Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,738,433	T→C	F167L (TTT→CTT)	ydhC →	putative transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,738,433	0	T	C	100.0%	232.4 / NA	75	F167L (TTT→CTT)	ydhC	putative transport protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/42);  total (33/42)

CGCTGGTGGGTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGTTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGACC  >  NC_000913/1738344‑1738519                                                                                          |                                                                                       cGCTGGTGGGTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTg                                                                                     <  1:4247184/93‑1 (MQ=255)   cTGGTGGGTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGcc                                                                                   >  1:1149531/1‑93 (MQ=255)    tggtggGTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCa                                                                                  <  2:4244920/93‑1 (MQ=255)     ggtgggtgTATGTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGTGGCGGGGCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCAt                                                                                 <  2:2051091/93‑1 (MQ=255)     ggtggGTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCAt                                                                                 >  1:3803042/1‑93 (MQ=255)     ggtggGTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCAt                                                                                 <  1:2479255/93‑1 (MQ=255)     ggtggGTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCAt                                                                                 <  2:4659647/93‑1 (MQ=255)     ggtggGTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCAt                                                                                 <  2:4233966/93‑1 (MQ=255)      gtggGTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCAtt                                                                                <  1:2038682/93‑1 (MQ=255)      gtggGTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCAtt                                                                                <  1:2038681/93‑1 (MQ=255)       tggGTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTa                                                                               >  2:999928/1‑93 (MQ=255)        gggTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTAc                                                                              <  1:2019643/93‑1 (MQ=255)        gggTCTATCTCCGCCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCCCCCTGCTTGCCATTAc                                                                              <  2:3275337/93‑1 (MQ=255)             tATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCgtgg                                                                         >  2:1467669/1‑93 (MQ=255)             tATCTCCGGCACTGGCTCCTCAGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGGCACCCTGCTTGCCATTCCCgtgg                                                                         >  2:3469300/1‑93 (MQ=255)               tctcCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCgtggtg                                                                       >  1:128589/1‑93 (MQ=255)                   cGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGa                                                                   <  1:4002381/93‑1 (MQ=255)                   cGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTAGCCACCCTGCTTGCCATTACCGTGGTGCTGa                                                                   >  2:2610184/1‑93 (MQ=255)                    ggCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGAt                                                                  <  2:3456414/93‑1 (MQ=255)                         tGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGc                                                             >  1:4245354/1‑93 (MQ=255)                          ggCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGTCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGcc                                                            >  2:2475874/1‑93 (MQ=255)                           gCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCt                                                           >  1:1991539/1‑93 (MQ=255)                                tctGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTAtttt                                                      >  1:3999777/1‑93 (MQ=255)                                  tGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCt                                                    >  2:974611/1‑93 (MQ=255)                                  tGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCt                                                    >  2:3894901/1‑93 (MQ=255)                                   gTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTg                                                   <  1:3780779/93‑1 (MQ=255)                                   gTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTg                                                   >  2:220831/1‑93 (MQ=255)                                       ggAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTc                                               >  2:1097165/1‑93 (MQ=255)                                         aaGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCaa                                             <  2:1739974/93‑1 (MQ=255)                                          aGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCaaa                                            <  2:2079158/93‑1 (MQ=255)                                          aGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCaaa                                            >  1:1340823/1‑93 (MQ=255)                                          aGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCaaa                                            >  2:3351543/1‑93 (MQ=255)                                          aGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCaaa                                            <  2:50035/93‑1 (MQ=255)                                          aGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCaaa                                            >  1:2066349/1‑93 (MQ=255)                                           gctggctgCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAAc                                           >  1:771652/1‑93 (MQ=255)                                            ctggctgCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAAcc                                          >  2:1138221/1‑93 (MQ=255)                                              ggctgCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCa                                        <  1:4171120/93‑1 (MQ=255)                                               gctgctGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCac                                       >  2:2651100/1‑93 (MQ=255)                                               gctgctGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCac                                       >  2:3231159/1‑93 (MQ=255)                                                ctgctgGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCacg                                      >  1:1198543/1‑93 (MQ=255)                                                 tgctgGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCacga                                     <  1:3439687/93‑1 (MQ=255)                                                 tgctgGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGCGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCacga                                     >  2:4375542/1‑93 (MQ=255)                                                    tgGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCAcgacga                                  >  1:1724508/1‑93 (MQ=255)                                                     ggTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGaa                                 <  1:4124859/93‑1 (MQ=255)                                                      gTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCACACCCACGACGAAg                                >  1:4662336/1‑93 (MQ=255)                                                      gTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAg                                <  2:932860/93‑1 (MQ=255)                                                      gTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAg                                <  2:4002583/93‑1 (MQ=255)                                                      gTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAg                                <  2:3303073/93‑1 (MQ=255)                                                       tCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAgg                               <  1:1389930/93‑1 (MQ=255)                                                         cATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGcc                             >  1:2011238/1‑93 (MQ=255)                                                         cATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGcc                             <  1:2727409/93‑1 (MQ=255)                                                         cATTTTTCCTGGAAGGCGATTTTCGCCACCCTGCTTGCCATTAACGTGGTGCTGATTCTGCCTATTTTCTGGATCAAACCCAACACGAAAGcc                             >  2:1353589/1‑93 (MQ=255)                                                          aTTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGccc                            >  2:3998654/1‑93 (MQ=255)                                                          aTTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGccc                            <  2:1915159/93‑1 (MQ=255)                                                           tttttCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCg                           >  2:1252399/1‑93 (MQ=255)                                                               tCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAAc                       <  1:3033523/93‑1 (MQ=255)                                                                ccTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACa                      <  1:1611463/93‑1 (MQ=255)                                                                ccTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACa                      <  1:3029217/93‑1 (MQ=255)                                                                  tGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAAt                    <  2:702515/93‑1 (MQ=255)                                                                  cggCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAAt                    <  2:765017/92‑1 (MQ=255)                                                                   ggCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATa                   <  1:1798241/93‑1 (MQ=255)                                                                   ggCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATa                   <  2:1669179/93‑1 (MQ=255)                                                                   ggCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATa                   >  1:976822/1‑93 (MQ=255)                                                                    gCAGGCGATTTTCGCCTCCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAg                  <  1:184905/93‑1 (MQ=255)                                                                    gCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAg                  <  1:1309903/93‑1 (MQ=255)                                                                    gCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAg                  <  1:541448/93‑1 (MQ=255)                                                                     cAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGt                 <  1:4214588/93‑1 (MQ=255)                                                                      aGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTc                >  1:2746879/1‑93 (MQ=255)                                                                        gCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAg              <  1:873016/93‑1 (MQ=255)                                                                          gATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGa            <  1:358203/93‑1 (MQ=255)                                                                           aTTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGAt           >  1:2604837/1‑93 (MQ=255)                                                                               tCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTc       <  2:3044465/93‑1 (MQ=255)                                                                               tCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTc       <  2:1897118/93‑1 (MQ=255)                                                                                  ccACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGa    <  1:3132708/93‑1 (MQ=255)                                                                                    aCCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGAcc  <  2:2767570/93‑1 (MQ=255)                                                                                    aCCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGAcc  >  1:4071245/1‑93 (MQ=255)                                                                                          |                                                                                       CGCTGGTGGGTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGTTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGACC  >  NC_000913/1738344‑1738519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑