Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,739,307	G→A	intergenic (+161/‑130)	ydhC → / → cfa	putative transport protein/cyclopropane fatty acyl phospholipid synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,739,307	0	G	A	100.0%	264.1 / NA	73	intergenic (+161/‑130)	ydhC/cfa	putative transport protein/cyclopropane fatty acyl phospholipid synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/30);  total (43/30)

CTAATTTCGAGTTGTTAAAGCTACGATAAATATTATGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAGGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGTTTTTTCTGCGAGATTTCTCACAAAGCCCAAAAAGCGTCTACG  >  NC_000913/1739217‑1739395                                                                                           |                                                                                         cTAATTTCGAGTTGTTAAAGCTACGATAAATATTATGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAagg                                                                                        >  2:254392/1‑93 (MQ=255)   aaTTTCGAGTTGTTAAAGCTACGATAAATATTATGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGtt                                                                                      >  2:173930/1‑93 (MQ=255)    aTTTCGAGTTGTTAAAGCTACGATAAATATTATGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTtc                                                                                     <  2:4628187/93‑1 (MQ=255)     tttCGAGTTGTTAAAGCTACGATAAATATTATGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGtttc                                                                                    >  2:711850/1‑91 (MQ=255)     tttCGAGTTGTTAAAGCTACGATAAATATTATGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTtcc                                                                                    <  1:3923527/93‑1 (MQ=255)     tttCGAGTTGTTAAAGCTACGATAAATATTATGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTtcc                                                                                    >  2:3379961/1‑93 (MQ=255)          aGTTGTTAAAGCTACGATAAATATTATGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCtt                                                                               <  1:213423/93‑1 (MQ=255)            ttgttAAAGCTACGATAAATATTATGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTc                                                                             >  2:3453920/1‑93 (MQ=255)             tgttAAAGCTACGATAAATATTATGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTcc                                                                            >  2:2767182/1‑93 (MQ=255)              gttAAAGCTACGATAAATATTATGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTccc                                                                           >  2:1385993/1‑93 (MQ=255)              gttAAAGCTACGATAAATATTATGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTccc                                                                           >  2:2821946/1‑93 (MQ=255)                tAAAGCTACGATAAATATTATGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCctc                                                                         <  2:2690782/93‑1 (MQ=255)                   aGCTACGATAAATATTATGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGt                                                                      >  1:4601123/1‑93 (MQ=255)                    gCTACGATAAATATTATGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGtt                                                                     >  1:4525071/1‑93 (MQ=255)                      tACGATAAATATTATGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCt                                                                   >  2:3634844/1‑93 (MQ=255)                       aCGATAAATATTATGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTa                                                                  <  1:4122853/93‑1 (MQ=255)                       aCGATAAATATTATGTTTTTACGGGGACAGGATCATTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTa                                                                  <  2:3898502/93‑1 (MQ=255)                          aTAAATATTATGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGt                                                               >  2:4621695/1‑93 (MQ=255)                          aTAAATATTATGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGt                                                               >  2:3330947/1‑93 (MQ=255)                            aaaTATTATGTTTTTACGGGGCCAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCg                                                             <  1:3833743/93‑1 (MQ=255)                             aaTATTATGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCgg                                                            >  1:2443140/1‑93 (MQ=255)                             aaTATTATGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCgg                                                            >  1:814392/1‑93 (MQ=255)                             aaTATTATGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCgg                                                            >  1:772455/1‑93 (MQ=255)                              atatTATGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGa                                                           >  2:2069286/1‑93 (MQ=255)                              atatTATGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGa                                                           >  2:57516/1‑93 (MQ=255)                              atatTATGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGa                                                           <  1:3920322/93‑1 (MQ=255)                              atatTATGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGa                                                           >  1:3539620/1‑93 (MQ=255)                              atatTATGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGa                                                           >  1:1870423/1‑93 (MQ=255)                              atatTATGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGa                                                           <  1:1887888/93‑1 (MQ=255)                                attatGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGAtt                                                         >  1:3926504/1‑93 (MQ=255)                                 ttatGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATta                                                        <  2:633733/93‑1 (MQ=255)                                  tatGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATtat                                                       >  2:2491334/1‑93 (MQ=255)                                   atGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATtata                                                      >  2:507702/1‑93 (MQ=255)                                    tGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAg                                                     <  1:150757/93‑1 (MQ=255)                                    tGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAg                                                     >  1:219615/1‑93 (MQ=255)                                    tGTTTTTACGGGGACAGGATAGTTCCCGACTCACTATGGATAGTCACTTCAGCAAAGGTTCCTCCTTTCACTCTGTTCTACATCAGATTTTAg                                                     >  2:2431588/1‑93 (MQ=255)                                      tttttACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGAc                                                   >  1:1690755/1‑93 (MQ=255)                                       ttttACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACt                                                  <  2:1041393/93‑1 (MQ=255)                                         ttACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTcg                                                <  1:1518626/93‑1 (MQ=255)                                         ttACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTcg                                                <  2:4621931/93‑1 (MQ=255)                                            cGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGAATCGCgg                                             >  1:415748/1‑93 (MQ=255)                                             cgggAAAGGATCCTTCCCGACTCACTAAGGATAGGCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGGTCTACGTCGGATTAAAAACTCGCGGt                                            <  2:3750445/92‑1 (MQ=255)                                                 aCAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGttttt                                        >  1:2670362/1‑93 (MQ=255)                                                  cAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGtttttt                                       >  1:836264/1‑93 (MQ=255)                                                   aGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGttttttt                                      >  2:3219187/1‑92 (MQ=255)                                                   aGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGTTTTTTc                                      <  2:2416566/93‑1 (MQ=255)                                                    ggATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGTTTTTTCt                                     <  2:54360/93‑1 (MQ=255)                                                    ggATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGTTTTTTCt                                     <  1:2036230/93‑1 (MQ=255)                                                       tCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACTTCGGATTATAGACTCGCGGTTTTGTCcgcg                                  >  1:1671182/1‑93 (MQ=255)                                                       tCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGTTTTTTCTGCg                                  >  2:4308528/1‑93 (MQ=255)                                                       tCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGTTTTTTCTGCg                                  <  2:4369662/93‑1 (MQ=255)                                                        cGTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGTTTTTTCTGCga                                 <  1:131806/93‑1 (MQ=255)                                                         gTTCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGTTTTTTCTTCgcg                                >  2:3326569/1‑91 (MQ=255)                                                          ttCCCGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGTTTTTTCTGCgaga                               >  1:541437/1‑93 (MQ=255)                                                            cccGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGTTTTTTCTGCGAGAtt                             <  1:3028260/93‑1 (MQ=255)                                                             ccGACTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGTTTTTTCTGCGAGAttt                            <  1:908801/93‑1 (MQ=255)                                                              ccaCTCACTATGGATAGTCAGTTCGGCAACGGTTCCTCCTTCCCCTCTGTTCTACGCCGGATTATAGACTCGCGGTTTTTTCTGCGAGATTtc                           <  2:4118147/91‑1 (MQ=255)                                                                aCTCACTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGTTTTTTCTGCGAGATTtctc                         <  2:1144695/93‑1 (MQ=255)                                                                    aCTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGTTTTTTCTGCGAGATTTCTCACaa                     >  2:4659698/1‑93 (MQ=255)                                                                     cTATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGTTTTTTCTGCGAGATTTCTCACaaa                    <  1:3138866/93‑1 (MQ=255)                                                                      tATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGTTTTTTCTGCGAGATTTCTCACAAAg                   >  1:4493856/1‑93 (MQ=255)                                                                      tATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGTTTTTTCTGCGAGATTTCTCACAAAg                   >  1:3913383/1‑93 (MQ=255)                                                                      tATGGATAGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGTTTTTTCTGCGAGATTTCTCACAAAg                   <  1:3579665/93‑1 (MQ=255)                                                                             aGTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGTTTTTTCTGCGAGATTTCTCACAAAGCCCaaaa            >  2:936212/1‑93 (MQ=255)                                                                              gTCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGTTTTTTCTGCGAGATTTCTCACAAAGCCCaaaaa           >  2:559626/1‑93 (MQ=255)                                                                               tCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGTTTTTTCTGCGAGATTTCTCACAAAGCCCAAAAAg          <  2:3029289/93‑1 (MQ=255)                                                                               tCATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGTTTTTTCTGCGAGATTTCTCACAAAGCCCAAAAAg          >  2:1888147/1‑93 (MQ=255)                                                                                cATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGTTTTTTCTGCGAGATTTCTCACAAAGCCCAAAAAGc         >  2:1151310/1‑93 (MQ=255)                                                                                cATTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGTTTTTTCTGCGAGATTTCTCACAAACCCCCAAAAGc         >  2:1989567/1‑93 (MQ=255)                                                                                 aTTTCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGTTTTTTCTGCGAGATTTCTCACAAAGCCCAAAAAGCg        >  1:2855973/1‑93 (MQ=255)                                                                                  tttCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGTTTTTTCTGCGAGATTTCTCACAAAGCCCAAAAAGCGt       >  2:4235966/1‑93 (MQ=255)                                                                                   ttCGGCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGTTTTTTCTGCGAGATTTCTCACAAAGCCCAAAAAGCGTc      <  1:1667427/93‑1 (MQ=255)                                                                                      ggCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGTTTTTTCTGCGAGATTTCTCACAAAGCCCAAAAAGCGTCTAc   <  1:3962782/93‑1 (MQ=255)                                                                                      ggCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGTTTTTTCTGCGAGATTTCTCACAAAGCCCAAAAAGCGTCTAc   <  1:1368945/93‑1 (MQ=255)                                                                                       gCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGTTTTTTCTGCGAGATTTCTTACCAAGCCCAAAAAGCGTCTAcc  >  2:4000000/1‑92 (MQ=255)                                                                                       gCAAAGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGTTTTTTCTGCGAGATTTCTCACAAAGCCCAAAAAGCGTCTACg  <  1:3979780/93‑1 (MQ=255)                                                                                           |                                                                                         CTAATTTCGAGTTGTTAAAGCTACGATAAATATTATGTTTTTACGGGGACAGGATCGTTCCCGACTCACTATGGATAGTCATTTCGGCAAGGGTTCCTCCTTTCCCTCTGTTCTACGTCGGATTATAGACTCGCGGTTTTTTCTGCGAGATTTCTCACAAAGCCCAAAAAGCGTCTACG  >  NC_000913/1739217‑1739395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑