MQ=255)                                                                                cGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGa        >  2:221948/1‑93 (MQ=255)                                                                                cGGCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGa        <  2:195423/93‑1 (MQ=255)                                                                                 ggCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGaa       <  2:1993015/93‑1 (MQ=255)                                                                                  gCATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAAt      <  1:3176207/93‑1 (MQ=255)                                                                                   cATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATa     >  2:3922589/1‑93 (MQ=255)                                                                                   cATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATa     >  1:3239727/1‑93 (MQ=255)                                                                                   cATAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATa     <  1:2065764/93‑1 (MQ=255)                                                                                    aTAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATAc    <  1:1772949/93‑1 (MQ=255)                                                                                     tAAAAAGCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACt   >  1:4292436/1‑93 (MQ=255)                                                                                      aaaaagCGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTc  <  2:2435645/93‑1 (MQ=255)                                                                                           |                                                                                        GCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTC  >  NC_000913/1689463‑1689640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑