BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,697,536	T→C	V53A (GTC→GCC)	malX →	MalX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,697,536	0	T	C	95.5%	307.6 / 4.6	110	V53A (GTC→GCC)	malX	MalX
Reads supporting (aligned to +/- strand): ref base T (2/3); new base C (56/49); total (58/52)
Fisher's exact test for biased strand distribution p-value = 6.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.07e-01

Only 100 of 111 total aligned reads displayed.

ATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGTTCTGTA  >  NC_000913/1697445‑1697625

aTGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTc                                                                                          <  1:1683273/93‑1 (MQ=255)

 tGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGccc                                                                                         <  1:2990811/93‑1 (MQ=255)

   ttACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTg                                                                                       <  2:1672167/93‑1 (MQ=255)

    tACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTgg                                                                                      >  2:749588/1‑93 (MQ=255)

     tcccGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTggg                                                                                     <  1:3567007/92‑1 (MQ=255)

       ccGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCa                                                                                   <  2:2178416/93‑1 (MQ=255)

         gTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAAc                                                                                 >  2:3060897/1‑93 (MQ=255)

          tGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAAcc                                                                                >  2:1531257/1‑93 (MQ=255)

           ggCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAAccc                                                                               >  2:3153138/1‑93 (MQ=255)

           ggCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAAccc                                                                               >  1:4675622/1‑93 (MQ=255)

           ggCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGACATGATGTCATAACCCTGATCCCGGCCCTGGGCAAccc                                                                               >  1:2346936/1‑93 (MQ=255)

               ttattGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCgtg                                                                           >  1:2882209/1‑93 (MQ=255)

               ttattGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCgtg                                                                           >  2:1493183/1‑93 (MQ=255)

                tattGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCgtgt                                                                          <  1:1310804/93‑1 (MQ=255)

                tattGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCgtgt                                                                          <  1:4666255/93‑1 (MQ=255)

                 attGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGtt                                                                         >  1:1197277/1‑93 (MQ=255)

                  ttGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAAACCCGTGTTg                                                                        >  2:1855701/1‑93 (MQ=255)

                   tGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGc                                                                       <  1:735173/93‑1 (MQ=255)

                    gTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCa                                                                      <  2:3380864/93‑1 (MQ=255)

                    gTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCa                                                                      >  2:201069/1‑93 (MQ=255)

                    gTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCa                                                                      <  2:1672993/93‑1 (MQ=255)

                     tCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAg                                                                     >  2:3707496/1‑93 (MQ=255)

                     tCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAg                                                                     <  1:5965/93‑1 (MQ=255)

                     tCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAg                                                                     >  2:1229055/1‑93 (MQ=255)

                       gTTCTTCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGc                                                                   >  1:3975980/1‑93 (MQ=255)

                       gTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGc                                                                   >  2:3645088/1‑93 (MQ=255)

                       gTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGc                                                                   >  2:946590/1‑93 (MQ=255)

                        ttCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCt                                                                  >  2:1646594/1‑93 (MQ=255)

                         tCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTa                                                                 <  1:1336428/93‑1 (MQ=255)

                           tGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATc                                                               >  2:2665200/1‑93 (MQ=255)

                            gCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCt                                                              >  2:4444808/1‑93 (MQ=255)

                             cGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCtt                                                             <  2:721777/93‑1 (MQ=255)

                             cGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCtt                                                             >  2:1219761/1‑93 (MQ=255)

                              ggCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCttt                                                            >  2:2745354/1‑93 (MQ=255)

                               gCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTa                                                           >  2:238797/1‑93 (MQ=255)

                               gCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTa                                                           >  1:3163394/1‑93 (MQ=255)

                                cATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTTATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTAc                                                          <  1:2408078/93‑1 (MQ=255)

                                 aTTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTAcc                                                         >  1:2974169/1‑93 (MQ=255)

                                 aTTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTAcc                                                         >  2:4322621/1‑93 (MQ=255)

                                 aTTATGCTCGGCATTGGTAGGTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTAcc                                                         >  1:3592843/1‑93 (MQ=255)

                                  ttATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCt                                                        >  1:3151424/1‑93 (MQ=255)

                                   tATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTg                                                       <  2:3462484/93‑1 (MQ=255)

                                    aTGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTgg                                                      >  2:2778416/1‑93 (MQ=255)

                                     tGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGa                                                     >  2:1098482/1‑93 (MQ=255)

                                     tGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGa                                                     <  1:1041089/93‑1 (MQ=255)

                                     tGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGa                                                     <  1:2177112/93‑1 (MQ=255)

                                      gCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTCCCTGGAt                                                    <  2:3802802/93‑1 (MQ=255)

                                        tCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGa                                                  >  2:4320203/1‑93 (MQ=255)

                                         cGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAg                                                 <  1:3929283/93‑1 (MQ=255)

                                            cATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTaa                                              >  2:3841786/1‑93 (MQ=255)

                                            cATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTaa                                              >  1:3100196/1‑93 (MQ=255)

                                              ttGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGa                                            <  2:2025296/93‑1 (MQ=255)

                                              ttGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGa                                            >  2:4677976/1‑93 (MQ=255)

                                              ttGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGa                                            <  1:2987935/93‑1 (MQ=255)

                                              ttGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGGGTTGCAGGCTATCTTTACCTGGATTAGTaaaa                                            >  2:2386998/1‑91 (MQ=255)

                                               tGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGAt                                           <  2:1126543/93‑1 (MQ=255)

                                                ggTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGAtt                                          <  1:3973957/93‑1 (MQ=255)

                                                 gTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTg                                         >  1:3454208/1‑93 (MQ=255)

                                                 gTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTg                                         >  1:3077552/1‑93 (MQ=255)

                                                   aGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCTGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGc                                       <  2:2705522/93‑1 (MQ=255)

                                                    gTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCt                                      <  1:1338301/93‑1 (MQ=255)

                                                    gTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCt                                      <  1:2414882/93‑1 (MQ=255)

                                                     ttCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTc                                     <  2:1351043/93‑1 (MQ=255)

                                                     ttCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTc                                     <  1:239304/93‑1 (MQ=255)

                                                     ttCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTc                                     >  2:2945956/1‑93 (MQ=255)

                                                     ttCTCTGAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTc                                     <  2:1351994/93‑1 (MQ=255)

                                                      tctcTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTcc                                    >  1:2574156/1‑92 (MQ=255)

                                                      tctcTTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCg                                    <  2:4521758/93‑1 (MQ=255)

                                                       ctctTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGt                                   <  1:219264/93‑1 (MQ=255)

                                                       ctctTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGt                                   <  1:1322829/93‑1 (MQ=255)

                                                        tctTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGtt                                  <  1:393154/93‑1 (MQ=255)

                                                        tctTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGtt                                  <  1:1134304/93‑1 (MQ=255)

                                                         ctTAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGttt                                 <  1:203802/93‑1 (MQ=255)

                                                          ttAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTCGGCTACCCCGGGTTGCAGGCTATCTTTACCTGGATCAGTAACATTCGCTCCttta                                >  2:2114491/1‑92 (MQ=255)

                                                           tAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGc                               <  1:593686/93‑1 (MQ=255)

                                                           tAGCAGCCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGc                               >  1:1642267/1‑93 (MQ=255)

                                                             gcagcCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGCTTGGtt                             >  2:2823246/1‑93 (MQ=255)

                                                              cagcCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCttt                            <  1:4262389/93‑1 (MQ=255)

                                                               agcCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCtttt                           <  1:812162/93‑1 (MQ=255)

                                                               agcCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCtttt                           <  2:3287712/93‑1 (MQ=255)

                                                                gcCATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGGttttt                          >  2:18240/1‑92 (MQ=255)

                                                                  cATGATGTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGt                        <  2:1532979/93‑1 (MQ=255)

                                                                   atgatgTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGtt                       <  1:2578499/93‑1 (MQ=255)

                                                                   atgatgTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGCTTTCTTTTTGtt                       >  1:821003/1‑93 (MQ=255)

                                                                     gatgTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTc                     <  2:2119869/93‑1 (MQ=255)

                                                                      atgTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTcc                    <  1:700536/93‑1 (MQ=255)

                                                                       tgTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTcct                   <  1:3489823/93‑1 (MQ=255)

                                                                       tgTCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTcct                   >  2:1083365/1‑93 (MQ=255)

                                                                         tCATAACCCTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTtcttc                 >  2:3180955/1‑91 (MQ=255)

                                                                                ccTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTTGTTTCCTGCCTGTCAt          >  2:3413074/1‑93 (MQ=255)

                                                                                ccTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTCAt          >  2:4653563/1‑93 (MQ=255)

                                                                                 cTGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTCATg         >  1:3051140/1‑93 (MQ=255)

                                                                                  tGATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGGTTGCCTTTTGGTTTCTGcctgtcctgt        >  2:2753565/1‑93 (MQ=255)

                                                                                   gATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGtt       <  2:1605742/93‑1 (MQ=255)

                                                                                   gATCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGtt       <  2:4006020/93‑1 (MQ=255)

                                                                                    aTCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGTTc      <  2:2305386/93‑1 (MQ=255)

                                                                                     tCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTTCTGCCTTTGATGttgt     >  1:4047930/1‑91 (MQ=255)

                                                                                     tCCCGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGGTTGCTTTTAGTTTTCTGCCTGTCATTTTCt     >  2:1060080/1‑93 (MQ=255)

                                                                                       ccGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTTCTGCCTGTCATGTTCTGt   >  2:1807887/1‑93 (MQ=255)

                                                                                        cGGCCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGTTCTGTa  <  1:1582850/93‑1 (MQ=255)

ATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGTTCTGTA  >  NC_000913/1697445‑1697625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`